MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B97
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1505 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/89(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.370e+00815 (16)33.016.914.040.7 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
23.690e+00412 (13)20.013.5-6.1330.1 80625/7.3HUMANProtein-glutamine gamma-glutamyltransferase 3-like (Transglutaminase 3-like) (TGase 3-like)
32.789e+00411 (12)17.012.415.940.7 59519/5.1HUMANKeratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
41.976e+00412 (13)17.013.5-2.0750.2 84032/5.6HUMANHyaluronan mediated motility receptor (Intracellular hyaluronic acid binding protein) (Receptor for hyaluronan-mediated motility) (CD168 antigen)
51.921e+0046 (6)26.06.78.4061.1 33247/5.2HUMANAlpha-soluble NSF attachment protein (SNAP-alpha) (N-ethylmaleimide-sensitive factor attachment protein, alpha)
61.448e+0048 (8)12.09.04.5781.5 83935/6.4HUMANDystrobrevin alpha (Dystrobrevin-alpha)
71.172e+0046 (6)14.06.79.3656.3 55932/5.6HUMANBTB/POZ domain containing protein 2
898628 (8)11.09.01.5571.1 95203/8.9MOUSEGlutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5)
972047 (7)23.07.92.1853.3 44288/8.3HUMANDual specificity mitogen-activated protein kinase kinase 4 (MAP kinase kinase 4) (JNK activating kinase 1) (c-Jun N-terminal kinase kinase 1) (JNKK) (SAPK/ERK kinase 1) (SEK1)
1071267 (7)10.07.9-2.0165.1 91547/5.4MOUSEDNA replication licensing factor MCM3 (DNA polymerase alpha holoenzyme-associated protein P1) (P1-MCM3)
45313...............x..x..x.x..x.xx......xx......x........x.x....x............x...........x...
7149.....x.xx..........x.x........x..............x.x.....x.......x......x......x.............
108237...x.x....x...x.x...........x..x.........x...x............x.................x............
65118x...x...x..............x....x..........x.......x.x.......x..........xx............x......
60059........................................x...............x.x....x...x.......x.............
3064x........................x.........x.........x.......x..x.x..................x...........
22633.....x............x...x............x..........................x.................x........
4713.......x..................................x.x.......x...x........xx..x...................
27278........x..................x.......x.......x.......................x..x..x...............
113034.x...x......................x.........x.........x..........................x....x........


Detailed Results

1. 15/89 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1092.55671092.5034496036160(R) (G)
1127.55881127.5407162892981(K) (V)
1179.61551179.6010123773860(K) (H)
1217.61131217.6166-4.36346440(R) (-)
1277.72601277.7105124734830(K) (T)
1300.58001300.5302382582670(K) (N)
1302.69141302.7018-8.03934031(R) (V)
1302.69141302.7157-193443550(R) (A)
1383.70621383.6909111861970(K) (V)
1393.73401393.73270.952782891(R) (D)
1475.78321475.7494232122230(K) (T)
1475.78321475.7858-1.82002110(R) (W)
1638.83241638.8604-171861991(K) (F)
1688.01571687.97072766821(R) (G)
1716.89111716.8517234184320(K) (G)
1994.03911993.9772312242390(R) (R)
2384.05862383.9524455195490(R) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 33% (217/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/89 matches (13%).
Acc. #: Species: HUMAN Name: Protein-glutamine gamma-glutamyltransferase 3-like (Transglutaminase 3-like) (TGase 3-like)
Index: MW: 80625 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1031.59171031.56382719261(R) (S)
1045.55361045.54646.83823910(R) (E)
1047.54711047.5587-11pyroGlu 4654731(R)QVYSKAVNR(L)
1140.52291140.5425-175855931(K) (K)
1179.61551179.60101285940(K) (G)
1307.70431307.7099-4.26146240(K) (V)
1307.70431307.7323-215425530(R) (K)
1493.77411493.8076-2247591(R) (A)
1526.74691526.7855-251671790(R) (G)
1638.83241638.8339-0.904414551(K) (Y)
1718.90641718.9117-3.1pyroGlu 6947080(R)QLQVDLVSPHFPDIK(G)
1939.98551940.0129-141671831(R) (H)
2091.99042092.0034-6.229460(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 20% (147/719AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/89 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1003.54831003.5536-5.43633701(K) (N)
1031.59171031.5989-6.92582660(R) (A)
1060.53041060.5097191Met-ox 191(-)MSVRYSSSK(H)
1090.51971090.5315-111481560(K) (L)
1106.57081106.5264401Met-ox 1481560(K)VTMQNLNDR(L)
1300.58001300.5956-122862951(K) (N)
1316.63571316.5905341Met-ox 2862951(K)NHEEEMKDLR(N)
1434.80591434.7705254404501(K) (S)
1493.77411493.7348263233341(R) (D)
1707.82991707.77273441590(K) (G)
2097.04702096.9810314234390(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (104/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/89 matches (13%).
Acc. #: Species: HUMAN Name: Hyaluronan mediated motility receptor (Intracellular hyaluronic acid binding protein) (Receptor for hyaluronan-mediated motility) (CD168 antigen)
Index: MW: 84032 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
951.4104951.4172-7.15465520(K) (K)
1009.49971009.48021971791(K) (N)
1047.54711047.5322144754830(K) (A)
1217.61131217.6265-121051140(R) (M)
1300.58001300.5917-9.11Met-ox 3603690(K)LCSFQEEMVK(E)
1427.82851427.8474-132092211(K) (E)
1526.74691526.8007-355795891(K) (T)
1566.78011566.7453226846961(K) (E)
1704.90581704.8478341051181(R) (L)
1939.98551939.9296293093241(R) (F)
1940.99561941.0657-363353501(K) (E)
2294.04672294.1161-303513691(K) (E)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 17% (127/724AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/89 matches (6%).
Acc. #: Species: HUMAN Name: Alpha-soluble NSF attachment protein (SNAP-alpha) (N-ethylmaleimide-sensitive factor attachment protein, alpha)
Index: MW: 33247 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1431.72361431.672436951060(K) (A)
1691.85681691.8072292092220(K) (L)
1707.82991707.8021161Met-ox 2092220(K)AALCHFCIDMLNAK(L)
1765.78911765.8332-2579941(K) (A)
1929.97621929.92032938531(K) (M)
2091.99042092.0636-351811990(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 26% (77/295AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/89 matches (8%).
Acc. #: Species: HUMAN Name: Dystrobrevin alpha (Dystrobrevin-alpha)
Index: MW: 83935 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
951.4104951.4570-491Met-ox 181(-)MIEDSGKR(G)
1235.59601235.6384-343914010(K) (L)
1350.71101350.6541427327430(K) (-)
1493.77411493.7236344955070(R) (A)
1638.83241638.9107-4881940(R) (R)
1691.85681691.8023325265391(K) (R)
1707.82991707.7972191Met-ox 5265391(K)DELEQRMSALQESR(R)
2199.05752198.9689406346530(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (92/743AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 6/89 matches (6%).
Acc. #: Species: HUMAN Name: BTB/POZ domain containing protein 2
Index: MW: 55932 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1031.59171031.5638273573640(R) (C)
1127.55881127.5697-9.74864960(K) (T)
1212.64491212.63379.32973060(R) (R)
1350.71101350.6840202192300(R) (L)
1760.84331760.9046-352002140(K) (K)
2283.26482283.1636441071250(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (75/525AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 8/89 matches (8%).
Acc. #: Species: MOUSE Name: Glutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5)
Index: MW: 95203 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.55361045.5278257517591(K) (S)
1450.71461450.71132.38168270(K) (R)
1475.78321475.7203434204321(R) (T)
1635.75341635.8277-458148271(K) (R)
1691.85681691.9332-452822960(R) (W)
1838.97361838.9396187868010(K) (N)
1851.99341851.9228383874021(K) (I)
1940.99561941.0407-234334480(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (99/836AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 7/89 matches (7%).
Acc. #: Species: HUMAN Name: Dual specificity mitogen-activated protein kinase kinase 4 (MAP kinase kinase 4) (JNK activating kinase 1) (c-Jun N-terminal kinase kinase 1) (JNKK) (SAPK/ERK kinase 1) (SEK1)
Index: MW: 44288 Da pI: 8.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1047.54711047.5627-1546540(K) (S)
1283.67891283.7323-422292390(R) (S)
1350.71101350.6516441Met-ox 3643730(K)HPFILMYEER(A)
1463.74331463.7243132752871(R) (S)
1929.97621929.9784-1.23353500(R) (D)
1952.05721952.0268161912070(K) (I)
1994.03911994.03810.502452621(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 23% (94/399AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/89 matches (7%).
Acc. #: Species: MOUSE Name: DNA replication licensing factor MCM3 (DNA polymerase alpha holoenzyme-associated protein P1) (P1-MCM3)
Index: MW: 91547 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
968.5295968.5165134965030(R) (M)
1031.59171031.5923-0.612582660(R) (Q)
1300.58001300.6425-4882930(K) (Q)
1423.68721423.7334-323023140(R) (K)
1551.88611551.8284373023151(R) (A)
2091.99042092.0271-183723921(R) (L)
2283.26482283.1873345775960(K) (L)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 10% (84/812AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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