MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): D12
Database searched: SwissProt.9.30.2001
Molecular weight search (1000 - 100000 Da) selects 94637 entries.
Full pI range: 100225 entries.
Species search ( HUMAN MOUSE ) selects 11762 entries.
Combined molecular weight, pI and species searches select 10474 entries.
Pre searches select 10474 entries.
MS-Fit search selects 780 entries (results displayed for top 10 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated |

4	50.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	7.67e+003	15/46 (32%)	51335.1 / 5.41	HUMAN	KERATIN, TYPE II CYTOSKELETAL 7 (CYTOKERATIN 7) (K7) (CK 7)
2	6.72e+003	9/46 (19%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
3	2.91e+003	9/46 (19%)	53674.7 / 5.52	HUMAN	KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8)
4	2.65e+003	8/46 (17%)	54449.6 / 5.50	MOUSE	KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (CYTOKERATIN ENDO A)
5	229	7/46 (15%)	59519.1 / 5.13	HUMAN	KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (K10) (CK 10)
6	206	14/46 (30%)	89406.7 / 5.63	HUMAN	NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF-1) (AUTOANTIGEN NOR-90)
7	141	7/46 (15%)	41737.1 / 5.29	HUMAN	ACTIN, CYTOPLASMIC 1 (BETA-ACTIN)
8	141	7/46 (15%)	41793.2 / 5.31	HUMAN	ACTIN, CYTOPLASMIC 2 (GAMMA-ACTIN)
9	130	6/46 (13%)	70424.7 / 8.51	MOUSE	PROTEIN TRANSPORT PROTEIN SEC23A (SEC23-RELATED PROTEIN A)
10	121	4/46 (8%)	30658.9 / 8.84	HUMAN	VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL PROTEIN 3 (VDAC-3) (HVDAC3) (OUTER MITOCHONDRIAL MEMBRANE PROTEIN PORIN 3)

Detailed Results

1. 15/46 matches (32%). 51335.1 Da, pI = 5.41. Acc. # P08729. HUMAN. KERATIN, TYPE II CYTOSKELETAL 7 (CYTOKERATIN 7) (K7) (CK 7).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1014.4946	1014.5220	-27.0337	78	86	(R)(V)
1104.5733	1104.5802	-6.2611	53	64	(R)(E)
1242.6556	1242.6442	9.1718	150	161	(R)(L)
1277.7163	1277.7105	4.5297	383	393	(K)(K)
1385.7553	1385.7389	11.8483	352	363	(R)(A)
1385.7553	1385.7389	11.8483	354	365	(K)(Q)
1405.7620	1405.8055	-30.9363	383	394	(K)(L)
1406.7296	1406.7068	16.1917	1	13	(-)SIHFSSPVFTSR(S)	Acet N
1418.7427	1418.7280	10.3922	215	226	(K)(T)
1442.8237	1442.8007	15.9126	137	149	(R)(G)
1453.8630	1453.8379	17.2707	65	77	(R)(L)
1812.9699	1812.9053	35.5985	199	214	(K)KDVDAAYMSKVELEAK(V)	1Met-ox
1847.8582	1847.9146	-30.5034	303	317	(R)NTRNEISEMNRAIQR(L)	1Met-ox
2109.0981	2109.1893	-43.2117	314	331	(R)(L)
3010.5281	3010.4527	25.0470	227	253	(R)(S)
3026.5748	3026.4476	42.0219	227	253	(R)TLNETELTELQSQISDTSVVLSMDNSR(S)	1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 38% (182/469AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 32.6 Mean Error = 3.8679 ppm Data Tolerance = 49.6734 ppm Mean Missed Cleavages = 0.6

2. 9/46 matches (19%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1014.4946 1014.4791 15.2634 1 8 (-)MSRQFSSR(S) 1Met-ox
1179.5873 1179.6010 -11.5878 377 386 (K)(H)
1277.7163 1277.7105 4.5297 473 483 (K)(T)
1383.7092 1383.6909 13.2583 186 197 (K)(V)
1475.7745 1475.7858 -7.6729 200 211 (R)(W)
1475.7745 1475.7494 16.9825 212 223 (K)(T)
1657.8422 1657.7934 29.4451 13 29 (R)(R)
1716.8887 1716.8517 21.5480 418 432 (K)(G)
1954.0752 1954.0106 33.0359 387 403 (R)(V)
1994.0540 1993.9772 38.5079 224 239 (R)(R)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1014.4946	1014.4791	15.2634	1	8	(-)MSRQFSSR(S)	1Met-ox
1179.5873	1179.6010	-11.5878	377	386	(K)(H)
1277.7163	1277.7105	4.5297	473	483	(K)(T)
1383.7092	1383.6909	13.2583	186	197	(K)(V)
1475.7745	1475.7858	-7.6729	200	211	(R)(W)
1475.7745	1475.7494	16.9825	212	223	(K)(T)
1657.8422	1657.7934	29.4451	13	29	(R)(R)
1716.8887	1716.8517	21.5480	418	432	(K)(G)
1954.0752	1954.0106	33.0359	387	403	(R)(V)
1994.0540	1993.9772	38.5079	224	239	(R)(R)

The matched peptides cover 20% (130/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.6 Mean Error = 15.3310 ppm Data Tolerance = 33.1058 ppm Mean Missed Cleavages = 0.3

3. 9/46 matches (19%). 53674.7 Da, pI = 5.52. Acc. # P05787. HUMAN. KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1079.5357 1079.5373 -1.4590 226 233 (R)(E)
1079.5357 1079.5122 21.8405 265 273 (K)(S)
1129.6411 1129.6217 17.1691 342 352 (R)(L)
1129.6411 1129.6217 17.1691 353 362 (K)(A)
1277.7163 1277.7105 4.5297 382 392 (K)(K)
1344.6995 1344.6759 17.5114 329 341 (R)(G)
1405.7620 1405.8055 -30.9363 382 393 (K)(L)
1419.7527 1419.7484 3.0305 214 225 (R)(Q)
1442.8237 1442.8331 -6.5178 149 160 (R)(L)
1442.8237 1442.8695 -31.7351 460 472 (R)(L)
1847.8582 1847.8056 28.4694 134 148 (R)(R)
2109.0981 2109.0134 40.1751 234 252 (R)(S)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1079.5357	1079.5373	-1.4590	226	233	(R)(E)
1079.5357	1079.5122	21.8405	265	273	(K)(S)
1129.6411	1129.6217	17.1691	342	352	(R)(L)
1129.6411	1129.6217	17.1691	353	362	(K)(A)
1277.7163	1277.7105	4.5297	382	392	(K)(K)
1344.6995	1344.6759	17.5114	329	341	(R)(G)
1405.7620	1405.8055	-30.9363	382	393	(K)(L)
1419.7527	1419.7484	3.0305	214	225	(R)(Q)
1442.8237	1442.8331	-6.5178	149	160	(R)(L)
1442.8237	1442.8695	-31.7351	460	472	(R)(L)
1847.8582	1847.8056	28.4694	134	148	(R)(R)
2109.0981	2109.0134	40.1751	234	252	(R)(S)

The matched peptides cover 27% (134/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.6 Mean Error = 6.6039 ppm Data Tolerance = 43.9541 ppm Mean Missed Cleavages = 0.6

4. 8/46 matches (17%). 54449.6 Da, pI = 5.50. Acc. # P11679. MOUSE. KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (CYTOKERATIN ENDO A).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1079.5357 1079.5122 21.8405 271 279 (R)(S)
1307.6806 1307.7211 -30.9348 387 397 (K)(K)
1344.6995 1344.6759 17.5114 334 346 (R)(G)
1383.7092 1383.7272 -13.0373 103 114 (K)(V)
1419.7527 1419.7484 3.0305 220 231 (R)(Q)
1442.8237 1442.8695 -31.7351 466 478 (K)(L)
1847.8582 1847.8056 28.4694 140 154 (R)(R)
2109.0981 2109.0134 40.1751 240 258 (R)(S)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1079.5357	1079.5122	21.8405	271	279	(R)(S)
1307.6806	1307.7211	-30.9348	387	397	(K)(K)
1344.6995	1344.6759	17.5114	334	346	(R)(G)
1383.7092	1383.7272	-13.0373	103	114	(K)(V)
1419.7527	1419.7484	3.0305	220	231	(R)(Q)
1442.8237	1442.8695	-31.7351	466	478	(K)(L)
1847.8582	1847.8056	28.4694	140	154	(R)(R)
2109.0981	2109.0134	40.1751	240	258	(R)(S)

The matched peptides cover 21% (104/489AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.4 Mean Error = 4.4150 ppm Data Tolerance = 54.4872 ppm Mean Missed Cleavages = 0.5

5. 7/46 matches (15%). 59519.1 Da, pI = 5.13. Acc. # P13645. HUMAN. KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (K10) (CK 10).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1090.5042 1090.5315 -25.0063 148 156 (K)(L)
1109.4866 1109.4904 -3.4050 335 343 (K)(S)
1234.6736 1234.6796 -4.8430 236 245 (R)(Q)
1300.6081 1300.5956 9.5979 286 295 (K)(N)
1434.7881 1434.7705 12.2453 440 450 (K)(S)
1707.8188 1707.7727 26.9962 41 59 (K)(G)
1997.0057 1996.9715 17.1286 346 362 (K)(S)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1090.5042	1090.5315	-25.0063	148	156	(K)(L)
1109.4866	1109.4904	-3.4050	335	343	(K)(S)
1234.6736	1234.6796	-4.8430	236	245	(R)(Q)
1300.6081	1300.5956	9.5979	286	295	(K)(N)
1434.7881	1434.7705	12.2453	440	450	(K)(S)
1707.8188	1707.7727	26.9962	41	59	(K)(G)
1997.0057	1996.9715	17.1286	346	362	(K)(S)

The matched peptides cover 14% (85/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.2 Mean Error = 4.6734 ppm Data Tolerance = 34.3679 ppm Mean Missed Cleavages = 0.4

6. 14/46 matches (30%). 89406.7 Da, pI = 5.63. Acc. # P17480. HUMAN. NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF-1) (AUTOANTIGEN NOR-90).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.4863 842.4484 44.9518 661 668 (R)(T)
1062.5231 1062.5406 -16.4495 569 577 (K)(F)
1242.6556 1242.6555 0.1319 470 480 (R)(L)
1418.7427 1418.7619 -13.4902 122 132 (R)(L)
1419.7527 1419.7345 12.8343 168 178 (R)(F)
1434.7881 1434.7568 21.8190 122 132 (R)FFMEKRAKYAK(L) 1Met-ox
1434.7881 1434.7164 49.9842 341 351 (K)(K)
1475.7745 1475.7681 4.3604 440 451 (R)(K)
1515.7867 1515.8569 -46.3039 373 385 (R)(Q)
1731.9476 1731.9580 -5.9753 440 453 (R)(A)
1744.8629 1744.8580 2.8270 254 266 (R)(H)
1847.8582 1847.9503 -49.8215 225 240 (K)(K)
1954.0752 1954.0582 8.6658 458 475 (K)(E)
1997.0057 1997.0555 -24.9362 267 284 (K)(A)
2873.5120 2873.4719 13.9436 361 384 (R)(K)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.4863	842.4484	44.9518	661	668	(R)(T)
1062.5231	1062.5406	-16.4495	569	577	(K)(F)
1242.6556	1242.6555	0.1319	470	480	(R)(L)
1418.7427	1418.7619	-13.4902	122	132	(R)(L)
1419.7527	1419.7345	12.8343	168	178	(R)(F)
1434.7881	1434.7568	21.8190	122	132	(R)FFMEKRAKYAK(L)	1Met-ox
1434.7881	1434.7164	49.9842	341	351	(K)(K)
1475.7745	1475.7681	4.3604	440	451	(R)(K)
1515.7867	1515.8569	-46.3039	373	385	(R)(Q)
1731.9476	1731.9580	-5.9753	440	453	(R)(A)
1744.8629	1744.8580	2.8270	254	266	(R)(H)
1847.8582	1847.9503	-49.8215	225	240	(K)(K)
1954.0752	1954.0582	8.6658	458	475	(K)(E)
1997.0057	1997.0555	-24.9362	267	284	(K)(A)
2873.5120	2873.4719	13.9436	361	384	(R)(K)

The matched peptides cover 20% (159/764AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 30.4 Mean Error = 0.1694 ppm Data Tolerance = 56.5491 ppm Mean Missed Cleavages = 2.0

7. 7/46 matches (15%). 41737.1 Da, pI = 5.29. Acc. # P02570. HUMAN. ACTIN, CYTOPLASMIC 1 (BETA-ACTIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1014.4946 1014.4818 12.6163 184 191 (R)DLTDYLMK(I) 1Met-ox
1129.6411 1129.6581 -15.0407 207 215 (R)(L)
1418.7427 1418.7929 -35.3574 316 328 (K)EITALAPSTMKIK(I) 1Met-ox
1515.7867 1515.7497 24.3953 85 95 (K)(V)
1744.8629 1744.8870 -13.7884 192 206 (K)(E)
1790.9126 1790.8925 11.2679 239 254 (K)(F)
1954.0752 1954.0650 5.2329 96 113 (R)(A)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1014.4946	1014.4818	12.6163	184	191	(R)DLTDYLMK(I)	1Met-ox
1129.6411	1129.6581	-15.0407	207	215	(R)(L)
1418.7427	1418.7929	-35.3574	316	328	(K)EITALAPSTMKIK(I)	1Met-ox
1515.7867	1515.7497	24.3953	85	95	(K)(V)
1744.8629	1744.8870	-13.7884	192	206	(K)(E)
1790.9126	1790.8925	11.2679	239	254	(K)(F)
1954.0752	1954.0650	5.2329	96	113	(R)(A)

The matched peptides cover 24% (90/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.2 Mean Error = -1.5249 ppm Data Tolerance = 41.3026 ppm Mean Missed Cleavages = 0.6

8. 7/46 matches (15%). 41793.2 Da, pI = 5.31. Acc. # P02571. HUMAN. ACTIN, CYTOPLASMIC 2 (GAMMA-ACTIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1014.4946 1014.4818 12.6163 184 191 (R)DLTDYLMK(I) 1Met-ox
1129.6411 1129.6581 -15.0407 207 215 (R)(L)
1418.7427 1418.7929 -35.3574 316 328 (K)EITALAPSTMKIK(I) 1Met-ox
1515.7867 1515.7497 24.3953 85 95 (K)(V)
1744.8629 1744.8870 -13.7884 192 206 (K)(E)
1790.9126 1790.8925 11.2679 239 254 (K)(F)
1954.0752 1954.0650 5.2329 96 113 (R)(A)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1014.4946	1014.4818	12.6163	184	191	(R)DLTDYLMK(I)	1Met-ox
1129.6411	1129.6581	-15.0407	207	215	(R)(L)
1418.7427	1418.7929	-35.3574	316	328	(K)EITALAPSTMKIK(I)	1Met-ox
1515.7867	1515.7497	24.3953	85	95	(K)(V)
1744.8629	1744.8870	-13.7884	192	206	(K)(E)
1790.9126	1790.8925	11.2679	239	254	(K)(F)
1954.0752	1954.0650	5.2329	96	113	(R)(A)

The matched peptides cover 24% (90/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.2 Mean Error = -1.5249 ppm Data Tolerance = 41.3026 ppm Mean Missed Cleavages = 0.6

9. 6/46 matches (13%). 70424.7 Da, pI = 8.51. Acc. # Q01405. MOUSE. PROTEIN TRANSPORT PROTEIN SEC23A (SEC23-RELATED PROTEIN A).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1385.7553 1385.8231 -48.9014 34 46 (R)(E)
1442.8237 1442.8041 13.5762 408 421 (K)(G)
1744.8629 1744.8870 -13.7884 90 105 (R)(E)
1812.9699 1813.0257 -30.8067 405 421 (R)(G)
1994.0540 1993.9952 29.4930 64 79 (R)(A)
1999.0761 1999.1010 -12.4808 193 210 (K)(G)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1385.7553	1385.8231	-48.9014	34	46	(R)(E)
1442.8237	1442.8041	13.5762	408	421	(K)(G)
1744.8629	1744.8870	-13.7884	90	105	(R)(E)
1812.9699	1813.0257	-30.8067	405	421	(R)(G)
1994.0540	1993.9952	29.4930	64	79	(R)(A)
1999.0761	1999.1010	-12.4808	193	210	(K)(G)

The matched peptides cover 12% (80/623AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.0 Mean Error = -10.4847 ppm Data Tolerance = 57.1375 ppm Mean Missed Cleavages = 0.5

10. 4/46 matches (8%). 30658.9 Da, pI = 8.84. Acc. # Q9Y277. HUMAN. VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL PROTEIN 3 (VDAC-3) (HVDAC3) (OUTER MITOCHONDRIAL MEMBRANE PROTEIN PORIN 3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1344.6995 1344.6622 27.7267 64 74 (K)(W)
1418.7427 1418.7895 -32.9816 97 109 (K)(K)
1731.9476 1731.8892 33.7199 13 28 (K)(I)
1847.8582 1847.9252 -36.2112 202 218 (K)(F)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1344.6995	1344.6622	27.7267	64	74	(K)(W)
1418.7427	1418.7895	-32.9816	97	109	(K)(K)
1731.9476	1731.8892	33.7199	13	28	(K)(I)
1847.8582	1847.9252	-36.2112	202	218	(K)(F)

The matched peptides cover 20% (57/283AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 8.7 Mean Error = -1.9365 ppm Data Tolerance = 75.6293 ppm Mean Missed Cleavages = 0.5