MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D20
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1163 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/83(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.830e+005	8 (9)	24.0	9.6	1.68	47.0		49896/5.0		HUMAN	Tubulin alpha-6 chain (Alpha-tubulin 6)
2	2.248e+005	11 (13)	20.0	13.3	3.39	50.5		68042/5.8		HUMAN	Protein Tara (Trio-associated repeat on actin)
3	9.937e+004	12 (14)	22.0	14.5	19.1	41.2		45375/4.4		HUMAN	Nucleosome assembly protein 1-like 1 (NAP-1 related protein)
4	4.788e+004	7 (8)	14.0	8.4	16.3	47.3		75493/7.6		HUMAN	Thymopoietin, isoform alpha (TP alpha)
5	4.183e+004	7 (8)	20.0	8.4	-4.72	32.3		49910/5.0		MOUSE	Tubulin alpha-6 chain (Alpha-tubulin 6)

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Detailed Results

1. 8/83 matches (9%).
Acc. #: Species: HUMAN Name: Tubulin alpha-6 chain (Alpha-tubulin 6)
Index: MW: 49896 Da pI: 5.0

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1085.6129	1085.6206	-7.1		113	121	0	(K) (I)
1487.8330	1487.8797	-31		230	243	0	(R) (F)
1701.9356	1701.9063	17		65	79	0	(R) (T)
1718.8963	1718.8826	8.0		216	229	0	(R) (L)
1756.9414	1756.9638	-13		265	280	0	(R) (A)
1841.9906	1842.0125	-12		106	121	1	(R) (I)
1864.9917	1864.9050	46		374	390	0	(R) (L)
1989.0656	1989.0558	4.9		80	96	1	(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (109/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/83 matches (13%).
Acc. #: Species: HUMAN Name: Protein Tara (Trio-associated repeat on actin)
Index: MW: 68042 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
975.5118 975.5363 -25 250 257 1 (R) (W)
1022.4626 1022.4651 -2.4 533 540 0 (R) (D)
1154.5339 1154.5806 -40 418 427 0 (K) (R)
1169.6202 1169.6279 -6.6 312 322 0 (R) (S)
1219.6935 1219.6356 47 506 515 0 (R) (V)
1332.7421 1332.6952 35 38 48 0 (K) (Y)
1392.6756 1392.6872 -8.3 286 298 1 (R) (E)
1442.6705 1442.6586 8.2 69 80 0 (R) (N)
1814.9216 1814.9230 -0.76 38 51 1 (K) (D)
2227.1703 2227.1206 22 237 256 1 (R) (K)
2243.1954 2243.1784 7.6 169 188 1 (R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (119/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/83 matches (14%).
Acc. #: Species: HUMAN Name: Nucleosome assembly protein 1-like 1 (NAP-1 related protein)
Index: MW: 45375 Da pI: 4.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1336.6714 1336.6174 40 95 104 0 (K) (K)
1336.6714 1336.7305 -44 166 176 0 (K) (N)
1356.7523 1356.7204 24 106 116 0 (K) (R)
1464.7530 1464.7123 28 95 105 1 (K) (Y)
1484.8417 1484.8153 18 105 116 1 (R) (R)
1512.8651 1512.8215 29 106 117 1 (K) (F)
1860.0272 1859.9755 28 56 72 0 (R) (V)
2095.0550 2095.0381 8.0 177 194 0 (K) (H)
2111.1034 2111.0331 33 1Met-ox 177 194 0 (K)NVDLLSDMVQEHDEPILK(H)
2211.1685 2211.1266 19 pyroGlu 1Met-ox 37 55 0 (R)QLTVQMMQNPQILAALQER(L)
2227.1703 2227.1215 22 pyroGlu 2Met-ox 37 55 0 (R)QLTVQMMQNPQILAALQER(L)
2228.1951 2228.1531 19 1Met-ox 37 55 0 (R)QLTVQMMQNPQILAALQER(L)
2244.2042 2244.1480 25 2Met-ox 37 55 0 (R)QLTVQMMQNPQILAALQER(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (88/391AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/83 matches (8%).
Acc. #: Species: HUMAN Name: Thymopoietin, isoform alpha (TP alpha)
Index: MW: 75493 Da pI: 7.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1085.6129 1085.6206 -7.1 526 535 0 (R) (V)
1350.6852 1350.6694 12 417 427 0 (K) (K)
1353.7318 1353.7127 14 72 86 0 (R) (S)
1505.8229 1505.7562 44 1 13 0 (-) (D)
1647.8502 1647.8190 19 158 173 0 (R) (Q)
1718.8963 1718.9230 -16 35 48 0 (K) (N)
1989.0656 1988.9711 47 592 610 0 (K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (98/694AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/83 matches (8%).
Acc. #: Species: MOUSE Name: Tubulin alpha-6 chain (Alpha-tubulin 6)
Index: MW: 49910 Da pI: 5.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1085.6129 1085.6206 -7.1 113 121 0 (K) (I)
1487.8330 1487.8797 -31 230 243 0 (R) (F)
1701.9356 1701.9063 17 65 79 0 (R) (T)
1718.8963 1718.8826 8.0 216 229 0 (R) (L)
1756.9414 1756.9638 -13 265 280 0 (R) (A)
1841.9906 1842.0125 -12 106 121 1 (R) (I)
1989.0656 1989.0558 4.9 80 96 1 (R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (92/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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