MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D92
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1756 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/86(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.362e+00513 (15)23.015.1-49.664.0 73529/5.9MOUSEStress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (P66 MOT) (Mortalin)
21.360e+00513 (15)23.015.1-49.664.0 73681/5.9HUMANStress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (Mortalin) (MOT)
35.466e+00413 (15)20.015.1-25.587.8 74210/6.5MOUSELamin A
44.621e+00414 (16)20.016.3-29.167.3 83935/6.4HUMANDystrobrevin alpha (Dystrobrevin-alpha)
53.135e+0046 (6)15.07.023.5101 77888/8.7HUMANZinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
63.040e+00411 (12)13.012.8-7.4594.0 85888/6.7HUMANMyb-related protein A (A-Myb)
72.857e+0045 (5)20.05.830.6116 38273/5.7MOUSES-adenosylmethionine decarboxylase proenzyme 1 (AdoMetDC 1) (SamdC 1) [Contains: S-adenosylmethionine decarboxylase 1 alpha chain; S-adenosylmethionine decarboxylase 1 beta chain]
82.469e+0049 (10)16.010.5-20.858.7 86175/8.4HUMANZinc finger protein 184
92.332e+0046 (6)27.07.0-54.863.4 34538/5.7MOUSEFollistatin-related protein 1 precursor (TGF-beta-inducible protein TSC-36)
102.113e+0047 (8)15.08.1-13.552.0 59519/5.1HUMANKeratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
31570................x....x.................xxxx........x.....x...x......xx.x.....x........
55501................x....x.................xxxx........x.....x...x......xx.x.....x........
14097.x.....x..........x...x.....x..x......x.....x.........xx.x..x......................x..
3064.....x.x.......................x.....x.xx.x.x.....xx.xx..x................x...........
46452.............................................x..................x..........xxx.x......
108603.x........x.....xx....................x..x......x.x............x...........x.....x....
35175...................................x...................xx..................x........x.
21481...........x.....................x............x...xx.x.............x.........x..x.....
78412...................x.......x...........x.................x........x..x................
108237......................xx..............x..............x...x............xx..............


Detailed Results

1. 13/86 matches (15%).
Acc. #: Species: MOUSE Name: Stress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (P66 MOT) (Mortalin)
Index: MW: 73529 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.49571045.5392-421Met-ox 6476541(K)LFEMAYKK(M)
1149.52471149.5540-251271351(R) (D)
1444.59361444.7106-816016121(K) (E)
1446.65721446.7626-733783910(K) (M)
1450.59341450.7178-8686990(R) (L)
1462.75751462.7576-0.0441Met-ox 3783910(K)SDIGEVILVGGMTR(M)
1608.72751608.7692-261Met-ox 1741871(K)MKETAENYLGHTAK(N)
1707.78811707.8455-34pyroGlu 1081221(R)QAVTNPNNTFYATKR(L)
1812.84371812.9756-731601751(K) (E)
2055.90262055.9623-292662840(K) (H)
2091.83532092.0419-9953730(K) (Q)
2096.92182097.0691-70pyroGlu 6356531(R)QAASSLQQASLKLFEMAYK(K)
2233.08902233.1060-7.66266461(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (161/679AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/86 matches (15%).
Acc. #: Species: HUMAN Name: Stress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (Mortalin) (MOT)
Index: MW: 73681 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.49571045.5392-421Met-ox 6476541(K)LFEMAYKK(M)
1149.52471149.5540-251271351(R) (D)
1444.59361444.7106-816016121(K) (E)
1446.65721446.7626-733783910(K) (M)
1450.59341450.7178-8686990(R) (L)
1462.75751462.7576-0.0441Met-ox 3783910(K)SDIGEVILVGGMTR(M)
1608.72751608.7692-261Met-ox 1741871(K)MKETAENYLGHTAK(N)
1707.78811707.8455-34pyroGlu 1081221(R)QAVTNPNNTFYATKR(L)
1812.84371812.9756-731601751(K) (E)
2055.90262055.9623-292662840(K) (H)
2091.83532092.0419-9953730(K) (Q)
2096.92182097.0691-70pyroGlu 6356531(R)QAASSLQQASLKLFEMAYK(K)
2233.08902233.1060-7.66266461(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (161/679AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 13/86 matches (15%).
Acc. #: Species: MOUSE Name: Lamin A
Index: MW: 74210 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
919.3588919.4386-874284350(R) (T)
951.4710951.46486.52342401(K) (L)
1119.49151119.5897-881451550(K) (R)
1165.57181165.54892079890(K) (K)
1320.59771320.6409-334284391(R) (V)
1350.63701350.6225115165270(K) (T)
1434.68681434.6721105875990(R) (A)
1475.69341475.7681-511Met-ox 3673781(K)LALDMEIHAYRK(L)
1676.82141676.8608-242362491(R) (A)
1687.94641687.8727449251(R) (I)
1707.78811707.8050-9.94214351(K) (T)
1794.77101794.8485-431Met-ox 4724861(R)QNGDDPLMTYRFPPK(F)
2383.97612384.1846-872412601(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (137/665AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 14/86 matches (16%).
Acc. #: Species: HUMAN Name: Dystrobrevin alpha (Dystrobrevin-alpha)
Index: MW: 83935 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
937.3954937.4413-491Met-ox 5325390(R)MSALQESR(R)
951.4710951.4570151Met-ox 181(-)MIEDSGKR(G)
1350.63701350.6541-137327430(K) (-)
1420.67231420.7549-583894011(K) (L)
1444.59361444.6920-683653771(R) (L)
1446.65721446.7813-865405511(R) (L)
1462.75751462.7762-131Met-ox 5405511(R)RELMVQLEGLMK(L)
1475.69341475.6562252742870(R) (E)
1606.78051606.8012-136776910(R) (G)
1606.78051606.8077-177307431(K) (-)
1608.72751608.7804-331Met-ox 17291(R)QLFAEMRAQDLDR(I)
1608.72751608.8379-692993131(K) (E)
1622.81491622.7961121Met-ox 6776910(R)KPGYIHSGATTSTMR(G)
1676.82141676.9331-672Met-ox 5415541(R)ELMVQLEGLMKLLK(T)
1707.78811707.7972-5.31Met-ox 5265391(K)DELEQRMSALQESR(R)
2198.90942198.9689-276346530(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (154/743AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/86 matches (6%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1519.66321519.7691-705335450(K) (V)
1949.03541948.9299543663821(K) (M)
2211.09262211.07567.71431610(K) (T)
2225.11172225.0304376066240(R) (K)
2233.08902232.9225752402570(K) (V)
2284.15822284.0715384714891(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (105/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 11/86 matches (12%).
Acc. #: Species: HUMAN Name: Myb-related protein A (A-Myb)
Index: MW: 85888 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
919.3588919.4485-986796860(K) (T)
993.6076993.6097-2.15185260(K) (E)
1045.49571045.4638311781850(K) (R)
1103.51141103.5737-566266340(K) (E)
1434.68681434.6899-2.17237340(K) (R)
1450.59341450.6848-631Met-ox 7237340(K)TEDQLIMTEQAR(R)
1590.86521590.7910477237351(K) (Y)
1606.78051606.7859-3.41Met-ox 7237351(K)TEDQLIMTEQARR(Y)
1851.90251851.89454.32Met-ox 2702841(K)ELEMLLMSAENEVRR(K)
2211.09262211.07567.77057221(K) (T)
2298.12702298.0043531Met-ox 6356540(K)EESGTQLLTEDISDMQSENR(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (100/752AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 5/86 matches (5%).
Acc. #: Species: MOUSE Name: S-adenosylmethionine decarboxylase proenzyme 1 (AdoMetDC 1) (SamdC 1) [Contains: S-adenosylmethionine decarboxylase 1 alpha chain; S-adenosylmethionine decarboxylase 1 beta chain]
Index: MW: 38273 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1396.55781396.6207-451120(-) (L)
1687.94641687.788993pyroGlu 60740(K)QEAYVLSESSMFVSK(R)
1704.91271704.81555760740(K) (R)
2211.09262210.9527631521680(R) (V)
2705.20762705.2492-151291510(R) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (67/334AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 9/86 matches (10%).
Acc. #: Species: HUMAN Name: Zinc finger protein 184
Index: MW: 86175 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1014.50431014.5332-285425501(R) (I)
1385.63381385.5942293083181(K) (T)
1525.67021525.7512-534264380(K) (T)
1606.78051606.8566-475946061(R) (I)
1608.72751608.8134-537067180(K) (I)
1622.81491622.8226-4.72302421(K) (T)
2050.95692051.0285-353473631(R) (C)
2233.08902233.08362.41862060(K) (R)
2294.06392294.05593.55485651(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (125/751AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/86 matches (6%).
Acc. #: Species: MOUSE Name: Follistatin-related protein 1 precursor (TGF-beta-inducible protein TSC-36)
Index: MW: 34538 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1125.50371125.5428-351401490(K) (Y)
1252.49061252.5931-8228380(K) (E)
1444.59361444.7259-922192301(K) (C)
1707.78811707.8124-141011160(K) (D)
2045.00942045.0748-321231390(R) (G)
2091.83532091.9909-741932100(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (84/306AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/86 matches (8%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1165.57181165.5853-124424500(R) (S)
1185.54941185.5235225775890(K) (G)
1434.68681434.7705-584404501(K) (S)
1622.81491622.8277-7.91661791(R) (E)
1707.78811707.77279.041590(K) (G)
2096.00682096.0473-192672840(K) (K)
2096.92182096.9810-284234390(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (92/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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