MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D50
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 169 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/38(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.284e+005	13 (34)	41.0	34.2	11.0	29.8		28522/4.7		HUMAN	Tropomyosin alpha 4 chain (Tropomyosin 4) (TM30p1)
2	2431	5 (13)	14.0	13.2	15.3	33.0		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
3	1057	5 (13)	8.0	13.2	12.6	65.6		70188/5.7		MOUSE	Dopamine beta-monooxygenase precursor (Dopamine beta-hydroxylase) (DBH)
4	477	6 (15)	9.0	15.8	26.8	40.6		91800/5.5		MOUSE	HYALURONAN MEDIATED MOTILITY RECEPTOR (INTRACELLULAR HYALURONIC ACID BINDING PROTEIN) (RECEPTOR FOR HYALURONAN-MEDIATED MOTILITY)
5	472	7 (18)	10.0	18.4	1.99	34.8		86994/8.4		HUMAN	Relaxin receptor 1 (Leucine-rich repeat-containing G protein-coupled receptor 7)

47680	.	.	x	.	x	.	x	.	x	x	.	x	.	.	x	.	.	.	x	.	x	.	.	.	x	.	x	.	.	.	x	.	.	.	.	.	x	.
46726	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x
83919	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	x	.	.	.	.	.	.	.	x	.	.
97177	.	.	.	.	.	.	x	.	.	x	.	x	.	.	.	.	.	.	.	.	x	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.
64126	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	x	.

Detailed Results

1. 13/38 matches (34%).
Acc. #: Species: HUMAN Name: Tropomyosin alpha 4 chain (Tropomyosin 4) (TM30p1)
Index: MW: 28522 Da pI: 4.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1043.5574	1043.5737	-16	AcetN	1	11	0	(-)AGLNSLEAVK(R)
1170.6688	1170.6734	-3.9		133	142	0	(K) (A)
1199.6772	1199.6748	2.0	AcetN	1	12	1	(-)AGLNSLEAVKR(K)
1243.6595	1243.6534	4.9		56	65	0	(R) (A)
1272.6766	1272.6548	17		43	54	1	(R) (R)
1298.7797	1298.7684	8.7		132	142	1	(R) (A)
1443.7985	1443.8059	-5.1		70	82	1	(R) (A)
1614.7999	1614.7724	17		14	27	0	(K) (A)
1655.9073	1655.8968	6.3		133	146	1	(K) (A)
1727.9270	1727.8928	20		56	69	1	(R) (L)
1742.9135	1742.8673	26		13	27	1	(R) (A)
1889.9860	1889.9279	31		154	169	1	(K) (S)
2268.2109	2268.1333	34		14	33	1	(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (103/248AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/38 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1179.6003 1179.6010 -0.57 377 386 0 (K) (H)
1475.7770 1475.7494 19 212 223 0 (K) (T)
1475.7770 1475.7858 -6.0 200 211 0 (R) (W)
1638.8838 1638.8604 14 186 199 1 (K) (F)
1994.0326 1993.9772 28 224 239 0 (R) (R)
2384.0414 2383.9524 37 519 549 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (95/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/38 matches (13%).
Acc. #: Species: MOUSE Name: Dopamine beta-monooxygenase precursor (Dopamine beta-hydroxylase) (DBH)
Index: MW: 70188 Da pI: 5.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1243.6595 1243.6534 4.9 589 599 0 (K) (C)
1707.8124 1707.8666 -32 pyroGlu 604 620 0 (R)QTQSPANPTVPITTGGR(C)
1724.8928 1724.8931 -0.19 604 620 0 (R) (C)
1784.0226 1783.9376 48 589 603 1 (K) (Q)
2211.1630 2211.0691 42 558 576 1 (K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (51/621AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/38 matches (15%).
Acc. #: Species: MOUSE Name: HYALURONAN MEDIATED MOTILITY RECEPTOR (INTRACELLULAR HYALURONIC ACID BINDING PROTEIN) (RECEPTOR FOR HYALURONAN-MEDIATED MOTILITY)
Index: MW: 91800 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1199.6772 1199.6200 48 665 673 0 (R) (T)
1272.6766 1272.6687 6.2 253 263 0 (K) (E)
1298.7797 1298.7320 37 380 391 0 (K) (E)
1655.9073 1655.8492 35 538 552 1 (R) (E)
1707.8124 1707.7471 38 781 794 1 (K) (-)
1742.9135 1742.9190 -3.1 707 721 1 (R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (76/794AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/38 matches (18%).
Acc. #: Species: HUMAN Name: Relaxin receptor 1 (Leucine-rich repeat-containing G protein-coupled receptor 7)
Index: MW: 86994 Da pI: 8.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1170.6688 1170.6523 14 162 171 0 (K) (G)
1232.6105 1232.5965 11 695 702 1 (R) (K)
1614.7999 1614.7640 22 690 700 1 (K) (Q)
1638.8838 1638.8604 14 283 296 0 (K) (L)
1884.0388 1884.0595 -11 162 178 1 (K) (L)
1940.9850 1941.0243 -20 1Met-ox 362 376 1 (R)MFRPLMNLSHIYFKK(F)
2268.2109 2268.2491 -17 297 316 1 (K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (79/757AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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