MS-Fit Search Results

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Sample ID (comment): C003
Database searched: SwissProt.7.25.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 471 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |

4	50.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.13e+008	20/46 (43%)	70871.6 / 5.38	MOUSE	HEAT SHOCK COGNATE 71 KDA PROTEIN
2	2.13e+008	20/46 (43%)	70898.7 / 5.38	HUMAN	HEAT SHOCK COGNATE 71 KDA PROTEIN
3	789	6/46 (13%)	27136.9 / 5.31	MOUSE	SPINDLIN (30000 MR METAPHASE COMPLEX) (SSEC P)
4	760	8/46 (17%)	69741.4 / 5.58	MOUSE	HEAT SHOCK-RELATED 70 KD PROTEIN 2 (HEAT SHOCK PROTEIN 70.2)
5	703	8/46 (17%)	70021.5 / 5.56	HUMAN	HEAT SHOCK-RELATED 70 KDA PROTEIN 2 (HEAT SHOCK 70 KDA PROTEIN 2)

Detailed Results

1. 20/46 matches (43%). 70871.6 Da, pI = 5.38. Acc. # P08109. MOUSE. HEAT SHOCK COGNATE 71 KDA PROTEIN.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1081.5226	1081.5682	-42.1825	349	357	(K)(E)
1199.6454	1199.6748	-24.4813	160	171	(K)(I)
1228.6150	1228.6286	-11.0210	26	36	(K)(T)
1235.6086	1235.6247	-13.0179	237	246	(R)(R)
1251.5886	1251.6196	-24.7406	237	246	(R)MVNHFIAEFK(R)	1Met-ox
1253.5971	1253.6166	-15.6148	302	311	(R)(G)
1319.5579	1319.5942	-27.4887	540	550	(K)NSLESYAFNMK(A)	1Met-ox
1426.6363	1426.6637	-19.1408	77	88	(R)RFDDAVVQSDMK(H)	1Met-ox
1480.7468	1480.7549	-5.4606	300	311	(R)(G)
1487.6720	1487.7018	-20.0525	37	49	(R)(L)
1653.8199	1653.8324	-7.5562	89	102	(K)(V)
1669.8098	1669.8273	-10.4719	89	102	(K)HWPFMVVNDAGRPK(V)	1Met-ox
1691.7039	1691.7261	-13.1436	221	236	(K)(M)
1745.7903	1745.8095	-10.9618	584	597	(K)(E)
1787.9441	1787.9907	-26.0650	172	188	(R)(V)
1981.9909	1981.9983	-3.7692	138	155	(K)(Q)
1981.9909	1981.9653	12.8883	518	533	(R)(D)
2260.1252	2260.1461	-9.2684	362	384	(K)(S)
2277.1460	2277.1338	5.3553	4	25	(K)(V)
2774.3456	2774.3273	6.5826	424	447	(K)(A)
2778.2951	2778.3912	-34.5929	103	126	(K)(M)

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 43% (278/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 43.5 Mean Error = -14.0097 ppm Data Tolerance = 26.9508 ppm Mean Missed Cleavages = 0.4

2. 20/46 matches (43%). 70898.7 Da, pI = 5.38. Acc. # P11142. HUMAN. HEAT SHOCK COGNATE 71 KDA PROTEIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1081.5226 1081.5682 -42.1825 349 357 (K)(E)
1199.6454 1199.6748 -24.4813 160 171 (K)(I)
1228.6150 1228.6286 -11.0210 26 36 (K)(T)
1235.6086 1235.6247 -13.0179 237 246 (R)(R)
1251.5886 1251.6196 -24.7406 237 246 (R)MVNHFIAEFK(R) 1Met-ox
1253.5971 1253.6166 -15.6148 302 311 (R)(G)
1319.5579 1319.5942 -27.4887 540 550 (K)NSLESYAFNMK(A) 1Met-ox
1426.6363 1426.6637 -19.1408 77 88 (R)RFDDAVVQSDMK(H) 1Met-ox
1480.7468 1480.7549 -5.4606 300 311 (R)(G)
1487.6720 1487.7018 -20.0525 37 49 (R)(L)
1653.8199 1653.8324 -7.5562 89 102 (K)(V)
1669.8098 1669.8273 -10.4719 89 102 (K)HWPFMVVNDAGRPK(V) 1Met-ox
1691.7039 1691.7261 -13.1436 221 236 (K)(M)
1745.7903 1745.8095 -10.9618 584 597 (K)(E)
1787.9441 1787.9907 -26.0650 172 188 (R)(V)
1787.9441 1787.9366 4.2172 570 583 (K)(N)
1981.9909 1981.9983 -3.7692 138 155 (K)(Q)
1981.9909 1981.9653 12.8883 518 533 (R)(D)
2260.1252 2260.1461 -9.2684 362 384 (K)(S)
2277.1460 2277.1338 5.3553 4 25 (K)(V)
2774.3456 2774.3273 6.5826 424 447 (K)(A)
2778.2951 2778.3912 -34.5929 103 126 (K)(M)

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 45% (292/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 43.5 Mean Error = -13.1812 ppm Data Tolerance = 27.4255 ppm Mean Missed Cleavages = 0.5

3. 6/46 matches (13%). 27136.9 Da, pI = 5.31. Acc. # Q61142. MOUSE. SPINDLIN (30000 MR METAPHASE COMPLEX) (SSEC P).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1228.6150 1228.6425 -22.3494 183 194 (R)(Q)
1235.6086 1235.5513 46.4066 1 12 (-)(K)
1251.5886 1251.5462 33.9237 1 12 (-)MASASSPASCPR(K) 1Met-ox
1253.5971 1253.6014 -3.4452 195 205 (K)(R)
1428.6353 1428.6429 -5.3240 170 182 (R)(E)
1444.6732 1444.6378 24.5115 170 182 (R)IMPDSNDSPPAER(E) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1228.6150	1228.6425	-22.3494	183	194	(R)(Q)
1235.6086	1235.5513	46.4066	1	12	(-)(K)
1251.5886	1251.5462	33.9237	1	12	(-)MASASSPASCPR(K)	1Met-ox
1253.5971	1253.6014	-3.4452	195	205	(K)(R)
1428.6353	1428.6429	-5.3240	170	182	(R)(E)
1444.6732	1444.6378	24.5115	170	182	(R)IMPDSNDSPPAER(E)	1Met-ox

The matched peptides cover 20% (48/240AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.0 Mean Error = 12.2872 ppm Data Tolerance = 53.2094 ppm Mean Missed Cleavages = 0.2

4. 8/46 matches (17%). 69741.4 Da, pI = 5.58. Acc. # P17156. MOUSE. HEAT SHOCK-RELATED 70 KD PROTEIN 2 (HEAT SHOCK PROTEIN 70.2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1081.5226 1081.5682 -42.1825 352 360 (K)(E)
1228.6150 1228.6286 -11.0210 27 37 (K)(T)
1253.5971 1253.6166 -15.6148 305 314 (R)(G)
1480.7468 1480.7549 -5.4606 303 314 (R)(G)
1487.6720 1487.7018 -20.0525 38 50 (R)(L)
1691.7039 1691.7261 -13.1436 224 239 (K)(M)
1787.9441 1787.9907 -26.0650 173 189 (R)(G)
1993.9637 1994.0493 -42.9168 73 89 (K)(H)
1993.9637 1993.9231 20.4044 79 94 (K)(V)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1081.5226	1081.5682	-42.1825	352	360	(K)(E)
1228.6150	1228.6286	-11.0210	27	37	(K)(T)
1253.5971	1253.6166	-15.6148	305	314	(R)(G)
1480.7468	1480.7549	-5.4606	303	314	(R)(G)
1487.6720	1487.7018	-20.0525	38	50	(R)(L)
1691.7039	1691.7261	-13.1436	224	239	(K)(M)
1787.9441	1787.9907	-26.0650	173	189	(R)(G)
1993.9637	1994.0493	-42.9168	73	89	(K)(H)
1993.9637	1993.9231	20.4044	79	94	(K)(V)

The matched peptides cover 15% (100/633AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.4 Mean Error = -17.3392 ppm Data Tolerance = 38.6075 ppm Mean Missed Cleavages = 0.7

5. 8/46 matches (17%). 70021.5 Da, pI = 5.56. Acc. # P54652. HUMAN. HEAT SHOCK-RELATED 70 KDA PROTEIN 2 (HEAT SHOCK 70 KDA PROTEIN 2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1081.5226 1081.5682 -42.1825 352 360 (K)(E)
1228.6150 1228.6286 -11.0210 27 37 (K)(T)
1253.5971 1253.6166 -15.6148 305 314 (R)(G)
1480.7468 1480.7549 -5.4606 303 314 (R)(G)
1487.6720 1487.7018 -20.0525 38 50 (R)(L)
1691.7039 1691.7261 -13.1436 224 239 (K)(M)
1787.9441 1787.9907 -26.0650 173 189 (R)(G)
1993.9637 1994.0493 -42.9168 73 89 (K)(H)
1993.9637 1993.9231 20.4044 79 94 (K)(V)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1081.5226	1081.5682	-42.1825	352	360	(K)(E)
1228.6150	1228.6286	-11.0210	27	37	(K)(T)
1253.5971	1253.6166	-15.6148	305	314	(R)(G)
1480.7468	1480.7549	-5.4606	303	314	(R)(G)
1487.6720	1487.7018	-20.0525	38	50	(R)(L)
1691.7039	1691.7261	-13.1436	224	239	(K)(M)
1787.9441	1787.9907	-26.0650	173	189	(R)(G)
1993.9637	1994.0493	-42.9168	73	89	(K)(H)
1993.9637	1993.9231	20.4044	79	94	(K)(V)

The matched peptides cover 15% (100/639AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.4 Mean Error = -17.3392 ppm Data Tolerance = 38.6075 ppm Mean Missed Cleavages = 0.7