MS-Fit Search Results



.

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D91
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1874 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score
#/96(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.014e+00817 (17)58.017.7-66.741.1 28723/5.8HUMANProteasome activator complex subunit 1 (Proteasome activator 28-alpha subunit) (PA28alpha) (PA28a) (Activator of multicatalytic protease subunit 1) (11S regulator complex alpha subunit) (REG-alpha) (Interferon gamma up-regulated I-5111 protein) (IGUP I>
21.182e+00716 (16)28.016.7-51.953.1 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
32.560e+00615 (15)21.015.6-55.150.7 59519/5.1HUMANKeratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
47.973e+00513 (13)44.013.5-68.640.5 28673/5.7MOUSEProteasome activator complex subunit 1 (Proteasome activator 28-alpha subunit) (PA28alpha) (PA28a) (Activator of multicatalytic protease subunit 1) (11S regulator complex alpha subunit) (REG-alpha)
51.696e+00516 (16)21.016.7-61.165.1 90956/8.1HUMANVasopressin-activated calcium-mobilizing receptor (VACM-1) (Cullin homolog 5) (CUL-5)
61.249e+00512 (12)17.012.5-42.369.8 89278/6.8HUMANTFIIH basal transcription factor complex helicase XPB subunit (Basic transcription factor 2 89 kDa subunit) (BTF2-p89) (TFIIH 89 kDa subunit) (DNA-repair protein complementing XP-B cells) (Xeroderma pigmentosum group B complementing protein) (DNA excis>
77.921e+0048 (8)10.08.3-54.083.5 96866/5.3HUMANHeat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
86.049e+00414 (14)14.014.6-42.466.7 93056/5.4HUMANColorectal mutant cancer protein (MCC protein)
94.181e+0048 (8)13.08.3-45.074.5 91801/4.7HUMANFetal alzheimer antigen (Fetal Alz-50-reactive clone 1)
102.652e+00411 (11)11.011.5-26.381.4 96985/6.9HUMANPhosphatidylinositol 4-kinase alpha (PI4-kinase) (PtdIns-4-kinase) (PI4K-alpha)
43291...x...xx.............xx......xx...x..x......xx........x..x..xx.........................x...x...
45313.x................xx.x..x.......x.x.x...x....x........x..........x.....x..........x......x.x....
108237.........x.....xx...........x.....x.x..xx.x.x....x......x............x...x..........x...........
92029...x...xx.............xx......xx............x.x..............xx.........................x...x...
106859...x...x.........x.....x...xx.x........x......x..xx.....x...............x......x....x........x..
17954.......................xxxx...x....x..x...........................x..x............xx......x.....
27666x..x......x......................x....x...............x...x...............................x.....
112583.....................x..x...x.x........xx..x...........x..........x.x..x........x...xx..........
82762........x......x........x....x......x..x.......................................x..........x.....
29828...x.....x...............x....x..x.....xx..xx................x..x...............................



Detailed Results

1. 17/96 matches (17%).
Acc. #: Species: HUMAN Name: Proteasome activator complex subunit 1 (Proteasome activator 28-alpha subunit) (PA28alpha) (PA28a) (Activator of multicatalytic protease subunit 1) (11S regulator complex alpha subunit) (REG-alpha) (Interferon gamma up-regulated I-5111 protein) (IGUP I>
Index: MW: 28723 Da pI: 5.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
923.3895923.4694-872Met-ox 2142200(R)LMVMEIR(N)
954.3742954.4434-72pyroGlu 1911980(K)QPHVGDYR(Q)
971.3968971.4699-751911980(K) (Q)
1159.52651159.6111-731671760(K) (Y)
1163.56471163.6676-8836451(K) (E)
1260.63651260.7204-6759700(K) (E)
1268.56861268.6527-6625350(K) (K)
1305.60561305.6877-631561660(K) (L)
1321.59511321.6826-661Met-ox 1561660(K)VFELMTSLHTK(L)
1383.61081383.7418-95AcetN 1131(-)AMLRVQPEAQAK(V)
1395.64971395.6942-3214241(K) (T)
1395.64971395.7888-1002212320(R) (N)
1484.62531484.7021-52pyroGlu 1992100(R)QLVHELDEAEYR(D)
1501.64761501.7287-541992100(R) (D)
1517.75901517.8579-6559721(K) (E)
1519.67541519.7393-421421550(R) (V)
2284.06152284.2449-801Met-ox 2142321(R)LMVMEIRNAYAVLYDIILK(N)
2429.23642429.2928-2337581(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 58% (146/249AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/96 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
909.3890909.4543-72AcetN 181(-)SRQFSSR(S)
1125.44611125.5428-863673760(K) (Y)
1127.45291127.5407-782892981(K) (V)
1157.50791157.5149-6.01Met-ox 4644720(R)DYQELMNTK(L)
1179.51791179.6010-703773860(K) (H)
1277.61461277.6337-152472571(K) (N)
1277.61461277.7105-754734830(K) (T)
1300.48961300.5302-312582670(K) (N)
1316.53821316.52519.91Met-ox 2582670(K)NMQDMVEDYR(N)
1340.58441340.6698-643653761(K) (Y)
1383.61081383.6909-581861970(K) (V)
1475.66961475.7494-542122230(K) (T)
1475.66961475.7858-792002110(R) (W)
1539.71461539.7841-451Met-ox 4614721(R)LLRDYQELMNTK(L)
1687.88281687.9707-5266821(R) (G)
1993.86111993.9772-582242390(R) (R)
2285.98742286.1254-603673861(K) (H)
2383.85412383.9524-415195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (184/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 15/96 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
993.4907993.5005-9.92382450(K) (Q)
1090.43891090.5315-851481560(K) (L)
1109.40071109.4904-813353430(K) (S)
1234.59271234.6796-702362451(R) (Q)
1300.48961300.5956-812862951(K) (N)
1316.53821316.5905-401Met-ox 2862951(K)NHEEEMKDLR(N)
1324.58321324.6174-263353451(K) (E)
1340.58441340.6923-80pyroGlu 2462571(R)QSVEADINGLRR(V)
1365.54941365.6399-663233330(R) (K)
1381.60511381.6487-321661770(R) (I)
1434.65271434.7705-824404501(K) (S)
1493.67091493.7348-433233341(R) (D)
1622.79241622.8277-221661791(R) (E)
1707.68851707.7727-4941590(K) (G)
2096.85702096.9810-594234390(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (125/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/96 matches (13%).
Acc. #: Species: MOUSE Name: Proteasome activator complex subunit 1 (Proteasome activator 28-alpha subunit) (PA28alpha) (PA28a) (Activator of multicatalytic protease subunit 1) (11S regulator complex alpha subunit) (REG-alpha)
Index: MW: 28673 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
923.3895923.4694-872Met-ox 2142200(R)LMVMEIR(N)
954.3742954.4434-72pyroGlu 1911980(K)QPHVGDYR(Q)
971.3968971.4699-751911980(K) (Q)
1159.52651159.6111-731671760(K) (Y)
1163.56471163.6676-8836451(K) (E)
1260.63651260.7204-6759700(K) (E)
1268.56861268.6527-6625350(K) (K)
1381.60511381.6786-5314241(K) (T)
1395.64971395.7888-1002212320(R) (N)
1517.75901517.8579-6559721(K) (E)
1519.67541519.7393-421421550(R) (V)
2284.06152284.2449-801Met-ox 2142321(R)LMVMEIRNAYAVLYDIILK(N)
2429.23642429.2928-2337581(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 44% (110/249AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 16/96 matches (16%).
Acc. #: Species: HUMAN Name: Vasopressin-activated calcium-mobilizing receptor (VACM-1) (Cullin homolog 5) (CUL-5)
Index: MW: 90956 Da pI: 8.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
923.3895923.4474-6311180(K) (W)
954.3742954.4533-832052120(K) (F)
1111.48431111.5359-461Met-ox 4484551(K)DVFMRYHK(A)
1163.56471163.6207-481Met-ox 6927001(R)LQLTTERMR(E)
1222.46351222.5778-944824920(R) (L)
1234.59271234.6328-332933020(K) (V)
1260.63651260.7051-544254351(K) (E)
1324.58321324.6901-817557640(K) (Y)
1395.64971395.7636-823403500(K) (F)
1434.65271434.6099301Met-ox 7697800(R)DESDINTFIYMA(-)
1439.65231439.7898-963723841(K) (L)
1493.67091493.7820-742Met-ox 7157261(R)TQEAIIQIMKMR(K)
1698.81311698.9642-897297430(K) (N)
1851.83881851.8985-321Met-ox 2883021(R)NETEKLHLMFSLMDK(V)
2096.85702097.0439-891Met-ox 1381541(K)LMLDTWNESIFSNIKNR(L)
2557.29462557.4064-447297501(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (167/780AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 12/96 matches (12%).
Acc. #: Species: HUMAN Name: TFIIH basal transcription factor complex helicase XPB subunit (Basic transcription factor 2 89 kDa subunit) (BTF2-p89) (TFIIH 89 kDa subunit) (DNA-repair protein complementing XP-B cells) (Xeroderma pigmentosum group B complementing protein) (DNA excis>
Index: MW: 89278 Da pI: 6.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1163.56471163.56182.51Met-ox 2742820(K)QEMIEELQK(R)
1163.56471163.6247-5260681(K) (D)
1179.51791179.6196-861Met-ox 60681(K)DYRLQMPLK(D)
1203.53491203.6050-586796880(R) (V)
1215.55961215.5646-4.153621(K) (L)
1260.63651260.7217-687687781(K) (R)
1305.60561305.6877-631461570(K) (L)
1321.59511321.6826-661Met-ox 1461570(K)TGVPDGIMQFIK(L)
1541.66731541.6728-3.61Met-ox 3803910(K)MWSTIDDSQICR(F)
1622.79241622.7986-3.82182341(K) (V)
1993.86111993.85632.42422570(K) (D)
2056.84162056.9802-674885040(K) (V)
2342.96252343.1567-832012221(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (137/782AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 8/96 matches (8%).
Acc. #: Species: HUMAN Name: Heat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
Index: MW: 96866 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
907.3815907.3831-1.78518580(K) (-)
923.3895923.3780121Met-ox 8518580(K)NSVNMDLD(-)
1009.39661009.4777-807157220(K) (L)
1290.57781290.6945-9085950(K) (N)
1321.59511321.7156-912222340(K) (N)
1475.66961475.7342-443613740(K) (G)
1501.64761501.7862-923323460(K) (I)
2342.96252343.0671-452762950(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (89/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 14/96 matches (14%).
Acc. #: Species: HUMAN Name: Colorectal mutant cancer protein (MCC protein)
Index: MW: 93056 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1157.50791157.6101-881Met-ox 8018091(R)HETQVRMLK(Q)
1179.51791179.6010-707687780(R) (A)
1234.59271234.7047-916346431(K) (K)
1260.63651260.6986-495085191(K) (L)
1324.58321324.6063-177908000(K) (H)
1340.58441340.6013-131Met-ox 7908000(K)LESQMMAMVER(H)
1372.53351372.5911-423Met-ox 7908000(K)LESQMMAMVER(H)
1484.62531484.6911-442Met-ox 7898001(K)KLESQMMAMVER(H)
1541.66731541.6827-10.01Met-ox 2402520(R)EENESLTAMLCSK(E)
1601.74291601.7892-291Met-ox 53651(K)LAKAQCEQSHLMR(E)
1687.88281687.8325306967110(K) (T)
1940.83071940.9024-377127280(R) (R)
2096.85702097.0035-707127291(R) (E)
2180.91532181.0531-631Met-ox 2342521(K)ELAGLREENESLTAMLCSK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (120/829AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/96 matches (8%).
Acc. #: Species: HUMAN Name: Fetal alzheimer antigen (Fetal Alz-50-reactive clone 1)
Index: MW: 91801 Da pI: 4.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
971.3968971.4699-757527600(K) (V)
1090.43891090.4904-474814900(K) (G)
1179.51791179.51294.24404491(K) (D)
1179.51791179.5394-1857670(R) (R)
1253.57261253.5538154564650(R) (D)
1316.53821316.6156-593914010(R) (A)
1324.58321324.6610-596536650(K) (L)
1851.83881852.0108-9377921(R) (S)
2342.96252343.1331-731731920(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (110/810AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 11/96 matches (11%).
Acc. #: Species: HUMAN Name: Phosphatidylinositol 4-kinase alpha (PI4-kinase) (PtdIns-4-kinase) (PI4K-alpha)
Index: MW: 96985 Da pI: 6.9
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
923.3895923.4661-838218280(R) (V)
993.4907993.4675231Met-ox 5435520(K)SGTPMQSAAK(A)
993.4907993.5039-131Met-ox 79880(R)SMSLNIGGAK(G)
1203.53491203.5970-523163260(K) (L)
1260.63651260.6087221621711(K) (Y)
1290.57781290.6013-181Met-ox 8118201(K)HRFSPNMTER(E)
1324.58321324.6315-367487590(K) (M)
1340.58441340.6264-311Met-ox 7487590(K)LTDEMVMIMGGK(M)
1372.53351372.6162-603Met-ox 7487590(K)LTDEMVMIMGGK(M)
1381.60511381.7123-781Met-ox 891011(K)GSMNRHVAAIGPR(F)
1517.75901517.6881473150(R) (F)
1533.62751533.6830-361Met-ox 3150(R)EMAGAWHMTVEQK(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (97/854AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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