MS-Fit Search Results

Parameters

Database searched: SwissProt.10.07.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B129
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 114561
Molecular weight search (1000 - 100000 Da) selects 108217 entries.
Full pI range: 114561 entries.
Species search ( HUMAN MOUSE ) selects 14035 entries.
Combined molecular weight, pI and species searches select 12424 entries.
Pre searches select 12424 entries.

Data Set 1 Results

MS-Fit search selects 2711 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/104(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	2.025e+008	15 (14)	30.0	14.4	-55.3	53.4	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
2	1.040e+006	15 (14)	28.0	14.4	-36.9	91.2	68935/6.7	HUMAN	Kinesin light chain 2 (KLC 2)
3	4.093e+005	17 (16)	45.0	16.3	-50.2	36.1	29717/6.6	HUMAN	LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
4	2.814e+005	9 (8)	22.0	8.7	-40.6	98.7	53734/9.1	HUMAN	HYPOTHETICAL PROTEIN DJ1198H6.1
5	2.176e+005	14 (13)	41.0	13.5	-48.1	50.9	29995/6.6	MOUSE	LIM AND SH3 DOMAIN PROTEIN 1 (LASP-1) (MLN 50)

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88162	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	x	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x
58451	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	x	.	.	.	.	x	.	.	.	.	.	.	x	.	.	x	.	.	.	x	x	.	x	.	.	.	.	x	.	.	x	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
103284	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.
65461	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	x	.	.	.	x	.	.	.	.	.	.	x	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 15/104 matches (14%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1092.5051	1092.5034	1.6		603	616	0	(R) (G)
1179.5316	1179.6010	-59		377	386	0	(K) (H)
1265.5552	1265.6378	-65		278	288	0	(R) (K)
1300.4630	1300.5302	-52		258	267	0	(K) (N)
1302.5846	1302.7018	-90		393	403	1	(R) (V)
1383.5944	1383.6909	-70		186	197	0	(K) (V)
1393.5996	1393.7327	-96		278	289	1	(R) (D)
1475.6797	1475.7494	-47		212	223	0	(K) (T)
1475.6797	1475.7858	-72		200	211	0	(R) (W)
1638.7412	1638.8604	-73		186	199	1	(K) (F)
1657.7020	1657.7934	-55		13	29	0	(R) (R)
1687.8454	1687.9707	-74		66	82	1	(R) (G)
1716.7863	1716.8517	-38		418	432	0	(K) (G)
1993.8822	1993.9772	-48		224	239	0	(R) (R)
2017.8815	2017.8847	-1.6	2Met-ox	252	267	1	(R)LDSELKNMQDMVEDYR(N)
2383.8397	2383.9524	-47		519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (197/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 15/104 matches (14%).
Acc. #: Species: HUMAN Name: Kinesin light chain 2 (KLC 2)
Index: MW: 68935 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
982.3781 982.4677 -91 1 8 0 (-) (E)
1003.4178 1003.5060 -88 111 119 0 (R) (L)
1092.5051 1092.5763 -65 214 222 0 (R) (Q)
1151.6049 1151.5771 24 298 306 1 (K) (R)
1177.5412 1177.5384 2.4 1Met-ox 507 518 1 (R)SSRDMAGGAGPR(S)
1183.5869 1183.5629 20 606 616 0 (R) (R)
1185.5303 1185.6380 -91 102 110 0 (R) (E)
1202.5377 1202.6381 -84 443 453 0 (K) (S)
1360.5994 1360.6596 -44 123 134 0 (R) (Q)
1377.5734 1377.6439 -51 340 349 1 (K) (A)
1443.7085 1443.7120 -2.4 379 390 1 (K) (E)
1588.7634 1588.8434 -50 9 22 1 (R) (A)
1678.8185 1678.7502 41 426 439 1 (R) (A)
1716.7863 1716.8040 -10 589 605 0 (K) (T)
2705.0528 2705.2266 -64 1Met-ox 400 422 1 (K)EFGSVNGDNKPIWMHAEEREESK(D)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
982.3781	982.4677	-91		1	8	0	(-) (E)
1003.4178	1003.5060	-88		111	119	0	(R) (L)
1092.5051	1092.5763	-65		214	222	0	(R) (Q)
1151.6049	1151.5771	24		298	306	1	(K) (R)
1177.5412	1177.5384	2.4	1Met-ox	507	518	1	(R)SSRDMAGGAGPR(S)
1183.5869	1183.5629	20		606	616	0	(R) (R)
1185.5303	1185.6380	-91		102	110	0	(R) (E)
1202.5377	1202.6381	-84		443	453	0	(K) (S)
1360.5994	1360.6596	-44		123	134	0	(R) (Q)
1377.5734	1377.6439	-51		340	349	1	(K) (A)
1443.7085	1443.7120	-2.4		379	390	1	(K) (E)
1588.7634	1588.8434	-50		9	22	1	(R) (A)
1678.8185	1678.7502	41		426	439	1	(R) (A)
1716.7863	1716.8040	-10		589	605	0	(K) (T)
2705.0528	2705.2266	-64	1Met-ox	400	422	1	(K)EFGSVNGDNKPIWMHAEEREESK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (180/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 17/104 matches (16%).
Acc. #: Species: HUMAN Name: LIM and SH3 domain protein 1 (LASP-1) (MLN 50)
Index: MW: 29717 Da pI: 6.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
972.4104 972.4678 -59 86 92 1 (R) (N)
981.3547 981.4318 -79 122 128 0 (K) (S)
1185.5303 1185.5864 -47 pyroGlu 76 85 0 (K)QQSELQSQVR(Y)
1202.5377 1202.6129 -63 76 85 0 (K) (Y)
1235.5216 1235.5447 -19 1 10 1 (-) (I)
1291.5601 1291.6258 -51 50 59 0 (K) (Q)
1360.5994 1360.6836 -62 18 27 1 (K) (A)
1418.6528 1418.7280 -53 97 109 0 (K) (I)
1443.7085 1443.7920 -58 74 85 1 (R) (Y)
1493.6377 1493.7712 -89 76 87 1 (K) (E)
1505.6114 1505.6477 -24 1Met-ox 131 144 1 (R)MGPSGGEGMEPERR(D)
1521.5696 1521.6426 -48 2Met-ox 131 144 1 (R)MGPSGGEGMEPERR(D)
1576.6419 1576.6848 -27 129 143 1 (K) (R)
1603.7728 1603.8444 -45 95 109 1 (K) (I)
1607.6739 1607.7375 -40 pyroGlu 1Met-ox 60 73 0 (K)QSFTMVADTPENLR(L)
1624.6849 1624.7641 -49 1Met-ox 60 73 0 (K)QSFTMVADTPENLR(L)
1678.8185 1678.8877 -41 188 205 1 (K) (R)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
972.4104	972.4678	-59		86	92	1	(R) (N)
981.3547	981.4318	-79		122	128	0	(K) (S)
1185.5303	1185.5864	-47	pyroGlu	76	85	0	(K)QQSELQSQVR(Y)
1202.5377	1202.6129	-63		76	85	0	(K) (Y)
1235.5216	1235.5447	-19		1	10	1	(-) (I)
1291.5601	1291.6258	-51		50	59	0	(K) (Q)
1360.5994	1360.6836	-62		18	27	1	(K) (A)
1418.6528	1418.7280	-53		97	109	0	(K) (I)
1443.7085	1443.7920	-58		74	85	1	(R) (Y)
1493.6377	1493.7712	-89		76	87	1	(K) (E)
1505.6114	1505.6477	-24	1Met-ox	131	144	1	(R)MGPSGGEGMEPERR(D)
1521.5696	1521.6426	-48	2Met-ox	131	144	1	(R)MGPSGGEGMEPERR(D)
1576.6419	1576.6848	-27		129	143	1	(K) (R)
1603.7728	1603.8444	-45		95	109	1	(K) (I)
1607.6739	1607.7375	-40	pyroGlu 1Met-ox	60	73	0	(K)QSFTMVADTPENLR(L)
1624.6849	1624.7641	-49	1Met-ox	60	73	0	(K)QSFTMVADTPENLR(L)
1678.8185	1678.8877	-41		188	205	1	(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 45% (119/261AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/104 matches (8%).
Acc. #: Species: HUMAN Name: HYPOTHETICAL PROTEIN DJ1198H6.1
Index: MW: 53734 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1151.6049 1151.5122 81 107 115 0 (R) (W)
1302.5846 1302.6635 -61 166 174 0 (R) (R)
1391.6287 1391.7139 -61 435 445 1 (R) (Q)
1393.5996 1393.6574 -42 376 386 0 (R) (N)
1407.6296 1407.7088 -56 1Met-ox 435 445 1 (R)AELMCTLREVR(Q)
1603.7728 1603.9059 -83 201 213 0 (K) (N)
1851.8328 1851.8740 -22 116 131 0 (R) (R)
2017.8815 2018.0446 -81 407 424 0 (K) (V)
2232.9666 2233.0573 -41 237 254 1 (K) (L)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1151.6049	1151.5122	81		107	115	0	(R) (W)
1302.5846	1302.6635	-61		166	174	0	(R) (R)
1391.6287	1391.7139	-61		435	445	1	(R) (Q)
1393.5996	1393.6574	-42		376	386	0	(R) (N)
1407.6296	1407.7088	-56	1Met-ox	435	445	1	(R)AELMCTLREVR(Q)
1603.7728	1603.9059	-83		201	213	0	(K) (N)
1851.8328	1851.8740	-22		116	131	0	(R) (R)
2017.8815	2018.0446	-81		407	424	0	(K) (V)
2232.9666	2233.0573	-41		237	254	1	(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (105/461AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/104 matches (13%).
Acc. #: Species: MOUSE Name: LIM AND SH3 DOMAIN PROTEIN 1 (LASP-1) (MLN 50)
Index: MW: 29995 Da pI: 6.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
972.4104 972.4678 -59 86 92 1 (R) (N)
981.3547 981.4318 -79 122 128 0 (K) (S)
1185.5303 1185.5864 -47 pyroGlu 76 85 0 (K)QQSELQSQVR(Y)
1202.5377 1202.6129 -63 76 85 0 (K) (Y)
1235.5216 1235.5447 -19 1 10 1 (-) (I)
1291.5601 1291.6258 -51 50 59 0 (K) (Q)
1418.6528 1418.7280 -53 97 109 0 (K) (I)
1443.7085 1443.7920 -58 74 85 1 (R) (Y)
1493.6377 1493.7712 -89 76 87 1 (K) (E)
1544.7403 1544.7127 18 129 143 1 (K) (R)
1603.7728 1603.8444 -45 95 109 1 (K) (I)
1607.6739 1607.7375 -40 pyroGlu 1Met-ox 60 73 0 (K)QSFTMVADTPENLR(L)
1624.6849 1624.7641 -49 1Met-ox 60 73 0 (K)QSFTMVADTPENLR(L)
1678.8185 1678.8877 -41 190 207 1 (K) (R)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
972.4104	972.4678	-59		86	92	1	(R) (N)
981.3547	981.4318	-79		122	128	0	(K) (S)
1185.5303	1185.5864	-47	pyroGlu	76	85	0	(K)QQSELQSQVR(Y)
1202.5377	1202.6129	-63		76	85	0	(K) (Y)
1235.5216	1235.5447	-19		1	10	1	(-) (I)
1291.5601	1291.6258	-51		50	59	0	(K) (Q)
1418.6528	1418.7280	-53		97	109	0	(K) (I)
1443.7085	1443.7920	-58		74	85	1	(R) (Y)
1493.6377	1493.7712	-89		76	87	1	(K) (E)
1544.7403	1544.7127	18		129	143	1	(K) (R)
1603.7728	1603.8444	-45		95	109	1	(K) (I)
1607.6739	1607.7375	-40	pyroGlu 1Met-ox	60	73	0	(K)QSFTMVADTPENLR(L)
1624.6849	1624.7641	-49	1Met-ox	60	73	0	(K)QSFTMVADTPENLR(L)
1678.8185	1678.8877	-41		190	207	1	(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (108/263AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):