MS-Fit Search Results



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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B150
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 2563 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/98(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.832e+00916 (16)55.016.3-20.334.2 38715/6.6HUMANAnnexin I (Lipocortin I) (Calpactin II) (Chromobindin 9) (P35) (Phospholipase A2 inhibitory protein)
23.412e+00712 (12)32.012.2-4.4575.9 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
38.366e+00510 (10)41.010.2-21.924.6 34274/5.7HUMAN60S acidic ribosomal protein P0 (L10E)
45.059e+00513 (13)21.013.313.380.5 68935/6.7HUMANKinesin light chain 2 (KLC 2)
51.259e+00513 (13)42.013.3-18.346.1 36054/8.6HUMANGlyceraldehyde 3-phosphate dehydrogenase, liver
61.213e+0059 (9)36.09.2-21.626.0 34217/5.9MOUSE60S acidic ribosomal protein P0 (L10E)
79.956e+00412 (12)18.012.23.2682.7 96306/5.6MOUSETuftelin-interacting protein 11 (Tuftelin-interacting protein 39)
88.140e+00411 (11)20.011.2-30.979.8 92273/5.5HUMANMucosa associated lymphoid tissue lymphoma translocation protein 1 (MALT-lymphoma associated translocation) (Paracaspase)
97.383e+00410 (10)16.010.2-3.1965.1 93559/7.8HUMANAxin 2 (Axis inhibition protein 2) (Conductin) (Axin-like protein) (Axil)
107.319e+00411 (11)32.011.2-20.768.8 39180/10.0HUMANMelanoma-associated antigen B3 (MAGE-B3 antigen)
52500...x...x.....x.....x.........x..........xx.x..x.......x..x.....x.............x.......x...x....x...
45313......x....x..x.......x...x..........x..............x..x.....x.......x...........x.............x..
65522..............xx....x....x..x...x....................x..................x...x.............x.......
87113.x.xx.x.x...x..x...................x....x....x...............x..x...........x.....................
108737..............x.x.............x...........x.....x.................x........x......x........xxx..xx
17489..............xx....x....x..x...x....................x..................x.................x.......
31132x.......x.........x.......xx.x.......xx.........................x.......xx..x.....................
24819..........................x...........xxx.....xx.....x.................x...............xx..x......
14223........................x...x.........xx...........x.........x....x........x........x.x...........
38578x..x.x..xx....x.x.........................................x...........x...................xx......



Detailed Results

1. 16/98 matches (16%).
Acc. #: Species: HUMAN Name: Annexin I (Lipocortin I) (Calpactin II) (Chromobindin 9) (P35) (Phospholipase A2 inhibitory protein)
Index: MW: 38715 Da pI: 6.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
982.4900982.4919-2.0AcetN 1Met-ox 190(-)AMVSEFLK(Q)
1099.52961099.5972-6173811(R) (A)
1213.54721213.5337111671770(K) (N)
1262.59511262.6017-5.21141240(K) (A)
1387.74571387.7684-1659710(K) (R)
1522.71501522.7576-283333461(K) (-)
1543.82931543.8695-2659721(K) (N)
1550.78911550.8178-192152280(K) (S)
1605.89411605.9580-40991130(K) (T)
1678.89091678.9128-132142281(R) (S)
1702.86711702.8863-111291440(K) (T)
1739.72341739.7360-7.31892040(R) (A)
1904.98471905.0333-2682981(K) (A)
2067.85242067.8855-161862041(K) (A)
2140.94412141.0191-3510260(K) (S)
2356.08722356.1573-3030530(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 55% (191/346AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/98 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1092.58211092.5034726036160(R) (G)
1179.59681179.6010-3.53773860(K) (H)
1217.62751217.61669.06346440(R) (-)
1302.62521302.7018-593934031(R) (V)
1302.62521302.7157-693443550(R) (A)
1316.60061316.5251571Met-ox 2582670(K)NMQDMVEDYR(N)
1475.74931475.7494-0.0932122230(K) (T)
1475.74931475.7858-252002110(R) (W)
1657.79921657.79343.513290(R) (R)
1687.92401687.9707-2866821(R) (G)
1716.84441716.8517-4.34184320(K) (G)
1779.77911779.75091646650(R) (S)
1993.93991993.9772-192242390(R) (R)
2383.92222383.9524-135195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (208/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/98 matches (10%).
Acc. #: Species: HUMAN Name: 60S acidic ribosomal protein P0 (L10E)
Index: MW: 34274 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1217.62751217.6782-4217260(K) (C)
1221.61891221.6228-3.267770(R) (L)
1280.61741280.6309-1127380(K) (Q)
1313.69711313.7105-101351460(K) (I)
1350.65561350.7060-3751621(K) (K)
1428.80791428.8314-161501620(R) (T)
1676.81731676.8529-211Met-ox 931061(K)EDLTEIRDMLLANK(V)
1824.89301824.9496-3184991(R) (D)
1895.95461896.0006-242482640(R) (V)
2194.06762194.1178-231131340(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (132/317AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/98 matches (13%).
Acc. #: Species: HUMAN Name: Kinesin light chain 2 (KLC 2)
Index: MW: 68935 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
937.5163937.5028141Met-ox 5565631(R)SSEMLVKK(L)
982.4900982.467723180(-) (E)
1030.43961030.4524-123Met-ox 180(-)MAMMVFPR(E)
1092.58211092.57635.22142220(R) (Q)
1092.58211092.6166-323493571(R) (L)
1151.63741151.5771522983061(K) (R)
1185.57321185.6380-551021100(R) (E)
1221.61891221.5428623403480(K) (R)
1443.75611443.7120313793901(K) (E)
1522.71501522.6490434274390(R) (A)
1588.74801588.8434-609221(R) (A)
1716.84441716.8040235896050(K) (T)
1756.88071756.8401234654791(K) (K)
1895.95461895.8274674274421(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (135/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 13/98 matches (13%).
Acc. #: Species: HUMAN Name: Glyceraldehyde 3-phosphate dehydrogenase, liver
Index: MW: 36054 Da pI: 8.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1217.62751217.6022211Met-ox 3243340(R)VVDLMAHMASK(E)
1233.60191233.59723.92Met-ox 3243340(R)VVDLMAHMASK(E)
1411.75261411.7909-272012150(R) (A)
1544.80061544.8107-6.52352480(R) (L)
1613.87011613.9015-1967800(K) (D)
1763.77741763.8029-143103230(K) (V)
1861.91231861.9516-211461620(K) (V)
2041.09572041.1082-6.167841(K) (I)
2211.05392211.2171-742352541(R) (Y)
2229.07292229.1048-141Met-ox 1191390(R)VIISAPSADAPMFVMGVNHEK(Y)
2245.07392245.0997-122Met-ox 1191390(R)VIISAPSADAPMFVMGVNHEK(Y)
2401.08862401.2008-472Met-ox 1181391(K)RVIISAPSADAPMFVMGVNHEK(Y)
2611.30122611.3554-211Met-ox 1631860(K)VIHDNFGIVEGLMTTVHAITATQK(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (141/335AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 9/98 matches (9%).
Acc. #: Species: MOUSE Name: 60S acidic ribosomal protein P0 (L10E)
Index: MW: 34217 Da pI: 5.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1217.62751217.6782-4217260(K) (C)
1221.61891221.6228-3.267770(R) (L)
1280.61741280.6309-1127380(K) (Q)
1313.69711313.7105-101351460(K) (I)
1350.65561350.7060-3751621(K) (K)
1428.80791428.8314-161501620(R) (T)
1676.81731676.8529-211Met-ox 931061(K)EDLTEIRDMLLANK(V)
1824.89301824.9496-3184991(R) (D)
2194.06762194.1178-231131340(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (115/317AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 12/98 matches (12%).
Acc. #: Species: MOUSE Name: Tuftelin-interacting protein 11 (Tuftelin-interacting protein 39)
Index: MW: 96306 Da pI: 5.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
922.3828922.3947-134124170(K) (L)
1151.63741151.5809491871981(K) (T)
1259.68861259.6523292392481(K) (A)
1316.60061316.5581327437520(K) (R)
1323.64721323.58174959700(R) (R)
1387.74571387.73686.43773871(K) (M)
1475.74931475.7892-272632751(K) (E)
1493.65621493.7438-594814910(R) (N)
1756.88071756.9750-548018141(K) (G)
1824.89301824.8373313884030(R) (I)
1834.98981835.0431-298088231(R) (T)
1895.95461895.8387611321481(K) (H)
1895.95461896.0191-343603761(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (158/838AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 11/98 matches (11%).
Acc. #: Species: HUMAN Name: Mucosa associated lymphoid tissue lymphoma translocation protein 1 (MALT-lymphoma associated translocation) (Paracaspase)
Index: MW: 92273 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1316.60061316.6785-596626711(K) (L)
1316.60061316.6884-672272370(K) (L)
1493.65621493.7344-5269800(R) (V)
1519.81611519.80557.06666771(K) (L)
1522.71501522.8052-591Met-ox 3423541(K)DKVALLIGNMNYR(E)
1605.89411605.8012584684810(R) (V)
1608.77361608.7963-141501620(R) (M)
1676.81731676.8781-361Met-ox 5245381(K)KITVLLDEVAEDMGK(C)
1795.75571795.8714-646456611(K) (D)
2106.10132106.08318.75585761(K) (N)
2113.97132114.1241-7281991(K) (G)
2211.05392211.0976-204484651(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (167/824AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 10/98 matches (10%).
Acc. #: Species: HUMAN Name: Axin 2 (Axis inhibition protein 2) (Conductin) (Axin-like protein) (Axil)
Index: MW: 93559 Da pI: 7.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1307.67261307.5949597157241(R) (N)
1350.65561350.7130-425966100(R) (E)
1493.65621493.6695-8.95615740(K) (G)
1519.81611519.7505436436561(K) (A)
1636.86591636.8923-163403541(R) (T)
1716.84441716.9046-353433571(K) (L)
1763.77741763.8048-164004141(R) (E)
1861.91231861.9370-133613770(K) (L)
2055.86792055.8403135395540(R) (C)
2096.95032096.9849-1658771(R) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (139/843AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 11/98 matches (11%).
Acc. #: Species: HUMAN Name: Melanoma-associated antigen B3 (MAGE-B3 antigen)
Index: MW: 39180 Da pI: 10.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
922.3828922.3940-122152220(K) (I)
982.4900982.5070-172622700(R) (Y)
1045.54051045.6080-641351431(K) (S)
1151.63741151.62877.52862941(K) (V)
1162.63371162.62943.61471551(K) (A)
1217.62751217.5948271841940(K) (G)
1233.60191233.58989.81Met-ox 1841940(K)MDLPNNGTVTR(G)
1706.74501706.8318-513183341(R) (C)
1706.74501706.8614-682572701(K) (Y)
1791.75231791.8818-722712851(R) (M)
2194.06762194.06013.4pyroGlu 16351(R)QQTRGQTQDHQGAQITATNK(K)
2211.05392211.0866-1516351(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (112/346AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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