MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D38
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 2256 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/124(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.498e+00512 (9)12.09.7-10.560.8 96697/6.4HUMANGlycogen phosphorylase, brain form
22.168e+00513 (10)10.010.5-7.4464.6 96985/6.9HUMANPhosphatidylinositol 4-kinase alpha (PI4-kinase) (PtdIns-4-kinase) (PI4K-alpha)
38.216e+00411 (8)11.08.9-24.938.5 92962/9.2HUMANP300/CBP-associated factor (P/CAF) (Histone acetylase PCAF)
45.717e+00410 (8)17.08.1-12.655.1 52248/4.9HUMANKeratin, type I cuticular HA6 (Hair keratin, type I HA6)
55.134e+00412 (9)19.09.7-12.251.9 50320/4.8HUMANKeratin, type I cuticular HA2 (Hair keratin, type I HA2)
62.847e+0048 (6)15.06.5-11.559.7 53686/5.1HUMANVimentin
72.847e+0049 (7)19.07.3-8.5259.5 53688/5.1MOUSEVimentin
82.261e+00412 (9)19.09.7-9.2948.5 81041/10.3HUMANEosinophil peroxidase precursor (EPO)
92.208e+0049 (7)11.07.3-12.258.9 76992/6.5HUMANVillin-like protein
101.957e+0049 (7)10.07.3-16.846.6 90673/9.4HUMANZinc finger protein ZFD25
43800...............x..x......................x.....x..x..xx...........x............................x.....x....xx................
30768.........x.........x..x.................................x...x..x.......................x....x.x..x.xx.........x.............
99052.........................xx..x.....x..........................xx.x...............................x..x........x...x..........
62980.x.........x............................x.......x.x......................x.x.....x...xx.....................................
70849.x.....x....................x...................x.x..xx..................x................xx....................xx..........
54391...............x.........x.x................................x..x...x......................xx................................
112744...............x.........x.x................................x..x...x......................xx.....x..........................
76649..x.......x.....................x................x......x....................x...x....x..........x......x.........x.......x.
65239x.....x...........................x.........x.....................x...................x........x.x..x.......................
44800..x...........................x..x.x...........x.......x..............x.x.............x.....................................


Detailed Results

1. 12/124 matches (9%).
Acc. #: Species: HUMAN Name: Glycogen phosphorylase, brain form
Index: MW: 96697 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1023.47411023.4974-233883950(R) (L)
1045.49181045.5166-247167240(R) (K)
1124.54171124.51252671780(R) (D)
1151.60901151.6438-3062701(R) (T)
1169.54021169.53663.14714790(K) (F)
1181.54171181.5182204294380(R) (R)
1197.53891197.5131221Met-ox 4294380(R)MSVIEEGDCK(R)
1306.57961306.6319-407317400(R) (Q)
1534.73431534.7800-302362481(K) (A)
1625.82631625.7634393213331(R) (V)
1840.85201840.9234-392792931(R) (L)
1867.80541867.8979-507267401(K) (Q)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 12% (109/843AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/124 matches (10%).
Acc. #: Species: HUMAN Name: Phosphatidylinositol 4-kinase alpha (PI4-kinase) (PtdIns-4-kinase) (PI4K-alpha)
Index: MW: 96985 Da pI: 6.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
997.4332997.4413-8.11Met-ox 8138200(R)FSPNMTER(E)
1047.48321047.4933-9.71Met-ox 4154221(R)YDKMGYVR(E)
1063.54431063.5940-475946020(K) (V)
1219.64311219.6720-245115211(K) (V)
1254.57241254.6305-462302391(R) (R)
1270.58261270.6254-341Met-ox 2302391(R)GSQLHKYYMK(R)
1478.69801478.7603-423143261(R) (L)
1517.68941517.68810.853150(R) (F)
1526.61641526.6262-6.4pyroGlu 6636740(R)QTDFGMYDYFTR(Q)
1543.72121543.6528446636740(R) (Q)
1549.67171549.6779-4.02Met-ox 3150(R)EMAGAWHMTVEQK(F)
1559.72061559.6477471Met-ox 6636740(R)QTDFGMYDYFTR(Q)
2113.01322112.9449326586741(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (89/854AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/124 matches (8%).
Acc. #: Species: HUMAN Name: P300/CBP-associated factor (P/CAF) (Histone acetylase PCAF)
Index: MW: 92962 Da pI: 9.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1088.49961088.5336-314824901(R) (R)
1090.52351090.5613-35pyroGlu 1851920(K)QVYFYLFK(L)
1095.47281095.4958-212702790(R) (E)
1107.53711107.5879-461851920(K) (L)
1263.66201263.6632-0.925515611(K) (M)
1270.58261270.6367-435845940(K) (E)
1286.58151286.6316-391Met-ox 5845940(K)GYGTHLMNHLK(E)
1543.72121543.7368-107417530(K) (R)
1559.72061559.7317-7.11Met-ox 7417530(K)SHQSAWPFMEPVK(R)
2028.91382029.0105-487557711(R) (T)
2191.10132191.08716.51Met-ox 2502661(K)MFLNRINYWHLEAPSQR(R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (96/832AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/124 matches (8%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA6 (Hair keratin, type I HA6)
Index: MW: 52248 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
952.4877952.4484411Met-ox 2222280(K)EELMCLK(K)
1008.46701008.5002-331461530(K) (I)
1114.50461114.5203-143954030(R) (L)
1153.52021153.5312-9.6951030(K) (L)
1169.54021169.5261121Met-ox 951030(K)ETMQFLNDR(L)
1383.70381383.7708-481902011(R) (I)
1419.77051419.75967.739530(R) (S)
1459.67831459.7433-45pyroGlu 3723830(R)QNQEYQVLLDVK(A)
1473.69121473.7298-261131241(R) (I)
1476.75251476.7698-123723830(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (84/467AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 12/124 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA2 (Hair keratin, type I HA2)
Index: MW: 50320 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
952.4877952.4484411Met-ox 2252310(K)EELMCLK(K)
982.4803982.4668141851920(K) (Q)
1094.48471094.5040-183984060(R) (L)
1153.52021153.5312-9.6981060(K) (L)
1169.54021169.5261121Met-ox 981060(K)ETMQFLNDR(L)
1169.54021169.5551-132332420(K) (C)
1181.54171181.5989-481831921(R) (Q)
1197.53891197.5938-461Met-ox 1831921(R)AKYEAELAMR(Q)
1383.70381383.7708-481932041(R) (I)
1487.72291487.7494-18pyroGlu 3753860(R)QNQEYQVLLDVR(A)
1504.75211504.7760-163753860(R) (A)
2157.96922157.9731-1.81Met-ox 2642801(R)VLEEMRCQYEAMVEANR(R)
2191.10132191.1181-7.71852031(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (86/448AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/124 matches (6%).
Acc. #: Species: HUMAN Name: Vimentin
Index: MW: 53686 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1023.47411023.5111-362742820(R) (N)
1088.49961088.5336-312082170(R) (L)
1093.48871093.5278-362953040(K) (N)
1254.57241254.56763.81461550(R) (E)
1270.58261270.5625161Met-ox 1461550(R)LGDLYEEEMR(E)
1270.58261270.6392-45pyroGlu 1601701(R)QVDQLTNDKAR(V)
1320.69571320.6330473353451(K) (E)
1487.72291487.7416-13pyroGlu 3223340(R)QVQSLTCEVDALK(G)
1504.75211504.7681-113223340(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (74/466AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/124 matches (7%).
Acc. #: Species: MOUSE Name: Vimentin
Index: MW: 53688 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1023.47411023.5111-362742820(R) (N)
1088.49961088.5336-312082170(R) (L)
1093.48871093.5278-362953040(K) (N)
1254.57241254.56763.81461550(R) (E)
1270.58261270.5625161Met-ox 1461550(R)LGDLYEEEMR(E)
1270.58261270.6392-45pyroGlu 1601701(R)QVDQLTNDKAR(V)
1320.69571320.6330473353451(K) (E)
1487.72291487.7416-13pyroGlu 3223340(R)QVQSLTCEVDALK(G)
1504.75211504.7681-113223340(R) (G)
1543.72121543.6923191Met-ox 14280(R)MFGGSGTSSRPSSNR(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (89/466AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 12/124 matches (9%).
Acc. #: Species: HUMAN Name: Eosinophil peroxidase precursor (EPO)
Index: MW: 81041 Da pI: 10.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
968.4868968.4664212802860(R) (S)
1002.44611002.4679-222872950(R) (N)
1099.56371099.5649-1.04054131(R) (L)
1163.53531163.5883-463683770(R) (S)
1219.64311219.63705.06977061(R) (L)
1441.73251441.7089165655761(R) (F)
1459.67831459.6851-4.7pyroGlu 1Met-ox 5325431(R)QDAMLVDELRDR(L)
1459.67831459.7442-452562670(R) (I)
1476.75251476.7117281Met-ox 5325431(R)QDAMLVDELRDR(L)
1543.72121543.71901.42Met-ox 4724830(R)FGHTMLQPFMFR(L)
1788.83621788.9027-371251401(R) (C)
2282.15822282.1967-17891081(R) (S)
2679.25582679.3097-202452671(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (140/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 9/124 matches (7%).
Acc. #: Species: HUMAN Name: Villin-like protein
Index: MW: 76992 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
951.4655951.4940-3083890(R) (G)
979.4817979.47506.85405470(K) (E)
1104.51511104.5334-17190(-) (T)
1136.56361136.5233362Met-ox 190(-)MMIQWNGPK(T)
1136.56361136.6104-4183911(R) (D)
1306.57961306.5875-6.15916020(K) (S)
1476.75251476.74813.01Met-ox 2092221(K)LAAQLRMVDDGSGK(V)
1534.73431534.7000226646751(K) (Q)
1543.72121543.7976-5046590(K) (R)
1559.72061559.7926-461Met-ox 46590(K)APDLMQIMEAVLGR(R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (78/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 9/124 matches (7%).
Acc. #: Species: HUMAN Name: Zinc finger protein ZFD25
Index: MW: 90673 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
968.4868968.5066-20981051(R) (C)
1096.47031096.4846-1350580(K) (G)
1102.56901102.5897-197137210(K) (C)
1107.53711107.5798-392802890(R) (Q)
1151.60901151.5809241681770(K) (H)
1208.60571208.6639-481801890(K) (C)
1348.59761348.6497-394484590(R) (K)
1375.65651375.6606-3.03643750(K) (K)
1476.75251476.74475.34484601(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (81/783AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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