MS-Fit Search Results



.

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B136
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 3827 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/105(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.002e+01125 (23)54.023.820.946.9 53166/5.8HUMANTryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TRPRS) (IFP53) (hWRS)
27.634e+00612 (11)26.011.442.541.5 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
37.444e+00514 (13)26.013.315.056.9 54283/6.3MOUSETryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TrpRS)
43.642e+00510 (9)17.09.541.488.4 74650/5.2MOUSEMethylenetetrahydrofolate reductase
52.901e+00514 (13)21.013.327.891.5 82655/6.4HUMANVesicular-fusion protein NSF (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein)
62.711e+00518 (17)22.017.127.784.9 82566/6.5MOUSEVESICULAR-FUSION PROTEIN NSF (N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN) (NEM-SENSITIVE FUSION PROTEIN) (SKD2 PROTEIN)
72.093e+00516 (15)33.015.223.863.7 53675/5.5HUMANKeratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
81.593e+00512 (11)19.011.428.681.8 98621/7.8HUMANMetabotropic glutamate receptor 3 precursor (mGluR3)
91.334e+00513 (12)14.012.412.781.3 98146/7.0MOUSEDynamin 2 (Dynamin UDNM)
101.306e+00516 (15)33.015.232.936.4 51418/5.5HUMANKeratin, type II cytoskeletal 7 (Cytokeratin 7) (K7) (CK 7) (Sarcolectin)
17234.x...x..x....x..............x......x...........x.....x....x......x....x..xx.....x.x.x.x.....x....x.xxxxxx
45313.......x..x..........x....x.......x.........x.x..x.............x..........x....................x......x..
7988.x...x..x...................x.........x....x...x..........x...........x.............x............x..x.x.x
61898........x...........x.x.x.......x......x..x............x.............................................x.x.
16203..x......................x.x.........x.....xx...x..........x...x.............x.......x.......x.x....x....
38994..x...x.x..........xx....x.x.........x.....xx...x..x.......x.................x.......x....x..x......x....
27475x....xxx..x..x.........x.x...........x........x.......x...xx.........x....x.x............................
66248........x.x.x...................x...x..x......x.........................................x...x....xx.x....
26696x.........x...x..........x.....x.........xxxx.........x.....x.x.........................................x
28772xx.....x..x..x....x..x.....x.........x....xx..x.............x.....x.x..x.................................



Detailed Results

1. 25/105 matches (23%).
Acc. #: Species: HUMAN Name: Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TRPRS) (IFP53) (hWRS)
Index: MW: 53166 Da pI: 5.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
972.5598972.5267342572640(K) (G)
1000.60791000.5791291121191(K) (I)
1019.58951019.5539351341411(R) (D)
1060.59911060.58909.54504581(R) (E)
1144.65591144.6155351541620(K) (G)
1191.71511191.673735971061(K) (F)
1279.69741279.6356482212310(K) (T)
1319.75241319.7085331231330(R) (R)
1341.73741341.7643-202542641(K) (G)
1404.76361404.69714728411(K) (M)
1430.70771430.6626322652770(K) (I)
1455.70521455.6426431Met-ox 1421530(R)DMNQVLDAYENK(K)
1475.80171475.8096-5.41231341(R) (G)
1693.86071693.84718.04514641(K) (K)
1709.86631709.8420141Met-ox 4514641(K)EVTDEIVKEFMTPR(K)
1784.88731784.8377283503660(K) (Q)
1801.07131801.0336214334480(K) (R)
1929.13961929.12865.74324481(K) (R)
2084.11722084.0639261Met-ox 1631810(R)GPSSEAMHVGHLIPFIFTK(W)
2141.02552140.9248472Met-ox 2322490(K)TFIFSDLDYMGMSSGFYK(N)
2253.11662253.0684213013180(R) (M)
2325.26832325.2032282782980(K) (D)
2384.03142384.1709-592572771(K) (I)
2485.40202485.3607173273490(R) (M)
2524.22172524.1965102993181(R) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 54% (257/471AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/105 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1016.54401016.5013423563640(K) (S)
1033.55901033.5166414844920(R) (M)
1127.57721127.5407322892981(K) (V)
1141.58971141.5199614644720(R) (L)
1179.64741179.6010393773860(K) (H)
1265.67671265.6378312782880(R) (K)
1277.75981277.6337992472571(K) (N)
1277.75981277.7105394734830(K) (T)
1300.61371300.5302642582670(K) (N)
1383.72961383.6909281861970(K) (V)
1475.80171475.7494352122230(K) (T)
1475.80171475.7858112002110(R) (W)
1994.05551993.9772392242390(R) (R)
2384.03142383.9524335195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (173/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 14/105 matches (13%).
Acc. #: Species: MOUSE Name: Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TrpRS)
Index: MW: 54283 Da pI: 6.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
972.5598972.5267342612680(K) (G)
1000.60791000.5791291161231(K) (I)
1019.58951019.5539351381451(R) (D)
1144.65591144.6155351581660(K) (G)
1229.65851229.6638-4.31Met-ox 46551(K)MLLSLKMSYK(A)
1245.65191245.6587-5.52Met-ox 46551(K)MLLSLKMSYK(A)
1279.69741279.6356482252350(K) (T)
1341.73741341.7643-202582681(K) (G)
1430.70771430.6626322692810(K) (S)
1784.88731784.8377283543700(K) (Q)
2084.11722084.0639261Met-ox 1671850(R)GPSSEAMHLGHLVPFIFTK(W)
2253.11662253.0684213053220(R) (M)
2384.03142384.1709-592612811(K) (S)
2524.22172524.1965103033221(R) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (126/481AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/105 matches (9%).
Acc. #: Species: MOUSE Name: Methylenetetrahydrofolate reductase
Index: MW: 74650 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1019.58951019.5447443253330(R) (Q)
1124.58331124.5668154014080(K) (S)
1128.54261128.46576815260(R) (D)
1130.62361130.61705.868780(R) (F)
1165.63951165.5602686456541(R) (-)
1232.69021232.5734953343430(K) (R)
1242.66901242.7309-505195290(R) (T)
1325.69001325.66831658670(K) (T)
2325.26832325.097474821040(R) (E)
2485.40202485.2080783884081(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (117/654AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/105 matches (13%).
Acc. #: Species: HUMAN Name: Vesicular-fusion protein NSF (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein)
Index: MW: 82655 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
974.5653974.5093571Met-ox 2762831(K)MLNAREPK(V)
1137.57631137.5540202943030(K) (K)
1142.62641142.6574-276086170(R) (F)
1201.68101201.7170-304044130(R) (M)
1245.65191245.6666-122552660(K) (T)
1265.67671265.6490222943041(K) (L)
1291.76441291.6646774354460(K) (A)
1344.72231344.7487-201882001(K) (T)
1383.72961383.6028921051161(K) (M)
1582.96121582.8375784144271(R) (E)
1800.01391800.0206-3.72552711(K) (Q)
1930.14481929.9882815175331(R) (T)
1994.05551993.92116711270(R) (D)
2253.11662253.1445-121Met-ox 7107271(K)LLMLIEMSLQMDPEYRVR(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (159/744AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 18/105 matches (17%).
Acc. #: Species: MOUSE Name: VESICULAR-FUSION PROTEIN NSF (N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN) (NEM-SENSITIVE FUSION PROTEIN) (SKD2 PROTEIN)
Index: MW: 82566 Da pI: 6.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
974.5653974.5093571Met-ox 2762831(K)MLNAREPK(V)
1003.50971003.5107-0.99AcetN 1101(-)AGRTMQAAR(C)
1019.58951019.505682AcetN 1Met-ox 1101(-)AGRTMQAAR(C)
1108.60121108.5356591Met-ox 1101(-)MAGRTMQAAR(C)
1124.58331124.5305472Met-ox 1101(-)MAGRTMQAAR(C)
1137.57631137.5540202943030(K) (K)
1142.62641142.6574-276086170(R) (F)
1201.68101201.7170-304044130(R) (M)
1245.65191245.6666-122552660(K) (T)
1265.67671265.6490222943041(K) (L)
1291.76441291.6646774354460(K) (A)
1303.61801303.6217-2.828380(K) (T)
1344.72231344.7487-201882001(K) (T)
1582.96121582.8375784144271(R) (E)
1800.01391800.0206-3.72552711(K) (Q)
1858.08021857.9268831511681(K) (R)
1930.14481929.9882815175331(R) (T)
2253.11662253.1445-121Met-ox 7107271(K)LLMLIEMSLQMDPEYRVR(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (169/744AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 16/105 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
Index: MW: 53675 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
906.4865906.4685201111170(R) (M)
1000.60791000.5679403173250(R) (G)
1003.50971003.5424-3389961(R) (T)
1016.54401016.494748pyroGlu 1Met-ox 3653721(K)QDMARQLR(E)
1033.55901033.5213371Met-ox 3653721(K)QDMARQLR(E)
1033.55901033.6006-404554641(R) (K)
1060.59911060.5639333934011(R) (L)
1129.63191129.62179.03423521(R) (L)
1129.63191129.62179.03533620(K) (A)
1137.57631137.5792-2.52862950(K) (H)
1201.68101201.6541221491581(R) (L)
1277.75981277.7105393823920(K) (K)
1322.75401322.6453822652751(K) (A)
1341.73741341.7490-8.73173281(R) (A)
1344.72231344.6759343293410(R) (G)
1419.82151419.7484522142250(R) (Q)
1475.80171475.6987704024140(R) (T)
1566.82291566.7950181Met-ox 3703811(R)QLREYQELMNVK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (164/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 12/105 matches (11%).
Acc. #: Species: HUMAN Name: Metabotropic glutamate receptor 3 precursor (mGluR3)
Index: MW: 98621 Da pI: 7.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1019.58951019.54134780880(K) (L)
1033.55901033.5352232362440(R) (V)
1051.61071051.5159908688770(R) (-)
1165.63951165.5893431881970(R) (A)
1194.68571194.6054673573651(K) (N)
1232.69021232.7618-585150(R) (G)
1277.75981277.6598784164261(K) (K)
1815.06591814.9726516446600(R) (T)
1929.13961929.1108151Met-ox 2602751(R)ELLQKPNARVVVLFMR(S)
1929.13961929.1187118218361(K) (L)
2084.11722084.1643-2330481(R) (G)
2097.13842097.12327.21611791(R) (S)
2253.11662253.0742193343501(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (173/877AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 13/105 matches (12%).
Acc. #: Species: MOUSE Name: Dynamin 2 (Dynamin UDNM)
Index: MW: 98146 Da pI: 7.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
906.4865906.4056895555611(K) (K)
1033.55901033.5907-312292371(R) (S)
1084.66631084.6016603623691(R) (F)
1137.57631137.5845-7.32582660(K) (H)
1158.61231158.6734-533003090(K) (E)
1234.68511234.6870-1.55625711(K) (I)
1242.66901242.6768-6.35150(R) (L)
1245.65191245.6340143163251(K) (T)
1265.67671265.6795-2.22572661(R) (H)
1322.75401322.7796-195115220(R) (R)
1348.78171348.7476256586690(R) (S)
1375.81891375.7772305635731(K) (D)
2524.22172524.0534676306510(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (123/870AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 16/105 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 7 (Cytokeratin 7) (K7) (CK 7) (Sarcolectin)
Index: MW: 51418 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
906.4865906.4685201111170(R) (L)
972.5598972.5478123433511(R) (A)
1016.54401016.494748pyroGlu 1Met-ox 3663731(K)QDMARQLR(E)
1033.55901033.5213371Met-ox 3663731(K)QDMARQLR(E)
1060.59911060.5639333944021(R) (L)
1104.61711104.58023353640(R) (E)
1127.57721127.5407321992081(K) (V)
1142.62641142.5404751Met-ox 3743820(R)EYQELMSVK(L)
1201.68101201.6065623323420(K) (G)
1242.66901242.6442201501610(R) (L)
1245.65191245.64396.487961(R) (T)
1277.75981277.7105393833930(K) (K)
1348.78171348.7476251881991(R) (D)
1406.76911406.706844AcetN 1130(-)SIHFSSPVFTSR(S)
1418.75861418.7280222152260(K) (T)
1442.82491442.8007171371490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (157/469AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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