MS-Fit Search Results

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Sample ID (comment): D5
Database searched: SwissProt.9.30.2001
Molecular weight search (1000 - 100000 Da) selects 94637 entries.
Full pI range: 100225 entries.
Species search ( HUMAN MOUSE ) selects 11762 entries.
Combined molecular weight, pI and species searches select 10474 entries.
Pre searches select 10474 entries.
MS-Fit search selects 958 entries (results displayed for top 10 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated |

4	100.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.38e+003	9/42 (21%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
2	1.92e+003	8/42 (19%)	89614.9 / 8.61	HUMAN	TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20)
3	429	7/42 (16%)	27745.3 / 4.73	HUMAN	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS)
4	415	6/42 (14%)	27764.5 / 4.68	HUMAN	14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA) (14-3-3 PROTEIN T-CELL) (HS1 PROTEIN)
5	414	6/42 (14%)	27778.5 / 4.69	MOUSE	14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA)
6	405	10/42 (23%)	69690.8 / 6.11	HUMAN	MERLIN (SCHWANNOMIN) (NEUROFIBROMIN 2)
7	396	8/42 (19%)	66350.5 / 5.74	HUMAN	GOLGIN-160
8	358	8/42 (19%)	69691.8 / 5.94	MOUSE	MERLIN (SCHWANNOMIN) (NEUROFIBROMIN 2)
9	321	7/42 (16%)	28374.7 / 4.66	HUMAN	14-3-3 PROTEIN GAMMA
10	298	8/42 (19%)	28219.0 / 4.76	HUMAN	14-3-3 PROTEIN ETA (PROTEIN AS1)

Detailed Results

1. 9/42 matches (21%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1127.5551	1127.5407	12.7828	289	298	(K)(V)
1179.6524	1179.6010	43.5902	377	386	(K)(H)
1277.7575	1277.6337	96.9084	247	257	(K)(N)
1277.7575	1277.7105	36.7999	473	483	(K)(T)
1383.7466	1383.6909	40.3142	186	197	(K)(V)
1475.8429	1475.7858	38.6597	200	211	(R)(W)
1475.8429	1475.7494	63.3163	212	223	(K)(T)
1638.9376	1638.8604	47.1343	186	199	(K)(F)
1657.9237	1657.7934	78.5923	13	29	(R)(R)
1994.0987	1993.9772	60.9169	224	239	(R)(R)
2384.1113	2383.9524	66.6511	519	549	(R)(G)

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The matched peptides cover 22% (144/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 21.4 Mean Error = 53.2424 ppm Data Tolerance = 46.0743 ppm Mean Missed Cleavages = 0.3

2. 8/42 matches (19%). 89614.9 Da, pI = 8.61. Acc. # P21580. HUMAN. TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
903.5179 903.4940 26.4875 142 148 (R)(S)
1179.6524 1179.5581 79.9704 677 685 (K)(F)
1261.6512 1261.5925 46.5360 355 364 (K)(R)
1493.7878 1493.6921 64.0481 622 632 (K)(E)
1791.8127 1791.8601 -26.4367 677 690 (K)(R)
1851.9771 1851.9751 1.1093 421 439 (K)(G)
1994.0987 1994.0500 24.4206 25 41 (R)(T)
2131.1446 2131.0090 63.6330 543 562 (R)(S)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
903.5179	903.4940	26.4875	142	148	(R)(S)
1179.6524	1179.5581	79.9704	677	685	(K)(F)
1261.6512	1261.5925	46.5360	355	364	(K)(R)
1493.7878	1493.6921	64.0481	622	632	(K)(E)
1791.8127	1791.8601	-26.4367	677	690	(K)(R)
1851.9771	1851.9751	1.1093	421	439	(K)(G)
1994.0987	1994.0500	24.4206	25	41	(R)(T)
2131.1446	2131.0090	63.6330	543	562	(R)(S)

The matched peptides cover 12% (98/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.0 Mean Error = 34.9710 ppm Data Tolerance = 71.4202 ppm Mean Missed Cleavages = 0.3

3. 7/42 matches (16%). 27745.3 Da, pI = 4.73. Acc. # P29312. HUMAN. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
816.3991 816.4216 -27.5470 12 18 (K)(Y)
1189.7011 1189.6615 33.2728 213 222 (K)(D)
1205.6919 1205.6564 29.4352 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1236.7060 1236.6523 43.4205 158 167 (K)(L)
1548.8151 1548.7142 65.1690 28 41 (K)(N)
2131.1446 2130.9622 85.5730 10 27 (K)AKLAEQAERYDDMAACMK(S) 1Met-ox
2718.2853 2718.4024 -43.0617 92 115 (R)(V)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
816.3991	816.4216	-27.5470	12	18	(K)(Y)
1189.7011	1189.6615	33.2728	213	222	(K)(D)
1205.6919	1205.6564	29.4352	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1236.7060	1236.6523	43.4205	158	167	(K)(L)
1548.8151	1548.7142	65.1690	28	41	(K)(N)
2131.1446	2130.9622	85.5730	10	27	(K)AKLAEQAERYDDMAACMK(S)	1Met-ox
2718.2853	2718.4024	-43.0617	92	115	(R)(V)

The matched peptides cover 31% (76/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 26.6088 ppm Data Tolerance = 93.4007 ppm Mean Missed Cleavages = 0.6

4. 6/42 matches (14%). 27764.5 Da, pI = 4.68. Acc. # P27348. HUMAN. 14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA) (14-3-3 PROTEIN T-CELL) (HS1 PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
816.3991 816.4216 -27.5470 12 18 (K)(Y)
903.5179 903.5151 3.1012 61 68 (R)(T)
1189.7011 1189.6615 33.2728 213 222 (K)(D)
1205.6919 1205.6564 29.4352 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1236.7060 1236.6523 43.4205 158 167 (K)(L)
1532.8110 1532.7193 59.8458 28 41 (K)(N)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
816.3991	816.4216	-27.5470	12	18	(K)(Y)
903.5179	903.5151	3.1012	61	68	(R)(T)
1189.7011	1189.6615	33.2728	213	222	(K)(D)
1205.6919	1205.6564	29.4352	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1236.7060	1236.6523	43.4205	158	167	(K)(L)
1532.8110	1532.7193	59.8458	28	41	(K)(N)

The matched peptides cover 20% (49/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.3 Mean Error = 23.5881 ppm Data Tolerance = 62.4208 ppm Mean Missed Cleavages = 0.2

5. 6/42 matches (14%). 27778.5 Da, pI = 4.69. Acc. # P35216. MOUSE. 14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
816.3991 816.4216 -27.5470 12 18 (K)(Y)
903.5179 903.5151 3.1012 61 68 (R)(T)
1189.7011 1189.6615 33.2728 213 222 (K)(D)
1205.6919 1205.6564 29.4352 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1236.7060 1236.6523 43.4205 158 167 (K)(L)
1532.8110 1532.7193 59.8458 28 41 (K)(N)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
816.3991	816.4216	-27.5470	12	18	(K)(Y)
903.5179	903.5151	3.1012	61	68	(R)(T)
1189.7011	1189.6615	33.2728	213	222	(K)(D)
1205.6919	1205.6564	29.4352	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1236.7060	1236.6523	43.4205	158	167	(K)(L)
1532.8110	1532.7193	59.8458	28	41	(K)(N)

The matched peptides cover 20% (49/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.3 Mean Error = 23.5881 ppm Data Tolerance = 62.4208 ppm Mean Missed Cleavages = 0.2

6. 10/42 matches (23%). 69690.8 Da, pI = 6.11. Acc. # P35240. HUMAN. MERLIN (SCHWANNOMIN) (NEUROFIBROMIN 2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1127.5551 1127.4931 55.0177 201 209 (R)(I)
1156.6746 1156.6438 26.5944 569 579 (R)(L)
1205.6919 1205.5901 84.3857 377 387 (R)(A)
1236.7060 1236.6411 52.5046 43 52 (K)(T)
1532.8110 1532.7789 20.9030 58 69 (R)(D)
1532.8110 1532.7305 52.5164 347 358 (R)(R)
1839.0112 1839.0016 5.1936 580 595 (K)(-)
2211.2047 2211.1450 27.0169 81 99 (K)(F)
2384.1113 2384.3230 -88.7758 270 289 (K)(L)
2718.2853 2718.4004 -42.3137 150 172 (K)(V)
2718.2853 2718.4170 -48.4338 424 446 (K)RLMEQKVLEAEVLALKMAEESER(R) 1Met-ox
2718.2853 2718.4170 -48.4338 425 447 (R)LMEQKVLEAEVLALKMAEESERR(A) 1Met-ox
3027.6911 3027.4120 92.1961 201 225 (R)DEAEMEYLKIAQDLEMYGVNYFAIR(N) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1127.5551	1127.4931	55.0177	201	209	(R)(I)
1156.6746	1156.6438	26.5944	569	579	(R)(L)
1205.6919	1205.5901	84.3857	377	387	(R)(A)
1236.7060	1236.6411	52.5046	43	52	(K)(T)
1532.8110	1532.7789	20.9030	58	69	(R)(D)
1532.8110	1532.7305	52.5164	347	358	(R)(R)
1839.0112	1839.0016	5.1936	580	595	(K)(-)
2211.2047	2211.1450	27.0169	81	99	(K)(F)
2384.1113	2384.3230	-88.7758	270	289	(K)(L)
2718.2853	2718.4004	-42.3137	150	172	(K)(V)
2718.2853	2718.4170	-48.4338	424	446	(K)RLMEQKVLEAEVLALKMAEESER(R)	1Met-ox
2718.2853	2718.4170	-48.4338	425	447	(R)LMEQKVLEAEVLALKMAEESERR(A)	1Met-ox
3027.6911	3027.4120	92.1961	201	225	(R)DEAEMEYLKIAQDLEMYGVNYFAIR(N)	1Met-ox

The matched peptides cover 30% (183/595AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.8 Mean Error = 14.4901 ppm Data Tolerance = 112.0975 ppm Mean Missed Cleavages = 1.6

7. 8/42 matches (19%). 66350.5 Da, pI = 5.74. Acc. # Q08378. HUMAN. GOLGIN-160.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
719.2862 719.3576 -99.2257 334 339 (R)(K)
804.3254 804.3892 -79.2642 522 527 (K)(E)
903.5179 903.5450 -29.9469 67 73 (K)(E)
1532.8110 1532.7630 31.2819 175 188 (K)(K)
1548.8151 1548.7579 36.9000 175 188 (K)EQMVAVTEANEALK(K) 1Met-ox
1638.9376 1638.8451 56.4438 52 66 (K)(K)
1994.0987 1993.9514 73.8588 144 160 (R)QHMADLEGHLQSAQKER(D) 1Met-ox
2212.2662 2212.1144 68.5879 116 134 (R)(L)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
719.2862	719.3576	-99.2257	334	339	(R)(K)
804.3254	804.3892	-79.2642	522	527	(K)(E)
903.5179	903.5450	-29.9469	67	73	(K)(E)
1532.8110	1532.7630	31.2819	175	188	(K)(K)
1548.8151	1548.7579	36.9000	175	188	(K)EQMVAVTEANEALK(K)	1Met-ox
1638.9376	1638.8451	56.4438	52	66	(K)(K)
1994.0987	1993.9514	73.8588	144	160	(R)QHMADLEGHLQSAQKER(D)	1Met-ox
2212.2662	2212.1144	68.5879	116	134	(R)(L)

The matched peptides cover 14% (84/579AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.0 Mean Error = 7.3295 ppm Data Tolerance = 135.8173 ppm Mean Missed Cleavages = 0.6

8. 8/42 matches (19%). 69691.8 Da, pI = 5.94. Acc. # P46662. MOUSE. MERLIN (SCHWANNOMIN) (NEUROFIBROMIN 2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1127.5551 1127.4931 55.0177 201 209 (R)(I)
1205.6919 1205.5901 84.3857 377 387 (R)(A)
1236.7060 1236.6411 52.5046 43 52 (K)(T)
1532.8110 1532.7789 20.9030 58 69 (R)(D)
1532.8110 1532.7305 52.5164 347 358 (R)(R)
1839.0112 1839.0016 5.1936 581 596 (K)(-)
2211.2047 2211.1450 27.0169 81 99 (K)(F)
2384.1113 2384.3230 -88.7758 270 289 (K)(L)
2718.2853 2718.4004 -42.3137 150 172 (K)(V)
2718.2853 2718.4170 -48.4338 424 446 (K)RLMEQKVLEAEVLALKMAEESER(R) 1Met-ox
2718.2853 2718.4170 -48.4338 425 447 (R)LMEQKVLEAEVLALKMAEESERR(A) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1127.5551	1127.4931	55.0177	201	209	(R)(I)
1205.6919	1205.5901	84.3857	377	387	(R)(A)
1236.7060	1236.6411	52.5046	43	52	(K)(T)
1532.8110	1532.7789	20.9030	58	69	(R)(D)
1532.8110	1532.7305	52.5164	347	358	(R)(R)
1839.0112	1839.0016	5.1936	581	596	(K)(-)
2211.2047	2211.1450	27.0169	81	99	(K)(F)
2384.1113	2384.3230	-88.7758	270	289	(K)(L)
2718.2853	2718.4004	-42.3137	150	172	(K)(V)
2718.2853	2718.4170	-48.4338	424	446	(K)RLMEQKVLEAEVLALKMAEESER(R)	1Met-ox
2718.2853	2718.4170	-48.4338	425	447	(R)LMEQKVLEAEVLALKMAEESERR(A)	1Met-ox

The matched peptides cover 26% (156/596AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.0 Mean Error = 6.3255 ppm Data Tolerance = 110.9586 ppm Mean Missed Cleavages = 1.6

9. 7/42 matches (16%). 28374.7 Da, pI = 4.66. Acc. # Q9UN99. HUMAN. 14-3-3 PROTEIN GAMMA.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
903.5179 903.5151 3.1012 62 69 (R)(T)
1189.7011 1189.6615 33.2728 216 225 (K)(D)
1205.6919 1205.6564 29.4352 216 225 (K)DSTLIMQLLR(D) 1Met-ox
1236.7060 1236.6588 38.1438 132 142 (R)(G)
1261.6512 1261.6112 31.7709 161 170 (K)EHMQPTHPIR(L) 1Met-ox
1643.8876 1643.7877 60.7809 29 42 (K)(N)
1994.0987 1993.9693 64.8603 125 141 (K)(R)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
903.5179	903.5151	3.1012	62	69	(R)(T)
1189.7011	1189.6615	33.2728	216	225	(K)(D)
1205.6919	1205.6564	29.4352	216	225	(K)DSTLIMQLLR(D)	1Met-ox
1236.7060	1236.6588	38.1438	132	142	(R)(G)
1261.6512	1261.6112	31.7709	161	170	(K)EHMQPTHPIR(L)	1Met-ox
1643.8876	1643.7877	60.7809	29	42	(K)(N)
1994.0987	1993.9693	64.8603	125	141	(K)(R)

The matched peptides cover 24% (60/247AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 37.3379 ppm Data Tolerance = 41.5518 ppm Mean Missed Cleavages = 0.4

10. 8/42 matches (19%). 28219.0 Da, pI = 4.76. Acc. # Q04917. HUMAN. 14-3-3 PROTEIN ETA (PROTEIN AS1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
816.3991 816.4216 -27.5470 13 19 (R)(Y)
903.5179 903.5151 3.1012 62 69 (R)(T)
1156.6746 1156.6075 58.0540 1 10 (-)GDREQLLQR(A) Acet N
1189.7011 1189.6615 33.2728 218 227 (K)(D)
1205.6919 1205.6564 29.4352 218 227 (K)DSTLIMQLLR(D) 1Met-ox
1236.7060 1236.6159 72.8452 163 172 (K)(L)
1261.6512 1261.6323 15.0227 1 10 (-)MGDREQLLQR(A) 1Met-ox
1383.7466 1383.7457 0.6886 1 12 (-)GDREQLLQRAR(L) Acet N

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
816.3991	816.4216	-27.5470	13	19	(R)(Y)
903.5179	903.5151	3.1012	62	69	(R)(T)
1156.6746	1156.6075	58.0540	1	10	(-)GDREQLLQR(A)	Acet N
1189.7011	1189.6615	33.2728	218	227	(K)(D)
1205.6919	1205.6564	29.4352	218	227	(K)DSTLIMQLLR(D)	1Met-ox
1236.7060	1236.6159	72.8452	163	172	(K)(L)
1261.6512	1261.6323	15.0227	1	10	(-)MGDREQLLQR(A)	1Met-ox
1383.7466	1383.7457	0.6886	1	12	(-)GDREQLLQRAR(L)	Acet N

The matched peptides cover 19% (47/246AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.0 Mean Error = 23.1091 ppm Data Tolerance = 64.9068 ppm Mean Missed Cleavages = 0.5