MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D85
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 672 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/87(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.607e+0059 (10)53.010.3-19.120.1 25022/5.5HUMANL-3-phosphoserine phosphatase (PSP) (O-phosphoserine phosphohydrolase) (PSPase)
28.148e+00411 (12)23.012.6-29.2105 73141/6.7MOUSEPutative ATP-dependent RNA helicase PL10
36.393e+0048 (9)17.09.2-47.661.3 59556/5.4MOUSET-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
46.153e+00410 (11)21.011.5-31.9120 73095/7.0HUMANDEAD-box protein 3, Y-chromosomal
53.597e+0048 (9)18.09.2-51.363.1 59621/5.4HUMANT-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
62.524e+0048 (9)19.09.2-46.957.6 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
71.170e+0047 (8)17.08.0-38.894.0 58868/5.2MOUSEProto-oncogene tyrosine-protein kinase FGR (P55-FGR) (C-FGR)
81.011e+0049 (10)19.010.3-28.1119 73102/6.7MOUSEDEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2)
91.009e+0049 (10)19.010.3-28.1119 73244/6.7HUMANDEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
1085848 (9)21.09.2-46.0101 68206/8.6HUMANZinc finger protein 23 (Zinc finger protein KOX16) (DKFZp569D2231)
80420....................................................xx.xx........x....x.x....x.......x.
98092......................x.........x.........x........xxx.....xxx.x............x..........
84429..............................x..........x.......x...........xx............x..xx.......
26939....x...............x.....................x........xxx.x...x...x............x..........
61980..............................x..........x.......x............x.......x....x..xx.......
45313......................x....................x..........x...x.....x..x.......x..x........
89314.....................x.....................x............x.......x.....x..x......x......
65272.............x............................x........xxx.....x.x.x............x..........
3370.............x............................x........xxx.....x.x.x............x..........
6922x..............................................x.............x........xx.xxx...........


Detailed Results

1. 9/87 matches (10%).
Acc. #: Species: HUMAN Name: L-3-phosphoserine phosphatase (PSP) (O-phosphoserine phosphohydrolase) (PSPase)
Index: MW: 25022 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1329.69561329.7279-241231340(K) (L)
1336.70781336.7377-221021130(R) (S)
1496.64501496.6725-181Met-ox 37490(K)ICGVEDAVSEMTR(R)
1541.85001541.8804-2079920(R) (E)
2091.96732092.0061-1910270(K) (E)
2220.03192220.1011-319271(R) (E)
2284.11212284.10254.22072251(K) (-)
2414.97682415.0417-271371580(K) (G)
3076.43813076.4825-1410361(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 53% (120/225AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/87 matches (12%).
Acc. #: Species: MOUSE Name: Putative ATP-dependent RNA helicase PL10
Index: MW: 73141 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
999.3694999.4383-691011090(R) (G)
1074.50221074.5472-424184260(K) (R)
1163.58801163.5908-2.44074170(R) (V)
1320.60531320.6800-572762860(R) (K)
1329.69561329.5972742Met-ox 3763860(R)HTMMFSATFPK(E)
1329.69561329.68379.01Met-ox 3623721(R)RIVEQDTMPPK(G)
1336.70781336.6394513513610(R) (R)
1707.73361707.8964-953113251(R) (L)
1765.68881765.7741-485996151(R) (A)
1838.88151838.9513-385315471(R) (N)
1940.89031940.9792-463353501(K) (M)
2399.03372399.2433-873874061(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (158/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/87 matches (9%).
Acc. #: Species: MOUSE Name: T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
Index: MW: 59556 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1052.42011052.4835-6045540(R) (M)
1150.57851150.5897-9.74414500(K) (A)
1307.58091307.6384-4421310(K) (N)
1838.88151838.9574-411211370(R) (K)
1851.88561851.9023-9.038541(K) (M)
2383.90132384.1114-882612811(K) (A)
2416.00652416.1012-392Met-ox 2612811(K)TAEELMNFSKGEENLMDAQVK(A)
2457.05812457.2773-892392601(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (98/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/87 matches (11%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3, Y-chromosomal
Index: MW: 73095 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
912.2947912.3811-956216300(R) (G)
996.4227996.4387-161011090(R) (E)
1163.58801163.5908-2.44064160(R) (V)
1320.60531320.6800-572752850(R) (K)
1329.69561329.5972742Met-ox 3753850(R)HTMMFSATFPK(E)
1329.69561329.68379.01Met-ox 3613711(R)RIVEQDTMPPK(G)
1336.70781336.6394513503600(R) (R)
1496.64501496.7749-875335460(R) (N)
1707.73361707.8964-953103241(R) (L)
1940.89031940.9792-463343491(K) (M)
2399.03372399.2433-873864051(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (139/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/87 matches (9%).
Acc. #: Species: HUMAN Name: T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Index: MW: 59621 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1052.42011052.4835-6045540(R) (M)
1150.57851150.5897-9.74414500(K) (A)
1307.58091307.6384-4421310(K) (N)
1851.88561851.9023-9.038541(K) (M)
2220.03192220.1885-712042241(R) (K)
2383.90132384.1114-882612811(K) (A)
2416.00652416.1012-392Met-ox 2612811(K)TAEELMNFSKGEENLMDAQVK(A)
2457.05812457.2773-892392601(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (102/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/87 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
999.3694999.4457-762902980(K) (V)
1179.49761179.6010-883773860(K) (H)
1475.70941475.7494-272122230(K) (T)
1475.70941475.7858-522002110(R) (W)
1638.80101638.8604-361861991(K) (F)
1993.95401993.9772-122242390(R) (R)
2150.02012150.0783-272242401(R) (R)
2383.90132383.9524-215195490(R) (G)
2416.00652416.2070-831Met-ox 4644831(R)DYQELMNTKLALDLEIATYR(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (125/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 7/87 matches (8%).
Acc. #: Species: MOUSE Name: Proto-oncogene tyrosine-protein kinase FGR (P55-FGR) (C-FGR)
Index: MW: 58868 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
997.4532997.4842-3180880(R) (G)
997.4532997.5219-691801871(R) (I)
1179.49761179.6084-942902990(K) (L)
1541.85001541.7811451601731(R) (D)
1993.95401993.919717921060(K) (S)
2220.03192220.1772-652802991(K) (L)
2342.96262343.1014-592562751(R) (V)
2584.13042584.2692-542572791(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (90/517AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 9/87 matches (10%).
Acc. #: Species: MOUSE Name: DEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2)
Index: MW: 73102 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
940.3166940.4012-9056640(K) (D)
1163.58801163.5908-2.44084180(R) (V)
1320.60531320.6800-572772870(R) (K)
1329.69561329.5972742Met-ox 3773870(R)HTMMFSATFPK(E)
1329.69561329.68379.01Met-ox 3633731(R)RIVEQDTMPPK(G)
1336.70781336.6394513523620(R) (R)
1707.73361707.8964-953123261(R) (L)
1838.88151838.9513-385325481(R) (N)
1940.89031940.9792-463363511(K) (M)
2399.03372399.2433-873884071(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (132/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 9/87 matches (10%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
940.3166940.4012-9056640(K) (D)
1163.58801163.5908-2.44084180(R) (V)
1320.60531320.6800-572772870(R) (K)
1329.69561329.5972742Met-ox 3773870(R)HTMMFSATFPK(E)
1329.69561329.68379.01Met-ox 3633731(R)RIVEQDTMPPK(G)
1336.70781336.6394513523620(R) (R)
1707.73361707.8964-953123261(R) (L)
1838.88151838.9513-385325481(R) (N)
1940.89031940.9792-463363511(K) (M)
2399.03372399.2433-873884071(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (132/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 8/87 matches (9%).
Acc. #: Species: HUMAN Name: Zinc finger protein 23 (Zinc finger protein KOX16) (DKFZp569D2231)
Index: MW: 68206 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
900.3354900.32659.91Met-ox 4854900(K)CMECEK(A)
1257.45421257.5794-1005655740(K) (A)
1838.88151839.0129-711061201(K) (C)
2220.03192220.0950-285755931(K) (S)
2283.14872283.0511434174341(R) (C)
2342.96262343.0974-583043221(R) (G)
2368.96122369.1270-703363551(R) (G)
2383.90132384.1240-933884061(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (126/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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