MS-Fit Search Results

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Sample ID (comment): D003
Database searched: SwissProt.7.25.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 1900 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |

4	150.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	5.65e+014	42/76 (55%)	53674.7 / 5.52	HUMAN	KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8)
2	1.97e+012	36/76 (47%)	54449.6 / 5.50	MOUSE	KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (CYTOKERATIN ENDO A)
3	2.22e+004	18/76 (23%)	89406.7 / 5.63	HUMAN	NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF-1) (AUTOANTIGEN NOR-90)
4	1.66e+004	17/76 (22%)	89509.7 / 5.59	MOUSE	NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF-1)
5	3.9e+003	9/76 (11%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)

Detailed Results

1. 42/76 matches (55%). 53674.7 Da, pI = 5.52. Acc. # P05787. HUMAN. KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
827.3248	827.4303	-127.5367	102	108	(K)(V)
870.3421	870.4321	-103.4471	33	40	(R)(V)
906.3467	906.4685	-134.3391	111	117	(R)(M)
1000.4399	1000.5679	-127.9272	317	325	(R)(G)
1000.4399	1000.4998	-59.9071	365	372	(K)QDMARQLR(E)	pyroGlu
1030.4423	1030.5686	-122.5562	123	130	(K)(T)
1060.4345	1060.5639	-121.9711	393	401	(R)(L)
1062.3933	1062.5108	-110.5240	226	233	(R)QLYEEEIR(E)	pyroGlu
1079.4097	1079.5373	-118.1710	226	233	(R)(E)
1079.4097	1079.5122	-94.8743	265	273	(K)(S)
1129.4984	1129.6217	-109.1315	342	352	(R)(L)
1129.4984	1129.6217	-109.1315	353	362	(K)(A)
1137.4575	1137.5792	-106.9612	286	295	(K)(H)
1153.4333	1153.5563	-106.6874	373	381	(R)(L)
1169.4175	1169.5512	-114.3341	276	285	(R)(Y)
1169.4175	1169.5512	-114.3341	373	381	(R)EYQELMNVK(L)	1Met-ox
1277.5904	1277.7105	-94.0208	382	392	(K)(K)
1320.5120	1320.6721	-121.2337	253	264	(R)(A)
1341.6094	1341.7490	-104.0926	317	328	(R)(A)
1344.5428	1344.6759	-98.9992	329	341	(R)(G)
1352.5357	1352.6772	-104.6194	187	197	(R)(K)
1368.5225	1368.6721	-109.3103	187	197	(R)TEMENEFVLIK(K)	1Met-ox
1412.5311	1412.6844	-108.4750	274	285	(R)(Y)
1419.6054	1419.7484	-100.6977	214	225	(R)(Q)
1428.5299	1428.6793	-104.5956	274	285	(R)SRAEAESMYQIK(Y)	1Met-ox
1442.6286	1442.8331	-141.7247	149	160	(R)(L)
1475.6035	1475.6987	-64.5137	402	414	(R)(T)
1480.6190	1480.7721	-103.4132	187	198	(R)(D)
1496.5992	1496.7671	-112.1236	187	198	(R)TEMENEFVLIKK(D)	1Met-ox
1566.5826	1566.7950	-135.5449	370	381	(R)QLREYQELMNVK(L)	1Met-ox
1792.6679	1792.8791	-117.8343	161	176	(K)(N)
1797.6237	1797.8329	-116.3405	199	213	(K)(L)
1808.6653	1808.8740	-115.3995	161	176	(K)LEAELGNMQGLVEDFK(N)	1Met-ox
1813.6128	1813.8278	-118.5511	199	213	(K)DVDEAYMNKVELESR(L)	1Met-ox
1847.6059	1847.8056	-108.0964	134	148	(R)(R)
1863.6172	1863.8006	-98.3940	134	148	(R)SNMDNMFESYINNLR(R)	1Met-ox
1879.6102	1879.7955	-98.5737	134	148	(R)SNMDNMFESYINNLR(R)	2Met-ox
1941.7765	1941.9228	-75.3070	198	213	(K)KDVDEAYMNKVELESR(L)	1Met-ox
1955.8315	1956.0402	-106.7040	329	347	(R)(D)
2003.6838	2003.9068	-111.2617	134	149	(R)(Q)
2019.7163	2019.9017	-91.7677	134	149	(R)SNMDNMFESYINNLRR(Q)	1Met-ox
2033.8471	2034.0582	-103.7569	159	176	(K)(N)
2035.7967	2035.8966	-49.0483	134	149	(R)SNMDNMFESYINNLRR(Q)	2Met-ox
2049.8750	2050.0531	-86.8701	159	176	(K)LKLEAELGNMQGLVEDFK(N)	1Met-ox
2108.8007	2109.0134	-100.8544	234	252	(R)(S)
2124.8362	2125.0083	-80.9714	234	252	(R)ELQSQISDTSVVLSMDNSR(S)	1Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 57% (278/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 55.3 Mean Error = -105.7593 ppm Data Tolerance = 36.8780 ppm Mean Missed Cleavages = 0.5

2. 36/76 matches (47%). 54449.6 Da, pI = 5.50. Acc. # P11679. MOUSE. KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (CYTOKERATIN ENDO A).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
827.3248 827.4303 -127.5367 108 114 (K)(V)
870.3421 870.4321 -103.4471 33 40 (R)(V)
906.3467 906.4685 -134.3391 117 123 (R)(M)
1000.4399 1000.4998 -59.9071 370 377 (K)QDMARQLR(E) pyroGlu
1030.4423 1030.5686 -122.5562 129 136 (K)(T)
1060.4345 1060.5639 -121.9711 398 406 (R)(L)
1079.4097 1079.5122 -94.8743 271 279 (R)(S)
1129.4984 1129.5312 -28.9924 9 18 (K)SYKMSTSGPR(A) 1Met-ox
1153.4333 1153.5563 -106.6874 378 386 (R)(L)
1169.4175 1169.5512 -114.3341 378 386 (R)EYQELMNVK(L) 1Met-ox
1307.5464 1307.7211 -133.5920 387 397 (K)(K)
1344.5428 1344.6759 -98.9992 334 346 (R)(G)
1352.5357 1352.6772 -104.6194 193 203 (R)(K)
1368.5225 1368.6721 -109.3103 193 203 (R)TEMENEFVLIK(K) 1Met-ox
1383.5472 1383.7272 -130.1471 103 114 (K)(V)
1419.6054 1419.7484 -100.6977 220 231 (R)(Q)
1442.6286 1442.6950 -45.9811 280 291 (R)SRAEAETMYQIK(Y) 1Met-ox
1475.6035 1475.6987 -64.5137 407 419 (R)(T)
1480.6190 1480.7721 -103.4132 193 204 (R)(D)
1496.5992 1496.7671 -112.1236 193 204 (R)TEMENEFVLIKK(D) 1Met-ox
1566.5826 1566.7950 -135.5449 375 386 (R)QLREYQELMNVK(L) 1Met-ox
1792.6679 1792.8791 -117.8343 167 182 (K)(N)
1797.6237 1797.8329 -116.3405 205 219 (K)(L)
1808.6653 1808.8740 -115.3995 167 182 (K)LEAELGNMQGLVEDFK(N) 1Met-ox
1813.6128 1813.8278 -118.5511 205 219 (K)DVDEAYMNKVELESR(L) 1Met-ox
1847.6059 1847.8056 -108.0964 140 154 (R)(R)
1863.6172 1863.8006 -98.3940 140 154 (R)SNMDNMFESYINNLR(R) 1Met-ox
1879.6102 1879.7955 -98.5737 140 154 (R)SNMDNMFESYINNLR(R) 2Met-ox
1941.7765 1941.9228 -75.3070 204 219 (K)KDVDEAYMNKVELESR(L) 1Met-ox
2003.6838 2003.9068 -111.2617 140 155 (R)(Q)
2019.7163 2019.9017 -91.7677 140 155 (R)SNMDNMFESYINNLRR(Q) 1Met-ox
2033.8471 2034.0582 -103.7569 165 182 (K)(N)
2035.7967 2035.8966 -49.0483 140 155 (R)SNMDNMFESYINNLRR(Q) 2Met-ox
2049.8750 2050.0531 -86.8701 165 182 (K)LKLEAELGNMQGLVEDFK(N) 1Met-ox
2108.8007 2109.0134 -100.8544 240 258 (R)(S)
2124.8362 2125.0083 -80.9714 240 258 (R)ELQSQISDTSVVLSMDNSR(S) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
827.3248	827.4303	-127.5367	108	114	(K)(V)
870.3421	870.4321	-103.4471	33	40	(R)(V)
906.3467	906.4685	-134.3391	117	123	(R)(M)
1000.4399	1000.4998	-59.9071	370	377	(K)QDMARQLR(E)	pyroGlu
1030.4423	1030.5686	-122.5562	129	136	(K)(T)
1060.4345	1060.5639	-121.9711	398	406	(R)(L)
1079.4097	1079.5122	-94.8743	271	279	(R)(S)
1129.4984	1129.5312	-28.9924	9	18	(K)SYKMSTSGPR(A)	1Met-ox
1153.4333	1153.5563	-106.6874	378	386	(R)(L)
1169.4175	1169.5512	-114.3341	378	386	(R)EYQELMNVK(L)	1Met-ox
1307.5464	1307.7211	-133.5920	387	397	(K)(K)
1344.5428	1344.6759	-98.9992	334	346	(R)(G)
1352.5357	1352.6772	-104.6194	193	203	(R)(K)
1368.5225	1368.6721	-109.3103	193	203	(R)TEMENEFVLIK(K)	1Met-ox
1383.5472	1383.7272	-130.1471	103	114	(K)(V)
1419.6054	1419.7484	-100.6977	220	231	(R)(Q)
1442.6286	1442.6950	-45.9811	280	291	(R)SRAEAETMYQIK(Y)	1Met-ox
1475.6035	1475.6987	-64.5137	407	419	(R)(T)
1480.6190	1480.7721	-103.4132	193	204	(R)(D)
1496.5992	1496.7671	-112.1236	193	204	(R)TEMENEFVLIKK(D)	1Met-ox
1566.5826	1566.7950	-135.5449	375	386	(R)QLREYQELMNVK(L)	1Met-ox
1792.6679	1792.8791	-117.8343	167	182	(K)(N)
1797.6237	1797.8329	-116.3405	205	219	(K)(L)
1808.6653	1808.8740	-115.3995	167	182	(K)LEAELGNMQGLVEDFK(N)	1Met-ox
1813.6128	1813.8278	-118.5511	205	219	(K)DVDEAYMNKVELESR(L)	1Met-ox
1847.6059	1847.8056	-108.0964	140	154	(R)(R)
1863.6172	1863.8006	-98.3940	140	154	(R)SNMDNMFESYINNLR(R)	1Met-ox
1879.6102	1879.7955	-98.5737	140	154	(R)SNMDNMFESYINNLR(R)	2Met-ox
1941.7765	1941.9228	-75.3070	204	219	(K)KDVDEAYMNKVELESR(L)	1Met-ox
2003.6838	2003.9068	-111.2617	140	155	(R)(Q)
2019.7163	2019.9017	-91.7677	140	155	(R)SNMDNMFESYINNLRR(Q)	1Met-ox
2033.8471	2034.0582	-103.7569	165	182	(K)(N)
2035.7967	2035.8966	-49.0483	140	155	(R)SNMDNMFESYINNLRR(Q)	2Met-ox
2049.8750	2050.0531	-86.8701	165	182	(K)LKLEAELGNMQGLVEDFK(N)	1Met-ox
2108.8007	2109.0134	-100.8544	240	258	(R)(S)
2124.8362	2125.0083	-80.9714	240	258	(R)ELQSQISDTSVVLSMDNSR(S)	1Met-ox

The matched peptides cover 45% (221/489AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 47.4 Mean Error = -100.7393 ppm Data Tolerance = 51.0233 ppm Mean Missed Cleavages = 0.5

3. 18/76 matches (23%). 89406.7 Da, pI = 5.63. Acc. # P17480. HUMAN. NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF-1) (AUTOANTIGEN NOR-90).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.4019 842.4484 -55.2075 661 668 (R)(T)
1062.3933 1062.5406 -138.6237 569 577 (K)(F)
1277.5904 1277.6425 -40.7536 597 606 (R)MVEIGSRWQR(I) 1Met-ox
1368.5225 1368.6694 -107.3485 540 549 (K)(A)
1419.6054 1419.7345 -90.8950 168 178 (R)(F)
1475.6035 1475.7681 -111.5372 440 451 (R)(K)
1797.6237 1797.8225 -110.5758 22 35 (R)(N)
1803.8241 1804.0234 -110.4545 70 83 (K)(T)
1813.6128 1813.8175 -112.8373 22 35 (R)WSQEDMLTLLECMK(N) 1Met-ox
1837.7398 1837.9482 -113.4092 347 360 (K)(F)
1863.6172 1863.7927 -94.1758 1 17 (-)(G)
1879.6102 1879.7876 -94.3914 1 17 (-)MNGEADCPTDLEMAAPK(G) 1Met-ox
1895.6187 1895.7825 -86.4326 1 17 (-)MNGEADCPTDLEMAAPK(G) 2Met-ox
1955.8315 1955.9393 -55.1258 509 524 (R)VKALKAMEMTWNNMEK(K) 2Met-ox
1993.7785 1993.9476 -84.7998 318 333 (K)DVPSTERMVLCSQQWK(L) 1Met-ox
2033.8471 2034.0409 -95.2577 487 503 (K)(F)
2035.7967 2036.0673 -132.8795 325 340 (R)MVLCSQQWKLLSQKEK(D) 1Met-ox
2051.8009 2052.0444 -118.6990 509 525 (R)(E)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.4019	842.4484	-55.2075	661	668	(R)(T)
1062.3933	1062.5406	-138.6237	569	577	(K)(F)
1277.5904	1277.6425	-40.7536	597	606	(R)MVEIGSRWQR(I)	1Met-ox
1368.5225	1368.6694	-107.3485	540	549	(K)(A)
1419.6054	1419.7345	-90.8950	168	178	(R)(F)
1475.6035	1475.7681	-111.5372	440	451	(R)(K)
1797.6237	1797.8225	-110.5758	22	35	(R)(N)
1803.8241	1804.0234	-110.4545	70	83	(K)(T)
1813.6128	1813.8175	-112.8373	22	35	(R)WSQEDMLTLLECMK(N)	1Met-ox
1837.7398	1837.9482	-113.4092	347	360	(K)(F)
1863.6172	1863.7927	-94.1758	1	17	(-)(G)
1879.6102	1879.7876	-94.3914	1	17	(-)MNGEADCPTDLEMAAPK(G)	1Met-ox
1895.6187	1895.7825	-86.4326	1	17	(-)MNGEADCPTDLEMAAPK(G)	2Met-ox
1955.8315	1955.9393	-55.1258	509	524	(R)VKALKAMEMTWNNMEK(K)	2Met-ox
1993.7785	1993.9476	-84.7998	318	333	(K)DVPSTERMVLCSQQWK(L)	1Met-ox
2033.8471	2034.0409	-95.2577	487	503	(K)(F)
2035.7967	2036.0673	-132.8795	325	340	(R)MVLCSQQWKLLSQKEK(D)	1Met-ox
2051.8009	2052.0444	-118.6990	509	525	(R)(E)

The matched peptides cover 23% (176/764AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.7 Mean Error = -97.4113 ppm Data Tolerance = 52.4030 ppm Mean Missed Cleavages = 1.2

4. 17/76 matches (22%). 89509.7 Da, pI = 5.59. Acc. # P25976. MOUSE. NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF-1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.4019 842.4484 -55.2075 661 668 (R)(T)
1062.3933 1062.5406 -138.6237 569 577 (K)(F)
1277.5904 1277.6425 -40.7536 597 606 (R)MVEIGSRWQR(I) 1Met-ox
1368.5225 1368.6694 -107.3485 540 549 (K)(A)
1419.6054 1419.7345 -90.8950 168 178 (R)(F)
1797.6237 1797.8225 -110.5758 22 35 (R)(N)
1803.8241 1804.0234 -110.4545 70 83 (K)(T)
1813.6128 1813.8175 -112.8373 22 35 (R)WSQEDMLTLLECMK(N) 1Met-ox
1837.7398 1837.9482 -113.4092 347 360 (K)(F)
1863.6172 1863.7927 -94.1758 1 17 (-)(G)
1879.6102 1879.7876 -94.3914 1 17 (-)MNGEADCPTDLEMAAPK(G) 1Met-ox
1895.6187 1895.7825 -86.4326 1 17 (-)MNGEADCPTDLEMAAPK(G) 2Met-ox
1955.8315 1955.9393 -55.1258 509 524 (R)VKALKAMEMTWNNMEK(K) 2Met-ox
1993.7785 1993.9476 -84.7998 318 333 (K)DVPSTERMVLCSQQWK(L) 1Met-ox
2033.8471 2034.0409 -95.2577 487 503 (K)(F)
2035.7967 2036.0673 -132.8795 325 340 (R)MVLCSQQWKLLSQKEK(D) 1Met-ox
2051.8009 2052.0444 -118.6990 509 525 (R)(E)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.4019	842.4484	-55.2075	661	668	(R)(T)
1062.3933	1062.5406	-138.6237	569	577	(K)(F)
1277.5904	1277.6425	-40.7536	597	606	(R)MVEIGSRWQR(I)	1Met-ox
1368.5225	1368.6694	-107.3485	540	549	(K)(A)
1419.6054	1419.7345	-90.8950	168	178	(R)(F)
1797.6237	1797.8225	-110.5758	22	35	(R)(N)
1803.8241	1804.0234	-110.4545	70	83	(K)(T)
1813.6128	1813.8175	-112.8373	22	35	(R)WSQEDMLTLLECMK(N)	1Met-ox
1837.7398	1837.9482	-113.4092	347	360	(K)(F)
1863.6172	1863.7927	-94.1758	1	17	(-)(G)
1879.6102	1879.7876	-94.3914	1	17	(-)MNGEADCPTDLEMAAPK(G)	1Met-ox
1895.6187	1895.7825	-86.4326	1	17	(-)MNGEADCPTDLEMAAPK(G)	2Met-ox
1955.8315	1955.9393	-55.1258	509	524	(R)VKALKAMEMTWNNMEK(K)	2Met-ox
1993.7785	1993.9476	-84.7998	318	333	(K)DVPSTERMVLCSQQWK(L)	1Met-ox
2033.8471	2034.0409	-95.2577	487	503	(K)(F)
2035.7967	2036.0673	-132.8795	325	340	(R)MVLCSQQWKLLSQKEK(D)	1Met-ox
2051.8009	2052.0444	-118.6990	509	525	(R)(E)

The matched peptides cover 21% (164/765AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.4 Mean Error = -96.5804 ppm Data Tolerance = 53.5246 ppm Mean Missed Cleavages = 1.2

5. 9/76 matches (11%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.4723 1179.6010 -109.0614 377 386 (K)(H)
1277.5904 1277.6337 -33.9202 247 257 (K)(N)
1277.5904 1277.7105 -94.0208 473 483 (K)(T)
1300.4276 1300.5302 -78.9198 258 267 (K)(N)
1383.5472 1383.6909 -103.8545 186 197 (K)(V)
1475.6035 1475.7858 -123.5691 200 211 (R)(W)
1475.6035 1475.7494 -98.9166 212 223 (K)(T)
1487.6318 1487.8043 -115.9417 240 251 (R)(L)
1941.7765 1941.9882 -108.9697 444 460 (K)(L)
1993.7785 1993.9772 -99.6652 224 239 (R)(R)
2383.6872 2383.9524 -111.2405 519 549 (R)(G)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.4723	1179.6010	-109.0614	377	386	(K)(H)
1277.5904	1277.6337	-33.9202	247	257	(K)(N)
1277.5904	1277.7105	-94.0208	473	483	(K)(T)
1300.4276	1300.5302	-78.9198	258	267	(K)(N)
1383.5472	1383.6909	-103.8545	186	197	(K)(V)
1475.6035	1475.7858	-123.5691	200	211	(R)(W)
1475.6035	1475.7494	-98.9166	212	223	(K)(T)
1487.6318	1487.8043	-115.9417	240	251	(R)(L)
1941.7765	1941.9882	-108.9697	444	460	(K)(L)
1993.7785	1993.9772	-99.6652	224	239	(R)(R)
2383.6872	2383.9524	-111.2405	519	549	(R)(G)

The matched peptides cover 23% (149/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.8 Mean Error = -98.0072 ppm Data Tolerance = 48.6526 ppm Mean Missed Cleavages = 0.5