MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D82
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 170 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/69(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	8.584e+004	10 (14)	46.0	14.5	-3.13	29.7		24428/5.7		HUMAN	26S proteasome non-ATPase regulatory subunit 10 (26S proteasome regulatory subunit p28) (Gankyrin)
2	1504	6 (8)	32.0	8.7	-3.24	33.3		25054/5.7		MOUSE	26S proteasome non-ATPase regulatory subunit 10 (26S proteasome regulatory subunit p28) (Gankyrin)
3	1095	4 (5)	15.0	5.8	-11.7	59.1		59610/8.5		HUMAN	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (6PF-2-K/Fru-2,6-P2ASE brain/placenta-type isozyme) (iPFK-2) [Includes: 6-phosphofructo-2-kinase ; Fructose-2,6-bisphosphatase ]
4	841	5 (7)	6.0	7.2	-12.5	92.2		98602/8.7		MOUSE	ADAM 12 precursor (A disintegrin and metalloproteinase domain 12) (Meltrin alpha)
5	825	5 (7)	13.0	7.2	4.35	46.1		44728/8.3		HUMAN	Phosphoglycerate kinase 1 (Primer recognition protein 2) (PRP 2)

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98267	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	x	.	x	.	.	.	.	.
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98673	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 10/69 matches (14%).
Acc. #: Species: HUMAN Name: 26S proteasome non-ATPase regulatory subunit 10 (26S proteasome regulatory subunit p28) (Gankyrin)
Index: MW: 24428 Da pI: 5.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
926.6204	926.6151	5.7		214	222	1	(K) (M)
1193.6645	1193.6757	-9.3		154	162	0	(K) (A)
1209.6688	1209.6706	-1.4	1Met-ox	154	162	0	(K)MIHILLYYK(A)
1621.8862	1621.9140	-17	1Met-ox	150	162	1	(K)GNLKMIHILLYYK(A)
1921.0591	1921.0282	16		190	206	1	(K) (T)
2035.0704	2035.0395	15		117	135	1	(K) (D)
2051.0158	2051.0344	-9.1	1Met-ox	117	135	1	(K)NRHEIAVMLLEGGANPDAK(D)
2208.0329	2208.1049	-33		70	90	1	(K) (A)
2485.1177	2485.1265	-3.5		163	184	0	(K) (V)
3041.4273	3041.4122	5.0		163	189	1	(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 46% (106/226AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/69 matches (8%).
Acc. #: Species: MOUSE Name: 26S proteasome non-ATPase regulatory subunit 10 (26S proteasome regulatory subunit p28) (Gankyrin)
Index: MW: 25054 Da pI: 5.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
926.6204 926.6151 5.7 214 222 1 (K) (L)
2035.0704 2035.0395 15 117 135 1 (K) (D)
2051.0158 2051.0344 -9.1 1Met-ox 117 135 1 (K)NRHEIAVMLLEGGANPDAK(D)
2208.0329 2208.1049 -33 70 90 1 (K) (A)
2485.1177 2485.1265 -3.5 163 184 0 (K) (V)
3041.4273 3041.4122 5.0 163 189 1 (K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (76/231AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/69 matches (5%).
Acc. #: Species: HUMAN Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (6PF-2-K/Fru-2,6-P2ASE brain/placenta-type isozyme) (iPFK-2) [Includes: 6-phosphofructo-2-kinase ; Fructose-2,6-bisphosphatase ]
Index: MW: 59610 Da pI: 8.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
2105.0068 2105.0450 -18 429 445 1 (R) (S)
2211.1384 2211.1994 -28 26 46 1 (R) (G)
2238.1308 2238.0600 32 320 338 0 (K) (D)
2485.1177 2485.1995 -33 148 169 0 (K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (79/520AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/69 matches (7%).
Acc. #: Species: MOUSE Name: ADAM 12 precursor (A disintegrin and metalloproteinase domain 12) (Meltrin alpha)
Index: MW: 98602 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1151.5849 1151.6424 -50 239 248 0 (R) (F)
1179.5875 1179.6374 -42 52 63 0 (K) (D)
1621.8862 1621.8199 41 755 770 0 (R) (G)
2211.1384 2211.2402 -46 239 256 1 (R) (I)
2658.5697 2658.4772 35 249 270 1 (K) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 6% (60/903AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/69 matches (7%).
Acc. #: Species: HUMAN Name: Phosphoglycerate kinase 1 (Primer recognition protein 2) (PRP 2)
Index: MW: 44728 Da pI: 8.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
926.6204 926.6039 18 40 48 1 (R) (F)
1206.6776 1206.6404 31 1Met-ox 351 361 1 (R)GTKALMDEVVK(A)
2035.0704 2035.0469 12 57 75 0 (K) (Y)
2051.0158 2051.0418 -13 1Met-ox 57 75 0 (K)SVVLMSHLGRPDGVPMPDK(Y)
2105.0068 2105.0609 -26 333 350 0 (K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (57/418AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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