MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D80
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1060 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/96(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.180e+01224 (25)72.025.0-17.526.9 26560/5.4HUMANGlutathione S-transferase Mu 3 (GSTM3-3) (GST class-mu 3) (hGSTM3-3)
295459 (9)11.09.49.7361.6 56807/6.6HUMANSerine/threonine-protein kinase receptor R2 precursor (SKR2) (Activin receptor-like kinase 4) (ALK-4) (ACTR-IB)
342498 (8)14.08.3-11.356.9 70527/6.4MOUSEVacuolar protein sorting 33B
441057 (7)23.07.3-23.619.9 32819/4.7HUMANTropomyosin alpha 3 chain (Tropomyosin 3) (Tropomyosin gamma)
538778 (8)15.08.3-2.4551.6 73403/9.3HUMANZinc finger protein 133
114672..x.......x.xx.xx.......x..........x.x..x...x.......x.x.xx...x....x.......xx.xxx.....x.....x....
77311........x.............x................................x.........x.x.....x...x.x......x.........
112586.......................................x.............xx.x.....x..x.....xx.......................
23003..x.......x..........x...............................x.x.....................x.x................
24933..............x.......xx...........x..........................x..................x...x.......x..


Detailed Results

1. 24/96 matches (25%).
Acc. #: Species: HUMAN Name: Glutathione S-transferase Mu 3 (GSTM3-3) (GST class-mu 3) (hGSTM3-3)
Index: MW: 26560 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
875.4443875.4627-2148540(R) (F)
940.4227940.4602-40pyroGlu 1411480(K)QFSMFLGK(F)
956.4138956.4552-43pyroGlu 1Met-ox 1411480(K)QFSMFLGK(F)
957.4445957.4868-441411480(K) (F)
971.4535971.4627-9.51491560(K) (L)
973.4653973.4817-171Met-ox 1411480(K)QFSMFLGK(F)
1015.58561015.5900-4.474820(K) (Y)
1149.54831149.5614-111781860(K) (A)
1220.53811220.5556-141Met-ox 2162250(K)MAQWGNKPVC(-)
1257.70431257.7279-1972821(K) (Y)
1360.54491360.5480-2.337470(R) (S)
1457.68681457.7099-161982090(K) (M)
1478.71641478.7313-101011120(R) (T)
1494.71181494.7263-9.71Met-ox 1011120(R)VDIIENQVMDFR(T)
1516.65071516.64911.136471(K) (S)
1587.75291587.7794-1723350(R) (R)
1655.90221655.9372-211271400(K) (Q)
1743.85421743.8805-1523361(R) (Y)
1747.83021747.9165-49991121(K) (T)
1758.76911758.7831-8.0AcetN 1150(-)SCESSMVLGYWDIR(G)
1763.87421763.8750-0.491131261(R) (L)
1763.87421763.9114-211Met-ox 991121(K)IRVDIIENQVMDFR(T)
1774.75941774.7780-10AcetN 1Met-ox 1150(-)SCESSMVLGYWDIR(G)
1972.96491973.0020-191571720(K) (I)
2216.95792216.9923-1637541(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 72% (163/225AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/96 matches (9%).
Acc. #: Species: HUMAN Name: Serine/threonine-protein kinase receptor R2 precursor (SKR2) (Activin receptor-like kinase 4) (ALK-4) (ACTR-IB)
Index: MW: 56807 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
900.4547900.4105493Met-ox 4684741(R)VMGKMMR(E)
1009.46511009.4856-202402461(R) (E)
1490.72571490.7459-141Met-ox 3463590(K)NGMCAIADLGLAVR(H)
1653.73461653.7868-323803930(R) (H)
1669.74601669.7817-211Met-ox 3803930(R)YMAPEVLDETINMK(H)
1742.75471742.7109251621750(R) (D)
1758.76911758.7059361Met-ox 1621750(R)LDMEDPSCEMCLSK(D)
1774.75941774.7008332Met-ox 1621750(R)LDMEDPSCEMCLSK(D)
2041.89762041.833931pyroGlu 2Met-ox 1601751(R)QRLDMEDPSCEMCLSK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (58/505AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/96 matches (8%).
Acc. #: Species: MOUSE Name: Vacuolar protein sorting 33B
Index: MW: 70527 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1234.63641234.62935.83053141(K) (R)
1475.74041475.7456-3.57190(R) (R)
1478.71641478.6763273783890(R) (Q)
1494.71181494.7739-42911031(K) (L)
1601.79581601.8395-271Met-ox 3403530(R)LLSLHIGACESIMK(K)
1653.73461653.8025-412913040(R) (A)
1713.98121713.9396243403541(R) (K)
1715.88531715.9440-343994130(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (92/617AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/96 matches (7%).
Acc. #: Species: HUMAN Name: Tropomyosin alpha 3 chain (Tropomyosin 3) (Tropomyosin gamma)
Index: MW: 32819 Da pI: 4.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
875.4443875.4474-3.52062130(K) (Y)
940.4227940.4488-281531600(K) (K)
1003.44171003.4696-2822300(R) (Q)
1475.74041475.7780-251Met-ox 1411521(K)MELQEIQLKEAK(H)
1490.72571490.7525-1836481(R) (K)
1758.76911758.8220-302692840(K) (-)
1774.75941774.8169-321Met-ox 2692840(K)AISEELDHALNDMTSI(-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (66/284AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/96 matches (8%).
Acc. #: Species: HUMAN Name: Zinc finger protein 133
Index: MW: 73403 Da pI: 9.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
968.5246968.5278-3.23954020(R) (T)
968.5246968.5529-292832900(K) (T)
1009.46511009.46381.32742811(R) (K)
1013.48161013.4548261Met-ox 5475530(R)EKPYMCR(Q)
1149.54831149.6016-464744830(R) (H)
1601.79581601.7971-0.775265391(R) (H)
1790.82101790.8570-202352490(R) (G)
1972.96491972.9196235475621(R) (S)
2284.14232284.0807276346540(R) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (100/654AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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