MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D054
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 1011 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/80(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.829e+00814 (17)48.017.59.4941.1 29174/4.6HUMAN14-3-3 protein epsilon (Mitochondrial import stimulation factor L subunit) (Protein kinase C inhibitor protein-1) (KCIP-1) (14-3-3E)
21.013e+00511 (13)19.013.86.1037.1 50320/4.8HUMANKeratin, type I cuticular HA2 (Hair keratin, type I HA2)
36.334e+0048 (10)14.010.05.9739.2 47232/4.9HUMANKeratin, type I cuticular HA1 (Hair keratin, type I HA1)
42.727e+0047 (8)19.08.82.6644.1 64587/6.3HUMANMAGUK p55 subfamily member 2 (MPP2 protein) (Discs, large homolog 2)
52.016e+0048 (10)7.010.0-3.7947.6 99077/7.9MOUSESemaphorin 6A precursor (Semaphorin VIA) (Sema VIA) (Semaphorin Q) (Sema Q)
78581.x............xx...x....xx..xx.......x...............x.x.........x........x....x
73974...........xx.x........x..x.......x..x....x.x.............x............x........
103949.........x.xx..x..................x..x.........x............................x...
100604..................x..............x.x...........................xx.......x..x....
1501...x..............x...x.x.....x........................x..x.x...................


Detailed Results

1. 14/80 matches (17%).
Acc. #: Species: HUMAN Name: 14-3-3 protein epsilon (Mitochondrial import stimulation factor L subunit) (Protein kinase C inhibitor protein-1) (KCIP-1) (14-3-3E)
Index: MW: 29174 Da pI: 4.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
917.5045917.5308-2962690(R) (E)
1189.66391189.66152.02162250(K) (D)
1205.66321205.65645.71Met-ox 2162250(K)DSTLIMQLLR(D)
1256.60001256.59117.01311410(R) (K)
1384.69991384.68619.91311421(R) (E)
1393.61571393.6599-32AcetN 1121(-)DDREDLVYQAK(L)
1447.71251447.71031.530420(K) (N)
1463.74551463.7052281Met-ox 30420(K)VAGMDVELTVEER(N)
1504.79411504.775512951060(K) (H)
1819.96161819.9376131541700(K) (L)
1835.97841835.9326251Met-ox 1541700(K)AASDIAMTELPPTHPIR(L)
2088.03532087.9661331972150(K) (D)
2331.23532331.2025141711900(R) (A)
3274.74073274.6041421Met-ox 1972251(K)AAFDDAIAELDTLSEESYKDSTLIMQLLR(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 48% (123/255AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/80 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA2 (Hair keratin, type I HA2)
Index: MW: 50320 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1153.53471153.53123.0981060(K) (L)
1169.54601169.5261171Met-ox 981060(K)ETMQFLNDR(L)
1169.54601169.5551-7.82332420(K) (C)
1189.66391189.6363231571661(K) (M)
1383.73011383.7708-291932041(R) (I)
1400.63441400.5939291Met-ox 2702800(R)CQYEAMVEANR(R)
1487.76851487.749413pyroGlu 3753860(R)QNQEYQVLLDVR(A)
1504.79411504.7760123753860(R) (A)
1540.71271540.70018.22702811(R) (D)
1556.70741556.69508.01Met-ox 2702811(R)CQYEAMVEANRR(D)
1851.94501851.9929-263183330(R) (D)
2191.16852191.1181231852031(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (89/448AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA1 (Hair keratin, type I HA1)
Index: MW: 47232 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1124.51201124.4981124084160(R) (-)
1153.53471153.53123.058660(K) (L)
1169.54601169.5261171Met-ox 58660(K)ETMQFLNDR(L)
1205.66321205.6312271171261(K) (L)
1487.76851487.749413pyroGlu 3353460(R)QNQEYQVLLDVR(A)
1504.79411504.7760123353460(R) (A)
1625.85451625.84992.82092230(R) (V)
2369.09632369.1883-392032231(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (61/416AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/80 matches (8%).
Acc. #: Species: HUMAN Name: MAGUK p55 subfamily member 2 (MPP2 protein) (Discs, large homolog 2)
Index: MW: 64587 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1254.59111254.6079-132112220(K) (A)
1479.74321479.72321372831(K) (L)
1490.74601490.73209.45655760(R) (-)
2070.98832070.97078.55355510(R) (T)
2085.06032084.9790394374541(R) (I)
2211.16762211.1886-9.51701901(K) (I)
2343.12382343.1913-294825010(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (112/576AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/80 matches (10%).
Acc. #: Species: MOUSE Name: Semaphorin 6A precursor (Semaphorin VIA) (Sema VIA) (Semaphorin Q) (Sema Q)
Index: MW: 99077 Da pI: 7.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
968.4848968.48351.31Met-ox 6967041(R)RGSMSSVTK(L)
1254.59111254.6040-102382480(R) (V)
1367.66611367.7224-41pyroGlu 37480(K)QYPVFVGHKPGR(N)
1384.69991384.7490-3537480(K) (N)
1475.77161475.7382238588700(K) (Q)
1835.97841835.9400217908060(K) (A)
1851.94501851.93495.51Met-ox 7908060(K)DMPPMGSPVIPTDLPLR(A)
1985.05941985.04706.337531(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (67/888AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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