MS-Fit Search Results

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D73
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 2885 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/81(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	1.093e+014	26 (32)	42.0	32.1	25.1	57.9	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
2	4.038e+008	16 (19)	31.0	19.8	31.9	41.4	61988/5.1	HUMAN	Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
3	1.947e+005	9 (11)	23.0	11.1	9.09	115	59621/5.4	HUMAN	T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
4	6.832e+004	13 (16)	28.0	16.0	13.4	108	67255/9.1	MOUSE	Zinc finger protein 37 (Zfp-37) (Male germ cell specific zinc finger protein)
5	6.333e+004	15 (18)	21.0	18.5	22.6	106	96821/5.5	HUMAN	Tuftelin-interacting protein 11 (HSPC006)

46726	.	x	.	x	.	.	.	.	x	.	.	.	x	.	.	.	.	.	x	.	.	x	.	.	x	.	.	.	.	.	.	x	x	.	x	x	.	.	x	.	.	x	.	.	.	x	x	.	.	x	x	.	.	.	.	x	x	.	x	x	.	.	.	x	.	.	.	.	.	.	x	x	.	.	x	.	x	.	.	.	.
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27912	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	x	x	x	x	x	x	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.

Detailed Results

1. 26/81 matches (32%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
973.5742	973.5318	44		396	403	0	(K) (V)
1033.5569	1033.5166	39		484	492	0	(R) (M)
1066.5423	1066.5169	24		270	277	1	(K) (T)
1092.5776	1092.5034	68		603	616	0	(R) (G)
1141.5880	1141.5199	60		464	472	0	(R) (L)
1157.6293	1157.5149	99	1Met-ox	464	472	0	(R)DYQELMNTK(L)
1179.6422	1179.6010	35		377	386	0	(K) (H)
1265.6927	1265.6378	43		278	288	0	(R) (K)
1277.7520	1277.6337	93		247	257	1	(K) (N)
1277.7520	1277.7105	32		473	483	0	(K) (T)
1300.5779	1300.5302	37		258	267	0	(K) (N)
1302.6760	1302.7018	-20		393	403	1	(R) (V)
1302.6760	1302.7157	-30		344	355	0	(R) (A)
1316.5762	1316.5251	39	1Met-ox	258	267	0	(K)NMQDMVEDYR(N)
1340.6654	1340.6698	-3.3		365	376	1	(K) (Y)
1383.7225	1383.6909	23		186	197	0	(K) (V)
1393.7534	1393.7327	15		278	289	1	(R) (D)
1469.8546	1469.8440	7.2		396	407	1	(K) (L)
1475.7907	1475.7494	28		212	223	0	(K) (T)
1475.7907	1475.7858	3.3		200	211	0	(R) (W)
1523.8217	1523.7892	21		461	472	1	(R) (L)
1638.8898	1638.8604	18		186	199	1	(K) (F)
1657.8121	1657.7934	11		13	29	0	(R) (R)
1699.8437	1699.8251	11	pyroGlu	418	432	0	(K)QISNLQQSISDAEQR(G)
1716.8740	1716.8517	13		418	432	0	(K) (G)
1942.0133	1941.9882	13		444	460	1	(K) (L)
1993.9872	1993.9772	5.0		224	239	0	(R) (R)
2150.0778	2150.0783	-0.24		224	240	1	(R) (R)
2383.9593	2383.9524	2.9		519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (271/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/81 matches (19%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1060.6066 1060.5639 40 224 232 0 (K) (M)
1065.5439 1065.4999 41 154 162 0 (K) (L)
1066.5423 1066.4992 40 242 249 0 (K) (Q)
1082.5787 1082.4941 78 1Met-ox 242 249 0 (K)FEMEQNLR(Q)
1095.5968 1095.5475 45 184 191 1 (K) (G)
1140.5997 1140.5649 31 pyroGlu 250 260 0 (R)QGVDADINGLR(Q)
1157.6293 1157.5915 33 250 260 0 (R) (Q)
1235.6048 1235.5293 61 46 58 0 (R) (V)
1307.7208 1307.6782 33 240 249 1 (R) (Q)
1323.6997 1323.6731 20 1Met-ox 240 249 1 (R)IKFEMEQNLR(Q)
1791.7706 1791.7283 24 490 512 0 (R) (G)
1837.9998 1837.9660 18 374 389 0 (R) (K)
1851.9428 1851.9275 8.3 321 335 1 (K) (N)
2510.1674 2510.1323 14 449 471 0 (K) (I)
2705.1345 2705.1617 -10 63 94 0 (R) (G)
3264.6254 3264.5144 34 339 367 0 (K) (E)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1060.6066	1060.5639	40		224	232	0	(K) (M)
1065.5439	1065.4999	41		154	162	0	(K) (L)
1066.5423	1066.4992	40		242	249	0	(K) (Q)
1082.5787	1082.4941	78	1Met-ox	242	249	0	(K)FEMEQNLR(Q)
1095.5968	1095.5475	45		184	191	1	(K) (G)
1140.5997	1140.5649	31	pyroGlu	250	260	0	(R)QGVDADINGLR(Q)
1157.6293	1157.5915	33		250	260	0	(R) (Q)
1235.6048	1235.5293	61		46	58	0	(R) (V)
1307.7208	1307.6782	33		240	249	1	(R) (Q)
1323.6997	1323.6731	20	1Met-ox	240	249	1	(R)IKFEMEQNLR(Q)
1791.7706	1791.7283	24		490	512	0	(R) (G)
1837.9998	1837.9660	18		374	389	0	(R) (K)
1851.9428	1851.9275	8.3		321	335	1	(K) (N)
2510.1674	2510.1323	14		449	471	0	(K) (I)
2705.1345	2705.1617	-10		63	94	0	(R) (G)
3264.6254	3264.5144	34		339	367	0	(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (198/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/81 matches (11%).
Acc. #: Species: HUMAN Name: T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Index: MW: 59621 Da pI: 5.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1065.5439 1065.5217 21 391 400 0 (R) (V)
1158.6297 1158.5907 34 467 476 0 (K) (N)
1232.6403 1232.6098 25 297 307 0 (K) (Y)
1307.7208 1307.6384 63 21 31 0 (K) (N)
1365.7268 1365.5956 96 379 390 0 (R) (A)
1851.9428 1851.9023 22 38 54 1 (K) (M)
1890.9739 1891.0475 -39 521 539 0 (R) (K)
2022.9050 2023.0581 -76 139 155 1 (K) (D)
2383.9593 2384.1114 -64 261 281 1 (K) (A)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
1065.5439	1065.5217	21	391	400	0	(R) (V)
1158.6297	1158.5907	34	467	476	0	(K) (N)
1232.6403	1232.6098	25	297	307	0	(K) (Y)
1307.7208	1307.6384	63	21	31	0	(K) (N)
1365.7268	1365.5956	96	379	390	0	(R) (A)
1851.9428	1851.9023	22	38	54	1	(K) (M)
1890.9739	1891.0475	-39	521	539	0	(R) (K)
2022.9050	2023.0581	-76	139	155	1	(K) (D)
2383.9593	2384.1114	-64	261	281	1	(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (128/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/81 matches (16%).
Acc. #: Species: MOUSE Name: Zinc finger protein 37 (Zfp-37) (Male germ cell specific zinc finger protein)
Index: MW: 67255 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1139.6143 1139.5445 61 219 228 1 (R) (I)
1139.6143 1139.5584 49 121 130 0 (K) (S)
1140.5997 1140.5836 14 pyroGlu 574 583 1 (K)QNASLTKHMK(I)
1157.6293 1157.6101 17 574 583 1 (K) (I)
1179.6422 1179.5945 40 406 415 1 (K) (I)
1291.5722 1291.6242 -40 150 161 1 (R) (K)
1340.6654 1340.6745 -6.8 237 247 1 (K) (A)
1493.7819 1493.6960 58 459 471 0 (K) (T)
1791.7706 1791.7642 3.6 1Met-ox 33 48 0 (K)LENCSNPASMGNQDPK(Q)
1837.9998 1837.8213 97 360 374 1 (R) (S)
1851.9428 1851.8006 77 304 318 0 (R) (A)
2015.9774 2016.0779 -50 324 340 1 (K) (C)
2383.9593 2384.0651 -44 276 295 0 (R) (S)
3314.2430 3314.5323 -87 pyroGlu 268 295 1 (K)QGLLDHQRTHTGEKPYECNECGIAFSQK(S)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1139.6143	1139.5445	61		219	228	1	(R) (I)
1139.6143	1139.5584	49		121	130	0	(K) (S)
1140.5997	1140.5836	14	pyroGlu	574	583	1	(K)QNASLTKHMK(I)
1157.6293	1157.6101	17		574	583	1	(K) (I)
1179.6422	1179.5945	40		406	415	1	(K) (I)
1291.5722	1291.6242	-40		150	161	1	(R) (K)
1340.6654	1340.6745	-6.8		237	247	1	(K) (A)
1493.7819	1493.6960	58		459	471	0	(K) (T)
1791.7706	1791.7642	3.6	1Met-ox	33	48	0	(K)LENCSNPASMGNQDPK(Q)
1837.9998	1837.8213	97		360	374	1	(R) (S)
1851.9428	1851.8006	77		304	318	0	(R) (A)
2015.9774	2016.0779	-50		324	340	1	(K) (C)
2383.9593	2384.0651	-44		276	295	0	(R) (S)
3314.2430	3314.5323	-87	pyroGlu	268	295	1	(K)QGLLDHQRTHTGEKPYECNECGIAFSQK(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (167/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 15/81 matches (18%).
Acc. #: Species: HUMAN Name: Tuftelin-interacting protein 11 (HSPC006)
Index: MW: 96821 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1141.5880 1141.6118 -21 491 499 0 (R) (N)
1151.6890 1151.5809 94 186 197 1 (K) (T)
1259.7055 1259.6523 42 238 247 1 (K) (A)
1277.7520 1277.7040 38 1Met-ox 156 166 1 (K)LLQKMGYVPGR(G)
1300.5779 1300.6095 -24 444 454 0 (K) (W)
1302.6760 1302.7422 -51 563 573 1 (R) (L)
1307.7208 1307.5975 94 376 385 0 (K) (R)
1310.7232 1310.6017 93 47 57 0 (K) (D)
1316.5762 1316.5581 14 742 751 0 (K) (R)
1323.6997 1323.5925 81 1Met-ox 376 385 0 (K)VLEMVEECER(R)
1393.7534 1393.7150 28 1Met-ox 761 774 0 (R)GIGVAASSVPMNFK(D)
1475.7907 1475.7873 2.3 480 490 0 (R) (N)
1475.7907 1475.7892 1.0 262 274 1 (K) (E)
1523.8217 1523.7277 62 1Met-ox 714 728 0 (R)AVSSNVGAYMQPGAR(E)
1708.7723 1708.9022 -76 71 86 1 (R) (K)
2015.9774 2016.0072 -15 1Met-ox 359 375 1 (K)MTEVLDHEERVISNLSK(V)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1141.5880	1141.6118	-21		491	499	0	(R) (N)
1151.6890	1151.5809	94		186	197	1	(K) (T)
1259.7055	1259.6523	42		238	247	1	(K) (A)
1277.7520	1277.7040	38	1Met-ox	156	166	1	(K)LLQKMGYVPGR(G)
1300.5779	1300.6095	-24		444	454	0	(K) (W)
1302.6760	1302.7422	-51		563	573	1	(R) (L)
1307.7208	1307.5975	94		376	385	0	(K) (R)
1310.7232	1310.6017	93		47	57	0	(K) (D)
1316.5762	1316.5581	14		742	751	0	(K) (R)
1323.6997	1323.5925	81	1Met-ox	376	385	0	(K)VLEMVEECER(R)
1393.7534	1393.7150	28	1Met-ox	761	774	0	(R)GIGVAASSVPMNFK(D)
1475.7907	1475.7873	2.3		480	490	0	(R) (N)
1475.7907	1475.7892	1.0		262	274	1	(K) (E)
1523.8217	1523.7277	62	1Met-ox	714	728	0	(R)AVSSNVGAYMQPGAR(E)
1708.7723	1708.9022	-76		71	86	1	(R) (K)
2015.9774	2016.0072	-15	1Met-ox	359	375	1	(K)MTEVLDHEERVISNLSK(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (181/837AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):