MS-Fit Search Results

MS-Fit Search Results

.

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D26
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 381 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/44(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	8.966e+005	11 (25)	19.0	25.0	-69.0	72.4		61988/5.1		HUMAN	Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
2	1.895e+005	9 (20)	35.0	20.5	-67.2	46.3		37107/4.5		HUMAN	Calumenin precursor (IEF SSP 9302)
3	7219	8 (18)	15.0	18.2	-56.8	86.7		84922/5.4		MOUSE	Calpain inhibitor (Calpastatin)
4	7196	8 (18)	21.0	18.2	-59.8	49.8		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
5	3545	9 (20)	18.0	20.5	-64.3	86.0		67255/9.1		MOUSE	Zinc finger protein 37 (Zfp-37) (Male germ cell specific zinc finger protein)

100557	.	.	.	.	x	x	.	x	.	.	x	.	x	x	.	.	.	.	.	.	.	.	.	.	x	.	x	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.
43778	x	.	.	x	.	.	.	.	.	x	x	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	.
80785	x	.	.	.	x	.	.	.	.	.	x	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.
46726	.	.	.	.	.	.	.	x	x	.	x	.	.	.	x	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.
94952	.	.	.	.	.	x	.	x	x	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	x	.	x	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.

Detailed Results

1. 11/44 matches (25%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1121.4490	1121.5843	-121		327	335	0	(R) (N)
1140.4629	1140.5649	-89	pyroGlu	250	260	0	(R)QGVDADINGLR(Q)
1157.4905	1157.5915	-87		250	260	0	(R) (Q)
1277.6192	1277.5875	25		29	40	1	(R) (G)
1307.5922	1307.6782	-66		240	249	1	(R) (Q)
1323.5623	1323.6731	-84	1Met-ox	240	249	1	(R)IKFEMEQNLR(Q)
1791.6272	1791.7283	-56		490	512	0	(R) (G)
1837.8108	1837.9660	-84		374	389	0	(R) (K)
1851.8110	1851.9275	-63		321	335	1	(K) (N)
1867.8163	1867.9224	-57	1Met-ox	321	335	1	(K)TLNDMRQEYEQLIAK(N)
2704.9548	2705.1617	-76		63	94	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (119/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/44 matches (20%).
Acc. #: Species: HUMAN Name: Calumenin precursor (IEF SSP 9302)
Index: MW: 37107 Da pI: 4.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
954.3940 954.4685 -78 235 241 0 (R) (D)
1109.4405 1109.5267 -78 104 111 0 (R) (Q)
1232.5099 1232.5911 -66 272 281 0 (R) (D)
1277.6192 1277.6411 -17 162 173 1 (K) (E)
1340.5657 1340.6599 -70 232 241 1 (K) (D)
1579.6576 1579.7604 -65 60 72 1 (K) (L)
1887.7463 1887.8513 -56 256 271 0 (K) (H)
2478.9043 2479.0802 -71 38 59 0 (K) (T)
2501.0360 2501.2970 -104 5 25 1 (R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (112/315AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/44 matches (18%).
Acc. #: Species: MOUSE Name: Calpain inhibitor (Calpastatin)
Index: MW: 84922 Da pI: 5.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
954.3940 954.4169 -24 523 530 0 (R) (T)
1121.4490 1121.5340 -76 22 30 0 (R) (N)
1277.6192 1277.6351 -12 147 158 1 (K) (E)
1323.5623 1323.7133 -114 97 107 1 (K) (E)
1579.6576 1579.7274 -44 165 178 0 (R) (T)
1579.6576 1579.7968 -88 459 472 1 (R) (S)
1867.8163 1867.8925 -41 442 458 0 (K) (S)
1939.8400 1939.8344 2.9 405 420 1 (R) (L)
2282.9938 2283.2560 -115 500 522 1 (K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (124/788AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/44 matches (18%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1157.4905 1157.5149 -21 1Met-ox 464 472 0 (R)DYQELMNTK(L)
1179.5082 1179.6010 -79 377 386 0 (K) (H)
1277.6192 1277.6337 -11 247 257 1 (K) (N)
1277.6192 1277.7105 -71 473 483 0 (K) (T)
1340.5657 1340.6698 -78 365 376 1 (K) (Y)
1475.6564 1475.7494 -63 212 223 0 (K) (T)
1475.6564 1475.7858 -88 200 211 0 (R) (W)
1638.7661 1638.8604 -58 186 199 1 (K) (F)
1993.8360 1993.9772 -71 224 239 0 (R) (R)
2383.8128 2383.9524 -59 519 549 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (138/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/44 matches (20%).
Acc. #: Species: MOUSE Name: Zinc finger protein 37 (Zfp-37) (Male germ cell specific zinc finger protein)
Index: MW: 67255 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1140.4629 1140.5836 -106 pyroGlu 574 583 1 (K)QNASLTKHMK(I)
1157.4905 1157.6101 -103 574 583 1 (K) (I)
1179.5082 1179.5945 -73 406 415 1 (K) (I)
1340.5657 1340.6745 -81 237 247 1 (K) (A)
1493.6468 1493.6960 -33 459 471 0 (K) (T)
1791.6272 1791.7642 -76 1Met-ox 33 48 0 (K)LENCSNPASMGNQDPK(Q)
1837.8108 1837.8213 -5.7 360 374 1 (R) (S)
1851.8110 1851.8006 5.6 304 318 0 (R) (A)
2383.8128 2384.0651 -106 276 295 0 (R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (110/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.