MS-Fit Search Results

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Sample ID (comment): D006
Database searched: SwissProt.7.25.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 1506 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	75.000 ppm	monoisotopic	Trypsin	3	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.95e+007	16/38 (42%)	50166.1 / 4.94	MOUSE	TUBULIN ALPHA-2 CHAIN
2	3.52e+007	16/38 (42%)	49924.9 / 4.95	HUMAN	TUBULIN ALPHA-4 CHAIN
3	1.52e+007	15/38 (39%)	50136.1 / 4.94	MOUSE	TUBULIN ALPHA-1 CHAIN
4	1.51e+007	16/38 (42%)	50158.1 / 5.02	HUMAN	TUBULIN ALPHA-1 CHAIN, BRAIN-SPECIFIC
5	1.17e+007	15/38 (39%)	49909.8 / 4.96	MOUSE	TUBULIN ALPHA-6 CHAIN

Detailed Results

1. 16/38 matches (42%). 50166.1 Da, pI = 4.94. Acc. # P05213. MOUSE. TUBULIN ALPHA-2 CHAIN.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1085.6425	1085.6206	20.1620	113	121	(K)(I)
1277.6518	1277.5845	52.7036	312	320	(K)YMACCLLYR(G)	2Cys-am
1293.6310	1293.5794	39.8674	312	320	(K)YMACCLLYR(G)	1Met-ox 2Cys-am
1410.8037	1410.7745	20.6832	85	96	(R)(E)
1487.9197	1487.8797	26.8617	230	243	(R)(F)
1612.8529	1612.7834	43.1175	340	352	(R)TIQFVDWCPTGFK(V)	1Cys-am
1701.9592	1701.9063	31.0942	65	79	(R)(T)
1718.9301	1718.8826	27.6229	216	229	(R)(L)
1756.9962	1756.9638	18.4574	265	280	(R)(A)
1878.9958	1878.9206	40.0175	374	390	(R)AVCMLSNTTAIAEAWAR(L)	1Cys-am
1894.9664	1894.9155	26.8340	374	390	(R)AVCMLSNTTAIAEAWAR(L)	1Met-ox 1Cys-am
2330.1128	2330.0188	40.3237	403	422	(R)(E)
2346.1472	2346.0137	56.8779	403	422	(R)AFVHWYVGEGMEEGEFSEAR(E)	1Met-ox
2409.2989	2409.2091	37.2846	244	264	(R)(I)
2764.3734	2764.3075	23.8704	281	304	(K)AYHEQLSVAEITNACFEPANQMVK(C)	1Cys-am
3405.6953	3405.5156	52.7736	402	430	(K)RAFVHWYVGEGMEEGEFSEAREDMAALEK(D)	2Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 42% (193/451AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 42.1 Mean Error = 34.9095 ppm Data Tolerance = 24.6653 ppm Mean Missed Cleavages = 0.1

2. 16/38 matches (42%). 49924.9 Da, pI = 4.95. Acc. # P05215. HUMAN. TUBULIN ALPHA-4 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1277.6518 1277.5845 52.7036 312 320 (K)YMACCLLYR(G) 2Cys-am
1293.6310 1293.5794 39.8674 312 320 (K)YMACCLLYR(G) 1Met-ox 2Cys-am
1410.8037 1410.7745 20.6832 85 96 (R)(E)
1487.9197 1487.8797 26.8617 230 243 (R)(F)
1598.8207 1598.7677 33.1263 340 352 (R)SIQFVDWCPTGFK(V) 1Cys-am
1715.9879 1715.9220 38.4034 65 79 (R)(N)
1718.9301 1718.8826 27.6229 216 229 (R)(L)
1754.9479 1754.8688 45.0320 339 352 (K)RSIQFVDWCPTGFK(V) 1Cys-am
1756.9962 1756.9638 18.4574 265 280 (R)(A)
1878.9958 1878.9206 40.0175 374 390 (R)AVCMLSNTTAIAEAWAR(L) 1Cys-am
1894.9664 1894.9155 26.8340 374 390 (R)AVCMLSNTTAIAEAWAR(L) 1Met-ox 1Cys-am
2330.1128 2330.0188 40.3237 403 422 (R)(E)
2346.1472 2346.0137 56.8779 403 422 (R)AFVHWYVGEGMEEGEFSEAR(E) 1Met-ox
2409.2989 2409.2091 37.2846 244 264 (R)(I)
2764.3734 2764.3075 23.8704 281 304 (K)AYHEQLSVAEITNACFEPANQMVK(C) 1Cys-am
3405.6953 3405.5156 52.7736 402 430 (K)RAFVHWYVGEGMEEGEFSEAREDMAALEK(D) 2Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1277.6518	1277.5845	52.7036	312	320	(K)YMACCLLYR(G)	2Cys-am
1293.6310	1293.5794	39.8674	312	320	(K)YMACCLLYR(G)	1Met-ox 2Cys-am
1410.8037	1410.7745	20.6832	85	96	(R)(E)
1487.9197	1487.8797	26.8617	230	243	(R)(F)
1598.8207	1598.7677	33.1263	340	352	(R)SIQFVDWCPTGFK(V)	1Cys-am
1715.9879	1715.9220	38.4034	65	79	(R)(N)
1718.9301	1718.8826	27.6229	216	229	(R)(L)
1754.9479	1754.8688	45.0320	339	352	(K)RSIQFVDWCPTGFK(V)	1Cys-am
1756.9962	1756.9638	18.4574	265	280	(R)(A)
1878.9958	1878.9206	40.0175	374	390	(R)AVCMLSNTTAIAEAWAR(L)	1Cys-am
1894.9664	1894.9155	26.8340	374	390	(R)AVCMLSNTTAIAEAWAR(L)	1Met-ox 1Cys-am
2330.1128	2330.0188	40.3237	403	422	(R)(E)
2346.1472	2346.0137	56.8779	403	422	(R)AFVHWYVGEGMEEGEFSEAR(E)	1Met-ox
2409.2989	2409.2091	37.2846	244	264	(R)(I)
2764.3734	2764.3075	23.8704	281	304	(K)AYHEQLSVAEITNACFEPANQMVK(C)	1Cys-am
3405.6953	3405.5156	52.7736	402	430	(K)RAFVHWYVGEGMEEGEFSEAREDMAALEK(D)	2Met-ox

The matched peptides cover 41% (185/448AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 42.1 Mean Error = 36.2962 ppm Data Tolerance = 23.4894 ppm Mean Missed Cleavages = 0.2

3. 15/38 matches (39%). 50136.1 Da, pI = 4.94. Acc. # P02551. MOUSE. TUBULIN ALPHA-1 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1085.6425 1085.6206 20.1620 113 121 (K)(I)
1277.6518 1277.5845 52.7036 312 320 (K)YMACCLLYR(G) 2Cys-am
1293.6310 1293.5794 39.8674 312 320 (K)YMACCLLYR(G) 1Met-ox 2Cys-am
1410.8037 1410.7745 20.6832 85 96 (R)(E)
1612.8529 1612.7834 43.1175 340 352 (R)TIQFVDWCPTGFK(V) 1Cys-am
1701.9592 1701.9063 31.0942 65 79 (R)(T)
1718.9301 1718.8826 27.6229 216 229 (R)(L)
1756.9962 1756.9638 18.4574 265 280 (R)(A)
1878.9958 1878.9206 40.0175 374 390 (R)AVCMLSNTTAIAEAWAR(L) 1Cys-am
1894.9664 1894.9155 26.8340 374 390 (R)AVCMLSNTTAIAEAWAR(L) 1Met-ox 1Cys-am
2330.1128 2330.0188 40.3237 403 422 (R)(E)
2346.1472 2346.0137 56.8779 403 422 (R)AFVHWYVGEGMEEGEFSEAR(E) 1Met-ox
2409.2989 2409.2091 37.2846 244 264 (R)(I)
2764.3734 2764.3075 23.8704 281 304 (K)AYHEQLSVAEITNACFEPANQMVK(C) 1Cys-am
3405.6953 3405.5156 52.7736 402 430 (K)RAFVHWYVGEGMEEGEFSEAREDMAALEK(D) 2Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 39% (179/451AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 39.5 Mean Error = 35.4460 ppm Data Tolerance = 25.1414 ppm Mean Missed Cleavages = 0.1

4. 16/38 matches (42%). 50158.1 Da, pI = 5.02. Acc. # P04687. HUMAN. TUBULIN ALPHA-1 CHAIN, BRAIN-SPECIFIC.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1085.6425 1085.6206 20.1620 113 121 (K)(I)
1277.6518 1277.5845 52.7036 312 320 (K)YMACCLLYR(G) 2Cys-am
1293.6310 1293.5794 39.8674 312 320 (K)YMACCLLYR(G) 1Met-ox 2Cys-am
1410.8037 1410.7745 20.6832 85 96 (R)(E)
1612.8529 1612.7834 43.1175 340 352 (R)TIQFVDWCPTGFK(V) 1Cys-am
1701.9592 1701.9063 31.0942 65 79 (R)(T)
1718.9301 1718.8826 27.6229 216 229 (R)(L)
1756.9962 1756.9638 18.4574 265 280 (R)(A)
1878.9958 1878.9206 40.0175 374 390 (R)AVCMLSNTTAIAEAWAR(L) 1Cys-am
1894.9664 1894.9155 26.8340 374 390 (R)AVCMLSNTTAIAEAWAR(L) 1Met-ox 1Cys-am
2330.1128 2330.0188 40.3237 403 422 (R)(E)
2346.1472 2346.0137 56.8779 403 422 (R)AFVHWYVGEGMEEGEFSEAR(E) 1Met-ox
2409.2989 2409.2091 37.2846 244 264 (R)(I)
2764.3734 2764.3075 23.8704 281 304 (K)AYHEQLSVAEITNACFEPANQMVK(C) 1Cys-am
3405.6953 3405.5156 52.7736 402 430 (K)RAFVHWYVGEGMEEGEFSEAREDMAALEK(D) 2Met-ox
3407.7702 3407.7098 17.7253 132 163 (R)(K)

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 46% (211/451AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 42.1 Mean Error = 34.3385 ppm Data Tolerance = 25.8546 ppm Mean Missed Cleavages = 0.2

5. 15/38 matches (39%). 49909.8 Da, pI = 4.96. Acc. # P05216. MOUSE. TUBULIN ALPHA-6 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1085.6425 1085.6206 20.1620 113 121 (K)(I)
1277.6518 1277.5845 52.7036 312 320 (K)YMACCLLYR(G) 2Cys-am
1293.6310 1293.5794 39.8674 312 320 (K)YMACCLLYR(G) 1Met-ox 2Cys-am
1410.8037 1410.7745 20.6832 85 96 (R)(E)
1487.9197 1487.8797 26.8617 230 243 (R)(F)
1612.8529 1612.7834 43.1175 340 352 (R)TIQFVDWCPTGFK(V) 1Cys-am
1701.9592 1701.9063 31.0942 65 79 (R)(T)
1718.9301 1718.8826 27.6229 216 229 (R)(L)
1756.9962 1756.9638 18.4574 265 280 (R)(A)
1878.9958 1878.9206 40.0175 374 390 (R)AVCMLSNTTAIAEAWAR(L) 1Cys-am
1894.9664 1894.9155 26.8340 374 390 (R)AVCMLSNTTAIAEAWAR(L) 1Met-ox 1Cys-am
2330.1128 2330.0188 40.3237 403 422 (R)(E)
2346.1472 2346.0137 56.8779 403 422 (R)AFVHWYVGEGMEEGEFSEAR(E) 1Met-ox
2409.2989 2409.2091 37.2846 244 264 (R)(I)
3405.6953 3405.5156 52.7736 402 430 (K)RAFVHWYVGEGMEEGEFSEAREDMAALEK(D) 2Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 37% (169/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 39.5 Mean Error = 35.6454 ppm Data Tolerance = 24.7930 ppm Mean Missed Cleavages = 0.1