MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B114
Minimum Matches: 3
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 100000 Da) selects 108217 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 12424 entries.
Pre searches select 12424 entries.

Data Set 1 Results

MS-Fit search selects 2057 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/93(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.446e+0046 (6)25.06.519.445.4 22255/6.1MOUSETumor necrosis factor ligand superfamily member 4 (OX40 ligand) (OX40L)
253947 (7)13.07.59.4344.8 78092/9.2HUMANRING finger protein 6
339798 (8)13.08.611.243.3 95479/8.3HUMANSECIS-binding protein 2
438366 (6)43.06.58.8763.6 19290/9.9HUMANZinc finger protein 160 (HZF5)
5358710 (10)15.010.81.4546.2 82268/9.0HUMANZinc finger protein 224
628297 (7)13.07.5-15.950.4 56080/5.7MOUSEBaculoviral IAP repeat-containing protein 4 (Inhibitor of apoptosis protein 3) (X-linked inhibitor of apoptosis protein) (X-linked IAP) (IAP homolog A) (MIAP3) (MIAP-3)
720677 (7)10.07.510.455.0 99916/6.4MOUSEVav-2 protein
820076 (6)9.06.54.5255.1 81277/5.1HUMANVascular cell adhesion protein 1 precursor (V-CAM 1) (CD106 antigen) (INCAM-100)
919456 (6)15.06.5-2.4078.3 45568/8.9HUMANMAP kinase-activated protein kinase 2 (MAPK-activated protein kinase 2) (MAPKAP kinase 2) (MAPKAPK-2)
1018307 (7)12.07.54.8556.6 60067/8.1HUMANKeratin, type II cytoskeletal 6F (Cytokeratin 6F) (CK 6F) (K6F keratin)
114317................x....................x.............................x..x....x...x.............
99496x.................x...x.....................................x........x....x......x...........
19862...x...............x...............x..........x........................xx.........x........x.
43708...........................x.....x.........x...............................x..x.....x........
66727....................x.x.................x..............x.........x.x..x..xxx.................
61712...........x....x..x.......xxx.............x.................................................
13161............x...........x...............x..............xx........................x.........x.
24961..............x......x....x.................................x..........................x..x..
87717...........x.....x........x..................x......................x........x...............
21359...............................x.xx.......xx..x........x.....................................



Detailed Results

1. 6/93 matches (6%).
Acc. #: Species: MOUSE Name: Tumor necrosis factor ligand superfamily member 4 (OX40 ligand) (OX40L)
Index: MW: 22255 Da pI: 6.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
941.4480941.4733-271081150(K) (I)
1271.70701271.671028981070(K) (G)
1707.85911707.8124271221360(R) (R)
1723.85911723.8074301Met-ox 1221360(R)EDHNPISIPMLNDGR(R)
1863.96601863.9136281221371(R) (I)
1945.00661944.94893065811(R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (51/198AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/93 matches (7%).
Acc. #: Species: HUMAN Name: RING finger protein 6
Index: MW: 78092 Da pI: 9.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
874.5283874.5110204444501(R) (S)
982.5299982.5434-142372470(R) (A)
1048.47921048.4812-1.91401480(R) (F)
1650.92001650.91791.35735870(R) (L)
1721.86891721.7843492532680(R) (Q)
1862.87891862.8997-1181961(K) (D)
1962.96421962.9197231031180(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (90/685AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/93 matches (8%).
Acc. #: Species: HUMAN Name: SECIS-binding protein 2
Index: MW: 95479 Da pI: 8.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
888.4703888.4315448150(R) (L)
1008.52151008.51882.66596661(R) (A)
1230.71681230.6959173543631(K) (S)
1507.76491507.75794.65035160(K) (K)
1765.82811765.8509-136086230(R) (I)
1773.89421773.9421-276406541(K) (F)
2041.18472041.1367244434611(K) (K)
2041.18472041.1367244444621(K) (Q)
2584.44292584.3769264704941(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (116/854AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/93 matches (6%).
Acc. #: Species: HUMAN Name: Zinc finger protein 160 (HZF5)
Index: MW: 19290 Da pI: 9.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1090.50011090.5355-321261340(R) (C)
1169.50761169.5196-101351431(K) (G)
1407.73951407.72461157680(K) (R)
1863.96601863.88464470841(R) (A)
1941.01821941.0306-6.429461(K) (K)
2208.17942208.0732481161341(R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 43% (73/169AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/93 matches (10%).
Acc. #: Species: HUMAN Name: Zinc finger protein 224
Index: MW: 82268 Da pI: 9.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1023.44971023.4933-4335420(R) (N)
1048.47921048.4886-9.01Met-ox 5255320(K)FNLDMHQK(V)
1309.67051309.6761-4.32Met-ox 65741(K)IWMMKTAIQR(E)
1608.79571608.78834.62682800(K) (I)
1691.86801691.8804-7.31841961(K) (V)
1707.85911707.84239.86656781(R) (T)
1723.85911723.8372131Met-ox 6656781(R)LNLDMHQRVHMGEK(T)
1835.82901835.8420-7.15896031(R) (G)
1862.87891862.86427.95335471(K) (S)
1863.96601863.8733506456591(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (111/707AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 7/93 matches (7%).
Acc. #: Species: MOUSE Name: Baculoviral IAP repeat-containing protein 4 (Inhibitor of apoptosis protein 3) (X-linked inhibitor of apoptosis protein) (X-linked IAP) (IAP homolog A) (MIAP3) (MIAP-3)
Index: MW: 56080 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
923.3809923.4070-282392470(R) (N)
941.4480941.4706-242162221(R) (H)
1008.52151008.5366-1511190(R) (D)
1090.50011090.5380-354334410(K) (R)
1127.54451127.53736.32862960(R) (V)
1133.48441133.5340-442482570(R) (N)
1407.73951407.6996282232330(R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (66/496AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 7/93 matches (7%).
Acc. #: Species: MOUSE Name: Vav-2 protein
Index: MW: 99916 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
926.3655926.35996.01861930(K) (R)
1067.57061067.5890-172672741(K) (L)
1309.67051309.7004-237447541(K) (F)
1608.79571608.7613211952070(R) (Y)
1616.94171616.8760416897021(R) (F)
1962.96421962.8696486556700(R) (Q)
2584.44292584.4516-3.416381(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (93/868AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/93 matches (6%).
Acc. #: Species: HUMAN Name: Vascular cell adhesion protein 1 precursor (V-CAM 1) (CD106 antigen) (INCAM-100)
Index: MW: 81277 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
933.4458933.4893-476396470(K) (S)
1045.59171045.5530373493580(R) (V)
1045.59171045.5682224274350(K) (L)
1081.53971081.5530-127277360(K) (S)
1650.92001650.91075.71041171(K) (D)
2267.23802267.22874.13033220(K) (I)
2395.37482395.3237213023221(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (73/739AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/93 matches (6%).
Acc. #: Species: HUMAN Name: MAP kinase-activated protein kinase 2 (MAPK-activated protein kinase 2) (MAPKAP kinase 2) (MAPKAPK-2)
Index: MW: 45568 Da pI: 8.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
923.3809923.4223-451541610(R) (E)
968.5400968.4954461041100(R) (A)
1081.53971081.5577-171111190(R) (I)
1475.80131475.8698-463313431(K) (E)
1711.83151711.7961211541681(R) (S)
1922.07601922.0248271691850(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (61/400AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/93 matches (7%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 6F (Cytokeratin 6F) (CK 6F) (K6F keratin)
Index: MW: 60067 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1153.58861153.5563284474550(K) (L)
1169.50761169.5512-371Met-ox 4474550(K)EYQELMNVK(L)
1179.63421179.6010283603690(K) (H)
1350.71841350.6766313393491(K) (A)
1350.71841350.7269-6.32612721(R) (D)
1407.73951407.7120202882990(K) (A)
1507.76491507.8194-364444551(R) (L)
1608.79571608.7764121151(-) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (72/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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