MS-Fit Search Results

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B115
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 3621 entries (results displayed for top 10 matches).

Results Summary

	MOWSE Score	#/112(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	4.830e+006	12 (10)	37.0	10.7	70.7	12.5	28994/6.8	HUMAN	Endoplasmic reticulum protein ERp29 precursor (ERp31) (ERp28)
2	4.367e+006	16 (14)	25.0	14.3	35.0	108	82655/6.4	HUMAN	Vesicular-fusion protein NSF (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein)
3	1.488e+006	11 (9)	24.0	9.8	45.8	55.8	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
4	1.380e+006	16 (14)	14.0	14.3	50.9	79.8	95339/6.4	HUMAN	Elongation factor 2 (EF-2)
5	1.002e+006	9 (8)	18.0	8.0	41.5	62.4	42321/8.2	HUMAN	Hypothetical protein PP2447
6	4.900e+005	12 (10)	19.0	10.7	56.4	91.6	59519/5.1	HUMAN	Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
7	4.022e+005	9 (8)	18.0	8.0	41.2	53.7	42189/8.7	MOUSE	Hypothetical protein PP2447 homolog
8	2.424e+005	11 (9)	26.0	9.8	21.2	86.6	59665/9.1	HUMAN	Serine/threonine-protein kinase PCTAIRE-2
9	2.069e+005	10 (8)	13.0	8.9	41.6	71.9	82724/7.7	HUMAN	Ribosomal protein S6 kinase alpha 1 (S6K-alpha 1) (90 kDa ribosomal protein S6 kinase 1) (p90-RSK 1) (Ribosomal S6 kinase 1) (RSK-1) (pp90RSK1)
10	2.034e+005	15 (13)	13.0	13.4	53.4	75.0	95315/6.4	MOUSE	Elongation factor 2 (EF-2)

101427	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.
16203	.	x	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	x	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	x	.	.
45313	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.
55806	.	x	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	x	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
86008	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.
108237	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.
42012	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.
41703	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	x	.	.	.	x	.	.	.	.	.	.
32637	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	x	x	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
37935	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	.	x	.	.	.	.	.	.	.	.	x	.	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 12/112 matches (10%).
Acc. #: Species: HUMAN Name: Endoplasmic reticulum protein ERp29 precursor (ERp31) (ERp28)
Index: MW: 28994 Da pI: 6.8

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
937.5508	937.4783	77		198	204	0	(K) (I)
1058.5941	1058.5118	78	pyroGlu	183	192	0	(K)QGQDNLSSVK(E)
1065.6548	1065.5733	76		197	204	1	(K) (I)
1134.7265	1134.6523	65		244	253	0	(K) (K)
1247.6428	1247.5584	68		60	69	0	(K) (Q)
1320.7584	1320.6628	72		113	122	0	(K) (D)
1324.8010	1324.7153	65		37	48	0	(K) (V)
1608.8656	1608.7546	69		123	137	0	(R) (V)
1676.9759	1676.8688	64		110	122	1	(K) (D)
1708.9012	1708.7852	68		209	223	0	(K) (I)
1724.8915	1724.7801	65	1Met-ox	209	223	0	(K)ILDQGEDFPASEMTR(I)
1895.0055	1894.8499	82		60	74	1	(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 37% (98/261AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/112 matches (14%).
Acc. #: Species: HUMAN Name: Vesicular-fusion protein NSF (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein)
Index: MW: 82655 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
949.5364 949.4525 88 447 455 0 (R) (H)
973.5380 973.6046 -68 640 648 0 (K) (K)
1013.5924 1013.5566 35 pyroGlu 272 280 1 (R)QIGKMLNAR(E)
1030.6368 1030.5832 52 272 280 1 (R) (E)
1046.6306 1046.5781 50 1Met-ox 272 280 1 (R)QIGKMLNAR(E)
1069.6319 1069.5431 83 226 233 1 (K) (A)
1073.6334 1073.6319 1.4 692 701 1 (R) (K)
1201.6531 1201.7170 -53 404 413 0 (R) (M)
1247.6428 1247.6193 19 151 161 0 (K) (G)
1263.7236 1263.6142 87 1Met-ox 151 161 0 (K)DIESMDPSILK(G)
1363.7818 1363.6606 89 305 315 1 (K) (L)
1630.8570 1630.9168 -37 534 549 0 (R) (T)
1697.9551 1697.8243 77 567 581 1 (K) (C)
1994.0948 1993.9211 87 11 27 0 (R) (D)
2284.2936 2284.1356 69 pyroGlu 1Met-ox 336 357 1 (K)QRGSMAGSTGVHDTVVNQLLSK(I)
2284.2936 2284.1865 47 68 87 1 (R) (A)
2369.1893 2369.2651 -32 315 335 1 (R) (Q)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
949.5364	949.4525	88		447	455	0	(R) (H)
973.5380	973.6046	-68		640	648	0	(K) (K)
1013.5924	1013.5566	35	pyroGlu	272	280	1	(R)QIGKMLNAR(E)
1030.6368	1030.5832	52		272	280	1	(R) (E)
1046.6306	1046.5781	50	1Met-ox	272	280	1	(R)QIGKMLNAR(E)
1069.6319	1069.5431	83		226	233	1	(K) (A)
1073.6334	1073.6319	1.4		692	701	1	(R) (K)
1201.6531	1201.7170	-53		404	413	0	(R) (M)
1247.6428	1247.6193	19		151	161	0	(K) (G)
1263.7236	1263.6142	87	1Met-ox	151	161	0	(K)DIESMDPSILK(G)
1363.7818	1363.6606	89		305	315	1	(K) (L)
1630.8570	1630.9168	-37		534	549	0	(R) (T)
1697.9551	1697.8243	77		567	581	1	(K) (C)
1994.0948	1993.9211	87		11	27	0	(R) (D)
2284.2936	2284.1356	69	pyroGlu 1Met-ox	336	357	1	(K)QRGSMAGSTGVHDTVVNQLLSK(I)
2284.2936	2284.1865	47		68	87	1	(R) (A)
2369.1893	2369.2651	-32		315	335	1	(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (187/744AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/112 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
973.5380 973.5318 6.3 396 403 0 (K) (V)
1073.6334 1073.5955 35 408 416 1 (R) (K)
1179.6642 1179.6010 54 377 386 0 (K) (H)
1300.6531 1300.5302 94 258 267 0 (K) (N)
1302.7459 1302.7018 34 393 403 1 (R) (V)
1302.7459 1302.7157 23 344 355 0 (R) (A)
1383.7868 1383.6909 69 186 197 0 (K) (V)
1469.8306 1469.8440 -9.1 396 407 1 (K) (L)
1475.8519 1475.7494 69 212 223 0 (K) (T)
1475.8519 1475.7858 45 200 211 0 (R) (W)
1688.0617 1687.9707 54 66 82 1 (R) (G)
1994.0948 1993.9772 59 224 239 0 (R) (R)
2384.0983 2383.9524 61 519 549 0 (R) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
973.5380	973.5318	6.3	396	403	0	(K) (V)
1073.6334	1073.5955	35	408	416	1	(R) (K)
1179.6642	1179.6010	54	377	386	0	(K) (H)
1300.6531	1300.5302	94	258	267	0	(K) (N)
1302.7459	1302.7018	34	393	403	1	(R) (V)
1302.7459	1302.7157	23	344	355	0	(R) (A)
1383.7868	1383.6909	69	186	197	0	(K) (V)
1469.8306	1469.8440	-9.1	396	407	1	(K) (L)
1475.8519	1475.7494	69	212	223	0	(K) (T)
1475.8519	1475.7858	45	200	211	0	(R) (W)
1688.0617	1687.9707	54	66	82	1	(R) (G)
1994.0948	1993.9772	59	224	239	0	(R) (R)
2384.0983	2383.9524	61	519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (156/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 16/112 matches (14%).
Acc. #: Species: HUMAN Name: Elongation factor 2 (EF-2)
Index: MW: 95339 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
949.5364 949.4478 93 323 330 1 (K) (E)
973.5380 973.5008 38 408 415 1 (K) (V)
1013.5924 1013.5016 90 240 249 0 (K) (A)
1039.6126 1039.5320 78 392 400 0 (K) (M)
1055.5987 1055.5269 68 1Met-ox 392 400 0 (K)GPLMMYISK(M)
1073.6334 1073.6215 11 151 159 0 (R) (M)
1107.6366 1107.6414 -4.3 416 426 0 (R) (V)
1305.7667 1305.6473 91 62 71 1 (K) (S)
1307.7523 1307.6530 76 21 32 0 (R) (S)
1308.7581 1308.6588 76 677 688 0 (K) (E)
1323.7735 1323.6479 95 1Met-ox 21 32 0 (R)NMSVIAHVDHGK(S)
1475.8519 1475.7901 42 151 162 1 (R) (A)
1491.8398 1491.7850 37 1Met-ox 151 162 1 (R)IKPVLMMNKMDR(A)
1608.8656 1608.7791 54 581 594 0 (R) (S)
1707.8863 1707.8740 7.2 AcetN 1Met-ox 1 15 1 (-)VNFTVDQIRAIMDK(K)
1724.8915 1724.9555 -37 pyroGlu 145 159 1 (R)QAIAERIKPVLMMNK(M)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
949.5364	949.4478	93		323	330	1	(K) (E)
973.5380	973.5008	38		408	415	1	(K) (V)
1013.5924	1013.5016	90		240	249	0	(K) (A)
1039.6126	1039.5320	78		392	400	0	(K) (M)
1055.5987	1055.5269	68	1Met-ox	392	400	0	(K)GPLMMYISK(M)
1073.6334	1073.6215	11		151	159	0	(R) (M)
1107.6366	1107.6414	-4.3		416	426	0	(R) (V)
1305.7667	1305.6473	91		62	71	1	(K) (S)
1307.7523	1307.6530	76		21	32	0	(R) (S)
1308.7581	1308.6588	76		677	688	0	(K) (E)
1323.7735	1323.6479	95	1Met-ox	21	32	0	(R)NMSVIAHVDHGK(S)
1475.8519	1475.7901	42		151	162	1	(R) (A)
1491.8398	1491.7850	37	1Met-ox	151	162	1	(R)IKPVLMMNKMDR(A)
1608.8656	1608.7791	54		581	594	0	(R) (S)
1707.8863	1707.8740	7.2	AcetN 1Met-ox	1	15	1	(-)VNFTVDQIRAIMDK(K)
1724.8915	1724.9555	-37	pyroGlu	145	159	1	(R)QAIAERIKPVLMMNK(M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (127/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/112 matches (8%).
Acc. #: Species: HUMAN Name: Hypothetical protein PP2447
Index: MW: 42321 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1036.6206 1036.6128 7.5 55 62 1 (R) (T)
1046.6306 1046.5370 89 125 133 0 (R) (L)
1134.7265 1134.6312 84 200 209 0 (R) (V)
1275.7072 1275.6545 41 63 74 0 (R) (V)
1676.9759 1676.8902 51 140 154 0 (R) (V)
1707.8863 1707.8484 22 pyroGlu 3Met-ox 140 154 0 (R)QNGLMSGLMQMLLLK(V)
1708.9012 1708.8800 12 2Met-ox 140 154 0 (R)QNGLMSGLMQMLLLK(V)
1724.8915 1724.8749 9.6 3Met-ox 140 154 0 (R)QNGLMSGLMQMLLLK(V)
1852.0286 1851.9256 56 336 351 0 (K) (R)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1036.6206	1036.6128	7.5		55	62	1	(R) (T)
1046.6306	1046.5370	89		125	133	0	(R) (L)
1134.7265	1134.6312	84		200	209	0	(R) (V)
1275.7072	1275.6545	41		63	74	0	(R) (V)
1676.9759	1676.8902	51		140	154	0	(R) (V)
1707.8863	1707.8484	22	pyroGlu 3Met-ox	140	154	0	(R)QNGLMSGLMQMLLLK(V)
1708.9012	1708.8800	12	2Met-ox	140	154	0	(R)QNGLMSGLMQMLLLK(V)
1724.8915	1724.8749	9.6	3Met-ox	140	154	0	(R)QNGLMSGLMQMLLLK(V)
1852.0286	1851.9256	56		336	351	0	(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (70/376AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 12/112 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
995.4471 995.5202 -73 178 184 1 (K) (H)
1090.6237 1090.5315 85 148 156 0 (K) (L)
1109.5989 1109.4904 98 335 343 0 (K) (S)
1165.6989 1165.5853 97 442 450 0 (R) (S)
1201.6531 1201.6177 29 246 256 0 (R) (R)
1234.7587 1234.6796 64 236 245 1 (R) (Q)
1300.6531 1300.5956 44 286 295 1 (K) (N)
1365.7493 1365.6399 80 323 333 0 (R) (K)
1434.8384 1434.7705 47 440 450 1 (K) (S)
1493.8349 1493.7348 67 323 334 1 (R) (D)
1707.8863 1707.7727 67 41 59 0 (K) (G)
2097.1309 2096.9810 71 423 439 0 (R) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
995.4471	995.5202	-73	178	184	1	(K) (H)
1090.6237	1090.5315	85	148	156	0	(K) (L)
1109.5989	1109.4904	98	335	343	0	(K) (S)
1165.6989	1165.5853	97	442	450	0	(R) (S)
1201.6531	1201.6177	29	246	256	0	(R) (R)
1234.7587	1234.6796	64	236	245	1	(R) (Q)
1300.6531	1300.5956	44	286	295	1	(K) (N)
1365.7493	1365.6399	80	323	333	0	(R) (K)
1434.8384	1434.7705	47	440	450	1	(K) (S)
1493.8349	1493.7348	67	323	334	1	(R) (D)
1707.8863	1707.7727	67	41	59	0	(K) (G)
2097.1309	2096.9810	71	423	439	0	(R) (I)

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The matched peptides cover 19% (115/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/112 matches (8%).
Acc. #: Species: MOUSE Name: Hypothetical protein PP2447 homolog
Index: MW: 42189 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1033.6223 1033.5464 73 116 123 1 (K) (E)
1037.6186 1037.5968 21 54 61 1 (R) (T)
1134.7265 1134.6312 84 199 208 0 (R) (V)
1275.7072 1275.6545 41 62 73 0 (R) (V)
1676.9759 1676.8902 51 139 153 0 (R) (V)
1707.8863 1707.8484 22 pyroGlu 3Met-ox 139 153 0 (R)QNGLMSGLMQMLLLK(V)
1708.9012 1708.8800 12 2Met-ox 139 153 0 (R)QNGLMSGLMQMLLLK(V)
1724.8915 1724.8749 9.6 3Met-ox 139 153 0 (R)QNGLMSGLMQMLLLK(V)
1852.0286 1851.9256 56 335 350 0 (K) (R)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1033.6223	1033.5464	73		116	123	1	(K) (E)
1037.6186	1037.5968	21		54	61	1	(R) (T)
1134.7265	1134.6312	84		199	208	0	(R) (V)
1275.7072	1275.6545	41		62	73	0	(R) (V)
1676.9759	1676.8902	51		139	153	0	(R) (V)
1707.8863	1707.8484	22	pyroGlu 3Met-ox	139	153	0	(R)QNGLMSGLMQMLLLK(V)
1708.9012	1708.8800	12	2Met-ox	139	153	0	(R)QNGLMSGLMQMLLLK(V)
1724.8915	1724.8749	9.6	3Met-ox	139	153	0	(R)QNGLMSGLMQMLLLK(V)
1852.0286	1851.9256	56		335	350	0	(K) (R)

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The matched peptides cover 18% (69/376AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 11/112 matches (9%).
Acc. #: Species: HUMAN Name: Serine/threonine-protein kinase PCTAIRE-2
Index: MW: 59665 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
949.5364 949.4665 74 136 143 0 (R) (R)
1008.5413 1008.5366 4.7 179 188 0 (R) (M)
1046.6306 1046.5318 94 300 307 1 (R) (K)
1065.6548 1065.6461 8.2 292 299 0 (K) (G)
1201.6531 1201.6105 35 198 208 0 (K) (G)
1434.8384 1434.7858 37 430 441 0 (K) (L)
1566.8433 1566.7739 44 462 474 1 (R) (S)
1712.8444 1712.9084 -37 247 261 0 (K) (S)
1852.0286 1852.0544 -14 313 328 1 (R) (L)
1895.0055 1894.9445 32 222 237 1 (K) (E)
2051.0719 2051.1653 -46 475 493 1 (R) (E)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
949.5364	949.4665	74	136	143	0	(R) (R)
1008.5413	1008.5366	4.7	179	188	0	(R) (M)
1046.6306	1046.5318	94	300	307	1	(R) (K)
1065.6548	1065.6461	8.2	292	299	0	(K) (G)
1201.6531	1201.6105	35	198	208	0	(K) (G)
1434.8384	1434.7858	37	430	441	0	(K) (L)
1566.8433	1566.7739	44	462	474	1	(R) (S)
1712.8444	1712.9084	-37	247	261	0	(K) (S)
1852.0286	1852.0544	-14	313	328	1	(R) (L)
1895.0055	1894.9445	32	222	237	1	(K) (E)
2051.0719	2051.1653	-46	475	493	1	(R) (E)

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The matched peptides cover 26% (136/523AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 10/112 matches (8%).
Acc. #: Species: HUMAN Name: Ribosomal protein S6 kinase alpha 1 (S6K-alpha 1) (90 kDa ribosomal protein S6 kinase 1) (p90-RSK 1) (Ribosomal S6 kinase 1) (RSK-1) (pp90RSK1)
Index: MW: 82724 Da pI: 7.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1156.7003 1156.6002 86 57 66 0 (K) (V)
1165.6989 1165.6985 0.37 137 145 0 (K) (G)
1193.7147 1193.5955 100 127 136 0 (K) (L)
1201.6531 1201.6581 -4.2 511 521 0 (R) (T)
1260.7709 1260.6775 74 484 493 0 (K) (G)
1283.7551 1283.7112 34 466 476 0 (R) (D)
1346.7989 1346.7221 57 pyroGlu 668 678 0 (K)QVLQHPWVTQK(D)
1363.7818 1363.7486 24 668 678 0 (K) (D)
1469.8306 1469.7964 23 454 465 1 (K) (Y)
1724.8915 1724.8568 20 300 316 1 (R) (R)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1156.7003	1156.6002	86		57	66	0	(K) (V)
1165.6989	1165.6985	0.37		137	145	0	(K) (G)
1193.7147	1193.5955	100		127	136	0	(K) (L)
1201.6531	1201.6581	-4.2		511	521	0	(R) (T)
1260.7709	1260.6775	74		484	493	0	(K) (G)
1283.7551	1283.7112	34		466	476	0	(R) (D)
1346.7989	1346.7221	57	pyroGlu	668	678	0	(K)QVLQHPWVTQK(D)
1363.7818	1363.7486	24		668	678	0	(K) (D)
1469.8306	1469.7964	23		454	465	1	(K) (Y)
1724.8915	1724.8568	20		300	316	1	(R) (R)

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The matched peptides cover 13% (101/735AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 15/112 matches (13%).
Acc. #: Species: MOUSE Name: Elongation factor 2 (EF-2)
Index: MW: 95315 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
949.5364 949.4478 93 323 330 1 (K) (E)
973.5380 973.5008 38 408 415 1 (K) (V)
1039.6126 1039.5320 78 392 400 0 (K) (M)
1055.5987 1055.5269 68 1Met-ox 392 400 0 (K)GPLMMYISK(M)
1073.6334 1073.6215 11 151 159 0 (R) (M)
1305.7667 1305.6473 91 62 71 1 (K) (S)
1307.7523 1307.6530 76 21 32 0 (R) (S)
1308.7581 1308.6588 76 677 688 0 (K) (E)
1323.7735 1323.6479 95 1Met-ox 21 32 0 (R)NMSVIAHVDHGK(S)
1434.8384 1434.7341 73 236 249 1 (K) (A)
1475.8519 1475.7901 42 151 162 1 (R) (A)
1491.8398 1491.7850 37 1Met-ox 151 162 1 (R)IKPVLMMNKMDR(A)
1608.8656 1608.7791 54 581 594 0 (R) (S)
1707.8863 1707.8740 7.2 AcetN 1Met-ox 1 15 1 (-)VNFTVDQIRAIMDK(K)
1724.8915 1724.9555 -37 pyroGlu 145 159 1 (R)QAIAERIKPVLMMNK(M)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
949.5364	949.4478	93		323	330	1	(K) (E)
973.5380	973.5008	38		408	415	1	(K) (V)
1039.6126	1039.5320	78		392	400	0	(K) (M)
1055.5987	1055.5269	68	1Met-ox	392	400	0	(K)GPLMMYISK(M)
1073.6334	1073.6215	11		151	159	0	(R) (M)
1305.7667	1305.6473	91		62	71	1	(K) (S)
1307.7523	1307.6530	76		21	32	0	(R) (S)
1308.7581	1308.6588	76		677	688	0	(K) (E)
1323.7735	1323.6479	95	1Met-ox	21	32	0	(R)NMSVIAHVDHGK(S)
1434.8384	1434.7341	73		236	249	1	(K) (A)
1475.8519	1475.7901	42		151	162	1	(R) (A)
1491.8398	1491.7850	37	1Met-ox	151	162	1	(R)IKPVLMMNKMDR(A)
1608.8656	1608.7791	54		581	594	0	(R) (S)
1707.8863	1707.8740	7.2	AcetN 1Met-ox	1	15	1	(-)VNFTVDQIRAIMDK(K)
1724.8915	1724.9555	-37	pyroGlu	145	159	1	(R)QAIAERIKPVLMMNK(M)

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The matched peptides cover 13% (120/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):