MS-Fit Search Results


Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D83
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (10000 - 40000 Da) selects 57907 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 5763 entries.
Pre searches select 5763 entries.

Data Set 1 Results

MS-Fit search selects 12 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/36(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.623e+00812 (33)56.033.3-7.2421.3 30540/5.9HUMANPeroxiredoxin 4 (Prx-IV) (Thioredoxin peroxidase AO372) (Thioredoxin-dependent peroxide reductase A0372) (Antioxidant enzyme AOE372) (AOE37-2)
21.425e+0058 (22)36.022.2-8.9924.8 31053/6.7MOUSEPeroxiredoxin 4 (Prx-IV) (Thioredoxin peroxidase AO372) (Thioredoxin-dependent peroxide reductase A0372) (Antioxidant enzyme AOE372)
35495 (13)29.013.9-6.6972.1 25022/5.5HUMANL-3-phosphoserine phosphatase (PSP) (O-phosphoserine phosphohydrolase) (PSPase)
43374 (11)36.011.1-36.355.4 14209/6.6HUMANFATTY ACID-BINDING PROTEIN, LIVER (L-FABP)
51134 (11)33.011.126.880.5 14120/9.5MOUSEINSULIN-LIKE GROWTH FACTOR IA PRECURSOR (IGF-IA) (SOMATOMEDIN)
675.94 (11)21.011.114.2102 27978/5.9MOUSEDEAD-box RNA helicase DEAD2 (mDEAD2) (D1PAS1 related sequence 1)
731.24 (11)14.011.1-40.1105 34155/9.9MOUSEMitochondrial 2-oxoglutarate/malate carrier protein (OGCP)
818.95 (13)13.013.927.131.9 34819/8.7MOUSETranscription initiation factor IIB (General transcription factor TFIIB) (RNA polymerase II alpha initiation factor)
918.95 (13)13.013.927.131.9 34833/8.7HUMANTranscription initiation factor IIB (General transcription factor TFIIB) (S300-II)
1013.04 (11)21.011.1-38.574.2 28380/9.2HUMANTranscription initiation factor IIF, beta subunit (TFIIF-beta) (Transcription initiation factor RAP30)
12999xx......x.xx....x...x...x.x.....xx.x
95660xx......x.xx........x...........x..x
80420.....x.......xx...x.........x.......
13297...x.........x.x.................x..
29927.xx.............x..x................
18186.....xx......xx.....................
108686x....x.....x...x....................
36055........x..x..x.....x.x.............
96521........x..x..x.....x.x.............
42336............xx.....x...x............


Detailed Results

1. 12/36 matches (33%).
Acc. #: Species: HUMAN Name: Peroxiredoxin 4 (Prx-IV) (Thioredoxin peroxidase AO372) (Thioredoxin-dependent peroxide reductase A0372) (Antioxidant enzyme AOE372) (AOE37-2)
Index: MW: 30540 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
920.4864920.5093-252012080(R) (G)
953.5411953.5645-251651731(R) (I)
1208.65221208.6639-9.7pyroGlu 2132230(R)QITLNDLPVGR(S)
1212.60961212.6265-142312400(R) (H)
1225.67831225.6905-10.02132230(R) (S)
1464.83641464.8426-4.21741860(R) (D)
1624.76241624.76071.11872000(K) (G)
2108.04592108.0704-1281990(K) (E)
2199.94332199.94061.348660(R) (V)
2417.19112417.1924-0.532412630(K) (L)
2457.08842457.0893-0.3746661(R) (V)
2859.44032859.4100111401640(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 56% (153/271AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/36 matches (22%).
Acc. #: Species: MOUSE Name: Peroxiredoxin 4 (Prx-IV) (Thioredoxin peroxidase AO372) (Thioredoxin-dependent peroxide reductase A0372) (Antioxidant enzyme AOE372)
Index: MW: 31053 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
920.4864920.5093-252042110(R) (G)
953.5411953.5645-251681761(R) (I)
1208.65221208.6639-9.7pyroGlu 2162260(R)QITLNDLPVGR(S)
1212.60961212.6265-142342430(R) (H)
1225.67831225.6905-10.02162260(R) (S)
1624.76241624.76071.11902030(K) (G)
2417.19112417.1924-0.532442660(K) (L)
2859.44032859.4100111431670(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (100/274AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/36 matches (13%).
Acc. #: Species: HUMAN Name: L-3-phosphoserine phosphatase (PSP) (O-phosphoserine phosphohydrolase) (PSPase)
Index: MW: 25022 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1116.51741116.5836-591Met-ox 191(-)MISHSELRK(L)
1329.71871329.7279-7.01231340(K) (L)
1336.72721336.7377-7.91021130(R) (S)
1541.87781541.8804-1.779920(R) (E)
2284.19932284.1025422072251(K) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (66/225AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/36 matches (11%).
Acc. #: Species: HUMAN Name: FATTY ACID-BINDING PROTEIN, LIVER (L-FABP)
Index: MW: 14209 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1102.51701102.5744-5281900(K) (L)
1329.71871329.7378-1479901(K) (L)
1386.66611386.7593-6734461(K) (H)
2457.08842457.1165-1158780(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (46/127AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/36 matches (11%).
Acc. #: Species: MOUSE Name: INSULIN-LIKE GROWTH FACTOR IA PRECURSOR (IGF-IA) (SOMATOMEDIN)
Index: MW: 14120 Da pI: 9.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
953.5411953.4805641181261(R) (M)
1085.51361085.5414-261001081(R) (E)
1464.83641464.75955379900(R) (A)
1580.74621580.72011660720(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (43/127AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 4/36 matches (11%).
Acc. #: Species: MOUSE Name: DEAD-box RNA helicase DEAD2 (mDEAD2) (D1PAS1 related sequence 1)
Index: MW: 27978 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1116.51741116.5941-691361440(K) (R)
1163.60071163.59088.51251350(R) (V)
1329.71871329.6664391982081(R) (K)
1329.71871329.6837261Met-ox 80901(R)RIVEQDTMPPK(G)
1336.72721336.63946669790(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (53/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 4/36 matches (11%).
Acc. #: Species: MOUSE Name: Mitochondrial 2-oxoglutarate/malate carrier protein (OGCP)
Index: MW: 34155 Da pI: 9.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
920.4864920.45113849570(R) (T)
1116.51741116.5836-5912211(R) (S)
1225.67831225.7632-692612711(K) (Y)
1386.66611386.7633-701091220(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (44/314AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 5/36 matches (13%).
Acc. #: Species: MOUSE Name: Transcription initiation factor IIB (General transcription factor TFIIB) (RNA polymerase II alpha initiation factor)
Index: MW: 34819 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1208.65221208.621026pyroGlu 2272370(K)QVQMAATHIAR(K)
1225.67831225.6475252272370(K) (K)
1336.72721336.71608.4pyroGlu 2272381(K)QVQMAATHIARK(A)
1624.76241624.6769533Met-ox 1061191(R)TMSSSDRAMMNAFK(E)
1871.95041871.9061241Met-ox 2012170(K)ALETSVDLITTGDFMSR(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (43/316AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 5/36 matches (13%).
Acc. #: Species: HUMAN Name: Transcription initiation factor IIB (General transcription factor TFIIB) (S300-II)
Index: MW: 34833 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1208.65221208.621026pyroGlu 2272370(K)QVQMAATHIAR(K)
1225.67831225.6475252272370(K) (K)
1336.72721336.71608.4pyroGlu 2272381(K)QVQMAATHIARK(A)
1624.76241624.6769533Met-ox 1061191(R)TMSSSDRAMMNAFK(E)
1871.95041871.9061241Met-ox 2012170(K)ALETSVDLITTGDFMSR(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (43/316AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 4/36 matches (11%).
Acc. #: Species: HUMAN Name: Transcription initiation factor IIF, beta subunit (TFIIF-beta) (Transcription initiation factor RAP30)
Index: MW: 28380 Da pI: 9.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1321.76571321.75924.92192301(K) (N)
1329.71871329.8146-722082181(K) (E)
1580.74621580.7783-201Met-ox 1811930(K)QHVLDMLFSAFEK(H)
2098.01162098.1508-661301471(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (54/249AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.