MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D76
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 873 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/49(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.659e+00814 (28)27.028.653.927.9 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
22.397e+00813 (26)58.026.547.240.8 26923/5.1HUMANChloride intracellular channel protein 1 (Nuclear chloride ion channel 27) (NCC27) (p64 CLCP) (Chloride channel ABP)
31.807e+00510 (20)23.020.456.746.9 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
41.239e+00510 (20)30.020.440.535.8 27013/5.1MOUSEChloride intracellular channel protein 1 (Nuclear chloride ion channel 27) (NCC27) (p64 CLCP)
51.322e+0047 (14)12.014.341.6103 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
628505 (10)14.010.24.29100.0 61865/8.9HUMANProbable glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial precursor (Glu-ADT subunit B) (Cytochrome oxidase assembly factor PET112 homolog)
728466 (12)20.012.20.660116 43136/6.3HUMANMitogen-activated protein kinase 3 (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase 1) (MAPK 1) (p44-ERK1) (ERT2) (p44-MAPK) (Microtubule-associated protein-2 kinase)
828028 (16)9.016.321.889.8 94506/5.6HUMANOsmotic stress protein 94 (Heat shock 70-related protein APG-1)
918277 (14)35.014.311.7110 26887/10.9HUMANINT-2 proto-oncogene protein precursor (Fibroblast growth factor-3) (FGF-3) (HBGF-3)
1018048 (16)14.016.321.9105 80789/9.4HUMANMetastasis-associated protein MTA1
45313..x...x...x...........x..xxxx..x.x..x.x..x.x.....
110894x....x.....x...x...x..x.......x.x.....x.x.x..x..x
97443.x..x.............x..x.x.....x.......x.x....x.x..
69447x....x.....x...x...x..x.......x.x.......x.x......
42717.............x......x.....x.........x..x.x...x...
110426.................x..x................x.....x.x...
87184.....x.........x.x..........x......x...........x.
66994.x..x..........x......x.x....x...x...........x...
71588....x..............x.......x.xx.....x..........x.
51371.x..........x.........x.x.x.x.................x.x


Detailed Results

1. 14/49 matches (28%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
832.5146832.48923075820(K) (G)
973.5570973.5318263964030(K) (V)
1033.56451033.5166464844920(R) (M)
1092.57861092.5034696036160(R) (G)
1141.59321141.5199644644720(R) (L)
1179.66201179.6010523773860(K) (H)
1277.77681277.7105524734830(K) (T)
1300.60591300.5302582582670(K) (N)
1383.76051383.6909501861970(K) (V)
1475.84361475.7494642122230(K) (T)
1475.84361475.7858392002110(R) (W)
1716.95441716.8517604184320(K) (G)
1845.08751844.9466764174321(K) (G)
1994.09371993.9772582242390(R) (R)
2384.10282383.9524635195490(R) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 27% (178/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/49 matches (26%).
Acc. #: Species: HUMAN Name: Chloride intracellular channel protein 1 (Nuclear chloride ion channel 27) (NCC27) (p64 CLCP) (Chloride channel ABP)
Index: MW: 26923 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
728.4045728.39838.51141190(K) (N)
957.5161957.4794382092160(R) (E)
1037.60041037.5420561962040(R) (G)
1049.65531049.62223230370(R) (G)
1065.62961065.6171121Met-ox 30370(R)LFMVLWLK(G)
1092.57861092.52604821290(K) (L)
1356.77371356.7064501942041(K) (G)
1458.82511458.748053AcetN 1130(-)AEEQPQVELFVK(A)
1845.08751844.975861961130(K) (F)
1947.10391946.9897591671830(K) (L)
2075.18812075.0847501661831(R) (L)
2587.34652587.1398802172380(R) (A)
2992.59372992.3911681391650(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 58% (140/241AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/49 matches (20%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
745.4313745.4208143683730(K) (H)
897.4429897.4140322332390(R) (I)
1060.59991060.5639342242320(K) (M)
1081.60091081.4948981Met-ox 1541620(K)STMQELNSR(L)
1081.60091081.51398034451(R) (F)
1095.60971095.5475571841911(K) (G)
1307.75041307.6782552402491(R) (Q)
1838.07971837.9660623743890(R) (K)
1852.03231851.9275573213351(K) (N)
2510.29382510.1323644494710(K) (I)
2705.35242705.16177163940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (147/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/49 matches (20%).
Acc. #: Species: MOUSE Name: Chloride intracellular channel protein 1 (Nuclear chloride ion channel 27) (NCC27) (p64 CLCP)
Index: MW: 27013 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
728.4045728.39838.51141190(K) (N)
957.5161957.4794382092160(R) (E)
1037.60041037.5420561962040(R) (G)
1049.65531049.62223230370(R) (G)
1065.62961065.6171121Met-ox 30370(R)LFMVLWLK(G)
1092.57861092.52604821290(K) (L)
1356.77371356.7064501942041(K) (G)
1458.82511458.748053AcetN 1130(-)AEEQPQVELFVK(A)
1947.10391946.9897591671830(K) (L)
2075.18812075.0847501661831(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (73/241AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/49 matches (14%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1040.58251040.6468-622052130(R) (M)
1078.55801078.4852675735810(R) (A)
1179.66201179.5581886776850(K) (F)
1716.95441716.8267742883011(K) (L)
1852.03231851.9751314214390(K) (G)
1994.09371994.05002225410(R) (T)
2587.34652587.1638717237421(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (97/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 5/49 matches (10%).
Acc. #: Species: HUMAN Name: Probable glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial precursor (Glu-ADT subunit B) (Cytochrome oxidase assembly factor PET112 homolog)
Index: MW: 61865 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1059.56921059.5798-102042120(R) (A)
1078.55801078.55730.594704770(K) (R)
1838.07971837.95206941571(R) (K)
2384.10282384.2608-662382590(R) (V)
2587.34652587.2748282132370(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (81/557AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 6/49 matches (12%).
Acc. #: Species: HUMAN Name: Mitogen-activated protein kinase 3 (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase 1) (MAPK 1) (p44-ERK1) (ERT2) (p44-MAPK) (Microtubule-associated protein-2 kinase)
Index: MW: 43136 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
957.5161957.5046123713790(R) (-)
1049.65531049.5631882792870(R) (T)
1059.56921059.6355-632902981(K) (S)
1300.60591300.6068-0.721150(-) (R)
1707.88111707.82773172841(K) (T)
2717.24242717.4159-642252480(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (79/379AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 8/49 matches (16%).
Acc. #: Species: HUMAN Name: Osmotic stress protein 94 (Heat shock 70-related protein APG-1)
Index: MW: 94506 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
745.4313745.4460-207687740(K) (S)
897.4429897.4583-174234300(K) (V)
1049.65531049.5519996426500(K) (L)
1092.57861092.5876-8.26836911(K) (Y)
1108.57441108.5825-7.31Met-ox 6836911(K)KYGQPIQMK(Y)
1307.75041307.6959421861960(K) (N)
1475.84361475.7342743613740(K) (G)
2587.34652587.3150123233461(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 9% (82/839AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 7/49 matches (14%).
Acc. #: Species: HUMAN Name: INT-2 proto-oncogene protein precursor (Fibroblast growth factor-3) (FGF-3) (HBGF-3)
Index: MW: 26887 Da pI: 10.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
897.4429897.4868-4947531(R) (Y)
1065.62961065.5733531521600(R) (G)
1277.77681277.7330341932040(R) (G)
1307.75041307.7072331331441(R) (R)
1356.77371356.709847901011(R) (R)
1716.95441716.870949pyroGlu 1461601(R)QPSAERLWYVSVNGK(G)
2717.24242717.4725-8564890(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (84/239AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 8/49 matches (16%).
Acc. #: Species: HUMAN Name: Metastasis-associated protein MTA1
Index: MW: 80789 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
745.4313745.40962927320(R) (T)
1038.59371038.5406511Met-ox 4274351(K)YGGLKMPTR(L)
1092.57861092.55122562700(K) (A)
1108.57441108.5791-4.34694770(R) (L)
1179.66201179.6261301731820(R) (E)
1300.60591300.7265-934985090(R) (A)
2705.35242705.1929592833051(R) (D)
2992.59372992.3610782Met-ox 1251(-)MAANMYRVGDYVYFENSSSNPYLIR(R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (103/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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