MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B99
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 100000 Da) selects 108217 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 12424 entries.
Pre searches select 12424 entries.

Data Set 1 Results

MS-Fit search selects 446 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/69(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	4083	7 (10)	8.0	10.1	6.57	60.0		86904/5.7		HUMAN	Integrin beta-7 precursor
2	1171	4 (5)	11.0	5.8	28.8	28.2		45252/5.8		HUMAN	GPI-anchor transamidase (GPI transamidase) (Phosphatidylinositol-glycan biosynthesis, class K protein) (PIG-K) (hGPI8)
3	904	6 (8)	10.0	8.7	3.76	36.2		54152/5.5		HUMAN	Stromelysin-2 precursor (Matrix metalloproteinase-10) (MMP-10) (Transin-2) (SL-2)
4	608	6 (8)	7.0	8.7	28.7	50.1		98602/8.7		MOUSE	ADAM 12 precursor (A disintegrin and metalloproteinase domain 12) (Meltrin alpha)
5	524	6 (8)	13.0	8.7	-13.3	20.2		40041/9.2		HUMAN	Beta-1,4-galactosyltransferase 4 (Beta-1,4-GalTase 4) (Beta4Gal-T4) (b4Gal-T4) (UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4) (UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4) [Includes: N-acetyllactosamine synthase (Nal>

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Detailed Results

1. 7/69 matches (10%).
Acc. #: Species: HUMAN Name: Integrin beta-7 precursor
Index: MW: 86904 Da pI: 5.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
964.4727	964.4740	-1.4		772	779	0	(K) (S)
969.5265	969.5046	23		191	199	0	(R) (T)
1007.4305	1007.4686	-38		790	798	0	(R) (-)
1073.5548	1073.5380	16		764	771	0	(K) (Q)
1073.5548	1073.5955	-38		780	789	0	(K) (F)
1127.6121	1127.5809	28		117	127	0	(R) (V)
1274.7624	1274.7360	21		200	211	0	(K) (L)
2225.3174	2225.2222	43		191	211	1	(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (67/798AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/69 matches (5%).
Acc. #: Species: HUMAN Name: GPI-anchor transamidase (GPI transamidase) (Phosphatidylinositol-glycan biosynthesis, class K protein) (PIG-K) (hGPI8)
Index: MW: 45252 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1127.6121 1127.5632 43 278 287 0 (K) (T)
1707.9419 1707.9070 20 294 308 1 (R) (K)
2352.2405 2352.1514 38 75 95 0 (R) (N)
2384.1737 2384.1412 14 2Met-ox 75 95 0 (R)LGIPDSHIVLMLADDMACNPR(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (46/395AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/69 matches (8%).
Acc. #: Species: HUMAN Name: Stromelysin-2 precursor (Matrix metalloproteinase-10) (MMP-10) (Transin-2) (SL-2)
Index: MW: 54152 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
962.4607 962.4624 -1.7 399 406 0 (K) (Y)
992.4982 992.4954 2.8 356 363 0 (K) (G)
1089.5008 1089.5250 -22 278 288 0 (K) (C)
1090.5832 1090.5573 24 398 406 1 (K) (Y)
1300.6722 1300.6789 -5.1 424 435 0 (R) (V)
1308.7755 1308.7428 25 374 385 0 (R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (52/476AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/69 matches (8%).
Acc. #: Species: MOUSE Name: ADAM 12 precursor (A disintegrin and metalloproteinase domain 12) (Meltrin alpha)
Index: MW: 98602 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
992.4982 992.4624 36 31 38 0 (R) (G)
1115.5416 1115.5638 -20 281 288 0 (R) (K)
1179.6736 1179.6374 31 52 63 0 (K) (D)
1259.7878 1259.7298 46 731 740 1 (K) (K)
1290.7689 1290.7058 49 213 223 0 (K) (E)
2211.3076 2211.2402 30 239 256 1 (R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (67/903AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/69 matches (8%).
Acc. #: Species: HUMAN Name: Beta-1,4-galactosyltransferase 4 (Beta-1,4-GalTase 4) (Beta4Gal-T4) (b4Gal-T4) (UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4) (UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4) [Includes: N-acetyllactosamine synthase (Nal>
Index: MW: 40041 Da pI: 9.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
964.4727 964.4951 -23 62 70 0 (K) (K)
994.4671 994.4780 -11 113 119 0 (R) (A)
1095.6302 1095.6526 -20 274 283 0 (K) (Y)
1111.6339 1111.6410 -6.4 302 310 1 (R) (V)
1127.6121 1127.6359 -21 1Met-ox 302 310 1 (R)MKLLHQVSR(V)
1232.7283 1232.7254 2.4 173 183 0 (K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (46/344AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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