MS-Fit Search Results

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D81
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1415 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/100(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	8.937e+010	14 (14)	51.0	14.0	-9.20	37.6	29192/5.7	HUMAN	Proteasome subunit beta type 4 precursor (Proteasome beta chain) (Macropain beta chain) (Multicatalytic endopeptidase complex beta chain) (Proteasome chain 3) (HSN3) (HsBPROS26)
2	1.397e+007	10 (10)	35.0	10.0	-12.8	45.9	29116/5.5	MOUSE	Proteasome subunit beta type 4 precursor (Proteasome beta chain) (Macropain beta chain) (Multicatalytic endopeptidase complex beta chain) (Proteasome chain 3)
3	1.070e+007	12 (12)	25.0	12.0	-6.44	55.2	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
4	2.054e+006	11 (11)	17.0	11.0	0.215	54.0	87299/9.4	MOUSE	Plakophilin 3
5	1.016e+006	9 (9)	26.0	9.0	5.73	65.8	41793/5.3	HUMAN	Actin, cytoplasmic 2 (Gamma-actin)

23555	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	x	.	.	.	x	.	.	.
43090	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	x	.	.	.	.	.	.	.
45313	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	x	.	.	x	x	.	.	.	.	.	.	x	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.
86951	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.
110418	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 14/100 matches (14%).
Acc. #: Species: HUMAN Name: Proteasome subunit beta type 4 precursor (Proteasome beta chain) (Macropain beta chain) (Multicatalytic endopeptidase complex beta chain) (Proteasome chain 3) (HSN3) (HsBPROS26)
Index: MW: 29192 Da pI: 5.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Database Sequence
983.4983	983.5427	-45		132	139	(R) (A)
1111.5389	1111.5748	-32	pyroGlu	202	211	(K)QPVLSQTEAR(D)
1128.5623	1128.6013	-35		202	211	(K) (D)
1547.7745	1547.8103	-23	1Met-ox	46	60	(R)TQNPMVTGTSVLGVK(F)
1867.8992	1867.8900	4.9		24	40	(R) (G)
2012.9885	2013.0115	-11		61	80	(K) (F)
2028.9831	2029.0065	-12	1Met-ox	61	80	(K)FEGGVVIAADMLGSYGSLAR(F)
2193.9792	2194.0127	-15		90	109	(R) (Q)
2210.0353	2210.0076	13	1Met-ox	90	109	(R)VNNSTMLGASGDYADFQYLK(Q)
2426.1803	2426.1662	5.8	pyroGlu	110	131	(K)QVLGQMVIDEELLGDGHSYSPR(A)
2442.1620	2442.1611	0.36	pyroGlu 1Met-ox	110	131	(K)QVLGQMVIDEELLGDGHSYSPR(A)
2443.2001	2443.1927	3.0		110	131	(K) (A)
2459.1920	2459.1877	1.8	1Met-ox	110	131	(K)QVLGQMVIDEELLGDGHSYSPR(A)
2648.2617	2648.2190	16	1Met-ox	241	264	(K)GVEIEGPLSTETNWDIAHMISGFE(-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 51% (136/264AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/100 matches (10%).
Acc. #: Species: MOUSE Name: Proteasome subunit beta type 4 precursor (Proteasome beta chain) (Macropain beta chain) (Multicatalytic endopeptidase complex beta chain) (Proteasome chain 3)
Index: MW: 29116 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
983.4983 983.5427 -45 132 139 0 (R) (A)
1111.5389 1111.5748 -32 pyroGlu 202 211 0 (K)QPVLSQTEAR(E)
1128.5623 1128.6013 -35 202 211 0 (K) (E)
1547.7745 1547.8103 -23 1Met-ox 46 60 0 (R)TQNPMVTGTSVLGVK(F)
1998.9367 1998.9959 -30 61 80 0 (K) (F)
2211.0478 2210.9916 25 1Met-ox 90 109 0 (R)VNDSTMLGASGDYADFQYLK(Q)
2426.1803 2426.1662 5.8 pyroGlu 110 131 0 (K)QVLGQMVIDEELLGDGHSYSPR(A)
2442.1620 2442.1611 0.36 pyroGlu 1Met-ox 110 131 0 (K)QVLGQMVIDEELLGDGHSYSPR(A)
2443.2001 2443.1927 3.0 110 131 0 (K) (A)
2459.1920 2459.1877 1.8 1Met-ox 110 131 0 (K)QVLGQMVIDEELLGDGHSYSPR(A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (95/264AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/100 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
999.4893 999.4457 44 290 298 0 (K) (V)
999.4893 999.4972 -7.9 387 395 1 (R) (I)
1015.4823 1015.4406 41 1Met-ox 290 298 0 (K)DVDGAYMTK(V)
1125.5244 1125.5428 -16 367 376 0 (K) (Y)
1141.5461 1141.5199 23 464 472 0 (R) (L)
1179.5618 1179.6010 -33 377 386 0 (K) (H)
1302.6426 1302.7018 -45 393 403 1 (R) (V)
1383.6669 1383.6909 -17 186 197 0 (K) (V)
1475.7345 1475.7494 -10 212 223 0 (K) (T)
1475.7345 1475.7858 -35 200 211 0 (R) (W)
1687.9149 1687.9707 -33 66 82 1 (R) (G)
1993.9622 1993.9772 -7.5 224 239 0 (R) (R)
2383.9772 2383.9524 10 519 549 0 (R) (G)
2400.2060 2400.2121 -2.5 464 483 1 (R) (T)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
999.4893	999.4457	44		290	298	0	(K) (V)
999.4893	999.4972	-7.9		387	395	1	(R) (I)
1015.4823	1015.4406	41	1Met-ox	290	298	0	(K)DVDGAYMTK(V)
1125.5244	1125.5428	-16		367	376	0	(K) (Y)
1141.5461	1141.5199	23		464	472	0	(R) (L)
1179.5618	1179.6010	-33		377	386	0	(K) (H)
1302.6426	1302.7018	-45		393	403	1	(R) (V)
1383.6669	1383.6909	-17		186	197	0	(K) (V)
1475.7345	1475.7494	-10		212	223	0	(K) (T)
1475.7345	1475.7858	-35		200	211	0	(R) (W)
1687.9149	1687.9707	-33		66	82	1	(R) (G)
1993.9622	1993.9772	-7.5		224	239	0	(R) (R)
2383.9772	2383.9524	10		519	549	0	(R) (G)
2400.2060	2400.2121	-2.5		464	483	1	(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (166/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 11/100 matches (11%).
Acc. #: Species: MOUSE Name: Plakophilin 3
Index: MW: 87299 Da pI: 9.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1005.4670 1005.4754 -8.3 299 307 0 (R) (T)
1111.5389 1111.5788 -36 738 746 0 (R) (K)
1350.6486 1350.6905 -31 174 185 0 (R) (L)
1499.7026 1499.6701 22 82 94 0 (R) (S)
1515.7121 1515.6651 31 1Met-ox 82 94 0 (R)GQYHTMQTGFSSR(S)
1783.8832 1783.9343 -29 422 437 0 (K) (D)
1940.8911 1940.8860 2.7 78 94 1 (R) (S)
2193.9792 2193.9844 -2.4 1Met-ox 556 575 1 (R)RDMAGAPPGEMVGCFTPQSR(R)
2193.9792 2193.9844 -2.4 1Met-ox 557 576 1 (R)DMAGAPPGEMVGCFTPQSRR(L)
2210.0353 2209.9793 25 2Met-ox 556 575 1 (R)RDMAGAPPGEMVGCFTPQSR(R)
2210.0353 2209.9793 25 2Met-ox 557 576 1 (R)DMAGAPPGEMVGCFTPQSRR(L)
2210.0353 2210.1358 -46 103 122 0 (K) (S)
2231.1627 2231.1308 14 174 194 1 (R) (Y)
2400.2060 2400.1180 37 218 241 1 (R) (T)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1005.4670	1005.4754	-8.3		299	307	0	(R) (T)
1111.5389	1111.5788	-36		738	746	0	(R) (K)
1350.6486	1350.6905	-31		174	185	0	(R) (L)
1499.7026	1499.6701	22		82	94	0	(R) (S)
1515.7121	1515.6651	31	1Met-ox	82	94	0	(R)GQYHTMQTGFSSR(S)
1783.8832	1783.9343	-29		422	437	0	(K) (D)
1940.8911	1940.8860	2.7		78	94	1	(R) (S)
2193.9792	2193.9844	-2.4	1Met-ox	556	575	1	(R)RDMAGAPPGEMVGCFTPQSR(R)
2193.9792	2193.9844	-2.4	1Met-ox	557	576	1	(R)DMAGAPPGEMVGCFTPQSRR(L)
2210.0353	2209.9793	25	2Met-ox	556	575	1	(R)RDMAGAPPGEMVGCFTPQSR(R)
2210.0353	2209.9793	25	2Met-ox	557	576	1	(R)DMAGAPPGEMVGCFTPQSRR(L)
2210.0353	2210.1358	-46		103	122	0	(K) (S)
2231.1627	2231.1308	14		174	194	1	(R) (Y)
2400.2060	2400.1180	37		218	241	1	(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (137/797AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/100 matches (9%).
Acc. #: Species: HUMAN Name: Actin, cytoplasmic 2 (Gamma-actin)
Index: MW: 41793 Da pI: 5.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1132.4936 1132.5275 -30 197 206 0 (R) (E)
1198.6641 1198.7061 -35 29 39 0 (R) (H)
1499.7026 1499.6767 17 pyroGlu 360 372 0 (K)QEYDESGPSIVHR(K)
1515.7121 1515.7497 -25 85 95 0 (K) (V)
1516.6928 1516.7032 -6.8 360 372 0 (K) (K)
1790.8894 1790.8925 -1.7 239 254 0 (K) (F)
1980.9950 1980.9081 44 1 18 0 (-) (A)
2012.9885 2012.8979 45 2Met-ox 1 18 0 (-)MEEEIAALVIDNGSGMCK(A)
2231.1627 2231.0654 44 1Met-ox 292 312 0 (K)DLYANTVLSGGTTMYPGIADR(M)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Database Sequence
1132.4936	1132.5275	-30		197	206	(R) (E)
1198.6641	1198.7061	-35		29	39	(R) (H)
1499.7026	1499.6767	17	pyroGlu	360	372	(K)QEYDESGPSIVHR(K)
1515.7121	1515.7497	-25		85	95	(K) (V)
1516.6928	1516.7032	-6.8		360	372	(K) (K)
1790.8894	1790.8925	-1.7		239	254	(K) (F)
1980.9950	1980.9081	44		1	18	(-) (A)
2012.9885	2012.8979	45	2Met-ox	1	18	(-)MEEEIAALVIDNGSGMCK(A)
2231.1627	2231.0654	44	1Met-ox	292	312	(K)DLYANTVLSGGTTMYPGIADR(M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (100/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):