MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D34
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1158 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/56(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.205e+00616 (28)42.028.652.060.8 48106/5.0HUMANKeratin, type I cytoskeletal 17 (Cytokeratin 17) (K17) (CK 17) (39.1)
22.439e+0049 (16)20.016.128.0127 65866/8.1HUMANKeratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
392277 (12)11.012.535.890.6 82673/6.3HUMANDiacylglycerol kinase, alpha (Diglyceride kinase) (DGK-alpha) (DAG kinase alpha) (80 kDa diacylglycerol kinase)
471418 (14)11.014.348.2138 83269/7.2HUMANComplement C2 precursor (C3/C5 convertase)
5615810 (17)12.017.918.893.1 93868/7.0HUMANHYPOTHETICAL PROTEIN KIAA0184
9067xx.......x.x..x..x......x.....x.x......x..xx.x..x.xx....
114359.x......x............x......x..x....xx......x..x........
81750........x...x.x.............x..............x....x..x....
96636.....x..............x..x.......x..x.....x......x.....x..
112504.x....x......x...............x.xx...x....x...xx.........



Detailed Results

1. 16/56 matches (28%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 17 (Cytokeratin 17) (K17) (CK 17) (39.1)
Index: MW: 48106 Da pI: 5.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
993.5509993.5005511731800(K) (L)
993.5509993.5621-112132201(K) (N)
994.4946994.4270681301360(R) (T)
1029.64801029.5944521932010(R) (A)
1036.59631036.5328611161221(K) (Q)
1036.59631036.5427521641721(R) (F)
1048.57771048.52105431410(R) (L)
1064.58311064.6104-26951031(R) (A)
1144.58971144.4951832702780(K) (T)
1185.70851185.6955111922011(R) (A)
1222.71831222.6432611711801(R) (L)
1342.83331342.7443661811921(R) (V)
1379.81851379.7283653753851(K) (R)
1403.80301403.6767903223340(K) (Y)
1516.85341516.7647583873990(R) (K)
1882.04501881.9302612Met-ox 3063210(R)TMQALEIELQSQLSMK(A)
2068.29762068.1402761451630(K) (L)
2130.14672130.0137621Met-ox 2312510(R)GQVGGEINVEMDAAPGVDLSR(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (182/432AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/56 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
994.4946994.5686-744124200(R) (K)
1019.55931019.4832754894960(K) (M)
1084.40321084.4985-881Met-ox 2943020(K)DVDNAYMIK(V)
1176.51651176.5537-323713800(K) (Y)
1193.69341193.6166643813900(K) (H)
1193.69341193.6279552442531(K) (T)
1320.66391320.58346146610(R) (S)
1329.74801329.6399814254360(K) (G)
1329.74801329.7266163483590(R) (A)
1475.88591475.7858682042150(R) (W)
1839.06561838.91498271920(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (130/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/56 matches (12%).
Acc. #: Species: HUMAN Name: Diacylglycerol kinase, alpha (Diglyceride kinase) (DGK-alpha) (DAG kinase alpha) (80 kDa diacylglycerol kinase)
Index: MW: 82673 Da pI: 6.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1019.55931019.4838747277350(R) (-)
1040.53121040.5165145445511(K) (M)
1048.57771048.4985766446520(K) (R)
1176.51651176.5748-501271370(R) (I)
1403.80301403.7535356180(R) (Y)
1882.04501881.9935276176340(R) (V)
2130.14672129.9894745565720(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (85/735AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/56 matches (14%).
Acc. #: Species: HUMAN Name: Complement C2 precursor (C3/C5 convertase)
Index: MW: 83269 Da pI: 7.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1007.58111007.4832972752830(K) (I)
1080.54771080.6417-873693780(R) (S)
1111.64791111.5384996026100(R) (Q)
1193.69341193.5989796476560(K) (E)
1277.78331277.6602966776881(K) (F)
1365.71491365.7531-287417520(R) (-)
1839.06561838.9798473693861(R) (T)
2225.30882225.1243834034211(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (89/752AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/56 matches (17%).
Acc. #: Species: HUMAN Name: HYPOTHETICAL PROTEIN KIAA0184
Index: MW: 93868 Da pI: 7.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
994.4946994.5573-635805880(R) (L)
1011.53861011.5839-456626700(K) (G)
1045.60321045.5828203213301(R) (L)
1179.66581179.5832704734810(K) (Q)
1193.69341193.6135671Met-ox 5595681(R)MKGVNLSCVR(T)
1222.71831222.6796321952060(K) (Q)
1320.66391320.6946-233883990(K) (L)
1377.71791377.6619411Met-ox 5695790(R)TCMVVAEERPR(I)
1516.85341516.7794493033160(K) (R)
1535.93771535.8738422752861(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (108/863AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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