MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D30
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 136 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/75(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.499e+0068 (10)35.010.73.8799.7 38890/4.9HUMANReticulocalbin 1 precursor
25.841e+0045 (6)21.06.723.378.3 38113/4.7MOUSEReticulocalbin 1 precursor
376327 (9)9.09.3-3.6188.2 81610/9.0HUMANZinc finger protein 175 (Zinc finger protein OTK18)
444746 (8)12.08.0-21.685.3 82673/6.3HUMANDiacylglycerol kinase, alpha (Diglyceride kinase) (DGK-alpha) (DAG kinase alpha) (80 kDa diacylglycerol kinase)
524894 (5)13.05.3-6.46126 67625/5.5MOUSEProtein kinase C, zeta type (nPKC-zeta)
617006 (8)10.08.0-41.682.3 53498/5.2MOUSEDesmin
716996 (8)10.08.0-41.682.3 53536/5.2HUMANDesmin
88805 (6)14.06.7-53.857.2 47586/5.9MOUSETranscobalamin II precursor (TCII) (TC II)
98795 (6)11.06.7-34.583.6 80642/6.6HUMANProtein-tyrosine phosphatase epsilon precursor (R-PTP-epsilon)
108365 (6)9.06.7-35.6114 50382/4.7HUMANNucleolar autoantigen No55
60425...........x...........................................xx...x..xxx.......x.
20661.......................................................x....x..xx........x.
15188.........x..x..........................................xx......xx........x.
79236.............................x..........................xx..xx.......x.....
43518..................................................x................x....xx.
74992...........x.....x...x..x............................................xx....
89993...........x.....x...x..x............................................xx....
19934....................................................x..........xx...x..x...
84524...........................................x.......x........x......x..x....
4261.........x..x................................x......x..x...................


Detailed Results

1. 8/75 matches (10%).
Acc. #: Species: HUMAN Name: Reticulocalbin 1 precursor
Index: MW: 38890 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
969.4149969.4430-292502560(R) (D)
969.4149969.5046-931151220(R) (V)
1515.75481515.8158-402052170(K) (N)
1565.74171565.7447-1.971831(K) (L)
1949.95661949.9258162712860(R) (H)
2020.99062020.9115391892040(R) (E)
2036.98602036.9064391Met-ox 1892040(R)EEFTAFLHPEEFEHMK(E)
2136.13572136.046142871051(K) (T)
2785.40252785.2283631421650(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 35% (116/331AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/75 matches (6%).
Acc. #: Species: MOUSE Name: Reticulocalbin 1 precursor
Index: MW: 38113 Da pI: 4.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1515.75481515.8158-401992110(K) (N)
1949.95661949.9258162652800(R) (H)
2020.99062020.9115391831980(R) (E)
2036.98602036.9064391Met-ox 1831980(R)EEFTAFLHPEEFEHMK(E)
2785.40252785.2283631361590(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 21% (69/325AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/75 matches (9%).
Acc. #: Species: HUMAN Name: Zinc finger protein 175 (Zinc finger protein OTK18)
Index: MW: 81610 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
954.3538954.4355-861201270(K) (D)
970.4003970.4304-311Met-ox 1201270(K)DMVGEFTR(D)
1515.75481515.7266191151271(K) (D)
1565.74171565.7672-1684961(K) (C)
2020.99062020.9187361481640(K) (K)
2036.98602036.9136361Met-ox 1481640(K)DEQNQIQPMSHSAFFNK(K)
2785.40252785.3527184604821(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (66/711AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/75 matches (8%).
Acc. #: Species: HUMAN Name: Diacylglycerol kinase, alpha (Diglyceride kinase) (DGK-alpha) (DAG kinase alpha) (80 kDa diacylglycerol kinase)
Index: MW: 82673 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1039.40321039.4770-7133410(K) (Y)
1565.74171565.7481-4.13543670(K) (I)
1698.88881698.9543-394054191(R) (L)
1949.95661950.0258-356546710(R) (L)
1963.06611962.96025442580(K) (I)
2369.04322369.1245-347157351(K) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (94/735AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/75 matches (5%).
Acc. #: Species: MOUSE Name: Protein kinase C, zeta type (nPKC-zeta)
Index: MW: 67625 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1215.47261215.4887-131411500(R) (I)
2282.28482282.1466613263440(R) (Q)
2781.00272781.2515-894254480(R) (S)
2785.40252785.3572165695921(K) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (77/592AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 6/75 matches (8%).
Acc. #: Species: MOUSE Name: Desmin
Index: MW: 53498 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
969.4149969.381035pyroGlu 3183240(K)QEMMEYR(H)
986.3366986.4076-723183240(K) (H)
1001.29721001.3708-74pyroGlu 2Met-ox 3183240(K)QEMMEYR(H)
1018.36911018.3974-282Met-ox 3183240(K)QEMMEYR(H)
2369.04322369.1585-494494691(K) (-)
2384.06802384.2172-633864051(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (48/469AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 6/75 matches (8%).
Acc. #: Species: HUMAN Name: Desmin
Index: MW: 53536 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
969.4149969.381035pyroGlu 3193250(K)QEMMEYR(H)
986.3366986.4076-723193250(K) (H)
1001.29721001.3708-74pyroGlu 2Met-ox 3193250(K)QEMMEYR(H)
1018.36911018.3974-282Met-ox 3193250(K)QEMMEYR(H)
2369.04322369.1585-494504701(K) (-)
2384.06802384.2172-633874061(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (48/470AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 5/75 matches (6%).
Acc. #: Species: MOUSE Name: Transcobalamin II precursor (TCII) (TC II)
Index: MW: 47586 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1229.47641229.5737-791141231(R) (G)
2020.99062021.0378-2360771(R) (I)
2036.98602037.0327-231Met-ox 60771(R)LSSMQAGTKEDLYLHSLK(I)
2343.04362343.2283-7969861(K) (S)
2705.19682705.3714-653393630(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (62/430AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 5/75 matches (6%).
Acc. #: Species: HUMAN Name: Protein-tyrosine phosphatase epsilon precursor (R-PTP-epsilon)
Index: MW: 80642 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1140.40251140.5108-951281361(R) (E)
1218.57261218.6330-505575670(K) (D)
1949.95661949.9662-4.95896050(R) (G)
2282.28482282.2556133203411(K) (T)
2384.06802384.1531-366676840(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (77/700AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 5/75 matches (6%).
Acc. #: Species: HUMAN Name: Nucleolar autoantigen No55
Index: MW: 50382 Da pI: 4.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
954.3538954.3838-322222290(R) (A)
970.4003970.3788221Met-ox 2222290(R)SSTEDMER(A)
1163.40281163.5155-97961040(R) (L)
1229.47641229.5876-903153250(R) (D)
1515.75481515.7266193263370(K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (40/437AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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