MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D25
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 438 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/50(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1408	10 (20)	19.0	20.0	-86.6	23.9		45375/4.4		HUMAN	Nucleosome assembly protein 1-like 1 (NAP-1 related protein)
2	1273	5 (10)	7.0	10.0	-49.9	80.5		87449/9.1		HUMAN	Zinc finger protein 267 (Zinc finger protein HZF2)
3	991	5 (10)	20.0	10.0	-38.1	74.6		42995/9.5		HUMAN	SNRNA ACTIVATING PROTEIN COMPLEX 43 KDA SUBUNIT (SNAPC 43 KDA SUBUNIT) (PROXIMAL SEQUENCE ELEMENT-BINDING TRANSCRIPTION FACTOR GAMMA SUBUNIT) (PSE-BINDING FACTOR GAMMA SUBUNIT) (PTF GAMMA SUBUNIT)
4	633	7 (14)	11.0	14.0	-64.3	74.5		59454/9.2		MOUSE	Cytochrome P450-CC24, mitochondrial precursor (P450-CC24) (Vitamin D(3) 24-hydroxylase) (1,25-dihydroxyvitamin D(3) 24-hydroxylase) (24-OHASE)
5	631	5 (10)	11.0	10.0	-90.2	41.2		60286/6.2		MOUSE	ALKALINE PHOSPHATASE, INTESTINAL PRECURSOR (IAP)

35690	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	x	.	x	x	.	.	.	.	x	.	.	x	.	x	x	x
64775	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x
5664	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	x	.	x	.
37148	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	x	.	.	x	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.
61817	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	x	x

Detailed Results

1. 10/50 matches (20%).
Acc. #: Species: HUMAN Name: Nucleosome assembly protein 1-like 1 (NAP-1 related protein)
Index: MW: 45375 Da pI: 4.4

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1336.5216	1336.6174	-72		95	104	0	(K) (K)
1356.5789	1356.7204	-104		106	116	0	(K) (R)
1464.5934	1464.7123	-81		95	105	1	(K) (Y)
1484.6520	1484.8153	-110		105	116	1	(R) (R)
1512.6970	1512.8215	-82		106	117	1	(K) (F)
1859.8226	1859.9755	-82		56	72	0	(R) (V)
2110.8713	2111.0331	-77	1Met-ox	177	194	0	(K)NVDLLSDMVQEHDEPILK(H)
2210.9346	2211.1266	-87	pyroGlu 1Met-ox	37	55	0	(R)QLTVQMMQNPQILAALQER(L)
2227.9545	2228.1531	-89	1Met-ox	37	55	0	(R)QLTVQMMQNPQILAALQER(L)
2243.9648	2244.1480	-82	2Met-ox	37	55	0	(R)QLTVQMMQNPQILAALQER(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (77/391AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/50 matches (10%).
Acc. #: Species: HUMAN Name: Zinc finger protein 267 (Zinc finger protein HZF2)
Index: MW: 87449 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1060.4283 1060.5176 -84 709 717 0 (R) (C)
1179.4982 1179.4853 11 215 223 0 (K) (T)
1251.5721 1251.6077 -28 272 281 0 (K) (H)
1464.5934 1464.7018 -74 103 114 0 (R) (K)
2243.9648 2244.1301 -74 168 186 0 (K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (59/743AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/50 matches (10%).
Acc. #: Species: HUMAN Name: SNRNA ACTIVATING PROTEIN COMPLEX 43 KDA SUBUNIT (SNAPC 43 KDA SUBUNIT) (PROXIMAL SEQUENCE ELEMENT-BINDING TRANSCRIPTION FACTOR GAMMA SUBUNIT) (PSE-BINDING FACTOR GAMMA SUBUNIT) (PTF GAMMA SUBUNIT)
Index: MW: 42995 Da pI: 9.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1151.6088 1151.6285 -17 324 333 1 (R) (E)
1631.7113 1631.7528 -25 1Met-ox 26 37 1 (R)FEDFTELWRNMK(F)
1859.8226 1859.8995 -41 1 17 0 (-) (F)
2183.0544 2183.0674 -6.0 110 127 0 (K) (K)
2227.9545 2228.1789 -101 76 94 0 (R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (76/368AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/50 matches (14%).
Acc. #: Species: MOUSE Name: Cytochrome P450-CC24, mitochondrial precursor (P450-CC24) (Vitamin D(3) 24-hydroxylase) (1,25-dihydroxyvitamin D(3) 24-hydroxylase) (24-OHASE)
Index: MW: 59454 Da pI: 9.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
864.3379 864.3959 -67 1 7 0 (-) (R)
1051.6004 1051.6013 -0.84 18 26 1 (R) (S)
1125.4558 1125.5839 -114 281 289 0 (K) (L)
1251.5721 1251.6594 -70 373 382 1 (R) (E)
1356.5789 1356.7132 -99 1Met-ox 252 262 1 (K)MMVTPVELHKR(L)
1631.7113 1631.7773 -40 40 53 0 (K) (N)
1647.6752 1647.7722 -59 1Met-ox 40 53 0 (K)EVPLCPLMTDGETR(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (60/514AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/50 matches (10%).
Acc. #: Species: MOUSE Name: ALKALINE PHOSPHATASE, INTESTINAL PRECURSOR (IAP)
Index: MW: 60286 Da pI: 6.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
868.4579 868.5369 -91 439 446 1 (R) (S)
1566.6036 1566.7011 -62 186 199 0 (R) (E)
2110.8713 2111.0371 -79 276 294 0 (K) (F)
2227.9545 2228.2073 -113 204 224 1 (K) (Y)
2243.9648 2244.2022 -106 1Met-ox 204 224 1 (K)DIATQLISNMDINVILGGGRK(Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (62/559AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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