MS-Fit Search Results

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Sample ID (comment): C005
Database searched: SwissProt.7.25.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 1769 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |

4	75.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.7e+008	26/82 (31%)	70052.8 / 5.48	HUMAN	HEAT SHOCK 70 KDA PROTEIN 1 (HSP70.1) (HSP70-1/HSP70-2)
2	1.67e+005	17/82 (20%)	69994.6 / 5.46	MOUSE	HEAT SHOCK 70 KDA PROTEIN 1 (HSP70.1) (HSP70-1/HSP70-2)
3	1.68e+004	11/82 (13%)	70695.9 / 5.82	MOUSE	HEAT SHOCK-RELATED 70 KD PROTEIN
4	8.68e+003	9/82 (10%)	52164.7 / 5.37	HUMAN	RAS-GTPASE-ACTIVATING PROTEIN BINDING PROTEIN 1 (GAP SH3-DOMAIN BINDING PROTEIN 1) (G3BP-1)
5	8.08e+003	14/82 (17%)	70399.7 / 5.76	HUMAN	HEAT SHOCK 70 KDA PROTEIN 1-HOM (HSP70-HOM)

Detailed Results

1. 26/82 matches (31%). 70052.8 Da, pI = 5.48. Acc. # P08107. HUMAN. HEAT SHOCK 70 KDA PROTEIN 1 (HSP70.1) (HSP70-1/HSP70-2).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1109.4999	1109.5744	-67.0701	349	357	(K)(D)
1131.5004	1131.5394	-34.5072	527	535	(K)(V)
1137.4957	1137.5540	-51.2554	525	533	(K)(E)
1197.6094	1197.6955	-71.9640	160	171	(K)(I)
1228.5511	1228.6286	-63.0424	26	36	(K)(T)
1232.5501	1232.6421	-74.6374	448	458	(R)(F)
1248.6170	1248.6370	-16.0878	448	458	(R)AMTKDNNLLGR(F)	1Met-ox
1261.5897	1261.6581	-54.2000	237	246	(R)(R)
1287.5086	1287.6043	-74.3759	540	550	(K)(S)
1303.5173	1303.5993	-62.8862	540	550	(K)NALESYAFNMK(S)	1Met-ox
1329.5517	1329.6149	-47.5592	302	311	(R)(S)
1417.6925	1417.7592	-47.0566	237	247	(R)(K)
1465.7451	1465.8127	-46.1226	329	342	(K)(I)
1487.6335	1487.7018	-45.8937	37	49	(R)(L)
1506.6404	1506.7110	-46.8687	513	524	(K)EEIERMVQEAEK(Y)	1Met-ox
1556.6863	1556.7531	-42.9280	300	311	(R)(S)
1658.7761	1658.8502	-44.6718	57	71	(K)(R)
1675.6591	1675.7312	-43.0617	221	236	(K)(L)
1680.7748	1680.8498	-44.6350	89	102	(K)(V)
1687.8359	1687.9019	-39.0787	172	187	(R)(T)
2279.0954	2279.1494	-23.6997	4	25	(K)(V)
2320.1365	2320.1495	-5.5922	362	384	(K)SINPDEAVAYGAAVQAAILMGDK(S)	1Met-ox
2609.3126	2609.3220	-3.6142	1	25	(-)(V)
2609.3126	2609.2517	23.3162	513	533	(K)(E)
2786.3449	2786.3637	-6.7433	424	447	(K)(A)
2786.3449	2786.4035	-21.0112	536	561	(R)(I)
3001.4898	3001.4947	-1.6367	129	155	(K)(Q)
3277.6596	3277.7908	-40.0468	156	187	(R)QATKDAGVIAGLNVLRIINEPTAAAIAYGLDR(T)	pyroGlu

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 47% (306/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 31.7 Mean Error = -39.1761 ppm Data Tolerance = 48.8553 ppm Mean Missed Cleavages = 0.6

2. 17/82 matches (20%). 69994.6 Da, pI = 5.46. Acc. # P17879. MOUSE. HEAT SHOCK 70 KDA PROTEIN 1 (HSP70.1) (HSP70-1/HSP70-2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1109.4999 1109.5744 -67.0701 349 357 (K)(D)
1137.4957 1137.5540 -51.2554 525 533 (R)(D)
1179.5321 1179.6122 -67.9234 103 112 (K)(S)
1197.6094 1197.6955 -71.9640 160 171 (K)(I)
1228.5511 1228.6286 -63.0424 26 36 (K)(T)
1287.5086 1287.6043 -74.3759 540 550 (K)(S)
1303.5173 1303.5993 -62.8862 540 550 (K)NALESYAFNMK(S) 1Met-ox
1329.5517 1329.6149 -47.5592 302 311 (R)(G)
1487.6335 1487.7018 -45.8937 37 49 (R)(L)
1556.6863 1556.7531 -42.9280 300 311 (R)(G)
1658.7761 1658.8502 -44.6718 57 71 (K)(R)
1675.6591 1675.7312 -43.0617 221 236 (K)(L)
1687.8359 1687.9019 -39.0787 172 187 (R)(T)
1862.8614 1862.9282 -35.8812 510 524 (R)LSKEEIERMVQEAER(Y) 1Met-ox
2320.1365 2320.1495 -5.5922 362 384 (K)SINPDEAVAYGAAVQAAILMGDK(S) 1Met-ox
2786.3449 2786.4035 -21.0112 536 561 (R)VAAKNALESYAFNMKSAVEDEGLKGK(L) 1Met-ox
3277.6596 3277.7908 -40.0468 156 187 (R)QATKDAGVIAGLNVLRIINEPTAAAIAYGLDR(T) pyroGlu

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 29% (191/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.7 Mean Error = -48.4848 ppm Data Tolerance = 36.6312 ppm Mean Missed Cleavages = 0.6

3. 11/82 matches (13%). 70695.9 Da, pI = 5.82. Acc. # P16627. MOUSE. HEAT SHOCK-RELATED 70 KD PROTEIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1197.6094 1197.6955 -71.9640 162 173 (K)(I)
1228.5511 1228.6286 -63.0424 28 38 (K)(T)
1287.5086 1287.6043 -74.3759 542 552 (K)(S)
1303.5173 1303.5993 -62.8862 542 552 (K)NALESYAFNMK(S) 1Met-ox
1465.7451 1465.8491 -70.9435 331 344 (K)(I)
1475.6835 1475.7238 -27.2856 600 611 (K)(L)
1487.6335 1487.7018 -45.8937 39 51 (R)(L)
1675.6591 1675.7312 -43.0617 223 238 (K)(L)
1862.8614 1862.9798 -63.6001 75 90 (R)LIGRKFNDPVVQSDMK(L) 1Met-ox
1862.8614 1862.9282 -35.8812 512 526 (R)LSKEEIERMVQEAER(Y) 1Met-ox
2320.1365 2320.1495 -5.5922 364 386 (K)SINPDEAVAYGAAVQAAILMGDK(S) 1Met-ox
2786.3449 2786.3637 -6.7433 426 449 (K)(A)
2786.3449 2786.4035 -21.0112 538 563 (K)IAAKNALESYAFNMKSAVGDEGLKDK(I) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1197.6094	1197.6955	-71.9640	162	173	(K)(I)
1228.5511	1228.6286	-63.0424	28	38	(K)(T)
1287.5086	1287.6043	-74.3759	542	552	(K)(S)
1303.5173	1303.5993	-62.8862	542	552	(K)NALESYAFNMK(S)	1Met-ox
1465.7451	1465.8491	-70.9435	331	344	(K)(I)
1475.6835	1475.7238	-27.2856	600	611	(K)(L)
1487.6335	1487.7018	-45.8937	39	51	(R)(L)
1675.6591	1675.7312	-43.0617	223	238	(K)(L)
1862.8614	1862.9798	-63.6001	75	90	(R)LIGRKFNDPVVQSDMK(L)	1Met-ox
1862.8614	1862.9282	-35.8812	512	526	(R)LSKEEIERMVQEAER(Y)	1Met-ox
2320.1365	2320.1495	-5.5922	364	386	(K)SINPDEAVAYGAAVQAAILMGDK(S)	1Met-ox
2786.3449	2786.3637	-6.7433	426	449	(K)(A)
2786.3449	2786.4035	-21.0112	538	563	(K)IAAKNALESYAFNMKSAVGDEGLKDK(I)	1Met-ox

The matched peptides cover 28% (182/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.4 Mean Error = -45.5601 ppm Data Tolerance = 49.1390 ppm Mean Missed Cleavages = 0.6

4. 9/82 matches (10%). 52164.7 Da, pI = 5.37. Acc. # Q13283. HUMAN. RAS-GTPASE-ACTIVATING PROTEIN BINDING PROTEIN 1 (GAP SH3-DOMAIN BINDING PROTEIN 1) (G3BP-1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1210.5348 1210.6009 -54.5781 124 132 (K)(Y)
1248.6170 1248.6887 -57.4439 394 403 (K)VLSNRPIMFR(G) 1Met-ox
1573.7036 1573.7651 -39.0383 358 370 (K)(I)
1862.8614 1862.9223 -32.7286 18 32 (R)(F)
1878.8870 1878.9172 -16.0744 18 32 (R)QYYTLLNQAPDMLHR(F) 1Met-ox
1937.8721 1937.9649 -47.9117 377 393 (K)(V)
2540.2971 2540.2898 2.8754 336 357 (R)(D)
2609.3126 2609.3912 -30.1403 277 299 (K)(D)
3001.4898 3001.5278 -12.6471 79 106 (R)HVDAHATLNDGVVVQVMGLLSNNNQALR(R) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1210.5348	1210.6009	-54.5781	124	132	(K)(Y)
1248.6170	1248.6887	-57.4439	394	403	(K)VLSNRPIMFR(G)	1Met-ox
1573.7036	1573.7651	-39.0383	358	370	(K)(I)
1862.8614	1862.9223	-32.7286	18	32	(R)(F)
1878.8870	1878.9172	-16.0744	18	32	(R)QYYTLLNQAPDMLHR(F)	1Met-ox
1937.8721	1937.9649	-47.9117	377	393	(K)(V)
2540.2971	2540.2898	2.8754	336	357	(R)(D)
2609.3126	2609.3912	-30.1403	277	299	(K)(D)
3001.4898	3001.5278	-12.6471	79	106	(R)HVDAHATLNDGVVVQVMGLLSNNNQALR(R)	1Met-ox

The matched peptides cover 29% (137/466AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.0 Mean Error = -31.9652 ppm Data Tolerance = 40.6713 ppm Mean Missed Cleavages = 0.1

5. 14/82 matches (17%). 70399.7 Da, pI = 5.76. Acc. # P34931. HUMAN. HEAT SHOCK 70 KDA PROTEIN 1-HOM (HSP70-HOM).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1112.5037 1112.5662 -56.1309 520 528 (R)MVLDAEKYK(A) 1Met-ox
1137.4957 1137.5540 -51.2554 527 535 (K)(E)
1197.6094 1197.6955 -71.9640 162 173 (K)(I)
1228.5511 1228.6286 -63.0424 28 38 (K)(T)
1232.5501 1232.6421 -74.6374 450 460 (R)(F)
1248.6170 1248.6370 -16.0878 450 460 (R)AMTKDNNLLGR(F) 1Met-ox
1287.5086 1287.6043 -74.3759 542 552 (K)(S)
1303.5173 1303.5993 -62.8862 542 552 (K)NALESYAFNMK(S) 1Met-ox
1465.7451 1465.8491 -70.9435 331 344 (K)(I)
1487.6335 1487.7018 -45.8937 39 51 (R)(L)
1675.6591 1675.7312 -43.0617 223 238 (K)(L)
2320.1365 2320.1495 -5.5922 364 386 (K)SINPDEAVAYGAAVQAAILMGDK(S) 1Met-ox
2786.3449 2786.3637 -6.7433 426 449 (K)(A)
2786.3449 2786.4399 -34.0690 538 563 (K)IAAKNALESYAFNMKSVVSDEGLKGK(I) 1Met-ox
3001.4898 3001.5610 -23.7071 79 104 (R)KFNDPVVQADMKLWPFQVINEGGKPK(V) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1112.5037	1112.5662	-56.1309	520	528	(R)MVLDAEKYK(A)	1Met-ox
1137.4957	1137.5540	-51.2554	527	535	(K)(E)
1197.6094	1197.6955	-71.9640	162	173	(K)(I)
1228.5511	1228.6286	-63.0424	28	38	(K)(T)
1232.5501	1232.6421	-74.6374	450	460	(R)(F)
1248.6170	1248.6370	-16.0878	450	460	(R)AMTKDNNLLGR(F)	1Met-ox
1287.5086	1287.6043	-74.3759	542	552	(K)(S)
1303.5173	1303.5993	-62.8862	542	552	(K)NALESYAFNMK(S)	1Met-ox
1465.7451	1465.8491	-70.9435	331	344	(K)(I)
1487.6335	1487.7018	-45.8937	39	51	(R)(L)
1675.6591	1675.7312	-43.0617	223	238	(K)(L)
2320.1365	2320.1495	-5.5922	364	386	(K)SINPDEAVAYGAAVQAAILMGDK(S)	1Met-ox
2786.3449	2786.3637	-6.7433	426	449	(K)(A)
2786.3449	2786.4399	-34.0690	538	563	(K)IAAKNALESYAFNMKSVVSDEGLKGK(I)	1Met-ox
3001.4898	3001.5610	-23.7071	79	104	(R)KFNDPVVQADMKLWPFQVINEGGKPK(V)	1Met-ox

The matched peptides cover 29% (192/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.1 Mean Error = -46.6927 ppm Data Tolerance = 48.8052 ppm Mean Missed Cleavages = 0.7