MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 2
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D79
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 2221 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/107(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
19.053e+0049 (8)21.08.4-5.1752.2 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
25.040e+00411 (10)30.010.31.2757.9 59556/5.4MOUSET-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
32.765e+00413 (12)20.012.1-4.4352.7 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
42.694e+0049 (8)17.08.42.7366.5 81278/6.1HUMANSEC14-like protein 1
52.358e+00410 (9)23.09.3-9.3453.4 59621/5.4HUMANT-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
61.089e+00410 (9)11.09.3-10.249.9 93056/5.4HUMANColorectal mutant cancer protein (MCC protein)
757775 (4)14.04.717.029.9 39220/6.2HUMANPyruvate dehydrogenase E1 component beta subunit, mitochondrial precursor (PDHE1-B)
855379 (8)26.08.4-18.539.2 51272/8.6HUMANImport inner membrane translocase subunit TIM44, mitochondrial precursor
949347 (6)13.06.5-17.568.5 86175/8.4HUMANZinc finger protein 184
1045266 (5)27.05.66.9545.6 37280/5.6MOUSECathepsin B precursor (Cathepsin B1)
45313......................x......x..x...........x....x.......x.x............x..............x...................
84429..............x..................x.....x..xx.................x.....x...........x.......x.....x..........x..
42717...............x......xx...............x.........xx..x.....x....x..x...xx......................x...........
26420.............................x.....................................x......x.xx......x...x....xx............
61980..............x....x......x......x........xx.................x.....x.................x.x...................
112583....x.................x..........xx..........x..........x............x.....x.........x.................x...
45078.......................................x..x........................x.................................x.x...
43510...........x................................x..................x.......x.............x..x.x..xx............
21481.............x....x....x......................x........x........................x...................x......
60091.....x.....................................x.....................x.x...x.................x.................


Detailed Results

1. 9/107 matches (8%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.56741179.6010-283773860(K) (H)
1277.65901277.6337202472571(K) (N)
1277.65901277.7105-404734830(K) (T)
1300.51021300.5302-152582670(K) (N)
1383.65421383.6909-261861970(K) (V)
1475.74411475.7494-3.62122230(K) (T)
1475.74411475.7858-282002110(R) (W)
1699.82171699.8251-2.0pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1716.85541716.85172.24184320(K) (G)
1994.01541993.9772192242390(R) (R)
2384.06332383.9524475195490(R) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 21% (140/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/107 matches (10%).
Acc. #: Species: MOUSE Name: T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
Index: MW: 59556 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.46901065.5217-493914000(R) (V)
1307.65541307.63841321310(K) (N)
1349.62351349.6887-481Met-ox 8201(K)APGFAQMLKDGAK(H)
1365.61761365.5956163793900(R) (A)
1381.61671381.5905191Met-ox 3793900(R)GSTDNLMDDIER(A)
1753.96731753.9812-7.94054212(R) (Q)
1851.95321851.90232738541(K) (M)
2230.22112230.1470334915091(K) (L)
2384.06332384.1114-202612811(K) (A)
2464.27762464.2295201822030(K) (V)
2785.48062785.4484124514762(R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (168/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 13/107 matches (12%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1078.45211078.4852-315735810(R) (A)
1179.56741179.55817.96776850(K) (F)
1201.54981201.54246.17647740(R) (C)
1349.62351349.6596-275976082(R) (K)
1475.74411475.6841411Met-ox 2882990(K)VHFLTDPENEMK(E)
1486.74461486.7766-226366501(K) (F)
1650.78471650.8022-117507632(R) (A)
1716.85541716.8267172883011(K) (L)
1791.77121791.8601-506776901(K) (R)
1851.95321851.9751-124214390(K) (G)
1992.04411992.04111.51171(-) (A)
1994.01541994.0500-1725410(R) (T)
2502.33152502.2371385425642(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 20% (165/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/107 matches (8%).
Acc. #: Species: HUMAN Name: SEC14-like protein 1
Index: MW: 81278 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1277.65901277.6054421361460(K) (I)
1851.95321851.8612501811980(R) (K)
2083.06582083.1089-215585752(K) (K)
2195.00062195.0993-455986151(K) (E)
2211.17332211.0942361Met-ox 5986151(K)VWQLGRDYSMVESPLICK(E)
2287.23372287.2709-163373562(K) (A)
2403.27072403.3189-204404592(R) (F)
2464.27762464.2481123844040(K) (H)
2480.21142480.2430-131Met-ox 3844040(K)VFGRPISSWTCLVDLEGLNMR(H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (126/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/107 matches (9%).
Acc. #: Species: HUMAN Name: T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Index: MW: 59621 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.46901065.5217-493914000(R) (V)
1158.54981158.5907-354674760(K) (N)
1232.57111232.6098-312973070(K) (Y)
1307.65541307.63841321310(K) (N)
1365.61761365.5956163793900(R) (A)
1381.61671381.5905191Met-ox 3793900(R)GSTDNLMDDIER(A)
1753.96731753.9812-7.94054212(R) (Q)
1851.95321851.90232738541(K) (M)
2298.20792298.2644-2555741(K) (E)
2384.06332384.1114-202612811(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (129/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 10/107 matches (9%).
Acc. #: Species: HUMAN Name: Colorectal mutant cancer protein (MCC protein)
Index: MW: 93056 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.4718973.5179-472682742(R) (R)
1179.56741179.6010-287687780(R) (A)
1307.65541307.6596-3.23934021(K) (L)
1323.61931323.6215-1.61791900(K) (H)
1384.65911384.7185-432842970(R) (L)
1677.78731677.8369-304124250(K) (S)
1940.92391940.9024117127280(R) (R)
2097.04892097.0035227127291(R) (E)
2298.20792298.20152.84074251(R) (S)
2768.50542768.4617164034252(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (95/829AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 5/107 matches (4%).
Acc. #: Species: HUMAN Name: Pyruvate dehydrogenase E1 component beta subunit, mitochondrial precursor (PDHE1-B)
Index: MW: 39220 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1349.62351349.61933.12592690(K) (T)
1365.61761365.61432.51Met-ox 2592690(K)EGVECEVINMR(T)
1851.95321851.9019282702850(R) (T)
2751.53632751.436436pyroGlu 2332580(R)QGTHITVVSHSRPVGHCLEAAAVLSK(E)
2768.50542768.4630152332580(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (53/359AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 9/107 matches (8%).
Acc. #: Species: HUMAN Name: Import inner membrane translocase subunit TIM44, mitochondrial precursor
Index: MW: 51272 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1051.50461051.5536-472092172(R) (F)
1383.65421383.6756-154034151(R) (V)
1790.79671790.8729-431152(-) (R)
1992.04411992.1130-352752932(R) (T)
2298.20792298.2431-151892082(K) (K)
2403.27072403.2966-113824020(K) (N)
2435.26822435.2865-7.52Met-ox 3824020(K)MMEQGPVLIITFQAQLVMVVR(N)
2464.27762464.2394152943142(K) (F)
2480.21142480.2343-9.21Met-ox 2943142(K)TEMSEVLTEILRVDPAFDKDR(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (118/452AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 7/107 matches (6%).
Acc. #: Species: HUMAN Name: Zinc finger protein 184
Index: MW: 86175 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1060.49351060.5427-462432510(R) (C)
1155.56981155.6122-371531620(R) (E)
1201.54981201.6040-452302390(K) (H)
1395.64421395.7021-415665770(K) (K)
1659.77871659.7987-125295412(K) (S)
2233.15362233.0836311862060(K) (R)
2739.42362739.3464281051290(R) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (100/751AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 6/107 matches (5%).
Acc. #: Species: MOUSE Name: Cathepsin B precursor (Cathepsin B1)
Index: MW: 37280 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
996.5046996.5076-3.02382450(K) (N)
1381.61671381.61054.52692810(K) (I)
1794.80251794.8550-2972870(R) (E)
1851.95321851.9023273153310(R) (T)
1992.04411991.9755342462630(K) (S)
2418.20262418.18427.637561(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 27% (92/339AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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