MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D29
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1991 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/91(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.218e+00610 (10)24.011.0-41.079.0 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
22.892e+00514 (15)26.015.4-50.598.3 96821/5.5HUMANTuftelin-interacting protein 11 (HSPC006)
31.593e+00511 (12)21.012.1-71.294.9 76725/6.9MOUSESerotransferrin precursor (Transferrin) (Siderophilin) (Beta-1-metal binding globulin)
49.166e+0048 (8)14.08.8-59.596.2 86702/5.8HUMANRal guanine nucleotide dissociation stimulator-like 1 (RalGDS-like 1)
58.825e+0048 (8)20.08.8-78.164.1 59556/5.4MOUSET-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
66.469e+0047 (7)28.07.7-55.369.6 35620/9.2HUMANSideroflexin 1
75.168e+00410 (10)20.011.0-56.381.3 65866/8.1HUMANKeratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
84.999e+0049 (9)18.09.9-69.070.4 89424/9.5HUMANMyosin If (Myosin-IE)
94.795e+0048 (8)15.08.8-53.3130 82343/7.1MOUSEOrigin recognition complex subunit 3 (Origin recognition complex subunit Latheo)
103.338e+00410 (10)17.011.0-67.978.7 70582/8.7HUMAN5-aminolevulinic acid synthase, nonspecific, mitochondrial precursor (Delta-aminolevulinate synthase) (Delta-ALA synthetase) (ALAS-H)
45313....x.............x....x...x....xx.......................x.....x....x....x.................
27058x.................xx.....x.x.xx..xx...........x........x.........x.x......x................
27784.......x..x.........x..........x.....x..........x.......x......x...x..........xx...........
87265x..............x...x............x..................................x............x.x.....x..
84429.............x........x......x.x...............x.....x...................x..x..............
3426.................x..............................x....x...........x.......xx.x..............
110808....x......x....x.......x.x......x.............x.....x........x..........x.................
109059x.............................x................x.....x................x...x.x.....xx.......
8526.....................x...xx.............xx......................x..................x..x....
85421......x.........x......x.xx....xx...........................x..........x.x.................


Detailed Results

1. 10/91 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.4457973.5318-883964030(K) (V)
1141.48721141.5199-294644720(R) (L)
1179.52061179.6010-683773860(K) (H)
1277.63531277.63371.22472571(K) (N)
1277.63531277.7105-594734830(K) (T)
1383.59551383.6909-691861970(K) (V)
1475.67261475.7494-522122230(K) (T)
1475.67261475.7858-772002110(R) (W)
1993.87731993.9772-502242390(R) (R)
2149.97302150.0783-492242401(R) (R)
2308.10302307.99144839651(R) (S)
2383.95092383.9524-0.645195490(R) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 24% (160/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/91 matches (15%).
Acc. #: Species: HUMAN Name: Tuftelin-interacting protein 11 (HSPC006)
Index: MW: 96821 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
964.3463964.4158-721Met-ox 7537600(R)EAENMAQR(G)
1141.48721141.6118-1094914990(R) (N)
1151.47711151.5809-901861971(K) (T)
1258.48371258.5738-723593680(K) (V)
1277.63531277.5261851Met-ox 1381470(K)SFMDFGSWER(H)
1277.63531277.7040-541Met-ox 1561661(K)LLQKMGYVPGR(G)
1307.60491307.59755.63763850(K) (R)
1346.64341346.6705-207297390(R) (R)
1475.67261475.7873-784804900(R) (N)
1475.67261475.7892-792622741(K) (E)
1481.64801481.7059-391Met-ox 7027131(K)DKFNEALDIMNR(A)
1481.64801481.7640-7873860(R) (K)
1834.90191835.0431-778078221(R) (T)
1934.88701934.8410243874020(R) (I)
2157.90032158.0893-882893080(K) (E)
2289.98702290.1178-57pyroGlu 1Met-ox 3413581(R)QLQYERDMVVNLFHELEK(M)
2395.04712395.1909-601Met-ox 6827011(K)WYLGWKSMFSDQVLAHPSVK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (221/837AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/91 matches (12%).
Acc. #: Species: MOUSE Name: Serotransferrin precursor (Transferrin) (Siderophilin) (Beta-1-metal binding globulin)
Index: MW: 76725 Da pI: 6.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1026.37981026.4930-1106516581(R) (L)
1049.40451049.5202-1101Met-ox 3243311(R)VPPRMDYR(L)
1158.50761158.6411-1152792870(K) (V)
1349.52271349.6701-1094564670(K) (G)
1349.52271349.6701-1091231340(K) (K)
1534.62961534.7865-1024564691(K) (K)
1841.85171841.7785403843980(K) (I)
1949.86171949.9067-235325471(K) (G)
2149.97302150.0929-565936111(K) (E)
2289.98702290.1047-514794971(R) (F)
2550.05632550.1804-494714921(K) (I)
2571.07032571.2223-596596811(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (151/697AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/91 matches (8%).
Acc. #: Species: HUMAN Name: Ral guanine nucleotide dissociation stimulator-like 1 (RalGDS-like 1)
Index: MW: 86702 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
964.3463964.4410-987347410(K) (L)
1099.50381099.6363-1211081160(K) (Y)
1151.47711151.5982-1051Met-ox 3763851(R)ELLMKEGTSK(F)
1383.59551383.6215-196546650(R) (S)
2289.98702289.9417201171370(R) (M)
2663.17702663.2775-389311(K) (V)
2679.15922679.2725-421Met-ox 9311(K)MSSIQDWGEEVEEGAVYHVTLKR(V)
2902.23172902.4449-737077310(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (108/768AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/91 matches (8%).
Acc. #: Species: MOUSE Name: T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
Index: MW: 59556 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.45301065.5217-643914000(R) (V)
1169.47051169.5512-692612700(K) (G)
1307.60491307.6384-2621310(K) (N)
1349.52271349.6887-1231Met-ox 8201(K)APGFAQMLKDGAK(H)
1838.84151838.9574-631211370(R) (K)
1890.86731891.0475-955215390(R) (K)
2383.95092384.1114-672612811(K) (A)
2463.94112464.2295-1171822030(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (113/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 7/91 matches (7%).
Acc. #: Species: HUMAN Name: Sideroflexin 1
Index: MW: 35620 Da pI: 9.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1109.45651109.5380-7316240(R) (A)
1841.85171841.8710-1171861(R) (M)
1890.86731891.0078-74pyroGlu 56721(R)QGIVPPGLTENELWRAK(Y)
2157.90032158.1478-1152342530(R) (K)
2383.95092384.0404-38931120(R) (T)
2395.04712395.1584-4616351(R) (N)
2463.94112464.0149-305Met-ox 931120(R)MSAQVPMNMTITGCMMTFYR(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (91/322AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 10/91 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.4457973.5318-884004070(K) (V)
1057.43201057.4480-151Met-ox 180(-)MSCQISCK(S)
1107.49741107.5435-423603680(K) (S)
1254.46911254.6079-11121340(R) (R)
1263.61341263.6949-644774870(K) (K)
1475.67261475.7858-772042150(R) (W)
1838.84151838.9149-4071920(K) (G)
1890.86731890.85824.85796011(R) (H)
2143.90532143.9454-191Met-ox 2542710(R)TSQNSELNNMQDLVEDYK(K)
2383.95092384.2172-1124684871(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (134/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 9/91 matches (9%).
Acc. #: Species: HUMAN Name: Myosin If (Myosin-IE)
Index: MW: 89424 Da pI: 9.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
964.3463964.4423-1009161(K) (S)
1346.64341346.7109-503173270(R) (W)
1838.84151838.8958-303964101(K) (K)
1890.86731891.0230-823133271(K) (W)
2342.88262343.1542-1161Met-ox 2052240(R)DVLFSDLIELMQSSDQAFLR(M)
2395.04712395.2120-691161341(R) (L)
2463.94112464.1560-875115310(R) (T)
2679.15922679.3809-836566781(K) (R)
2682.24002682.2517-4.46827050(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (145/782AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/91 matches (8%).
Acc. #: Species: MOUSE Name: Origin recognition complex subunit 3 (Origin recognition complex subunit Latheo)
Index: MW: 82343 Da pI: 7.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1165.50781165.6403-1141Met-ox 5515591(K)KPTKFEMLR(E)
1258.48371258.6294-11616250(K) (K)
1263.61341263.5163775165260(K) (G)
1559.64431559.6656-141581690(K) (H)
1565.60471565.7746-1093613730(R) (R)
2150.99712151.0619-307251(R) (K)
2682.24002682.4156-655966181(R) (N)
2737.20472737.3581-563363580(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (110/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 10/91 matches (10%).
Acc. #: Species: HUMAN Name: 5-aminolevulinic acid synthase, nonspecific, mitochondrial precursor (Delta-aminolevulinate synthase) (Delta-ALA synthetase) (ALAS-H)
Index: MW: 70582 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1023.39421023.4933-971691760(K) (Q)
1107.49741107.5542-512622710(R) (Q)
1179.52061179.5911-602122201(K) (T)
1258.48371258.6214-1094234351(R) (M)
1263.61341263.7061-731821920(R) (S)
1349.52271349.6458-911081180(K) (G)
1383.59551383.58845.11Met-ox 5405500(K)NTEVCDELMSR(H)
2063.96432064.0072-211581761(K) (Q)
2347.06342347.2046-601822011(R) (F)
2383.95092384.2405-1215135330(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (114/640AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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