MS-Fit Search Results



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Parameters

Database searched: NCBInr.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B147
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 1030915
Molecular weight search (10000 - 40000 Da) selects 583010 entries.
Full pI range: 1030915 entries.
Species search ( HUMAN MOUSE ) selects 175330 entries.
Combined molecular weight, pI and species searches select 62216 entries.
Pre searches select 62216 entries.

Data Set 1 Results

MS-Fit search selects 2367 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/111(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.036e+0048 (7)48.07.2-0.95951.0 26306/6.0HOMO SAPIENS(BC008192) hypothetical protein FLJ20397
22.034e+0048 (7)48.07.2-0.95951.0 26334/6.0MUNREADABLEgi|8923372|ref|NP_060272.1| (NM_017802) hypothetical protein FLJ20397 [Homo sapiens]
31.983e+00411 (9)31.09.921.644.9 39742/4.7MHUMAN (FRAGMENT)keratin 10, type I, cytoskeletal (clone HK51)
451079 (8)46.08.125.633.0 23239/5.2HOMO SAPIENS(D38076) Ran-BP1(Ran-binding protein 1)
550929 (8)46.08.125.633.0 23310/5.2MUNREADABLEgi|4506407|ref|NP_002873.1| (NM_002882) RAN binding protein 1 [Homo sapiens]
649936 (5)20.05.4-2.8456.2 28663/6.5MUNREADABLEgi|21614507|ref|NP_659508.1| (NM_145071) cytokine-inducible SH2-containing protein, isoform 2; cytokine-inducible inhibitor of signaling type 1B; suppressor of cytokine signaling [Homo sapiens]
740448 (7)40.07.230.615.3 23453/5.2HUMANRan-specific GTPase-activating protein
840218 (7)40.07.230.615.3 23583/5.2MMOUSERan-specific GTPase-activating protein (Ran binding protein 1) (RANBP1)
940198 (7)40.07.230.615.3 23597/5.2MUNREADABLEgi|20346197|ref|XP_110161.1| (XM_110161) RAN binding protein 1 [Mus musculus]
1038936 (5)14.05.416.134.7 37651/7.4MHUMANfactor H homologue
316635......................x.....x......x......................................xx..........x..........x.x...........
397915......................x.....x......x......................................xx..........x..........x.x...........
824561..........x.............x..x..........x.xxx....x...x.................................x........................x
1029018.x..................x..............x.......x..........x.......x.....x...............................x.......x..
824196.x..................x..............x.......x..........x.......x.....x...............................x.......x..
426411.....x.....................................x..........x.x.................xx...................................
473668.x.................................x.......x..........x.......x.....x...............................x.......x..
765131.x.................................x.......x..........x.......x.....x...............................x.......x..
433678.x.................................x.......x..........x.......x.....x...............................x.......x..
1020049.........................................x.x........x.....................xx............x......................



Detailed Results

1. 8/111 matches (7%).
Acc. #: Species: HOMO SAPIENS Name: (BC008192) hypothetical protein FLJ20397
Index: MW: 26306 Da pI: 6.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1212.70891212.7204-9.51841940(R) (E)
1302.71451302.70586.71952060(K) (E)
1335.66041335.6909-231731830(R) (A)
1851.97651851.9526131041201(R) (L)
1868.02381867.9475411Met-ox 1041201(R)IINTFLKTSGGMTDPEK(L)
2150.13742150.1102131Met-ox 1411601(R)MAAASTLVTWLQCVKGANAK(S)
2406.12962406.2339-4355760(R) (D)
2451.20792451.2203-5.02152351(K) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 48% (114/235AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/111 matches (7%).
Acc. #: Species: UNREADABLE Name: gi|8923372|ref|NP_060272.1| (NM_017802) hypothetical protein FLJ20397 [Homo sapiens]
Acc. #: Species: HOMO SAPIENS Name: (AK000404) unnamed protein product
Index: MW: 26334 Da pI: 6.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1212.70891212.7204-9.51841940(R) (E)
1302.71451302.70586.71952060(K) (E)
1335.66041335.6909-231731830(R) (A)
1851.97651851.9526131041201(R) (L)
1868.02381867.9475411Met-ox 1041201(R)IINTFLKTSGGMTDPEK(L)
2150.13742150.1102131Met-ox 1411601(R)MAAASTLVTWLQCVKGANAK(S)
2406.12962406.2339-4355760(R) (D)
2451.20792451.2203-5.02152351(K) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 48% (114/235AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/111 matches (9%).
Acc. #: Species: HUMAN (FRAGMENT) Name: keratin 10, type I, cytoskeletal (clone HK51)
Acc. #: Species: HOMO SAPIENS Name: (M77663) keratin 10
Index: MW: 39742 Da pI: 4.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1109.54171109.4904461391470(K) (S)
1234.71621234.67963040491(R) (Q)
1300.60051300.59563.890991(K) (N)
1340.72701340.692326pyroGlu 50611(R)QSVEADINGLRR(V)
1357.69521357.7188-1750611(R) (V)
1365.67481365.6399261271370(R) (K)
1373.70391373.654936pyroGlu 1912030(K)QSLEASLAETEGR(Y)
1434.83331434.7705442442541(K) (G)
1493.79271493.7348391271381(R) (D)
2097.02832096.9810232272430(R) (I)
2872.34392872.3933-171001260(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (121/384AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/111 matches (8%).
Acc. #: Species: HOMO SAPIENS Name: (D38076) Ran-BP1(Ran-binding protein 1)
Index: MW: 23239 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1034.56121034.5271331421500(R) (F)
1186.68801186.7047-141791891(K) (E)
1335.66041335.62212958680(R) (E)
1381.68961381.63753840500(K) (M)
1620.81531620.76583158701(R) (G)
1684.82371684.7740301Met-ox 40521(K)TLEEDEEELFKMR(A)
1751.91371751.87572255681(K) (E)
2464.24912464.1423432Met-ox 981180(K)ICANHYITPMMELKPNAGSDR(A)
2753.40682753.3510201191410(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 46% (93/200AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/111 matches (8%).
Acc. #: Species: UNREADABLE Name: gi|4506407|ref|NP_002873.1| (NM_002882) RAN binding protein 1 [Homo sapiens]
Acc. #: Species: HUMAN Name: Ran-specific GTPase-activating protein (Ran binding protein 1) (RanBP1)
Acc. #: Species: HUMAN Name: GTP binding protein RanBP1
Acc. #: Species: UNREADABLE Name: gi|20150666|pdb|1K5D|B Chain B, Crystal Structure Of Ran-Gppnhp-Ranbp1-Rangap Complex
Acc. #: Species: UNREADABLE Name: gi|20150669|pdb|1K5D|E Chain E, Crystal Structure Of Ran-Gppnhp-Ranbp1-Rangap Complex
Acc. #: Species: UNREADABLE Name: gi|20150672|pdb|1K5D|H Chain H, Crystal Structure Of Ran-Gppnhp-Ranbp1-Rangap Complex
Acc. #: Species: UNREADABLE Name: gi|20150675|pdb|1K5D|K Chain K, Crystal Structure Of Ran-Gppnhp-Ranbp1-Rangap Complex
Acc. #: Species: UNREADABLE Name: gi|20150678|pdb|1K5G|B Chain B, Crystal Structure Of Ran-Gdp-Alfx-Ranbp1-Rangap Complex
Acc. #: Species: UNREADABLE Name: gi|20150681|pdb|1K5G|E Chain E, Crystal Structure Of Ran-Gdp-Alfx-Ranbp1-Rangap Complex
Acc. #: Species: UNREADABLE Name: gi|20150684|pdb|1K5G|H Chain H, Crystal Structure Of Ran-Gdp-Alfx-Ranbp1-Rangap Complex
Acc. #: Species: UNREADABLE Name: gi|20150687|pdb|1K5G|K Chain K, Crystal Structure Of Ran-Gdp-Alfx-Ranbp1-Rangap Complex
Acc. #: Species: HOMO SAPIENS Name: (X83617) RanBP1
Index: MW: 23310 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1034.56121034.5271331421500(R) (F)
1186.68801186.7047-141801901(K) (E)
1335.66041335.62212958680(R) (E)
1381.68961381.63753840500(K) (M)
1620.81531620.76583158701(R) (G)
1684.82371684.7740301Met-ox 40521(K)TLEEDEEELFKMR(A)
1751.91371751.87572255681(K) (E)
2464.24912464.1423432Met-ox 981180(K)ICANHYITPMMELKPNAGSDR(A)
2753.40682753.3510201191410(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 46% (93/201AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 6/111 matches (5%).
Acc. #: Species: UNREADABLE Name: gi|21614507|ref|NP_659508.1| (NM_145071) cytokine-inducible SH2-containing protein, isoform 2; cytokine-inducible inhibitor of signaling type 1B; suppressor of cytokine signaling [Homo sapiens]
Acc. #: Species: HUMAN Name: Cytokine-inducible SH2-containing protein (CIS) (CIS-1) (Suppressor of cytokine signaling) (SOCS) (G18)
Acc. #: Species: HOMO SAPIENS Name: (D83532) cytokine-inducible SH2-containing protein
Acc. #: Species: HOMO SAPIENS Name: (BC031590) cytokine inducible SH2-containing protein
Index: MW: 28663 Da pI: 6.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1065.56651065.5403251Met-ox 991070(K)MPEGTFLVR(D)
1381.68961381.7513-452342450(R) (R)
1620.81531620.7943131731880(R) (E)
1636.82161636.7892201Met-ox 1731880(R)SDSPDPAPTPALPMPK(E)
1851.97651852.0301-291160(-) (T)
1868.02381868.0250-0.661Met-ox 1160(-)MVLCVQGPRPLLAVER(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (53/258AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 8/111 matches (7%).
Acc. #: Species: HUMAN Name: Ran-specific GTPase-activating protein
Index: MW: 23453 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1034.56121034.5271331411490(R) (F)
1335.66041335.62212957670(R) (E)
1381.68961381.63753839490(K) (M)
1620.81531620.76583157691(R) (G)
1684.82371684.7740301Met-ox 39511(K)TLEEDEEELFKMR(A)
1751.91371751.87572254671(K) (E)
2464.24912464.1423432Met-ox 971170(K)ICANHYITPMMELKPNAGSDR(A)
2753.40682753.3510201181400(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (82/202AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 8/111 matches (7%).
Acc. #: Species: MOUSE Name: Ran-specific GTPase-activating protein (Ran binding protein 1) (RANBP1)
Acc. #: Species: MUS MUSCULUS Name: (L25255) Ran/TC4 Binding Protein
Acc. #: Species: HOMO SAPIENS Name: Ran/TC4-binding protein 1
Index: MW: 23583 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1034.56121034.5271331421500(R) (F)
1335.66041335.62212958680(R) (E)
1381.68961381.63753840500(K) (M)
1620.81531620.76583158701(R) (G)
1684.82371684.7740301Met-ox 40521(K)TLEEDEEELFKMR(A)
1751.91371751.87572255681(K) (E)
2464.24912464.1423432Met-ox 981180(K)ICANHYITPMMELKPNAGSDR(A)
2753.40682753.3510201191410(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (82/203AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/111 matches (7%).
Acc. #: Species: UNREADABLE Name: gi|20346197|ref|XP_110161.1| (XM_110161) RAN binding protein 1 [Mus musculus]
Acc. #: Species: UNREADABLE Name: gi|20891965|ref|XP_147234.1| (XM_147234) RAN binding protein 1 [Mus musculus]
Acc. #: Species: MUS MUSCULUS Name: (AK002989) RAN binding protein 1~data source:MGD, source key:MGI:96269, evidence:ISS~putative
Index: MW: 23597 Da pI: 5.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1034.56121034.5271331421500(R) (F)
1335.66041335.62212958680(R) (E)
1381.68961381.63753840500(K) (M)
1620.81531620.76583158701(R) (G)
1684.82371684.7740301Met-ox 40521(K)TLEEDEEELFKMR(A)
1751.91371751.87572255681(K) (E)
2464.24912464.1423432Met-ox 981180(K)ICANHYITPMMELKPNAGSDR(A)
2753.40682753.3510201191410(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (82/203AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 6/111 matches (5%).
Acc. #: Species: HUMAN Name: factor H homologue
Acc. #: Species: HOMO SAPIENS Name: (M65293) factor H homologue
Acc. #: Species: HOMO SAPIENS Name: (BC016755) H factor (complement)-like 1
Index: MW: 37651 Da pI: 7.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1365.67481365.6837-6.52712810(R) (W)
1381.68961381.67868.01Met-ox 2712810(R)EIMENYNIALR(W)
1519.72841519.71031270820(R) (C)
1851.97651851.9427182712851(R) (Q)
1868.02381867.9376461Met-ox 2712851(R)EIMENYNIALRWTAK(Q)
2211.12472211.0828191421610(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (48/330AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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