MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D21
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 305 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/45(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.732e+007	11 (24)	27.0	24.4	13.1	16.1		42824/4.6		HUMAN	Nucleosome assembly protein 1-like 4 (Nucleosome assembly protein 2) (NAP2)
2	1.480e+006	11 (24)	26.0	24.4	3.63	42.3		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
3	7123	8 (17)	13.0	17.8	6.10	26.2		59519/5.1		HUMAN	Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
4	1461	8 (17)	13.0	17.8	-15.0	61.2		53675/5.5		HUMAN	Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
5	1311	6 (13)	8.0	13.3	7.55	56.0		82806/9.1		HUMAN	Poly(A) polymerase gamma (PAP gamma) (Polynucleotide adenylyltransferase gamma) (SRP RNA 3' adenylating enzyme)

40853	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	x	x	.	.	.	.	x	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.	x	x	.	.	.	x
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28336	.	.	.	x	x	.	.	.	.	x	.	.	.	x	.	.	x	x	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
112017	.	.	x	.	x	.	.	.	x	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.

Detailed Results

1. 11/45 matches (24%).
Acc. #: Species: HUMAN Name: Nucleosome assembly protein 1-like 4 (Nucleosome assembly protein 2) (NAP2)
Index: MW: 42824 Da pI: 4.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1201.6077	1201.5999	6.4		27	36	0	(K) (V)
1217.5969	1217.5948	1.7	1Met-ox	27	36	0	(K)LTDQVMQNPR(V)
1328.7042	1328.6891	11		95	105	0	(K) (R)
1336.6287	1336.6174	8.5		84	93	0	(K) (K)
1412.7537	1412.7367	12		158	168	0	(K) (N)
1456.8050	1456.7840	14		94	105	1	(R) (R)
1464.7331	1464.7123	14		84	94	1	(K) (Y)
1484.8039	1484.7902	9.2		95	106	1	(K) (R)
1827.9399	1827.9070	18		283	297	0	(K) (A)
1911.0148	1910.9864	15		45	61	0	(R) (A)
2978.3954	2978.2968	33		298	324	0	(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (103/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/45 matches (24%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1033.5198 1033.5166 3.1 484 492 0 (R) (M)
1179.5972 1179.6010 -3.2 377 386 0 (K) (H)
1217.5969 1217.6166 -16 634 644 0 (R) (-)
1277.6965 1277.6337 49 247 257 1 (K) (N)
1277.6965 1277.7105 -11 473 483 0 (K) (T)
1302.6770 1302.7018 -19 393 403 1 (R) (V)
1302.6770 1302.7157 -30 344 355 0 (R) (A)
1357.7155 1357.6963 14 444 455 0 (K) (E)
1383.7032 1383.6909 8.9 186 197 0 (K) (V)
1475.7756 1475.7494 18 212 223 0 (K) (T)
1475.7756 1475.7858 -6.9 200 211 0 (R) (W)
1638.8927 1638.8604 20 186 199 1 (K) (F)
1687.9610 1687.9707 -5.7 66 82 1 (R) (G)
2384.0236 2383.9524 30 519 549 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (173/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/45 matches (17%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1165.6056 1165.5853 17 442 450 0 (R) (S)
1201.6077 1201.6177 -8.3 246 256 0 (R) (R)
1234.6640 1234.6796 -13 236 245 1 (R) (Q)
1357.7155 1357.7188 -2.4 246 257 1 (R) (V)
1434.7734 1434.7705 2.0 440 450 1 (K) (S)
1493.7593 1493.7348 16 323 334 1 (R) (D)
1707.8103 1707.7727 22 41 59 0 (K) (G)
2097.0112 2096.9810 14 423 439 0 (R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (81/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/45 matches (17%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
Index: MW: 53675 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1033.5198 1033.5213 -1.4 1Met-ox 365 372 1 (K)QDMARQLR(E)
1045.5466 1045.5530 -6.1 150 158 0 (R) (L)
1201.6077 1201.6541 -39 149 158 1 (R) (L)
1277.6965 1277.7105 -11 382 392 0 (K) (K)
1320.6119 1320.6721 -46 253 264 0 (R) (A)
1328.7042 1328.7538 -37 353 364 1 (K) (Q)
1336.6287 1336.6670 -29 1Met-ox 253 264 0 (R)SLDMDSIIAEVK(A)
1412.7537 1412.6844 49 274 285 1 (R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (65/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/45 matches (13%).
Acc. #: Species: HUMAN Name: Poly(A) polymerase gamma (PAP gamma) (Polynucleotide adenylyltransferase gamma) (SRP RNA 3' adenylating enzyme)
Index: MW: 82806 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
982.5083 982.4919 17 334 341 0 (R) (Q)
1045.5466 1045.5464 0.15 469 477 0 (R) (E)
1179.5972 1179.5534 37 73 82 0 (K) (N)
1232.6251 1232.6679 -35 360 369 0 (K) (Y)
1350.6811 1350.6324 36 3 14 0 (K) (Q)
1911.0148 1911.0340 -10 205 220 1 (R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (65/736AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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