MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B101
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1151 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/69(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16.665e+00410 (14)34.014.533.082.3 27745/4.7HUMAN14-3-3 protein zeta/delta (Protein kinase C inhibitor protein-1) (KCIP-1) (Factor activating exoenzyme S) (FAS)
21.447e+0049 (13)29.013.032.686.7 27771/4.7MOUSE14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT)
31.095e+00411 (15)18.015.917.872.5 75874/5.4HUMANAnnexin VI (Lipocortin VI) (P68) (P70) (Protein III) (Chromobindin 20) (67 kDa calelectrin) (Calphobindin-II) (CPB-II)
4957613 (18)15.018.81.4173.6 97287/6.7MOUSEGlycogen phosphorylase, muscle form (Myophosphorylase)
5900811 (15)17.015.915.061.2 75887/5.3MOUSEAnnexin VI (Lipocortin VI) (P68) (P70) (Protein III) (Chromobindin 20) (67 kDa calelectrin) (Calphobindin-II) (CPB-II)
81689......................x.....x...........x...x...x....x.x....x....x.x.
22922......................x.....x...........x...x...x....x......x....x.x.
21935..x........x.x......x.........................xxx..x.....xx.x........
19232...................x.....x.x......x.....x...x.x..x....x..xx.x...x....
33876..x........x.x.........x......................x....xx....xx.x....x...



Detailed Results

1. 10/69 matches (14%).
Acc. #: Species: HUMAN Name: 14-3-3 protein zeta/delta (Protein kinase C inhibitor protein-1) (KCIP-1) (Factor activating exoenzyme S) (FAS)
Index: MW: 27745 Da pI: 4.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1064.44891064.4947-43pyroGlu 76831(K)QQMAREYR(E)
1080.54331080.489750pyroGlu 1Met-ox 76831(K)QQMAREYR(E)
1189.70721189.6615382132220(K) (D)
1205.72921205.6564601Met-ox 2132220(K)DSTLIMQLLR(D)
1236.72571236.6523591581671(K) (L)
1279.58471279.6534-541281391(R) (G)
1304.73301304.6850371041150(K) (V)
1548.80071548.71425628410(K) (N)
2041.07632040.9878431401570(K) (K)
2169.20822169.0828581401581(K) (E)
2169.20822169.0828581391571(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (84/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/69 matches (13%).
Acc. #: Species: MOUSE Name: 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT)
Index: MW: 27771 Da pI: 4.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1064.44891064.4947-43pyroGlu 76831(K)QQMAREYR(E)
1080.54331080.489750pyroGlu 1Met-ox 76831(K)QQMAREYR(E)
1189.70721189.6615382132220(K) (D)
1205.72921205.6564601Met-ox 2132220(K)DSTLIMQLLR(D)
1236.72571236.6523591581671(K) (L)
1279.58471279.6534-541281391(R) (G)
1548.80071548.71425628410(K) (N)
2041.07632040.9878431401570(K) (K)
2169.20822169.0828581401581(K) (E)
2169.20822169.0828581391571(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (72/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/69 matches (15%).
Acc. #: Species: HUMAN Name: Annexin VI (Lipocortin VI) (P68) (P70) (Protein III) (Chromobindin 20) (67 kDa calelectrin) (Calphobindin-II) (CPB-II)
Index: MW: 75874 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1008.46121008.4308304474560(K) (A)
1032.46971032.5036-336646730(K) (-)
1039.47241039.4730-0.541Met-ox 6116201(K)SMKGAGTDEK(T)
1055.48211055.4832-1.056630(R) (S)
1055.48211055.5382-535475540(R) (S)
1215.72001215.6810322662761(K) (I)
1217.60011217.5398505105200(R) (E)
1236.72571236.6523591Met-ox 1111(-)MAKPAQGAKYR(G)
1236.72571236.7541-234995091(R) (E)
1261.69441261.6502352973061(K) (N)
1475.82801475.7593471001131(K) (C)
1532.83681532.7531551231350(R) (D)
1548.80071548.7481341Met-ox 1231350(R)TNEQMHQLVAAYK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (127/673AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/69 matches (18%).
Acc. #: Species: MOUSE Name: Glycogen phosphorylase, muscle form (Myophosphorylase)
Index: MW: 97287 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1053.58921053.5693198338411(R) (I)
1053.58921053.5733156436500(R) (V)
1072.49051072.5427-498248320(R) (Q)
1078.48111078.5454-601Met-ox 4294380(R)MSLVEEGAVK(R)
1145.53941145.5631-211621700(R) (I)
1145.53941145.5890-435946021(R) (T)
1189.70721189.6727298018111(R) (F)
1205.72921205.6676511Met-ox 8018111(R)MVIRNIATSGK(F)
1215.72001215.65855118290(R) (K)
1243.63421243.6395-4.3AcetN 1111(-)SRPLSDQDKR(K)
1282.60361282.6392-288058161(R) (T)
1475.82801475.7535513603711(R) (T)
1532.83681532.8008242362481(R) (A)
1548.80071548.79573.31Met-ox 2362481(R)NNVVNTMRLWSAK(A)
1941.05391941.0856-165765901(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (133/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 11/69 matches (15%).
Acc. #: Species: MOUSE Name: Annexin VI (Lipocortin VI) (P68) (P70) (Protein III) (Chromobindin 20) (67 kDa calelectrin) (Calphobindin-II) (CPB-II)
Index: MW: 75887 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1008.46121008.4308304474560(K) (T)
1032.46971032.5036-336646730(K) (-)
1039.47241039.4730-0.541Met-ox 6116201(K)SMKGAGTDEK(T)
1069.50061069.49881.756630(R) (S)
1215.72001215.6810322662761(K) (I)
1261.69441261.6502352973061(K) (N)
1271.57831271.6240-362Met-ox 1111(-)MAKIAQGAMYR(G)
1475.82801475.7593471001131(K) (C)
1532.83681532.7531551231350(R) (D)
1548.80071548.7481341Met-ox 1231350(R)TNEQMHQLVAAYK(D)
2041.07632041.07520.523753931(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (116/673AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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