MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D34
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1169 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/100(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.696e+01014 (14)47.014.019.934.8 47269/4.9HUMANGamma enolase (2-phospho-D-glycerate hydro-lyase) (Neural enolase) (NSE) (Enolase 2)
28.140e+00610 (10)34.010.017.536.3 47297/5.0MOUSEGamma enolase (2-phospho-D-glycerate hydro-lyase) (Neural enolase) (NSE) (Enolase 2)
31.035e+00610 (10)24.010.011.041.0 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
41.118e+0057 (7)17.07.0-1.7031.5 59519/5.1HUMANKeratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
52.759e+0047 (7)12.07.0-1.9053.2 95953/5.4MOUSEMajor vault protein (MVP)
106277..............................................................x..x.xxx......x.x.....x.x...x..x.xxx..
90529..............................................................x....x.x......x.x.....x.x...x..x...x..
46726...........................................x.....xx....x..x.......x..x...x..............x.....x.....
111704.................................x......x.............x...................x....x.............x.....x
61474....................................x................x..........x.......x.......x........x.......x..



Detailed Results

1. 14/100 matches (14%).
Acc. #: Species: HUMAN Name: Gamma enolase (2-phospho-D-glycerate hydro-lyase) (Neural enolase) (NSE) (Enolase 2)
Index: MW: 47269 Da pI: 4.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1533.83411533.8463-8.01061200(K) (A)
1570.82021570.7939172402530(K) (D)
1583.75661583.7753-123593720(K) (S)
1586.80361586.78899.31Met-ox 2402530(K)IVIGMDVAASEFYR(D)
1599.77781599.77024.81Met-ox 3593720(K)LAQENGWGVMVSHR(S)
1804.97331804.94451633500(R) (D)
1858.95631858.9339122702850(R) (D)
1955.04801954.9771361Met-ox 1631790(K)LAMQEFMILPVGAESFR(D)
1971.01491970.9720222Met-ox 1631790(K)LAMQEFMILPVGAESFR(D)
2102.17952102.1134313073260(K) (R)
2367.25792367.1754353733940(R) (T)
2483.18652483.1043332863060(R) (F)
2578.48852578.37284565890(K) (L)
2702.41702702.3161372032280(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 47% (208/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/100 matches (10%).
Acc. #: Species: MOUSE Name: Gamma enolase (2-phospho-D-glycerate hydro-lyase) (Neural enolase) (NSE) (Enolase 2)
Index: MW: 47297 Da pI: 5.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1533.83411533.8463-8.01061200(K) (A)
1583.75661583.7753-123593720(K) (S)
1599.77781599.77024.81Met-ox 3593720(K)LAQENGWGVMVSHR(S)
1804.97331804.94451633500(R) (D)
1858.95631858.9339122702850(R) (N)
1955.04801954.9771361Met-ox 1631790(K)LAMQEFMILPVGAESFR(D)
1971.01491970.9720222Met-ox 1631790(K)LAMQEFMILPVGAESFR(D)
2102.17952102.1134313073260(K) (R)
2367.25792367.1754353733940(R) (T)
2702.41702702.3161372032280(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (148/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/100 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1179.61961179.6010163773860(K) (H)
1265.64361265.63784.62782880(R) (K)
1277.71501277.71053.54734830(K) (T)
1383.69971383.69096.41861970(K) (V)
1475.77601475.7494182122230(K) (T)
1475.77601475.7858-6.62002110(R) (W)
1574.72531574.6579432Met-ox 2582691(K)NMQDMVEDYRNK(Y)
1599.77781599.8342-354424551(K) (E)
1657.84151657.79342913290(R) (R)
1994.02161993.9772222242390(R) (R)
2384.00162383.9524215195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (158/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/100 matches (7%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1090.51291090.5315-171481560(K) (L)
1165.60141165.5853144424500(R) (S)
1365.63021365.6399-7.13233330(R) (K)
1707.81461707.77272541590(K) (G)
1880.91951880.9540-181481631(K) (V)
2367.25792367.2632-2.32082280(K) (L)
2872.37752872.3933-5.52963220(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (103/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/100 matches (7%).
Acc. #: Species: MOUSE Name: Major vault protein (MVP)
Index: MW: 95953 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1130.55921130.6057-417357450(R) (L)
1329.65461329.6738-1457670(R) (D)
1549.76761549.8048-247958081(K) (D)
1615.80611615.7613282802930(R) (N)
1890.99921891.0037-2.468841(R) (H)
2083.10402083.0422304004170(R) (E)
2702.41702702.3864115135360(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (109/861AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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