MS-Fit Search Results

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D36
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1256 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/86(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	1.300e+011	17 (19)	52.0	19.8	-54.3	80.4	45519/5.0	HUMAN	cAMP-dependent protein kinase type II-alpha regulatory chain
2	2.536e+006	10 (11)	13.0	11.6	-39.9	101	81866/7.7	HUMAN	Protein kinase C, theta type (nPKC-theta)
3	1.311e+006	13 (15)	40.0	15.1	-60.4	38.9	47790/4.9	MOUSE	Histone acetyltransferase type B subunit 2 (Retinoblastoma binding protein p46) (Retinoblastoma-binding protein 7) (RBBP-7)
4	1.310e+006	13 (15)	40.0	15.1	-60.4	38.9	47820/4.9	HUMAN	Histone acetyltransferase type B subunit 2 (Retinoblastoma binding protein P46) (Retinoblastoma-binding protein 7) (RBBP-7)
5	1.252e+005	8 (9)	17.0	9.3	-61.0	40.3	45389/4.8	MOUSE	CAMP-DEPENDENT PROTEIN KINASE TYPE II-ALPHA REGULATORY CHAIN

66232	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	.	.	.	x	.	x	x	.	.	.	.	x	.	x	.	.	x	x	.	x	x	.	x	x	.	.	.	.	.	.
88077	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	x	x	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.
46719	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	x	.	x	x	.
23694	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	x	.	x	x	.
38132	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	x	x	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 17/86 matches (19%).
Acc. #: Species: HUMAN Name: cAMP-dependent protein kinase type II-alpha regulatory chain
Index: MW: 45519 Da pI: 5.0

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
938.3308	938.4332	-109		196	203	0	(R) (G)
978.3953	978.4427	-48		123	129	1	(K) (L)
1051.6318	1051.5424	85		348	358	0	(R) (C)
1245.5009	1245.6439	-115		284	294	1	(K) (A)
1381.5769	1381.6608	-61		359	369	0	(K) (L)
1397.5742	1397.6557	-58	1Met-ox	359	369	0	(K)CLVMDVQAFER(L)
1483.6384	1483.7294	-61		57	70	0	(R) (V)
1555.6847	1555.7579	-47		204	217	0	(R) (A)
1555.6847	1555.8082	-79	1Met-ox	251	263	0	(K)MFESFIESVPLLK(S)
1571.6691	1571.7528	-53	1Met-ox	204	217	0	(R)GSFGELALMYNTPR(A)
1692.8218	1692.9073	-51		333	347	0	(K) (A)
1746.8033	1746.9026	-57		218	234	0	(R) (V)
1911.8903	1912.0068	-61		295	311	0	(K) (S)
1938.8662	1938.9602	-48		24	39	0	(R) (L)
2035.8730	2035.9759	-51		142	158	0	(K) (I)
2051.8944	2051.9708	-37	1Met-ox	142	158	0	(K)NLDQEQLSQVLDAMFER(I)
2306.8806	2307.0166	-59		162	181	0	(K) (G)
2505.2058	2505.3717	-66	AcetN	1	23	0	(-)SHIQIPPGLTELLQGYTVEVLR(Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 52% (214/404AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/86 matches (11%).
Acc. #: Species: HUMAN Name: Protein kinase C, theta type (nPKC-theta)
Index: MW: 81866 Da pI: 7.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
968.4234 968.5205 -100 402 409 0 (K) (A)
1039.4849 1039.4671 17 593 600 0 (R) (W)
1130.5496 1130.5992 -44 277 286 0 (K) (L)
1133.3905 1133.5189 -113 121 129 0 (R) (D)
1381.5769 1381.6608 -61 356 366 0 (K) (E)
1397.5742 1397.6557 -58 1Met-ox 356 366 0 (K)MCHLPEPELNK(E)
1439.7047 1439.6775 19 681 692 0 (R) (N)
1471.6529 1471.6674 -9.8 2Met-ox 681 692 0 (R)ALINSMDQNMFR(N)
1911.8903 1911.8390 27 414 429 0 (K) (R)
2051.8944 2052.0501 -76 78 95 0 (K) (C)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Database Sequence
968.4234	968.5205	-100		402	409	(K) (A)
1039.4849	1039.4671	17		593	600	(R) (W)
1130.5496	1130.5992	-44		277	286	(K) (L)
1133.3905	1133.5189	-113		121	129	(R) (D)
1381.5769	1381.6608	-61		356	366	(K) (E)
1397.5742	1397.6557	-58	1Met-ox	356	366	(K)MCHLPEPELNK(E)
1439.7047	1439.6775	19		681	692	(R) (N)
1471.6529	1471.6674	-9.8	2Met-ox	681	692	(R)ALINSMDQNMFR(N)
1911.8903	1911.8390	27		414	429	(K) (R)
2051.8944	2052.0501	-76		78	95	(K) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (92/706AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 13/86 matches (15%).
Acc. #: Species: MOUSE Name: Histone acetyltransferase type B subunit 2 (Retinoblastoma binding protein p46) (Retinoblastoma-binding protein 7) (RBBP-7)
Index: MW: 47790 Da pI: 4.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
934.4170 934.4820 -70 251 257 0 (K) (S)
950.4047 950.4770 -76 1Met-ox 251 257 0 (K)LMIWDTR(S)
973.4822 973.5471 -67 296 303 0 (K) (N)
1067.4088 1067.5234 -107 120 128 0 (K) (A)
1130.5496 1130.6322 -73 340 348 1 (R) (I)
1428.6409 1428.7309 -63 1Met-ox 131 142 0 (R)YMPQNPHIIATK(T)
1471.6529 1471.7321 -54 143 155 0 (K) (H)
1628.6361 1628.7114 -46 AcetN 1Met-ox 1 14 1 (-)ASKEMFEDTVEER(V)
1803.7440 1803.8448 -56 156 171 0 (K) (L)
2774.2671 2774.4014 -48 59 82 0 (K) (V)
2847.1810 2847.3437 -57 349 375 0 (K) (I)
3256.4419 3256.5585 -36 143 171 1 (K) (L)
3308.3478 3308.4555 -33 83 113 1 (R) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
934.4170	934.4820	-70		251	257	0	(K) (S)
950.4047	950.4770	-76	1Met-ox	251	257	0	(K)LMIWDTR(S)
973.4822	973.5471	-67		296	303	0	(K) (N)
1067.4088	1067.5234	-107		120	128	0	(K) (A)
1130.5496	1130.6322	-73		340	348	1	(R) (I)
1428.6409	1428.7309	-63	1Met-ox	131	142	0	(R)YMPQNPHIIATK(T)
1471.6529	1471.7321	-54		143	155	0	(K) (H)
1628.6361	1628.7114	-46	AcetN 1Met-ox	1	14	1	(-)ASKEMFEDTVEER(V)
1803.7440	1803.8448	-56		156	171	0	(K) (L)
2774.2671	2774.4014	-48		59	82	0	(K) (V)
2847.1810	2847.3437	-57		349	375	0	(K) (I)
3256.4419	3256.5585	-36		143	171	1	(K) (L)
3308.3478	3308.4555	-33		83	113	1	(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (170/425AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/86 matches (15%).
Acc. #: Species: HUMAN Name: Histone acetyltransferase type B subunit 2 (Retinoblastoma binding protein P46) (Retinoblastoma-binding protein 7) (RBBP-7)
Index: MW: 47820 Da pI: 4.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
934.4170 934.4820 -70 251 257 0 (K) (S)
950.4047 950.4770 -76 1Met-ox 251 257 0 (K)LMIWDTR(S)
973.4822 973.5471 -67 296 303 0 (K) (N)
1067.4088 1067.5234 -107 120 128 0 (K) (A)
1130.5496 1130.6322 -73 340 348 1 (R) (I)
1428.6409 1428.7309 -63 1Met-ox 131 142 0 (R)YMPQNPHIIATK(T)
1471.6529 1471.7321 -54 143 155 0 (K) (H)
1628.6361 1628.7114 -46 AcetN 1Met-ox 1 14 1 (-)ASKEMFEDTVEER(V)
1803.7440 1803.8448 -56 156 171 0 (K) (L)
2774.2671 2774.4014 -48 59 82 0 (K) (V)
2847.1810 2847.3437 -57 349 375 0 (K) (I)
3256.4419 3256.5585 -36 143 171 1 (K) (L)
3308.3478 3308.4555 -33 83 113 1 (R) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
934.4170	934.4820	-70		251	257	0	(K) (S)
950.4047	950.4770	-76	1Met-ox	251	257	0	(K)LMIWDTR(S)
973.4822	973.5471	-67		296	303	0	(K) (N)
1067.4088	1067.5234	-107		120	128	0	(K) (A)
1130.5496	1130.6322	-73		340	348	1	(R) (I)
1428.6409	1428.7309	-63	1Met-ox	131	142	0	(R)YMPQNPHIIATK(T)
1471.6529	1471.7321	-54		143	155	0	(K) (H)
1628.6361	1628.7114	-46	AcetN 1Met-ox	1	14	1	(-)ASKEMFEDTVEER(V)
1803.7440	1803.8448	-56		156	171	0	(K) (L)
2774.2671	2774.4014	-48		59	82	0	(K) (V)
2847.1810	2847.3437	-57		349	375	0	(K) (I)
3256.4419	3256.5585	-36		143	171	1	(K) (L)
3308.3478	3308.4555	-33		83	113	1	(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (170/425AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/86 matches (9%).
Acc. #: Species: MOUSE Name: CAMP-DEPENDENT PROTEIN KINASE TYPE II-ALPHA REGULATORY CHAIN
Index: MW: 45389 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
938.3308 938.4332 -109 193 200 0 (R) (G)
978.3953 978.4427 -48 120 126 1 (K) (L)
1381.5769 1381.6608 -61 356 366 0 (K) (L)
1397.5742 1397.6557 -58 1Met-ox 356 366 0 (K)CLVMDVQAFER(L)
1555.6847 1555.7579 -47 201 214 0 (R) (A)
1571.6691 1571.7528 -53 1Met-ox 201 214 0 (R)GSFGELALMYNTPR(A)
1692.8218 1692.9073 -51 330 344 0 (K) (A)
1911.8903 1912.0068 -61 292 308 0 (K) (S)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
938.3308	938.4332	-109		193	200	0	(R) (G)
978.3953	978.4427	-48		120	126	1	(K) (L)
1381.5769	1381.6608	-61		356	366	0	(K) (L)
1397.5742	1397.6557	-58	1Met-ox	356	366	0	(K)CLVMDVQAFER(L)
1555.6847	1555.7579	-47		201	214	0	(R) (A)
1571.6691	1571.7528	-53	1Met-ox	201	214	0	(R)GSFGELALMYNTPR(A)
1692.8218	1692.9073	-51		330	344	0	(K) (A)
1911.8903	1912.0068	-61		292	308	0	(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (72/401AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):