MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D72
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1132 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/80(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
18.403e+01121 (26)35.026.33.4843.1 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
28.691e+01021 (26)60.026.3-6.4749.2 35937/4.9HUMANAnnexin V (Lipocortin V) (Endonexin II) (Calphobindin I) (CBP-I) (Placental anticoagulant protein I) (PAP-I) (PP4) (Thromboplastin inhibitor) (Vascular anticoagulant-alpha) (VAC-alpha) (Anchorin CII)
31.655e+01017 (21)37.021.39.6352.0 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
41.980e+00613 (16)34.016.3-4.9743.2 35753/4.8MOUSEAnnexin V (Lipocortin V) (Endonexin II) (Calphobindin I) (CBP-I) (Placental anticoagulant protein I) (PAP-I) (PP4) (Thromboplastin inhibitor) (Vascular anticoagulant-alpha) (VAC-alpha) (Anchorin CII)
52.098e+0048 (10)14.010.011.037.1 62918/8.1HUMANTissue-type plasminogen activator precursor (tPA) (t-PA) (t-plasminogen activator) (Alteplase) (Reteplase)
61.693e+0048 (10)12.010.0-8.9364.6 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
71.205e+0048 (10)20.010.0-11.065.4 59621/5.4HUMANT-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
850386 (7)27.07.511.542.7 25760/8.9MOUSETHYMIDINE KINASE, CYTOSOLIC
942107 (8)13.08.815.559.1 71773/8.6MOUSEZinc finger protein 147 (Tripartite motif protein 25) (Estrogen responsive finger protein) (Efp)
10347210 (12)18.012.5-5.9451.5 79525/8.8MOUSEEstradiol 17 beta-dehydrogenase 4 (17-beta-HSD 4) (17-beta-hydroxysteroid dehydrogenase 4)
45313........x...x.xx.....x.....x..xx.xx..x.xx.x...xxx..x.............x.x...x........
28941x..xx..x...x.xx...x...xx..x..x..x....x.......x...x..xx..x..................xx...
97443.....xx...x.....xx..x...x..........x...................x...x..x.....x.x.xx...xx.
37033...x.......x.xx...x....x..x..x..x............x......xx..x.......................
95716......x.......x............x...x................x...xx...............x..........
42717.......x...................x..........x.........x..x...x......x..x..............
61980.....x...........x.......x..x......x..........................x........x....x...
52302....x............x............................x.....xx...........x..............
88358......................x.....x..........x..........x....x.x..........x...........
66687.........x......x........x.x..x...x..........x........x..x...................x..


Detailed Results

1. 21/80 matches (26%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
832.4711832.4892-2275820(K) (G)
973.5207973.5318-113964030(K) (V)
1014.50211014.4791231Met-ox 181(-)MSRQFSSR(S)
1033.51231033.5166-4.24844920(R) (M)
1141.53871141.5199164644720(R) (L)
1179.60361179.60102.23773860(K) (H)
1265.64181265.63783.22782880(R) (K)
1277.72231277.71059.24734830(K) (T)
1300.54441300.5302112582670(K) (N)
1302.65171302.7018-383934031(R) (V)
1302.65171302.7157-493443550(R) (A)
1340.62311340.6698-353653761(K) (Y)
1383.69601383.69093.71861970(K) (V)
1393.76521393.7327232782891(R) (D)
1475.77371475.7494162122230(K) (T)
1475.77371475.7858-8.22002110(R) (W)
1638.89551638.8604211861991(K) (F)
1657.81801657.79341513290(R) (R)
1699.85441699.825117pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1716.89181716.8517234184320(K) (G)
1994.01301993.9772182242390(R) (R)
2150.12412150.0783212242401(R) (R)
2384.00902383.9524245195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (230/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 21/80 matches (26%).
Acc. #: Species: HUMAN Name: Annexin V (Lipocortin V) (Endonexin II) (Calphobindin I) (CBP-I) (Placental anticoagulant protein I) (PAP-I) (PP4) (Thromboplastin inhibitor) (Vascular anticoagulant-alpha) (VAC-alpha) (Anchorin CII)
Index: MW: 35937 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
628.3555628.3782-36AcetN 160(-)AQVLR(G)
698.3671698.3949-402022070(R) (K)
744.3651744.3892-321181230(R) (A)
775.3731775.3950-2819250(R) (K)
954.5282954.5413-141942010(K) (S)
1001.58471001.5995-151091170(K) (T)
1014.50211014.5148-1290970(R) (H)
1106.58121106.5846-3.12772850(R) (K)
1143.63751143.6924-481Met-ox 80890(K)LIVALMKPSR(L)
1155.57721155.57581.22612710(K) (V)
1172.63601172.6825-401Met-ox 1521610(R)MLVVLLQANR(D)
1234.66751234.6796-9.82772861(R) (E)
1290.64631290.64044.61Met-ox 2913010(K)NFATSLYSMIK(G)
1340.62311340.61238.17180(R) (A)
1613.89291613.9114-112282420(R) (S)
1704.92361704.90201330450(K) (S)
1733.93781733.8824322462600(R) (G)
1749.91691749.8773231Met-ox 2462600(R)SIPAYLAETLYYAMK(G)
1799.93271799.8968201872011(K) (S)
2871.28422871.212125pyroGlu 1271510(K)QVYEEEYGSSLEDDVVGDTSGYYQR(M)
2888.31862888.2386281271510(K) (M)

Click link below to search for cysteine linked fragments.

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Click link below to search for another component.

The matched peptides cover 60% (192/320AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 17/80 matches (21%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
745.3871745.4208-453683730(K) (H)
749.3291749.3616-433213260(K) (Q)
897.4048897.4140-102332390(R) (I)
1060.56231060.5639-1.42242320(K) (M)
1065.50341065.49993.31541620(K) (L)
1140.55041140.5649-13pyroGlu 2502600(R)QGVDADINGLR(Q)
1157.60451157.5915112502600(R) (Q)
1307.68791307.67827.42402491(R) (Q)
1791.79031791.7283354905120(R) (G)
1837.98161837.96608.53743890(R) (K)
1851.96921851.9275233213351(K) (N)
2171.07972171.0252252712880(K) (K)
2299.21252299.1202402712891(K) (N)
2510.20872510.1323304494710(K) (I)
2705.23442705.16172763940(R) (G)
2902.48882902.4111271992231(K) (T)
3264.64503264.5144403393670(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 37% (234/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/80 matches (16%).
Acc. #: Species: MOUSE Name: Annexin V (Lipocortin V) (Endonexin II) (Calphobindin I) (CBP-I) (Placental anticoagulant protein I) (PAP-I) (PP4) (Thromboplastin inhibitor) (Vascular anticoagulant-alpha) (VAC-alpha) (Anchorin CII)
Index: MW: 35753 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
698.3671698.3949-402002050(R) (R)
954.5282954.5413-141921990(K) (S)
1001.58471001.5995-151071150(K) (T)
1014.50211014.5148-1288950(R) (H)
1106.58121106.5846-3.12752830(R) (K)
1155.57721155.57581.22592690(K) (V)
1172.63601172.6825-401Met-ox 1501590(R)MLVVLLQANR(D)
1234.66751234.6796-9.82752841(R) (E)
1290.64631290.64044.61Met-ox 2892990(K)NFATSLYSMIK(G)
1613.89291613.9114-112262400(R) (S)
1733.93781733.8824322442580(R) (G)
1749.91691749.8773231Met-ox 2442580(R)SIPAYLAETLYYAMK(G)
1799.93271799.8968201851991(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (110/319AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Tissue-type plasminogen activator precursor (tPA) (t-PA) (t-plasminogen activator) (Alteplase) (Reteplase)
Index: MW: 62918 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
749.3291749.32525.285900(K) (C)
1014.50211014.5108-8.53783860(R) (F)
1179.60361179.6162-115495570(K) (D)
1277.72231277.664245pyroGlu 3033121(R)QYSQPQFRIK(G)
1699.85441699.8087271711841(R) (D)
1733.93781733.9083175255400(R) (D)
1749.91691749.90327.81Met-ox 5255400(R)MTLVGIISWGLGCGQK(D)
2184.16072184.15004.946620(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (81/562AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
775.3731775.3950-286926970(R) (S)
1179.60361179.5581396776850(K) (F)
1362.60221362.6186-125315410(R) (R)
1699.85441699.9343-4767811(K) (K)
1716.89181716.8267382883011(K) (L)
1791.79031791.8601-396776901(K) (R)
1851.96921851.9751-3.24214390(K) (G)
1994.01301994.0500-1925410(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (96/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Index: MW: 59621 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
745.3871745.4109-323193231(K) (L)
1065.50341065.5217-173914000(R) (V)
1158.58381158.5907-6.04674760(K) (N)
1232.58911232.6098-172973070(K) (Y)
1307.68791307.63843821310(K) (N)
1851.96921851.90233638541(K) (M)
2384.00902384.1114-432612811(K) (A)
2888.31862888.4551-471822061(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (110/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/80 matches (7%).
Acc. #: Species: MOUSE Name: THYMIDINE KINASE, CYTOSOLIC
Index: MW: 25760 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
744.3651744.3755-141871910(R) (K)
1065.50341065.4813211932030(K) (N)
1638.89551638.9001-2.82042180(K) (K)
1733.93781733.9148131160(-) (T)
1749.91691749.90974.11Met-ox 1160(-)MSYINLPTVLPSSPSK(T)
1994.01301993.9186471Met-ox 1491641(K)LTAVCMECFREAAYTK(R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (63/233AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 7/80 matches (8%).
Acc. #: Species: MOUSE Name: Zinc finger protein 147 (Tripartite motif protein 25) (Estrogen responsive finger protein) (Efp)
Index: MW: 71773 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1143.63751143.6010324314420(K) (A)
1232.58911232.6487-482492600(K) (R)
1383.69601383.6796123013121(K) (L)
1708.80281708.7542285505620(R) (I)
1791.79031791.7617165265400(K) (Q)
1803.91371803.8562325005130(R) (N)
2171.07972171.000037pyroGlu 1Met-ox 2422601(R)QEYMEMKAVIDAAETSSLR(R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (85/634AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 10/80 matches (12%).
Acc. #: Species: MOUSE Name: Estradiol 17 beta-dehydrogenase 4 (17-beta-HSD 4) (17-beta-hydroxysteroid dehydrogenase 4)
Index: MW: 79525 Da pI: 8.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
842.5021842.5212-2358641(K) (K)
1060.56231060.55407.9AcetN 1101(-)ASPLRFDGR(V)
1158.58381158.5577221Met-ox 2612700(R)NQPMTPEAVR(D)
1179.60361179.5646336696800(K) (G)
1265.64181265.6642-187027121(K) (L)
1302.65171302.7018-3851631(K) (R)
1613.89291613.9049-7.41691830(K) (K)
1765.81571765.8986-473123300(R) (L)
1803.91371803.9142-0.295425561(R) (A)
2902.48882902.4582114244501(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (133/735AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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