MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B100
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 443 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/83(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.798e+004	9 (10)	29.0	10.8	14.8	32.1		28303/4.8		HUMAN	14-3-3 protein gamma (Protein kinase C inhibitor protein-1) (KCIP-1)
2	1.433e+004	8 (9)	14.0	9.6	11.0	20.2		59519/5.1		HUMAN	Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
3	9588	8 (9)	31.0	9.6	16.0	17.7		27764/4.7		HUMAN	14-3-3 protein tau (14-3-3 protein theta) (14-3-3 protein T-cell) (HS1 protein)
4	8474	8 (9)	24.0	9.6	13.3	22.6		27745/4.7		HUMAN	14-3-3 protein zeta/delta (Protein kinase C inhibitor protein-1) (KCIP-1) (Factor activating exoenzyme S) (FAS)
5	8466	8 (9)	24.0	9.6	13.3	22.6		27771/4.7		MOUSE	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT)

49131	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.
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81689	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.
22922	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.

Detailed Results

1. 9/83 matches (10%).
Acc. #: Species: HUMAN Name: 14-3-3 protein gamma (Protein kinase C inhibitor protein-1) (KCIP-1)
Index: MW: 28303 Da pI: 4.8

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1080.5750	1080.5577	16		133	142	0	(R) (R)
1156.6569	1156.6326	21	AcetN	1	10	1	(-)VDREQLVQK(A)
1189.6921	1189.6615	26		218	227	0	(K) (D)
1205.6797	1205.6564	19	1Met-ox	218	227	0	(K)DSTLIMQLLR(D)
1236.6869	1236.6588	23		133	143	1	(R) (A)
1245.6358	1245.6162	16		163	172	0	(K) (L)
1245.6358	1245.6625	-21		1	10	1	(-) (A)
1261.6436	1261.6112	26	1Met-ox	163	172	0	(K)EHMQPTHPIR(L)
1261.6436	1261.6574	-11	1Met-ox	1	10	1	(-)MVDREQLVQK(A)
1643.8205	1643.7877	20		29	42	0	(K) (N)
2131.0339	2130.9719	29		199	217	0	(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (74/247AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/83 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1090.5243 1090.5315 -6.6 148 156 0 (K) (L)
1109.4978 1109.4904 6.7 335 343 0 (K) (S)
1234.6918 1234.6796 9.9 236 245 1 (R) (Q)
1365.6453 1365.6399 4.0 323 333 0 (R) (K)
1434.7970 1434.7705 19 440 450 1 (K) (S)
1493.7731 1493.7348 26 323 334 1 (R) (D)
1707.8054 1707.7727 19 41 59 0 (K) (G)
2097.0028 2096.9810 10 423 439 0 (R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (87/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/83 matches (9%).
Acc. #: Species: HUMAN Name: 14-3-3 protein tau (14-3-3 protein theta) (14-3-3 protein T-cell) (HS1 protein)
Index: MW: 27764 Da pI: 4.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1124.5644 1124.5523 11 1Met-ox 159 167 0 (K)EMQPTHPIR(L)
1189.6921 1189.6615 26 213 222 0 (K) (D)
1205.6797 1205.6564 19 1Met-ox 213 222 0 (K)DSTLIMQLLR(D)
1236.6869 1236.6523 28 158 167 1 (K) (L)
1320.6809 1320.6800 0.73 104 115 0 (K) (V)
1410.7016 1410.6687 23 128 139 1 (R) (Q)
1532.7422 1532.7193 15 28 41 0 (K) (N)
2143.0529 2143.0308 10 139 157 1 (R) (K)
2143.0529 2143.0308 10 140 158 1 (K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (76/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/83 matches (9%).
Acc. #: Species: HUMAN Name: 14-3-3 protein zeta/delta (Protein kinase C inhibitor protein-1) (KCIP-1) (Factor activating exoenzyme S) (FAS)
Index: MW: 27745 Da pI: 4.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1064.4950 1064.4947 0.28 pyroGlu 76 83 1 (K)QQMAREYR(E)
1124.5644 1124.5523 11 1Met-ox 159 167 0 (K)EMQPTHPIR(L)
1189.6921 1189.6615 26 213 222 0 (K) (D)
1205.6797 1205.6564 19 1Met-ox 213 222 0 (K)DSTLIMQLLR(D)
1236.6869 1236.6523 28 158 167 1 (K) (L)
1548.7482 1548.7142 22 28 41 0 (K) (N)
2041.0153 2040.9878 13 140 157 0 (K) (K)
2169.0826 2169.0828 -0.082 140 158 1 (K) (E)
2169.0826 2169.0828 -0.082 139 157 1 (K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (61/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/83 matches (9%).
Acc. #: Species: MOUSE Name: 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT)
Index: MW: 27771 Da pI: 4.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1064.4950 1064.4947 0.28 pyroGlu 76 83 1 (K)QQMAREYR(E)
1124.5644 1124.5523 11 1Met-ox 159 167 0 (K)EMQPTHPIR(L)
1189.6921 1189.6615 26 213 222 0 (K) (D)
1205.6797 1205.6564 19 1Met-ox 213 222 0 (K)DSTLIMQLLR(D)
1236.6869 1236.6523 28 158 167 1 (K) (L)
1548.7482 1548.7142 22 28 41 0 (K) (N)
2041.0153 2040.9878 13 140 157 0 (K) (K)
2169.0826 2169.0828 -0.082 140 158 1 (K) (E)
2169.0826 2169.0828 -0.082 139 157 1 (K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (61/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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