MS-Fit Search Results

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D74
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 3921 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/118(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	8.189e+007	15 (12)	48.0	12.7	62.8	26.2	34482/5.0	HUMAN	Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)
2	4.261e+007	15 (12)	27.0	12.7	63.5	42.6	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
3	6.243e+006	12 (10)	39.0	10.2	56.3	41.0	35937/4.9	HUMAN	Annexin V (Lipocortin V) (Endonexin II) (Calphobindin I) (CBP-I) (Placental anticoagulant protein I) (PAP-I) (PP4) (Thromboplastin inhibitor) (Vascular anticoagulant-alpha) (VAC-alpha) (Anchorin CII)
4	3.010e+006	14 (11)	17.0	11.9	46.4	67.8	88830/5.9	HUMAN	Centromere/kinetochore protein zw10 homolog
5	7.993e+005	12 (10)	25.0	10.2	48.8	85.4	69501/8.2	HUMAN	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>

83720	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	x	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	x	x	x	.	.	.	.	.	.
46726	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	x	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.
29851	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x
19475	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	x	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.
83345	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 15/118 matches (12%).
Acc. #: Species: HUMAN Name: Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)
Index: MW: 34482 Da pI: 5.0

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
971.6128	971.5638	50		159	166	1	(K) (K)
1017.5769	1017.5217	54		98	106	0	(R) (E)
1311.7420	1311.6697	55		49	58	1	(R) (A)
1339.7058	1339.6105	71		86	96	0	(R) (R)
1355.6841	1355.6054	58	1Met-ox	86	96	0	(R)MFADYLAHESR(R)
1495.8171	1495.7116	71		86	97	1	(R) (D)
1511.8264	1511.7065	79	1Met-ox	86	97	1	(R)MFADYLAHESRR(D)
1536.8481	1536.7328	75	1Met-ox	98	110	1	(R)DSIVAELDREMSR(S)
1737.9188	1737.8448	43		294	308	1	(K) (-)
1750.9302	1750.7998	74	1Met-ox	198	211	0	(K)LQDAYYIFQEMADK(C)
2076.0871	2075.9609	61		24	40	0	(K) (V)
2155.2851	2155.1763	50		66	85	0	(K) (M)
2242.2908	2242.0895	90	1Met-ox	212	231	0	(K)CSPTLLLLNGQAACHMAQGR(W)
2242.2908	2242.1575	59		139	158	0	(R) (L)
2258.3045	2258.1525	67	1Met-ox	139	158	0	(R)ALHQGDSLECTAMTVQILLK(L)
2283.3778	2283.2713	47		65	85	1	(R) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 48% (150/308AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 15/118 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
973.6045 973.5318 75 396 403 0 (K) (V)
1014.5796 1014.4791 99 1Met-ox 1 8 1 (-)MSRQFSSR(S)
1033.6054 1033.5166 86 484 492 0 (R) (M)
1179.6885 1179.6010 74 377 386 0 (K) (H)
1231.7102 1231.6283 67 356 366 1 (K) (A)
1265.6772 1265.6378 31 278 288 0 (R) (K)
1277.8073 1277.7105 76 473 483 0 (K) (T)
1340.7076 1340.6698 28 365 376 1 (K) (Y)
1383.7561 1383.6909 47 186 197 0 (K) (V)
1475.8665 1475.7494 79 212 223 0 (K) (T)
1475.8665 1475.7858 55 200 211 0 (R) (W)
1638.9438 1638.8604 51 186 199 1 (K) (F)
1688.0219 1687.9707 30 66 82 1 (R) (G)
1716.9552 1716.8517 60 418 432 0 (K) (G)
2286.3071 2286.1254 79 367 386 1 (K) (H)
2384.1402 2383.9524 79 519 549 0 (R) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
973.6045	973.5318	75		396	403	0	(K) (V)
1014.5796	1014.4791	99	1Met-ox	1	8	1	(-)MSRQFSSR(S)
1033.6054	1033.5166	86		484	492	0	(R) (M)
1179.6885	1179.6010	74		377	386	0	(K) (H)
1231.7102	1231.6283	67		356	366	1	(K) (A)
1265.6772	1265.6378	31		278	288	0	(R) (K)
1277.8073	1277.7105	76		473	483	0	(K) (T)
1340.7076	1340.6698	28		365	376	1	(K) (Y)
1383.7561	1383.6909	47		186	197	0	(K) (V)
1475.8665	1475.7494	79		212	223	0	(K) (T)
1475.8665	1475.7858	55		200	211	0	(R) (W)
1638.9438	1638.8604	51		186	199	1	(K) (F)
1688.0219	1687.9707	30		66	82	1	(R) (G)
1716.9552	1716.8517	60		418	432	0	(K) (G)
2286.3071	2286.1254	79		367	386	1	(K) (H)
2384.1402	2383.9524	79		519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (179/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/118 matches (10%).
Acc. #: Species: HUMAN Name: Annexin V (Lipocortin V) (Endonexin II) (Calphobindin I) (CBP-I) (Placental anticoagulant protein I) (PAP-I) (PP4) (Thromboplastin inhibitor) (Vascular anticoagulant-alpha) (VAC-alpha) (Anchorin CII)
Index: MW: 35937 Da pI: 4.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
954.6056 954.5413 67 194 201 0 (K) (S)
1001.6365 1001.5995 37 109 117 0 (K) (T)
1014.5796 1014.5148 64 90 97 0 (R) (H)
1106.6546 1106.5846 63 277 285 0 (R) (K)
1234.7574 1234.6796 63 277 286 1 (R) (E)
1290.7474 1290.6404 83 1Met-ox 291 301 0 (K)NFATSLYSMIK(G)
1340.7076 1340.6123 71 7 18 0 (R) (A)
1463.8167 1463.7898 18 90 101 1 (R) (G)
1613.9470 1613.9114 22 228 242 0 (R) (S)
1705.0144 1704.9020 66 30 45 0 (K) (S)
2097.1421 2096.9889 73 7 25 1 (R) (K)
2888.3784 2888.2386 48 127 151 0 (K) (M)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
954.6056	954.5413	67		194	201	0	(K) (S)
1001.6365	1001.5995	37		109	117	0	(K) (T)
1014.5796	1014.5148	64		90	97	0	(R) (H)
1106.6546	1106.5846	63		277	285	0	(R) (K)
1234.7574	1234.6796	63		277	286	1	(R) (E)
1290.7474	1290.6404	83	1Met-ox	291	301	0	(K)NFATSLYSMIK(G)
1340.7076	1340.6123	71		7	18	0	(R) (A)
1463.8167	1463.7898	18		90	101	1	(R) (G)
1613.9470	1613.9114	22		228	242	0	(R) (S)
1705.0144	1704.9020	66		30	45	0	(K) (S)
2097.1421	2096.9889	73		7	25	1	(R) (K)
2888.3784	2888.2386	48		127	151	0	(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (125/320AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 14/118 matches (11%).
Acc. #: Species: HUMAN Name: Centromere/kinetochore protein zw10 homolog
Index: MW: 88830 Da pI: 5.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
973.6045 973.5934 11 102 110 0 (R) (Q)
1043.5621 1043.5849 -22 595 603 1 (R) (L)
1045.6274 1045.5464 77 404 412 0 (K) (N)
1045.6274 1045.5781 47 66 74 0 (K) (S)
1165.6781 1165.6138 55 1Met-ox 161 170 0 (K)SLSMELTIQK(Q)
1179.6885 1179.5866 86 36 45 1 (K) (Y)
1339.7058 1339.6503 41 584 594 0 (R) (A)
1355.6841 1355.6452 29 1Met-ox 584 594 0 (R)LGTECFLAQMR(A)
1434.8358 1434.7242 78 391 402 1 (K) (K)
1495.8171 1495.7514 44 583 594 1 (R) (A)
1511.8264 1511.7463 53 1Met-ox 583 594 1 (R)RLGTECFLAQMR(A)
1716.9552 1716.9648 -5.6 746 762 1 (K) (A)
1963.9890 1963.9323 29 688 705 0 (K) (N)
2242.2908 2242.1066 82 46 65 0 (K) (L)
2258.3045 2258.1015 90 1Met-ox 46 65 0 (K)YSEFLPSMQSAQGLITQVDK(L)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
973.6045	973.5934	11		102	110	0	(R) (Q)
1043.5621	1043.5849	-22		595	603	1	(R) (L)
1045.6274	1045.5464	77		404	412	0	(K) (N)
1045.6274	1045.5781	47		66	74	0	(K) (S)
1165.6781	1165.6138	55	1Met-ox	161	170	0	(K)SLSMELTIQK(Q)
1179.6885	1179.5866	86		36	45	1	(K) (Y)
1339.7058	1339.6503	41		584	594	0	(R) (A)
1355.6841	1355.6452	29	1Met-ox	584	594	0	(R)LGTECFLAQMR(A)
1434.8358	1434.7242	78		391	402	1	(K) (K)
1495.8171	1495.7514	44		583	594	1	(R) (A)
1511.8264	1511.7463	53	1Met-ox	583	594	1	(R)RLGTECFLAQMR(A)
1716.9552	1716.9648	-5.6		746	762	1	(K) (A)
1963.9890	1963.9323	29		688	705	0	(K) (N)
2242.2908	2242.1066	82		46	65	0	(K) (L)
2258.3045	2258.1015	90	1Met-ox	46	65	0	(K)YSEFLPSMQSAQGLITQVDK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (135/779AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 12/118 matches (10%).
Acc. #: Species: HUMAN Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>
Index: MW: 69501 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1085.4730 1085.4726 0.36 561 569 0 (K) (H)
1133.5768 1133.6180 -36 31 41 1 (R) (G)
1307.7514 1307.6418 84 513 524 1 (R) (D)
1311.7420 1311.6884 41 501 512 0 (R) (D)
1340.7076 1340.6850 17 223 233 0 (K) (L)
1383.7561 1383.6513 76 342 352 1 (R) (H)
1497.8535 1497.7562 65 15 27 0 (K) (N)
1511.8264 1511.7317 63 399 411 0 (K) (N)
1718.9769 1718.8397 80 1Met-ox 412 426 0 (R)NPVHNGHALLMQDTR(R)
1773.9999 1773.8270 97 587 602 1 (R) (V)
1941.0668 1940.8878 92 174 190 0 (K) (V)
2083.1369 2083.1228 6.7 252 269 0 (K) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1085.4730	1085.4726	0.36		561	569	0	(K) (H)
1133.5768	1133.6180	-36		31	41	1	(R) (G)
1307.7514	1307.6418	84		513	524	1	(R) (D)
1311.7420	1311.6884	41		501	512	0	(R) (D)
1340.7076	1340.6850	17		223	233	0	(K) (L)
1383.7561	1383.6513	76		342	352	1	(R) (H)
1497.8535	1497.7562	65		15	27	0	(K) (N)
1511.8264	1511.7317	63		399	411	0	(K) (N)
1718.9769	1718.8397	80	1Met-ox	412	426	0	(R)NPVHNGHALLMQDTR(R)
1773.9999	1773.8270	97		587	602	1	(R) (V)
1941.0668	1940.8878	92		174	190	0	(K) (V)
2083.1369	2083.1228	6.7		252	269	0	(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (158/614AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):