MS-Fit Search Results


Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B98
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (20000 - 40000 Da) selects 36332 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 3959 entries.
Pre searches select 3959 entries.

Data Set 1 Results

MS-Fit search selects 611 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/104(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
194448 (7)35.07.744.060.3 31363/4.7HUMANComplement component 1, Q subcomponent binding protein, mitochondrial precursor (Glycoprotein gC1qBP) (GC1q-R protein) (Hyaluronan-binding protein 1) (p32) (p33)
276157 (6)30.06.731.2120 35760/8.9MOUSEDEOXYRIBONUCLEASE GAMMA PRECURSOR (DNASE GAMMA) (DEOXYRIBONUCLEASE I-LIKE 3) (DNASE I HOMOLOGOUS PROTEIN DHP2) (LIVER AND SPLEEN DNASE) (LS-DNASE) (LSD)
357604 (3)32.03.88.6517.0 20418/5.6HUMANADP-ribosylation factor-like protein 1
431986 (5)28.05.85.16116 24453/6.2MOUSERas-related protein Rab-19
527508 (7)37.07.713.565.9 30851/5.8HUMANCarbonyl reductase [NADPH] 3 (NADPH-dependent carbonyl reductase 3)
626539 (8)26.08.75.8469.2 33753/5.4MOUSEMyeloid differentiation primary response protein MyD88
726267 (6)32.06.710.5100 20659/10.3HUMANNucleoside diphosphate kinase, mitochondrial precursor (NDP kinase, mitochondrial) (NDK) (nm23-H4)
816396 (5)23.05.818.859.7 32381/9.6HUMAN60S ribosomal protein L19, mitochondrial precursor (L19mt) (MRP-L15)
912805 (4)10.04.830.5114 36696/6.2MOUSELow affinity immunoglobulin gamma FC region receptor II precursor (FC-gamma RII) (FCRII) (IGG FC receptor II beta) (FC gamma receptor IIB) (FCgammaRIIB)
1011754 (3)23.03.814.2136 27857/8.4HUMANClaudin-18
73008.........................x.........x................x.......x..x.......x......................x......x..
45966.......x............................xx...........................xx...x.....................x...........
7504...............................................................................x.................xxx....
58004..........x................................x...........x.........x......x........x......................
92421......................................x....x...............x....x.x....x............x.x.................
64070....x.........x...........x......x...x..........x..............xx..x....................................
17743.............x..................x.x..x....x............................x..x.............................
4825...................xx......x...x..........x.......................x.....................................
57269........x..........................x...x........................x..x....................................
26823..x...............................x...................................................x.....x...........


Detailed Results

1. 8/104 matches (7%).
Acc. #: Species: HUMAN Name: Complement component 1, Q subcomponent binding protein, mitochondrial precursor (Glycoprotein gC1qBP) (GC1q-R protein) (Hyaluronan-binding protein 1) (p32) (p33)
Index: MW: 31363 Da pI: 4.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.66881179.7036-29AcetN 1101(-)LPLLRCVPR(V)
1283.72171283.65235481910(K) (E)
1513.77351513.6811612082200(R) (D)
1621.82431621.7532441051190(K) (L)
1637.84361637.7481581Met-ox 1051190(K)MSGGWELELNGTEAK(L)
1697.93541697.83865781941(K) (K)
2287.30112287.1709571551740(K) (N)
3150.57983150.4214501812070(K) (E)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 35% (99/282AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/104 matches (6%).
Acc. #: Species: MOUSE Name: DEOXYRIBONUCLEASE GAMMA PRECURSOR (DNASE GAMMA) (DEOXYRIBONUCLEASE I-LIKE 3) (DNASE I HOMOLOGOUS PROTEIN DHP2) (LIVER AND SPLEEN DNASE) (LS-DNASE) (LSD)
Index: MW: 35760 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1014.57601014.4896852562640(R) (A)
1290.71601290.63306487970(R) (L)
1293.66841293.66641.62862961(K) (K)
1650.93391650.8379582182310(K) (K)
1652.84191652.9046-3842551(K) (R)
1696.85531696.9420-512412550(R) (S)
2211.20632210.9891981862040(K) (K)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 30% (93/310AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/104 matches (3%).
Acc. #: Species: HUMAN Name: ADP-ribosylation factor-like protein 1
Index: MW: 20418 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1791.87691791.8839-3.91631780(K) (S)
2352.12102352.0885141281490(K) (D)
2384.10452384.0784112Met-ox 1281490(K)QDMEQAMTSSEMANSLGLPALK(D)
2464.32282464.28901460791(K) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (58/181AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/104 matches (5%).
Acc. #: Species: MOUSE Name: Ras-related protein Rab-19
Index: MW: 24453 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1032.46221032.5301-6631380(K) (S)
1320.67621320.60075768780(K) (F)
1546.77121546.75768.81391510(R) (H)
1650.93391650.8525491641771(K) (E)
1702.74491702.8587-671381511(K) (H)
1838.99631838.9077481031170(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (62/217AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/104 matches (7%).
Acc. #: Species: HUMAN Name: Carbonyl reductase [NADPH] 3 (NADPH-dependent carbonyl reductase 3)
Index: MW: 30851 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1297.76511297.8320-521992101(K) (R)
1320.67621320.6224411871980(R) (L)
1599.88991599.81314859710(R) (A)
1638.84071638.8056211Met-ox 1151(-)MSSCSRVALVTGANR(G)
1652.84191652.8720-1843580(R) (F)
1697.93541697.8831312182321(K) (T)
1940.06501940.03541559741(R) (D)
1963.02731962.9847221581741(R) (F)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 37% (103/277AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 9/104 matches (8%).
Acc. #: Species: MOUSE Name: Myeloid differentiation primary response protein MyD88
Index: MW: 33753 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
987.5977987.572625991061(R) (S)
1045.60371045.54315873810(R) (S)
1194.69371194.6424432832911(K) (A)
1274.65851274.7109-412392500(K) (R)
1293.66841293.6803-9.21811901(R) (L)
1430.73581430.8120-532392511(K) (L)
1637.84361637.824712pyroGlu 1201330(K)QQNQESEKPLQVAR(V)
1638.84071638.8525-7.22182311(R) (E)
1654.87521654.8474171Met-ox 2182311(R)RMVVVVSDDYLQSK(E)
1654.87521654.8513141201330(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 26% (77/296AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 7/104 matches (6%).
Acc. #: Species: HUMAN Name: Nucleoside diphosphate kinase, mitochondrial precursor (NDP kinase, mitochondrial) (NDK) (nm23-H4)
Index: MW: 20659 Da pI: 10.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1041.51651041.48662928370(R) (E)
1260.70621260.7581-4190991(R) (Y)
1282.66871282.7483-6240510(R) (R)
1293.66841293.6890-161111(-) (G)
1309.73781309.6839411Met-ox 1111(-)MGGLFWRSALR(G)
1697.93541697.8281631221380(R) (G)
1713.92511713.8230601Met-ox 1221380(R)AMIGHTDSAEAAPGTIR(G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (60/187AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/104 matches (5%).
Acc. #: Species: HUMAN Name: 60S ribosomal protein L19, mitochondrial precursor (L19mt) (MRP-L15)
Index: MW: 32381 Da pI: 9.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1107.61111107.60505.51711790(R) (D)
1109.58831109.5479361071170(R) (I)
1203.60061203.6275-222212290(R) (G)
1247.75141247.7224231291401(R) (N)
1309.73781309.73025.812230(R) (V)
1652.84191652.7354642482590(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (65/280AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 5/104 matches (4%).
Acc. #: Species: MOUSE Name: Low affinity immunoglobulin gamma FC region receptor II precursor (FC-gamma RII) (FCRII) (IGG FC receptor II beta) (FC gamma receptor IIB) (FCgammaRIIB)
Index: MW: 36696 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1021.47061021.5107-391491560(R) (S)
1283.72171283.649756pyroGlu 2402510(K)QVPALPGNPDHR(E)
1300.64961300.6762-202402510(K) (E)
1638.84071638.7321662522650(R) (Q)
1654.87521654.7270901Met-ox 2522650(R)EMGETLPEEVGEYR(Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 10% (34/330AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 4/104 matches (3%).
Acc. #: Species: HUMAN Name: Claudin-18
Index: MW: 27857 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
969.5116969.4641492272360(K) (N)
1282.66871282.5803692472570(R) (H)
1963.02731962.9826232082260(K) (A)
2211.20632211.3918-84811020(R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (62/261AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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