MS-Fit Search Results

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D58
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1318 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/70(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	3.140e+011	15 (21)	60.0	21.4	-7.87	40.9	26411/4.7	MOUSE	Proteasome subunit alpha type 5 (Proteasome zeta chain) (Macropain zeta chain) (Multicatalytic endopeptidase complex zeta chain)
2	7.064e+009	13 (18)	47.0	18.6	-8.04	43.8	26469/4.7	HUMAN	Proteasome subunit alpha type 5 (Proteasome zeta chain) (Macropain zeta chain) (Multicatalytic endopeptidase complex zeta chain)
3	6.409e+007	14 (20)	32.0	20.0	-9.50	53.2	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
4	8.467e+004	7 (10)	16.0	10.0	16.0	70.1	77888/8.7	HUMAN	Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
5	7.970e+004	8 (11)	15.0	11.4	-11.9	75.5	72669/8.8	HUMAN	Transcription initiation factor TFIID 70 kDa subunit (TAFII-70) (TAFII-80) (TAFII80)

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Detailed Results

1. 15/70 matches (21%).
Acc. #: Species: MOUSE Name: Proteasome subunit alpha type 5 (Proteasome zeta chain) (Macropain zeta chain) (Multicatalytic endopeptidase complex zeta chain)
Index: MW: 26411 Da pI: 4.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1036.5016	1036.5713	-67	1Met-ox	188	196	1	(K)SMTLKEAIK(S)
1063.4925	1063.5172	-23		11	20	0	(R) (L)
1423.7653	1423.7837	-13		21	32	0	(R) (L)
1431.7081	1431.7405	-23		54	66	0	(R) (I)
1447.7309	1447.7354	-3.1	1Met-ox	54	66	0	(R)ITSPLMEPSSIEK(I)
1603.8353	1603.8365	-0.80	1Met-ox	53	66	1	(K)RITSPLMEPSSIEK(I)
1961.9557	1961.9569	-0.61		169	187	0	(R) (S)
2047.0790	2047.0745	2.2		33	52	0	(K) (R)
2098.0283	2098.0677	-19		67	86	0	(K) (T)
2114.0448	2114.0626	-8.4	1Met-ox	67	86	0	(K)IVEIDAHIGCAMSGLIADAK(T)
2176.9993	2176.9908	3.9		150	168	0	(K) (A)
2193.0033	2192.9857	8.0	1Met-ox	150	168	0	(K)GPQLFHMDPSGTFVQCDAR(A)
2203.1709	2203.1756	-2.1		33	53	1	(K) (I)
2474.2647	2474.2502	5.9		210	231	0	(K) (E)
2490.2996	2490.2451	22	1Met-ox	210	231	0	(K)LNATNIELATVQPGQNFHMFTK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 60% (145/241AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/70 matches (18%).
Acc. #: Species: HUMAN Name: Proteasome subunit alpha type 5 (Proteasome zeta chain) (Macropain zeta chain) (Multicatalytic endopeptidase complex zeta chain)
Index: MW: 26469 Da pI: 4.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1036.5016 1036.5713 -67 1Met-ox 188 196 1 (K)SMTLKEAIK(S)
1063.4925 1063.5172 -23 11 20 0 (R) (L)
1431.7081 1431.7405 -23 54 66 0 (R) (I)
1447.7309 1447.7354 -3.1 1Met-ox 54 66 0 (R)ITSPLMEPSSIEK(I)
1603.8353 1603.8365 -0.80 1Met-ox 53 66 1 (K)RITSPLMEPSSIEK(I)
2047.0790 2047.0745 2.2 33 52 0 (K) (R)
2098.0283 2098.0677 -19 67 86 0 (K) (T)
2114.0448 2114.0626 -8.4 1Met-ox 67 86 0 (K)IVEIDAHIGCAMSGLIADAK(T)
2176.9993 2176.9908 3.9 150 168 0 (K) (A)
2193.0033 2192.9857 8.0 1Met-ox 150 168 0 (K)GPQLFHMDPSGTFVQCDAR(A)
2203.1709 2203.1756 -2.1 33 53 1 (K) (I)
2474.2647 2474.2502 5.9 210 231 0 (K) (E)
2490.2996 2490.2451 22 1Met-ox 210 231 0 (K)LNATNIELATVQPGQNFHMFTK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 47% (114/241AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 14/70 matches (20%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
973.4920 973.5318 -41 396 403 0 (K) (V)
1066.4830 1066.5169 -32 270 277 1 (K) (T)
1179.5771 1179.6010 -20 377 386 0 (K) (H)
1277.6992 1277.6337 51 247 257 1 (K) (N)
1277.6992 1277.7105 -8.8 473 483 0 (K) (T)
1300.5190 1300.5302 -8.6 258 267 0 (K) (N)
1383.6788 1383.6909 -8.7 186 197 0 (K) (V)
1469.7379 1469.8440 -72 396 407 1 (K) (L)
1475.7511 1475.7494 1.1 212 223 0 (K) (T)
1475.7511 1475.7858 -24 200 211 0 (R) (W)
1657.7795 1657.7934 -8.4 13 29 0 (R) (R)
1687.9669 1687.9707 -2.2 66 82 1 (R) (G)
1716.8431 1716.8517 -5.0 418 432 0 (K) (G)
1941.9998 1941.9882 6.0 444 460 1 (K) (L)
1993.9952 1993.9772 9.0 224 239 0 (R) (R)
2383.9787 2383.9524 11 519 549 0 (R) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
973.4920	973.5318	-41	396	403	0	(K) (V)
1066.4830	1066.5169	-32	270	277	1	(K) (T)
1179.5771	1179.6010	-20	377	386	0	(K) (H)
1277.6992	1277.6337	51	247	257	1	(K) (N)
1277.6992	1277.7105	-8.8	473	483	0	(K) (T)
1300.5190	1300.5302	-8.6	258	267	0	(K) (N)
1383.6788	1383.6909	-8.7	186	197	0	(K) (V)
1469.7379	1469.8440	-72	396	407	1	(K) (L)
1475.7511	1475.7494	1.1	212	223	0	(K) (T)
1475.7511	1475.7858	-24	200	211	0	(R) (W)
1657.7795	1657.7934	-8.4	13	29	0	(R) (R)
1687.9669	1687.9707	-2.2	66	82	1	(R) (G)
1716.8431	1716.8517	-5.0	418	432	0	(K) (G)
1941.9998	1941.9882	6.0	444	460	1	(K) (L)
1993.9952	1993.9772	9.0	224	239	0	(R) (R)
2383.9787	2383.9524	11	519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (211/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/70 matches (10%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1431.7081 1431.6327 53 16 26 0 (R) (A)
1460.7527 1460.7246 19 370 382 0 (K) (M)
1519.6857 1519.7691 -55 533 545 0 (K) (V)
2053.1151 2053.0831 16 204 221 1 (K) (I)
2211.0879 2211.0756 5.6 143 161 0 (K) (T)
2225.1053 2225.0304 34 606 624 0 (R) (K)
2284.1630 2284.0715 40 471 489 1 (K) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
1431.7081	1431.6327	53	16	26	0	(R) (A)
1460.7527	1460.7246	19	370	382	0	(K) (M)
1519.6857	1519.7691	-55	533	545	0	(K) (V)
2053.1151	2053.0831	16	204	221	1	(K) (I)
2211.0879	2211.0756	5.6	143	161	0	(K) (T)
2225.1053	2225.0304	34	606	624	0	(R) (K)
2284.1630	2284.0715	40	471	489	1	(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (112/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/70 matches (11%).
Acc. #: Species: HUMAN Name: Transcription initiation factor TFIID 70 kDa subunit (TAFII-70) (TAFII-80) (TAFII80)
Index: MW: 72669 Da pI: 8.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1090.5013 1090.5493 -44 180 191 1 (K) (G)
1090.5013 1090.5493 -44 182 193 1 (K) (E)
1405.6662 1405.6824 -12 343 354 0 (K) (I)
1405.6662 1405.7626 -69 331 342 1 (R) (H)
1407.6992 1407.7194 -14 288 299 0 (K) (Y)
1423.7653 1423.7143 36 1Met-ox 288 299 0 (K)ALMDNPTLYLEK(Y)
1431.7081 1431.6756 23 pyroGlu 648 662 0 (K)QEAGDSPPPAPGTPK(A)
1448.7584 1448.7021 39 648 662 0 (K) (A)
2064.0552 2064.0343 10 79 95 0 (K) (F)
2474.2647 2474.3731 -44 480 502 0 (R) (T)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1090.5013	1090.5493	-44		180	191	1	(K) (G)
1090.5013	1090.5493	-44		182	193	1	(K) (E)
1405.6662	1405.6824	-12		343	354	0	(K) (I)
1405.6662	1405.7626	-69		331	342	1	(R) (H)
1407.6992	1407.7194	-14		288	299	0	(K) (Y)
1423.7653	1423.7143	36	1Met-ox	288	299	0	(K)ALMDNPTLYLEK(Y)
1431.7081	1431.6756	23	pyroGlu	648	662	0	(K)QEAGDSPPPAPGTPK(A)
1448.7584	1448.7021	39		648	662	0	(K) (A)
2064.0552	2064.0343	10		79	95	0	(K) (F)
2474.2647	2474.3731	-44		480	502	0	(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (105/677AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):