MS-Fit Search Results



.

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B119
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 3596 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/132(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.699e+00815 (11)45.011.411.953.3 29547/6.0MOUSEProteasome subunit alpha type 1 (Proteasome component C2) (Macropain subunit C2) (Multicatalytic endopeptidase complex subunit C2) (Proteasome nu chain)
24.698e+00816 (12)51.012.113.152.4 29556/6.1HUMANProteasome subunit alpha type 1 (Proteasome component C2) (Macropain subunit C2) (Multicatalytic endopeptidase complex subunit C2) (Proteasome nu chain) (30 kDa prosomal protein) (PROS-30)
32.280e+00618 (13)21.013.6-12.289.3 89679/8.2HUMANCullin homolog 1 (CUL-1)
42.280e+00618 (13)21.013.6-12.289.3 89692/8.2MOUSECullin homolog 1 (CUL-1)
51.979e+00617 (12)28.012.95.7575.4 74210/6.5MOUSELamin A
61.049e+00610 (7)21.07.618.555.6 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
77.169e+00515 (11)23.011.49.3965.1 82437/9.1HUMANZinc finger protein 225
85.649e+00512 (9)23.09.1-9.2664.7 59621/5.4HUMANT-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
93.042e+00514 (10)25.010.619.362.2 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
102.640e+00513 (9)17.09.84.8787.5 93559/7.8HUMANAxin 2 (Axis inhibition protein 2) (Conductin) (Axin-like protein) (Axil)
113098................x......x............x.....x...................x..x...........x.........x.x....xx.....x.....x...............x..x.....
20667................x......x............x.....x...................x..x...........x.........x.x....xx.....x.....x..........x....x..x.....
112914.......xx.x.....x...........x.............x...x..............x..........xx.............x..x........xx.x.............x.........x..x..
91051.......xx.x.....x...........x.............x...x..............x..........xx.............x..x........xx.x.............x.........x..x..
14097...................x...........x....................xx...x...........x................x....x....x..x..x..x.x....x....x...x......x...
45313...........................x...................x..x....................x.........x..x........x..x.........................x.....x...
74665.....x........x......x.............x...x........x......x.x..........x..x.....x...x..x.............xx................................
61980...................x......................x....x...........x................x.x...x........xx..................x....x...........x...
97443..............x......x..............x......................x.xx..........xx.......xx.......x..................x.....x............x..
14223............x........................x................x......x..x..................x..x....xx....xx...........x.......x.............



Detailed Results

1. 15/132 matches (11%).
Acc. #: Species: MOUSE Name: Proteasome subunit alpha type 1 (Proteasome component C2) (Macropain subunit C2) (Multicatalytic endopeptidase complex subunit C2) (Proteasome nu chain)
Index: MW: 29547 Da pI: 6.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
904.3852904.38351.9pyroGlu 90960(R)QECLDSR(F)
921.4336921.41002690960(R) (F)
967.4821967.4858-3.883890(R) (Q)
983.4838983.48073.21Met-ox 83890(R)LLCNFMR(Q)
1082.57431082.55941452610(R) (K)
1107.62561107.7002-6742511(K) (A)
1234.70281234.6326571581691(R) (T)
1332.77911332.742827971070(R) (L)
1345.69831345.68519.81972080(R) (N)
1431.75751431.73061919300(R) (Q)
1447.75141447.7255181Met-ox 19300(R)IHQIEYAMEAVK(Q)
1685.91511685.90744.61942081(R) (N)
1778.86631778.8098324180(R) (I)
2086.20022086.14092863820(K) (L)
2214.25972214.23591162821(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 45% (120/263AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/132 matches (12%).
Acc. #: Species: HUMAN Name: Proteasome subunit alpha type 1 (Proteasome component C2) (Macropain subunit C2) (Multicatalytic endopeptidase complex subunit C2) (Proteasome nu chain) (30 kDa prosomal protein) (PROS-30)
Index: MW: 29556 Da pI: 6.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
904.3852904.38351.9pyroGlu 90960(R)QECLDSR(F)
921.4336921.41002690960(R) (F)
967.4821967.4858-3.883890(R) (Q)
983.4838983.48073.21Met-ox 83890(R)LLCNFMR(Q)
1082.57431082.55941452610(R) (K)
1107.62561107.7002-6742511(K) (A)
1234.70281234.6326571581691(R) (T)
1332.77911332.742827971070(R) (L)
1345.69831345.68519.81972080(R) (N)
1431.75751431.73061919300(R) (Q)
1447.75141447.7255181Met-ox 19300(R)IHQIEYAMEAVK(Q)
1685.91511685.90744.61942081(R) (N)
1778.86631778.8098324180(R) (I)
1926.89991926.8400312Met-ox 1751890(R)HMSEFMECNLNELVK(H)
2086.20022086.14092863820(K) (L)
2214.25972214.23591162821(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 51% (135/263AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 18/132 matches (13%).
Acc. #: Species: HUMAN Name: Cullin homolog 1 (CUL-1)
Index: MW: 89679 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
872.4574872.5205-721781850(K) (L)
878.4572878.4987-472222290(K) (E)
882.4515882.45070.843223280(R) (I)
904.3852904.4488-701921990(R) (L)
935.3579935.4005-461471531(R) (K)
983.4838983.443441pyroGlu 6947010(K)QEQETTHK(N)
996.5849996.6570-727097170(K) (I)
1081.55791081.5352215885960(K) (N)
1187.43191187.5043-61pyroGlu 4945030(K)QACGFEYTSK(L)
1189.58641189.5925-5.21901991(K) (L)
1332.77911332.6986603653750(K) (K)
1348.75591348.6935461Met-ox 3653750(K)MYVQTVLDVHK(K)
1566.78171566.76987.63163281(K) (I)
1664.87091664.86483.775900(K) (R)
1707.81521707.8991-497527641(K) (V)
1851.97681851.96397.01Met-ox 3223371(R)MYNLVSRIQDGLGELK(K)
2214.25972214.1633445795961(K) (N)
2705.20682705.2816-281Met-ox 3774011(K)YNALVMSAFNNDAGFVAALDKACGR(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (165/776AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 18/132 matches (13%).
Acc. #: Species: MOUSE Name: Cullin homolog 1 (CUL-1)
Index: MW: 89692 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
872.4574872.5205-721781850(K) (L)
878.4572878.4987-472222290(K) (E)
882.4515882.45070.843223280(R) (I)
904.3852904.4488-701921990(R) (L)
935.3579935.4005-461471531(R) (K)
983.4838983.443441pyroGlu 6947010(K)QEQETTHK(N)
996.5849996.6570-727097170(K) (I)
1081.55791081.5352215885960(K) (N)
1187.43191187.5043-61pyroGlu 4945030(K)QACGFEYTSK(L)
1189.58641189.5925-5.21901991(K) (L)
1332.77911332.6986603653750(K) (K)
1348.75591348.6935461Met-ox 3653750(K)MYVQTVLDVHK(K)
1566.78171566.76987.63163281(K) (I)
1664.87091664.86483.775900(K) (R)
1707.81521707.8991-497527641(K) (V)
1851.97681851.96397.01Met-ox 3223371(R)MYNLVSRIQDGLGELK(K)
2214.25972214.1633445795961(K) (N)
2705.20682705.2816-281Met-ox 3774011(K)YNALVMSAFNNDAGFVAALDKACGR(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (165/776AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 17/132 matches (12%).
Acc. #: Species: MOUSE Name: Lamin A
Index: MW: 74210 Da pI: 6.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
908.4904908.4365592022080(K) (N)
951.4633951.4648-1.62342401(K) (L)
1023.57941023.5111672092160(K) (E)
1031.43601031.4832-46pyroGlu 1Met-ox 1821890(K)QLQDEMLR(R)
1043.53351043.5485-141241330(K) (L)
1131.49071131.5282-3333410(K) (L)
1320.61831320.6409-174284391(R) (V)
1350.71161350.6225665165270(K) (T)
1475.79851475.7681211Met-ox 3673781(K)LALDMEIHAYRK(L)
1566.78171566.7512196296450(R) (S)
1707.81521707.80505.94214351(K) (T)
1761.90411761.827044pyroGlu 4724861(R)QNGDDPLMTYRFPPK(F)
1761.90411761.9234-1163781(R) (A)
1778.86631778.85367.14724861(R) (F)
1778.86631778.8925-1573891(R) (K)
1794.85801794.84855.31Met-ox 4724861(R)QNGDDPLMTYRFPPK(F)
1912.87851912.9292-272022161(K) (E)
1963.95601963.8681455695861(R) (T)
2384.02842384.1846-662412601(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (189/665AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 10/132 matches (7%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
932.4855932.5053-214104171(R) (Q)
999.4922999.4457472902980(K) (V)
999.4922999.4972-5.03873951(R) (I)
1016.45821016.5013-423563640(K) (S)
1179.63531179.6010293773860(K) (H)
1300.58571300.5302432582670(K) (N)
1316.57241316.5251361Met-ox 2582670(K)NMQDMVEDYR(N)
1383.72541383.6909251861970(K) (V)
1475.79851475.7494332122230(K) (T)
1475.79851475.78588.62002110(R) (W)
1994.05211993.9772382242390(R) (R)
2384.02842383.9524325195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (138/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 15/132 matches (11%).
Acc. #: Species: HUMAN Name: Zinc finger protein 225
Index: MW: 82437 Da pI: 9.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
869.4712869.4845-155805870(R) (R)
897.4451897.4187293063120(R) (E)
897.4451897.4253223763821(K) (W)
913.3761913.4137-411Met-ox 3063120(R)HSMVHMR(E)
966.4306966.4137174864921(K) (F)
966.4306966.4137175145201(K) (F)
966.4306966.4137173463521(K) (F)
966.4306966.4137175986041(K) (F)
972.4301972.4539-244084150(K) (Y)
1005.43871005.5005-623934010(R) (C)
1037.54061037.50903135420(R) (N)
1043.53351043.52745.84414480(R) (V)
1121.48881121.4356471751830(K) (S)
1179.63531179.6625-23AcetN 1111(-)TTLKEAVTFK(D)
1234.70281234.6479441932021(R) (L)
1300.58571300.58182.965740(K) (E)
1300.58571300.6030-133183281(R) (S)
1316.57241316.5768-3.31Met-ox 65740(K)FWMMETATQR(E)
1497.79461497.6830754524631(R) (S)
1497.79461497.7167522Met-ox 3013121(R)ANLNRHSMVHMR(E)
1566.78171566.78110.393293421(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (165/706AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 12/132 matches (9%).
Acc. #: Species: HUMAN Name: T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Index: MW: 59621 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
908.4904908.5028-143083140(K) (L)
983.4838983.5348-5255620(K) (L)
999.4922999.5297-381Met-ox 55620(K)MVINHLEK(L)
1065.51341065.5217-7.73914000(R) (V)
1232.62741232.6098142973070(K) (Y)
1278.67331278.6846-8.94404501(K) (A)
1307.71101307.63845621310(K) (N)
1350.71161350.7568-333083181(K) (W)
1354.75281354.7582-4.04084210(R) (Q)
1793.87961793.9319-291561711(R) (Q)
1851.97681851.90234038541(K) (M)
2384.02842384.1114-352612811(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (130/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 14/132 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
897.4451897.4140352332390(R) (I)
913.3761913.4089-361Met-ox 2332390(R)MTLDDFR(I)
967.4821967.4525311841900(K) (K)
1065.51341065.4999131541620(K) (L)
1081.55791081.4948581Met-ox 1541620(K)STMQELNSR(L)
1081.55791081.51394134451(R) (F)
1082.57431082.4941741Met-ox 2422490(K)FEMEQNLR(Q)
1189.58641189.57757.5pyroGlu 1Met-ox 2612700(R)QVLDNLTMEK(S)
1206.57021206.6040-281Met-ox 2612700(R)QVLDNLTMEK(S)
1206.57021206.6152-373173261(K) (Q)
1307.71101307.6782252402491(R) (Q)
1315.72741315.6858322122230(K) (T)
1350.71161350.6878184724861(K) (G)
1791.79001791.7283344905120(R) (G)
1851.97681851.9275273213351(K) (N)
2705.20682705.16171763940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (156/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 13/132 matches (9%).
Acc. #: Species: HUMAN Name: Axin 2 (Axis inhibition protein 2) (Conductin) (Axin-like protein) (Axil)
Index: MW: 93559 Da pI: 7.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
886.4678886.4569123363421(R) (A)
968.5292968.5781-507047120(R) (Q)
1036.51321036.4668457157220(R) (D)
1081.55791081.6006-395855950(K) (E)
1105.50801105.5489-374054140(R) (E)
1315.72741315.6606513954041(R) (E)
1320.61831320.6265-6.27137221(K) (D)
1350.71161350.7130-1.05966100(R) (E)
1354.75281354.6517756606710(R) (T)
1493.75321493.6695565615740(K) (G)
1497.79461497.7661193833941(K) (L)
1791.79001791.9030-6381960(K) (T)
1926.89991926.89413.0AcetN 1Met-ox 1180(-)SSAMLVTCLPDPSSSFR(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (144/843AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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