MS-Fit Search Results


.

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D75
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1287 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/86(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.998e+00816 (18)61.018.6-7.0519.6 29999/5.0HUMANMicrotubule-associated protein RP/EB family member 1 (APC-binding protein EB1)
21.609e+00814 (16)30.016.3-10.736.0 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
37.129e+00410 (11)18.011.6-21.341.6 80625/7.3HUMANProtein-glutamine gamma-glutamyltransferase 3-like (Transglutaminase 3-like) (TGase 3-like)
42.137e+0048 (9)31.09.3-11.726.7 29885/9.8MOUSE40S ribosomal protein S3A
52.133e+0048 (9)31.09.3-11.726.7 29945/9.8HUMAN40S ribosomal protein S3a
69748x......................x...x..x.......x......x............x.....x.x..x..x.xx.....xx.x.
46726.............x.....................x.xx.xxx......xx..x.....xx.x....................x..
7360.x.....................x...........x.......xx..........x...x...x..............x...x...
67475.x.x.................................x...x.....................x...x...x.....x........
24207.x.x.................................x...x.....................x...x...x.....x........


Detailed Results

1. 16/86 matches (18%).
Acc. #: Species: HUMAN Name: Microtubule-associated protein RP/EB family member 1 (APC-binding protein EB1)
Index: MW: 29999 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
932.5574932.5682-1277841(K) (M)
1045.53651045.5471-102152221(R) (N)
1061.50691061.50561.22132201(K) (L)
1076.49341076.4689231141220(K) (D)
1231.64321231.6534-8.32052141(K) (D)
1320.64461320.6588-1167760(K) (I)
1634.75361634.7644-6.61011120(K) (K)
1754.83821754.8561-10AcetN 1170(-)AVNVYSTSVTSDNLSR(H)
1819.86241819.8808-10991121(K) (K)
1925.03601925.0568-111511680(K) (T)
1963.86101963.8826-111141301(K) (Q)
2002.06052002.0973-18pyroGlu 1311500(R)QGQETAVAPSLVAPALNKPK(K)
2019.10352019.1239-101311500(R) (K)
2284.06472284.0879-102232410(R) (I)
2298.06942298.1036-151832040(K) (L)
2442.20852442.19356.21Met-ox 1822041(R)KNPGVGNGDDEAAELMQQVNVLK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 61% (164/268AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/86 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
999.4547999.44579.02902980(K) (V)
999.4547999.4972-433873951(R) (I)
1179.58481179.6010-143773860(K) (H)
1217.59391217.6166-196346440(R) (-)
1231.64321231.6283123563661(K) (A)
1265.63541265.6378-1.92782880(R) (K)
1277.71611277.71054.44734830(K) (T)
1300.54161300.53028.82582670(K) (N)
1357.70141357.69633.74444550(K) (E)
1383.66081383.6909-221861970(K) (V)
1475.74051475.7494-6.02122230(K) (T)
1475.74051475.7858-312002110(R) (W)
1638.81491638.8604-281861991(K) (F)
1687.89991687.9707-4266821(R) (G)
1716.84251716.8517-5.34184320(K) (G)
2383.95512383.95241.15195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (195/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/86 matches (11%).
Acc. #: Species: HUMAN Name: Protein-glutamine gamma-glutamyltransferase 3-like (Transglutaminase 3-like) (TGase 3-like)
Index: MW: 80625 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
935.4622935.4951-354744820(R) (R)
1045.53651045.5464-9.53823910(R) (E)
1179.58481179.6010-1485940(K) (G)
1307.66301307.7099-366146240(K) (V)
1319.67291319.6378272332440(R) (G)
1493.73851493.8076-4647591(R) (A)
1638.81491638.8339-124414551(K) (Y)
1718.86951718.9117-25pyroGlu 6947080(R)QLQVDLVSPHFPDIK(G)
2091.94782092.0034-2729460(R) (R)
2298.06942298.1532-362452651(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (134/719AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/86 matches (9%).
Acc. #: Species: MOUSE Name: 40S ribosomal protein S3A
Index: MW: 29885 Da pI: 9.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
935.4622935.4773-161Met-ox 35420(K)APAMFNIR(N)
938.4682938.4947-281291360(K) (L)
1217.59391217.6027-7.21531620(K) (Q)
1277.71611277.71054.484941(K) (N)
1718.86951718.86880.382002130(K) (K)
1838.88531838.88152.11681821(K) (E)
1951.92531951.9765-2666820(R) (K)
2080.02382080.0715-2366831(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (84/264AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/86 matches (9%).
Acc. #: Species: HUMAN Name: 40S ribosomal protein S3a
Index: MW: 29945 Da pI: 9.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
935.4622935.4773-161Met-ox 35420(K)APAMFNIR(N)
938.4682938.4947-281291360(K) (L)
1217.59391217.6027-7.21531620(K) (Q)
1277.71611277.71054.484941(K) (N)
1718.86951718.86880.382002130(K) (K)
1838.88531838.88152.11681821(K) (E)
1951.92531951.9765-2666820(R) (K)
2080.02382080.0715-2366831(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (84/264AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.