MS-Fit Search Results

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Sample ID (comment): D008
Database searched: SwissProt.7.25.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 1750 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	125.000 ppm	monoisotopic	Trypsin	3	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.88e+009	24/61 (39%)	57116.8 / 4.76	HUMAN	PROTEIN DISULFIDE ISOMERASE PRECURSOR (PDI) (PROLYL 4-HYDROXYLASE BETA SUBUNIT) (CELLULAR THYROID HORMONE BINDING PROTEIN) (P55)
2	5.96e+006	17/61 (27%)	57144.1 / 4.80	MOUSE	PROTEIN DISULFIDE ISOMERASE PRECURSOR (PDI) (PROLYL 4-HYDROXYLASE BETA SUBUNIT) (CELLULAR THYROID HORMONE BINDING PROTEIN) (P55) (ERP59)
3	3.45e+003	10/61 (16%)	67255.0 / 9.06	MOUSE	ZINC FINGER PROTEIN 37 (ZFP-37) (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN)
4	2.37e+003	10/61 (16%)	94384.9 / 8.42	HUMAN	ZINC FINGER PROTEIN 33A (ZINC FINGER PROTEIN KOX31) (HA0946)
5	2.13e+003	9/61 (14%)	56621.8 / 5.99	MOUSE	PROTEIN DISULFIDE ISOMERASE A3 PRECURSOR (DISULFIDE ISOMERASE ER-60) (ERP60) (58 KDA MICROSOMAL PROTEIN) (P58) (ERP57)

Detailed Results

1. 24/61 matches (39%). 57116.8 Da, pI = 4.76. Acc. # P07237. HUMAN. PROTEIN DISULFIDE ISOMERASE PRECURSOR (PDI) (PROLYL 4-HYDROXYLASE BETA SUBUNIT) (CELLULAR THYROID HORMONE BINDING PROTEIN) (P55).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
910.3478	910.4423	-103.8204	445	452	(K)(T)
966.4588	966.5664	-111.3512	301	308	(R)(K)
1002.4222	1002.5042	-81.8328	309	316	(K)KEECPAVR(L)	1Cys-am
1066.4197	1066.5169	-91.1790	453	461	(R)(T)
1081.5752	1081.6774	-94.5067	255	263	(K)(S)
1202.4823	1202.6057	-102.6712	121	130	(R)(K)
1213.4183	1213.5377	-98.3834	376	385	(K)(K)
1341.5468	1341.6327	-64.0384	376	386	(K)(N)
1424.6611	1424.7789	-82.7386	196	207	(K)(K)
1451.5974	1451.7018	-71.9260	327	338	(K)(I)
1729.7801	1729.9125	-76.5199	410	424	(K)(M)
1780.6927	1780.8353	-80.1161	82	97	(K)(G)
1825.9349	1826.0944	-87.3269	248	263	(K)(S)
1833.7703	1833.9135	-78.1091	286	300	(K)(I)
1941.7731	1941.8780	-54.0246	387	401	(K)NVFVEFYAPWCGHCK(Q)	2Cys-am
1957.7361	1957.9408	-104.5387	445	461	(K)(T)
1964.9202	1965.0446	-63.2578	231	247	(K)(I)
1970.7751	1970.9297	-78.4353	43	57	(K)YLLVEFYAPWCGHCK(A)	2Cys-am
2009.8899	2010.0184	-63.9100	214	230	(R)(H)
2069.8232	2069.9730	-72.3518	386	401	(K)KNVFVEFYAPWCGHCK(Q)	2Cys-am
2418.1489	2418.2491	-41.4557	351	370	(K)(V)
2434.1238	2434.2441	-49.4157	351	370	(K)IKPHLMSQELPEDWDKQPVK(V)	1Met-ox
2713.1632	2713.3361	-63.7074	171	195	(K)(Y)
2935.3700	2935.4941	-42.2538	133	162	(R)(D)

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The matched peptides cover 54% (279/508AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 39.3 Mean Error = -77.4113 ppm Data Tolerance = 39.4865 ppm Mean Missed Cleavages = 0.4

2. 17/61 matches (27%). 57144.1 Da, pI = 4.80. Acc. # P09103. MOUSE. PROTEIN DISULFIDE ISOMERASE PRECURSOR (PDI) (PROLYL 4-HYDROXYLASE BETA SUBUNIT) (CELLULAR THYROID HORMONE BINDING PROTEIN) (P55) (ERP59).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
910.3478 910.4423 -103.8204 447 454 (K)(T)
966.4588 966.5664 -111.3512 303 310 (R)(K)
1002.4222 1002.5042 -81.8328 311 318 (K)KEECPAVR(L) 1Cys-am
1066.4197 1066.5169 -91.1790 455 463 (R)(T)
1081.5752 1081.6774 -94.5067 257 265 (K)(S)
1202.4823 1202.6057 -102.6712 123 132 (R)(K)
1424.6611 1424.7789 -82.7386 198 209 (K)(K)
1713.6829 1713.8911 -121.4736 17 32 (R)(K)
1780.6927 1780.8353 -80.1161 84 99 (K)(G)
1794.7412 1794.9278 -103.9749 373 388 (K)(N)
1825.9349 1826.0944 -87.3269 250 265 (K)(S)
1833.7703 1833.9135 -78.1091 288 302 (K)(I)
1941.7731 1941.8780 -54.0246 389 403 (K)NVFVEFYAPWCGHCK(Q) 2Cys-am
1957.7361 1957.9408 -104.5387 447 463 (K)(T)
1964.9202 1965.0446 -63.2578 233 249 (K)(I)
1970.7751 1970.9297 -78.4353 45 59 (K)YLLVEFYAPWCGHCK(A) 2Cys-am
2069.8232 2069.9730 -72.3518 388 403 (K)KNVFVEFYAPWCGHCK(Q) 2Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
910.3478	910.4423	-103.8204	447	454	(K)(T)
966.4588	966.5664	-111.3512	303	310	(R)(K)
1002.4222	1002.5042	-81.8328	311	318	(K)KEECPAVR(L)	1Cys-am
1066.4197	1066.5169	-91.1790	455	463	(R)(T)
1081.5752	1081.6774	-94.5067	257	265	(K)(S)
1202.4823	1202.6057	-102.6712	123	132	(R)(K)
1424.6611	1424.7789	-82.7386	198	209	(K)(K)
1713.6829	1713.8911	-121.4736	17	32	(R)(K)
1780.6927	1780.8353	-80.1161	84	99	(K)(G)
1794.7412	1794.9278	-103.9749	373	388	(K)(N)
1825.9349	1826.0944	-87.3269	250	265	(K)(S)
1833.7703	1833.9135	-78.1091	288	302	(K)(I)
1941.7731	1941.8780	-54.0246	389	403	(K)NVFVEFYAPWCGHCK(Q)	2Cys-am
1957.7361	1957.9408	-104.5387	447	463	(K)(T)
1964.9202	1965.0446	-63.2578	233	249	(K)(I)
1970.7751	1970.9297	-78.4353	45	59	(K)YLLVEFYAPWCGHCK(A)	2Cys-am
2069.8232	2069.9730	-72.3518	388	403	(K)KNVFVEFYAPWCGHCK(Q)	2Cys-am

The matched peptides cover 35% (181/509AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 27.9 Mean Error = -88.9240 ppm Data Tolerance = 35.4505 ppm Mean Missed Cleavages = 0.4

3. 10/61 matches (16%). 67255.0 Da, pI = 9.06. Acc. # P17141. MOUSE. ZINC FINGER PROTEIN 37 (ZFP-37) (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
966.4588 966.5121 -55.1481 268 275 (K)(T)
1002.4222 1002.5042 -81.8328 192 199 (K)CDKLPNNK(L) 1Cys-am
1780.6927 1780.7634 -39.7419 304 318 (R)THTGEKPYECEQCGK(A) 1Cys-am
1833.7703 1833.8264 -30.5844 416 430 (R)IHTGEKPFECNECGK(T) 2Cys-am
1837.7268 1837.8213 -51.3903 360 374 (R)SHTGEKPYECKECGK(S) 2Cys-am
1837.7268 1837.8577 -71.1870 444 458 (R)THTKEKPYKCDECGK(A) 1Cys-am
1851.8050 1851.8006 2.3892 304 318 (R)THTGEKPYECEQCGK(A) 2Cys-am
2085.8242 2085.9486 -59.6285 360 377 (R)(Y)
2383.7943 2384.0651 -113.5729 276 295 (R)THTGEKPYECNECGIAFSQK(S) 2Cys-am
2419.0847 2419.1182 -13.8231 341 359 (K)CNECGKTFRHSSNLMQHLR(S) 1Met-ox 2Cys-am
2713.1632 2713.3190 -57.4155 548 570 (K)SQLVIHQRSHTGEKPYECIECGK(A) 1Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
966.4588	966.5121	-55.1481	268	275	(K)(T)
1002.4222	1002.5042	-81.8328	192	199	(K)CDKLPNNK(L)	1Cys-am
1780.6927	1780.7634	-39.7419	304	318	(R)THTGEKPYECEQCGK(A)	1Cys-am
1833.7703	1833.8264	-30.5844	416	430	(R)IHTGEKPFECNECGK(T)	2Cys-am
1837.7268	1837.8213	-51.3903	360	374	(R)SHTGEKPYECKECGK(S)	2Cys-am
1837.7268	1837.8577	-71.1870	444	458	(R)THTKEKPYKCDECGK(A)	1Cys-am
1851.8050	1851.8006	2.3892	304	318	(R)THTGEKPYECEQCGK(A)	2Cys-am
2085.8242	2085.9486	-59.6285	360	377	(R)(Y)
2383.7943	2384.0651	-113.5729	276	295	(R)THTGEKPYECNECGIAFSQK(S)	2Cys-am
2419.0847	2419.1182	-13.8231	341	359	(K)CNECGKTFRHSSNLMQHLR(S)	1Met-ox 2Cys-am
2713.1632	2713.3190	-57.4155	548	570	(K)SQLVIHQRSHTGEKPYECIECGK(A)	1Cys-am

The matched peptides cover 23% (141/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.4 Mean Error = -51.9941 ppm Data Tolerance = 63.7863 ppm Mean Missed Cleavages = 0.8

4. 10/61 matches (16%). 94384.9 Da, pI = 8.42. Acc. # Q06730. HUMAN. ZINC FINGER PROTEIN 33A (ZINC FINGER PROTEIN KOX31) (HA0946).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
966.4588 966.5485 -92.7908 761 768 (K)(R)
1066.4197 1066.4086 10.3564 327 335 (K)(A)
1451.5974 1451.6048 -5.0706 324 335 (K)GEKHFECNECGK(A) 1Cys-am
1707.6476 1707.8302 -106.9097 181 194 (K)(N)
1707.6476 1707.7583 -64.8107 405 419 (R)THTGEKPYQCNACGK(T) 1Cys-am
1736.7053 1736.7372 -18.3800 377 391 (R)SHTGEKPFECNECGK(A) 1Cys-am
1736.7053 1736.7372 -18.3800 686 699 (K)HTGEKPYECNECGK(F) 2Cys-am
1736.7053 1736.7154 -5.8385 713 727 (R)AHTGEKSCQCNECGK(I) 2Cys-am
1765.6018 1765.8002 -112.3512 657 671 (R)(S)
1803.6234 1803.6880 -35.8107 239 253 (R)(T)
1837.7268 1837.7849 -31.5927 601 615 (R)THTGEKPYECNECGK(A) 2Cys-am
2383.7943 2384.0804 -119.9714 573 592 (R)(S)
2935.3700 2935.4056 -12.1036 287 312 (K)(L)
2935.3700 2935.4671 -33.0669 451 475 (R)VTSHLKVHQRTHTGEKPFECLECGK(S) 1Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
966.4588	966.5485	-92.7908	761	768	(K)(R)
1066.4197	1066.4086	10.3564	327	335	(K)(A)
1451.5974	1451.6048	-5.0706	324	335	(K)GEKHFECNECGK(A)	1Cys-am
1707.6476	1707.8302	-106.9097	181	194	(K)(N)
1707.6476	1707.7583	-64.8107	405	419	(R)THTGEKPYQCNACGK(T)	1Cys-am
1736.7053	1736.7372	-18.3800	377	391	(R)SHTGEKPFECNECGK(A)	1Cys-am
1736.7053	1736.7372	-18.3800	686	699	(K)HTGEKPYECNECGK(F)	2Cys-am
1736.7053	1736.7154	-5.8385	713	727	(R)AHTGEKSCQCNECGK(I)	2Cys-am
1765.6018	1765.8002	-112.3512	657	671	(R)(S)
1803.6234	1803.6880	-35.8107	239	253	(R)(T)
1837.7268	1837.7849	-31.5927	601	615	(R)THTGEKPYECNECGK(A)	2Cys-am
2383.7943	2384.0804	-119.9714	573	592	(R)(S)
2935.3700	2935.4056	-12.1036	287	312	(K)(L)
2935.3700	2935.4671	-33.0669	451	475	(R)VTSHLKVHQRTHTGEKPFECLECGK(S)	1Cys-am

The matched peptides cover 25% (209/810AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.4 Mean Error = -46.1943 ppm Data Tolerance = 89.0021 ppm Mean Missed Cleavages = 0.7

5. 9/61 matches (14%). 56621.8 Da, pI = 5.99. Acc. # P27773. MOUSE. PROTEIN DISULFIDE ISOMERASE A3 PRECURSOR (DISULFIDE ISOMERASE ER-60) (ERP60) (58 KDA MICROSOMAL PROTEIN) (P58) (ERP57).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
910.3478 910.4522 -114.6885 140 146 (R)(F)
1341.5468 1341.6843 -102.5261 448 459 (K)(K)
1780.6927 1780.8191 -70.9984 395 409 (K)(N)
1851.8050 1851.8562 -27.6696 395 409 (K)DVLIEFYAPWCGHCK(N) 1Cys-am
1970.7751 1970.9976 -112.8719 347 362 (K)(Y)
1979.8402 1979.9562 -58.5785 21 38 (R)(V)
2087.8821 2088.0514 -81.0716 465 481 (K)(E)
2293.8491 2294.0949 -107.1851 233 251 (K)KFIQDSIFGLCPHMTEDNK(D) 1Cys-am
2419.0847 2419.2482 -67.5867 173 193 (K)(E)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
910.3478	910.4522	-114.6885	140	146	(R)(F)
1341.5468	1341.6843	-102.5261	448	459	(K)(K)
1780.6927	1780.8191	-70.9984	395	409	(K)(N)
1851.8050	1851.8562	-27.6696	395	409	(K)DVLIEFYAPWCGHCK(N)	1Cys-am
1970.7751	1970.9976	-112.8719	347	362	(K)(Y)
1979.8402	1979.9562	-58.5785	21	38	(R)(V)
2087.8821	2088.0514	-81.0716	465	481	(K)(E)
2293.8491	2294.0949	-107.1851	233	251	(K)KFIQDSIFGLCPHMTEDNK(D)	1Cys-am
2419.0847	2419.2482	-67.5867	173	193	(K)(E)

The matched peptides cover 24% (125/504AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.8 Mean Error = -82.5752 ppm Data Tolerance = 58.7548 ppm Mean Missed Cleavages = 0.8