MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D052
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 341 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/61(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.404e+0049 (14)22.014.829.671.0 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
21.891e+00411 (18)20.018.016.954.0 73141/6.7MOUSEPutative ATP-dependent RNA helicase PL10
3759410 (16)17.016.48.0452.2 73244/6.7HUMANDEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
423679 (14)16.014.810.952.0 73102/6.7MOUSEDEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2)
521866 (9)9.09.8-2.3568.5 81211/6.0MOUSEDNA replication licensing factor MCM7 (CDC47 homolog)
48753..............x..x.....................x...x.x..xx.........xx
105637..........x............x..x...x..........x.x......xxxx..x....
3614..........x............x.....xx..........x.x......x.xx..x....
70323..........x............x......x..........x.x......x.xx..x....
75641..............................x..x.........x....x...x......x.



Detailed Results

1. 9/61 matches (14%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1015.41211015.4406-281Met-ox 2902980(K)DVDGAYMTK(V)
1033.47401033.5166-414844920(R) (M)
1179.63191179.6010263773860(K) (H)
1340.73541340.6698493653761(K) (Y)
1475.83411475.7494572122230(K) (T)
1475.83411475.7858332002110(R) (W)
1638.94881638.8604541861991(K) (F)
1657.87641657.79345013290(R) (R)
2300.28832300.1985393443641(R) (S)
2384.08762383.9524575195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (147/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/61 matches (18%).
Acc. #: Species: MOUSE Name: Putative ATP-dependent RNA helicase PL10
Index: MW: 73141 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
989.4836989.43285165730(K) (S)
1051.57751051.5610163333411(R) (Y)
1074.58771074.5472384184260(K) (R)
1093.48501093.48490.131211291(R) (A)
1320.65461320.6800-192762860(R) (K)
1340.73541340.7578-17pyroGlu 2642750(K)QYPISLVLAPTR(E)
1707.86401707.8964-193113251(R) (L)
1765.86311765.7741505996151(R) (A)
1838.98951838.9513215315471(R) (N)
1941.03171940.9792273353501(K) (M)
2083.10352083.0249385115270(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (134/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/61 matches (16%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
989.4836989.43285167750(K) (S)
1051.57751051.5610163343421(R) (Y)
1090.52311090.5421-174194270(K) (R)
1093.48501093.48490.131221301(R) (S)
1320.65461320.6800-192772870(R) (K)
1340.73541340.7578-17pyroGlu 2652760(K)QYPISLVLAPTR(E)
1707.86401707.8964-193123261(R) (L)
1838.98951838.9513215325481(R) (N)
1941.03171940.9792273363511(K) (M)
2083.10352083.0249385125280(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (117/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/61 matches (14%).
Acc. #: Species: MOUSE Name: DEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2)
Index: MW: 73102 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
989.4836989.43285167750(K) (G)
1051.57751051.5610163343421(R) (Y)
1093.48501093.48490.131221301(R) (S)
1320.65461320.6800-192772870(R) (K)
1340.73541340.7578-17pyroGlu 2652760(K)QYPISLVLAPTR(E)
1707.86401707.8964-193123261(R) (L)
1838.98951838.9513215325481(R) (N)
1941.03171940.9792273363511(K) (M)
2083.10352083.0249385125280(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (108/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/61 matches (9%).
Acc. #: Species: MOUSE Name: DNA replication licensing factor MCM7 (CDC47 homolog)
Index: MW: 81211 Da pI: 6.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1093.48501093.4923-6.65625690(R) (Q)
1109.49101109.48723.51Met-ox 5625690(R)YIAMCHER(Q)
1340.73541340.8014-491651761(R) (M)
1638.94881638.9179195005140(R) (F)
1838.98951839.0414-283533700(K) (I)
2300.28832300.1788485155321(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (71/719AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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