MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D77
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 261 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/42(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.662e+00613 (30)47.031.068.033.2 28433/5.2HUMANProteasome subunit alpha type 3 (Proteasome component C8) (Macropain subunit C8) (Multicatalytic endopeptidase complex subunit C8)
22.302e+00512 (28)41.028.665.428.6 28405/5.3MOUSEProteasome subunit alpha type 3 (Proteasome component C8) (Macropain subunit C8) (Multicatalytic endopeptidase complex subunit C8) (Proteasome subunit K)
31.737e+0047 (16)15.016.778.028.4 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
420066 (14)9.014.339.2117 80509/5.5HUMANAdseverin (Scinderin)
59855 (11)29.011.940.955.5 31627/8.6MOUSESteroidogenic acute regulatory protein, mitochondrial precursor (StAR) (StARD1) (Luteinizing hormone-induced protein)
66797 (16)9.016.741.183.6 99647/5.4MOUSERod cGMP-specific 3',5'-cyclic phosphodiesterase alpha-subunit (GMP-PDE alpha)
74744 (9)13.09.559.667.6 44954/6.3MOUSEGPI-anchor transamidase (GPI transamidase) (Phosphatidylinositol-glycan biosynthesis, class K protein) (PIG-K)
83545 (11)9.011.930.5112 64461/8.2HUMANTNF receptor associated factor 3 (CD40 receptor associated factor 1) (CRAF1) (CD40 binding protein) (CD40BP) (LMP1 associated protein) (LAP1) (CAP-1)
92905 (11)9.011.959.430.2 57458/5.5MOUSEGlycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase) (GK)
102895 (11)11.011.935.666.6 75285/7.6HUMANChloride channel protein CLC-KA (ClC-K1)
11032.x.xxxx..xxx.....x.x.x......x...x.........
91891.x.xxxx..xxx.....x.x.x..........x.........
45313........xx..x.....x...x.x........x........
10776.x..........x..........x......x..x.x......
112207.............x............xx.........x...x
89288.x..xx.....x...xx..............x..........
63489.........................x...xx......x....
106355....xx...x......x......x..................
2240....xx...x........x...............x.......
96983....x...........x.............x..x..x.....


Detailed Results

1. 13/42 matches (30%).
Acc. #: Species: HUMAN Name: Proteasome subunit alpha type 3 (Proteasome component C8) (Macropain subunit C8) (Multicatalytic endopeptidase complex subunit C8)
Index: MW: 28433 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
989.5359989.4904462312381(K) (Y)
1095.62671095.54357658661(K) (L)
1114.61461114.56074821290(R) (A)
1130.60781130.5556461Met-ox 21290(R)VFQVEYAMK(A)
1152.67541152.6053611011100(R) (H)
1217.73351217.64906930410(K) (C)
1228.76331228.6941561972060(K) (D)
1251.71771251.64076242521(R) (L)
1380.84711380.74227673860(R) (S)
1396.84271396.7371761Met-ox 73860(R)HVGMAVAGLLADAR(S)
1471.93371471.8161801972081(K) (A)
1821.09871820.9183992092240(K) (H)
1960.06751959.893689AcetN 1200(-)SSIGTGYDLSASTFSPDGR(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 47% (121/255AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/42 matches (28%).
Acc. #: Species: MOUSE Name: Proteasome subunit alpha type 3 (Proteasome component C8) (Macropain subunit C8) (Multicatalytic endopeptidase complex subunit C8) (Proteasome subunit K)
Index: MW: 28405 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
989.5359989.4904462312381(K) (Y)
1095.62671095.54357658661(K) (L)
1114.61461114.56074821290(R) (A)
1130.60781130.5556461Met-ox 21290(R)VFQVEYAMK(A)
1152.67541152.6053611011100(R) (H)
1217.73351217.64906930410(K) (C)
1228.76331228.6941561972060(K) (D)
1251.71771251.64076242521(R) (L)
1380.84711380.74227673860(R) (S)
1396.84271396.7371761Met-ox 73860(R)HVGMAVAGLLADAR(S)
1471.93371471.8161801972081(K) (A)
1960.06751959.893689AcetN 1200(-)SSIGTGYDLSASTFSPDGR(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (105/255AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/42 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.68011179.6010673773860(K) (H)
1217.73351217.6166966346440(R) (-)
1277.78301277.7105574734830(K) (T)
1383.80501383.6909831861970(K) (V)
1475.87681475.7494862122230(K) (T)
1475.87681475.7858622002110(R) (W)
1688.11861687.97078866821(R) (G)
1994.14881993.9772862242390(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (101/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/42 matches (14%).
Acc. #: Species: HUMAN Name: Adseverin (Scinderin)
Index: MW: 80509 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
989.5359989.6035-684884950(K) (N)
1277.78301277.7469283593691(K) (Q)
1493.86761493.7276941501620(R) (G)
1838.10361837.9772691191370(K) (R)
1994.14881994.0783351191381(K) (L)
2097.15112096.9889775986150(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (70/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/42 matches (11%).
Acc. #: Species: MOUSE Name: Steroidogenic acute regulatory protein, mitochondrial precursor (StAR) (StARD1) (Luteinizing hormone-induced protein)
Index: MW: 31627 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1300.66431300.65715.51211310(R) (L)
1765.91061765.8770191151(-) (H)
1791.93131791.8410501Met-ox 1401541(R)MEAMGEWNPNVKEIK(V)
2225.33162225.2042572532710(K) (K)
2705.49222705.2962721922161(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (85/284AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 7/42 matches (16%).
Acc. #: Species: MOUSE Name: Rod cGMP-specific 3',5'-cyclic phosphodiesterase alpha-subunit (GMP-PDE alpha)
Index: MW: 99647 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
989.5359989.4957415565620(R) (H)
1114.61461114.6294-133843930(K) (K)
1130.60781130.6244-151Met-ox 3843930(K)NVLSMPIVNK(K)
1251.71771251.6196785455541(R) (R)
1346.75281346.6237965215300(K) (V)
1350.79081350.7646196306401(R) (D)
1852.08491851.9427771Met-ox 5635790(R)HGFNVGQTMFSLLVTGK(L)
1852.08491852.0009453123261(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (80/859AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 4/42 matches (9%).
Acc. #: Species: MOUSE Name: GPI-anchor transamidase (GPI transamidase) (Phosphatidylinositol-glycan biosynthesis, class K protein) (PIG-K)
Index: MW: 44954 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1707.94021707.9070192943081(R) (K)
1822.07221821.9322771551710(K) (F)
1838.10361837.9271961Met-ox 1551710(K)SNILIYMTGHGGNGFLK(F)
2225.33162225.2294461261451(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (52/395AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 5/42 matches (11%).
Acc. #: Species: HUMAN Name: TNF receptor associated factor 3 (CD40 receptor associated factor 1) (CRAF1) (CD40 binding protein) (CD40BP) (LMP1 associated protein) (LAP1) (CAP-1)
Index: MW: 64461 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1114.61461114.6294-131821910(K) (H)
1130.60781130.6244-151Met-ox 1821910(K)SQVPMIALQK(H)
1130.60781130.6421-303223311(R) (E)
1217.73351217.6490693773870(R) (H)
1350.79081350.6588983883980(R) (L)
1493.86761493.7568744945061(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (55/568AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 5/42 matches (11%).
Acc. #: Species: MOUSE Name: Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase) (GK)
Index: MW: 57458 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1114.61461114.5454622352440(R) (A)
1130.60781130.5404601Met-ox 2352440(R)SSSEIYGLMK(A)
1217.73351217.6642571301401(R) (T)
1383.80501383.7524381411530(K) (L)
2065.25652065.0905802933101(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (52/524AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 5/42 matches (11%).
Acc. #: Species: HUMAN Name: Chloride channel protein CLC-KA (ClC-K1)
Index: MW: 75285 Da pI: 7.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1114.61461114.6261-1031400(R) (Q)
1350.79081350.7745126756871(K) (-)
1838.10361838.0097511481650(K) (V)
1994.14881994.03615777921(R) (Y)
2211.32562211.1735691281471(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (77/687AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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