MS-Fit Search Results

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Sample ID (comment): D010
Database searched: SwissProt.7.25.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 2403 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	6.45e+005	14/80 (17%)	59296.8 / 4.34	HUMAN	PROTEIN KINASE C SUBSTRATE, 80 KD PROTEIN, HEAVY CHAIN (PKCSH) (80K-H PROTEIN)
2	3.65e+003	15/80 (18%)	59621.1 / 5.42	HUMAN	T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
3	1.23e+003	11/80 (13%)	80583.5 / 5.62	HUMAN	CALPAIN 11 (CALCIUM-ACTIVATED NEUTRAL PROTEINASE 11) (CANP 11)
4	1.02e+003	13/80 (16%)	63036.4 / 5.97	HUMAN	NUCLEAR RECEPTOR ROR-ALPHA (NUCLEAR RECEPTOR RZR-ALPHA)
5	1.01e+003	13/80 (16%)	58845.3 / 6.30	MOUSE	NUCLEAR RECEPTOR ROR-ALPHA (NUCLEAR RECEPTOR RZR-ALPHA)

Detailed Results

1. 14/80 matches (17%). 59296.8 Da, pI = 4.34. Acc. # P14314. HUMAN. PROTEIN KINASE C SUBSTRATE, 80 KD PROTEIN, HEAVY CHAIN (PKCSH) (80K-H PROTEIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
604.3378	604.3492	-18.9372	482	486	(R)LLCGK(E)	1Cys-am
715.4473	715.4255	30.4739	272	277	(R)(D)
1022.5182	1022.4907	26.8695	374	381	(R)(S)
1158.6369	1158.6159	18.1357	197	206	(K)(A)
1219.6209	1219.5993	17.7096	168	177	(K)(T)
1235.6118	1235.5942	14.2409	168	177	(K)SLEDQVEMLR(T)	1Met-ox
1291.6762	1291.6316	34.5059	125	135	(R)(E)
1307.6546	1307.6265	21.4819	125	135	(R)ESLQQMAEVTR(E)	1Met-ox
1315.7058	1315.6858	15.2208	178	188	(R)(E)
1315.7058	1315.6494	42.8772	181	191	(K)(E)
1448.7270	1448.7021	17.1495	207	219	(K)(E)
1566.6917	1566.6759	10.0426	464	476	(K)YEQGTGCWQGPNR(S)	1Cys-am
2181.0101	2180.9923	8.1913	355	373	(K)(N)
2197.0671	2196.9872	36.3983	355	373	(K)MPPYDEQTQAFIDAAQEAR(N)	1Met-ox
2211.0840	2211.0716	5.6055	374	391	(R)(N)

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The matched peptides cover 22% (119/527AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.5 Mean Error = 18.6644 ppm Data Tolerance = 30.0940 ppm Mean Missed Cleavages = 0.4

2. 15/80 matches (18%). 59621.1 Da, pI = 5.42. Acc. # P50990. HUMAN. T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
589.3252 589.3098 26.1531 319 322 (K)(R)
590.3232 590.3448 -36.6989 323 326 (R)RLCK(T) 1Cys-am
1158.6369 1158.5907 39.8453 467 476 (K)(N)
1169.5674 1169.5512 13.8476 261 270 (K)(G)
1307.6546 1307.6384 12.3925 21 31 (K)(N)
1365.6361 1365.5956 29.5984 379 390 (R)(A)
1378.6077 1378.6702 -45.2854 226 238 (K)(I)
1475.7824 1475.7640 12.4144 32 44 (R)(T)
1534.7619 1534.8229 -39.7755 391 404 (R)(D)
1625.8500 1625.8507 -0.4624 138 152 (R)(N)
1834.9536 1834.9737 -10.9253 207 224 (K)ILGSGISSSSVLHGMVFK(K) 1Met-ox
1851.8921 1851.9023 -5.5082 38 54 (K)(M)
1867.8744 1867.8972 -12.2449 38 54 (K)ELAQTTRTAYGPNGMNK(M) 1Met-ox
2220.1324 2220.1885 -25.2579 204 224 (R)VCKILGSGISSSSVLHGMVFK(K) 1Cys-am
2383.9986 2384.1114 -47.2909 261 281 (K)(A)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
589.3252	589.3098	26.1531	319	322	(K)(R)
590.3232	590.3448	-36.6989	323	326	(R)RLCK(T)	1Cys-am
1158.6369	1158.5907	39.8453	467	476	(K)(N)
1169.5674	1169.5512	13.8476	261	270	(K)(G)
1307.6546	1307.6384	12.3925	21	31	(K)(N)
1365.6361	1365.5956	29.5984	379	390	(R)(A)
1378.6077	1378.6702	-45.2854	226	238	(K)(I)
1475.7824	1475.7640	12.4144	32	44	(R)(T)
1534.7619	1534.8229	-39.7755	391	404	(R)(D)
1625.8500	1625.8507	-0.4624	138	152	(R)(N)
1834.9536	1834.9737	-10.9253	207	224	(K)ILGSGISSSSVLHGMVFK(K)	1Met-ox
1851.8921	1851.9023	-5.5082	38	54	(K)(M)
1867.8744	1867.8972	-12.2449	38	54	(K)ELAQTTRTAYGPNGMNK(M)	1Met-ox
2220.1324	2220.1885	-25.2579	204	224	(R)VCKILGSGISSSSVLHGMVFK(K)	1Cys-am
2383.9986	2384.1114	-47.2909	261	281	(K)(A)

The matched peptides cover 27% (148/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.8 Mean Error = -5.9465 ppm Data Tolerance = 56.7467 ppm Mean Missed Cleavages = 0.6

3. 11/80 matches (13%). 80583.5 Da, pI = 5.62. Acc. # Q9UMQ6. HUMAN. CALPAIN 11 (CALCIUM-ACTIVATED NEUTRAL PROTEINASE 11) (CANP 11).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
515.3366 515.3306 11.6893 557 560 (R)(M)
529.3821 529.3826 -0.8797 224 227 (R)(A)
604.3378 604.3241 22.6823 34 38 (R)AACLR(K) 1Cys-am
1099.5945 1099.6338 -35.7422 561 569 (R)(T)
1263.6992 1263.6949 3.4325 588 599 (K)(I)
1478.7181 1478.7064 7.9198 672 683 (K)(N)
1510.7655 1510.6962 45.8496 672 683 (K)TMFTFFLTMDPK(N) 2Met-ox
2157.9726 2158.0281 -25.7256 570 587 (K)TKGFGLDACRCMINLMDK(D) 2Cys-am
2283.1470 2283.1551 -3.5640 580 599 (R)CMINLMDKDGSGKLGLLEFK(I) 1Cys-am
2463.2140 2463.0952 48.2309 506 525 (K)(V)
2733.2683 2733.1496 43.4554 608 629 (K)(L)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
515.3366	515.3306	11.6893	557	560	(R)(M)
529.3821	529.3826	-0.8797	224	227	(R)(A)
604.3378	604.3241	22.6823	34	38	(R)AACLR(K)	1Cys-am
1099.5945	1099.6338	-35.7422	561	569	(R)(T)
1263.6992	1263.6949	3.4325	588	599	(K)(I)
1478.7181	1478.7064	7.9198	672	683	(K)(N)
1510.7655	1510.6962	45.8496	672	683	(K)TMFTFFLTMDPK(N)	2Met-ox
2157.9726	2158.0281	-25.7256	570	587	(K)TKGFGLDACRCMINLMDK(D)	2Cys-am
2283.1470	2283.1551	-3.5640	580	599	(R)CMINLMDKDGSGKLGLLEFK(I)	1Cys-am
2463.2140	2463.0952	48.2309	506	525	(K)(V)
2733.2683	2733.1496	43.4554	608	629	(K)(L)

The matched peptides cover 15% (106/702AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.8 Mean Error = 10.6680 ppm Data Tolerance = 55.6537 ppm Mean Missed Cleavages = 0.8

4. 13/80 matches (16%). 63036.4 Da, pI = 5.97. Acc. # P35398. HUMAN. NUCLEAR RECEPTOR ROR-ALPHA (NUCLEAR RECEPTOR RZR-ALPHA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
590.3232 590.3084 24.9370 512 516 (R)ALCGR(H) 1Cys-am
804.3759 804.4038 -34.6697 147 152 (K)NCLIDR(T) 1Cys-am
1153.5904 1153.6264 -31.1848 178 186 (K)FGRMSKKQR(D) 1Met-ox
1245.6347 1245.5898 36.0911 33 45 (R)(R)
1291.6762 1291.6543 16.9699 350 359 (R)EVMWQLCAIK(I) 1Cys-am
1307.6546 1307.6492 4.1606 350 359 (R)EVMWQLCAIK(I) 1Met-ox 1Cys-am
1504.7945 1504.7768 11.6964 348 359 (K)(I)
1510.7655 1510.8157 -33.2646 360 372 (K)(R)
1520.7541 1520.7718 -11.6429 348 359 (K)QREVMWQLCAIK(I) 1Met-ox
1625.8500 1625.8256 15.0080 166 180 (K)CLAVGMSRDAVKFGR(M) 1Met-ox
1707.7719 1707.7132 34.3615 1 16 (-)MNEGAPGDSDLETEAR(V) 1Met-ox
2283.1470 2283.0572 39.3270 108 127 (K)ICGDKSSGIHYGVITCEGCK(G) 3Cys-am
2733.2683 2733.3064 -13.9045 108 132 (K)(S)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
590.3232	590.3084	24.9370	512	516	(R)ALCGR(H)	1Cys-am
804.3759	804.4038	-34.6697	147	152	(K)NCLIDR(T)	1Cys-am
1153.5904	1153.6264	-31.1848	178	186	(K)FGRMSKKQR(D)	1Met-ox
1245.6347	1245.5898	36.0911	33	45	(R)(R)
1291.6762	1291.6543	16.9699	350	359	(R)EVMWQLCAIK(I)	1Cys-am
1307.6546	1307.6492	4.1606	350	359	(R)EVMWQLCAIK(I)	1Met-ox 1Cys-am
1504.7945	1504.7768	11.6964	348	359	(K)(I)
1510.7655	1510.8157	-33.2646	360	372	(K)(R)
1520.7541	1520.7718	-11.6429	348	359	(K)QREVMWQLCAIK(I)	1Met-ox
1625.8500	1625.8256	15.0080	166	180	(K)CLAVGMSRDAVKFGR(M)	1Met-ox
1707.7719	1707.7132	34.3615	1	16	(-)MNEGAPGDSDLETEAR(V)	1Met-ox
2283.1470	2283.0572	39.3270	108	127	(K)ICGDKSSGIHYGVITCEGCK(G)	3Cys-am
2733.2683	2733.3064	-13.9045	108	132	(K)(S)

The matched peptides cover 19% (111/556AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.3 Mean Error = 4.4527 ppm Data Tolerance = 53.7626 ppm Mean Missed Cleavages = 0.8

5. 13/80 matches (16%). 58845.3 Da, pI = 6.30. Acc. # P51448. MOUSE. NUCLEAR RECEPTOR ROR-ALPHA (NUCLEAR RECEPTOR RZR-ALPHA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
590.3232 590.3084 24.9370 479 483 (R)ALCGR(H) 1Cys-am
604.3378 604.3670 -48.3204 56 61 (R)(K)
804.3759 804.4038 -34.6697 114 119 (K)NCLIDR(T) 1Cys-am
1153.5904 1153.6264 -31.1848 145 153 (K)FGRMSKKQR(D) 1Met-ox
1245.6347 1245.6439 -7.3772 28 38 (R)(S)
1291.6762 1291.6543 16.9699 317 326 (R)EVMWQLCAIK(I) 1Cys-am
1307.6546 1307.6492 4.1606 317 326 (R)EVMWQLCAIK(I) 1Met-ox 1Cys-am
1504.7945 1504.7768 11.6964 315 326 (K)(I)
1510.7655 1510.8157 -33.2646 327 339 (K)(R)
1520.7541 1520.7718 -11.6429 315 326 (K)QREVMWQLCAIK(I) 1Met-ox
1625.8500 1625.8256 15.0080 133 147 (K)CLAVGMSRDAVKFGR(M) 1Met-ox
2283.1470 2283.0572 39.3270 75 94 (K)ICGDKSSGIHYGVITCEGCK(G) 3Cys-am
2733.2683 2733.3064 -13.9045 75 99 (K)(S)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
590.3232	590.3084	24.9370	479	483	(R)ALCGR(H)	1Cys-am
604.3378	604.3670	-48.3204	56	61	(R)(K)
804.3759	804.4038	-34.6697	114	119	(K)NCLIDR(T)	1Cys-am
1153.5904	1153.6264	-31.1848	145	153	(K)FGRMSKKQR(D)	1Met-ox
1245.6347	1245.6439	-7.3772	28	38	(R)(S)
1291.6762	1291.6543	16.9699	317	326	(R)EVMWQLCAIK(I)	1Cys-am
1307.6546	1307.6492	4.1606	317	326	(R)EVMWQLCAIK(I)	1Met-ox 1Cys-am
1504.7945	1504.7768	11.6964	315	326	(K)(I)
1510.7655	1510.8157	-33.2646	327	339	(K)(R)
1520.7541	1520.7718	-11.6429	315	326	(K)QREVMWQLCAIK(I)	1Met-ox
1625.8500	1625.8256	15.0080	133	147	(K)CLAVGMSRDAVKFGR(M)	1Met-ox
2283.1470	2283.0572	39.3270	75	94	(K)ICGDKSSGIHYGVITCEGCK(G)	3Cys-am
2733.2683	2733.3064	-13.9045	75	99	(K)(S)

The matched peptides cover 18% (99/523AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.3 Mean Error = -5.2512 ppm Data Tolerance = 53.1555 ppm Mean Missed Cleavages = 0.9