MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B116
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 100000 Da) selects 108217 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 12424 entries.
Pre searches select 12424 entries.

Data Set 1 Results

MS-Fit search selects 327 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/60(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.313e+0058 (13)18.013.3-38.859.0 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
234377 (11)10.011.7-43.375.1 96821/5.5HUMANTuftelin-interacting protein 11 (HSPC006)
3143510 (16)14.016.7-37.0105 86984/6.5HUMANCullin homolog 2 (CUL-2)
412868 (13)16.013.3-46.282.3 66472/6.7MOUSERING finger protein 12 (LIM domain interacting RING finger protein) (RING finger LIM domain-binding protein) (R-LIM)
512278 (13)11.013.3-62.070.0 82437/9.1HUMANZinc finger protein 225
45849.......................x....x..x...x..x...x...x.........x...
27380............................xx.x.....x....x..x.........x....
52225..........x...........xxx..x.....xx.x................x..x...
82136.x.....x...x.....................x...........x.xx...x.......
75556..x.....................x..xx..x.......x....x....x..........



Detailed Results

1. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1179.51071179.6010-773773860(K) (H)
1300.49201300.5302-292582670(K) (N)
1316.54231316.5251131Met-ox 2582670(K)NMQDMVEDYR(N)
1357.63911357.6963-424444550(K) (E)
1383.59141383.6909-721861970(K) (V)
1475.69281475.7494-382122230(K) (T)
1475.69281475.7858-632002110(R) (W)
1687.91201687.9707-3566821(R) (G)
2383.93772383.9524-6.25195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (116/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Tuftelin-interacting protein 11 (HSPC006)
Index: MW: 96821 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1300.49201300.6095-904444540(K) (W)
1307.57581307.5975-173763850(K) (R)
1316.54231316.5581-127427510(K) (R)
1377.59831377.7200-887617740(R) (D)
1475.69281475.7873-644804900(R) (N)
1475.69281475.7892-652622741(K) (E)
1614.78381614.78380.0214444561(K) (S)
2283.18432283.2059-9.47757941(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (91/837AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: Cullin homolog 2 (CUL-2)
Index: MW: 86984 Da pI: 6.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1052.44051052.5450-991Met-ox 7117190(R)FNPSISMIK(K)
1173.48881173.6057-1004294371(K) (M)
1179.51071179.4952134054140(K) (L)
1234.54871234.53749.16606690(K) (S)
1293.54021293.5244121Met-ox 971060(K)GADYMDCLYR(Y)
1322.48151322.5898-825986080(K) (E)
1339.55231339.6503-732923020(K) (A)
1371.69231371.6401382Met-ox 2923020(K)NDMANMYVLLR(A)
2193.97742194.0306-242Met-ox 4424601(K)RLIHGLSMSMDSEEAMINK(L)
2383.93772384.0903-642Met-ox 4764951(R)MYTDMSVSADLNNKFNNFIK(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (109/745AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/60 matches (13%).
Acc. #: Species: MOUSE Name: RING finger protein 12 (LIM domain interacting RING finger protein) (RING finger LIM domain-binding protein) (R-LIM)
Index: MW: 66472 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1007.39631007.4546-581271350(R) (F)
1036.38111036.4846-1001Met-ox 20271(R)SQMDRLDR(E)
1055.38201055.3951-121Met-ox 190(-)MENSDSNDK(G)
1322.48151322.5977-8872830(K) (A)
1614.78381614.8274-271Met-ox 2272401(R)SMSPLQPTSEIPRR(A)
1690.73991690.73045.65455580(K) (L)
1690.73991690.7455-3.3pyroGlu 1741890(R)QMENSASESASARPSR(A)
1707.71181707.7720-351741890(R) (A)
1940.88871941.0783-982602761(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (99/600AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Zinc finger protein 225
Index: MW: 82437 Da pI: 9.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1009.39641009.4777-804524590(R) (E)
1234.54871234.6479-801932021(R) (L)
1293.54021293.6479-8355641(R) (F)
1300.49201300.5818-6965740(K) (E)
1300.49201300.6030-853183281(R) (S)
1316.54231316.5768-261Met-ox 65740(K)FWMMETATQR(E)
1425.55581425.6619-743133231(R) (S)
1566.66021566.7811-773293421(K) (R)
1763.78001763.7481184024151(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (82/706AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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