MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D93
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (0 - 100000 Da) selects 107150 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12217 entries.
Pre searches select 12217 entries.

Data Set 1 Results

MS-Fit search selects 1165 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/64(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16.976e+00611 (17)34.017.24.2035.5 28994/6.8HUMANEndoplasmic reticulum protein ERp29 precursor (ERp31) (ERp28)
21.231e+00610 (15)25.015.611.354.5 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
33.048e+0057 (10)38.010.95.3333.6 27547/5.7HUMAN6-phosphogluconolactonase (6PGL)
41.126e+00510 (15)12.015.618.869.4 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
54.321e+0046 (9)10.09.4-12.958.7 42189/8.7MOUSEHypothetical protein PP2447 homolog
64.308e+0046 (9)10.09.4-12.958.7 42321/8.2HUMANHypothetical protein PP2447
71.109e+0046 (9)15.09.417.497.7 77888/8.7HUMANZinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
81.092e+0047 (10)9.010.9-43.834.6 90764/6.7HUMANSodium/hydrogen exchanger 1 (Na(+)/H(+) exchanger 1) (NHE-1) (Na+/H+ antiporter, amiloride-sensitive) (APNH)
993668 (12)20.012.520.611.6 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
10907410 (15)24.015.6-7.8784.4 59621/5.4HUMANT-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
101427...x..x.x....x.....xx..............x.x..x.x...........x.........
45313....x.....x.....xx.....x.....x........x..x......x...........x...
81694............x..x......x........x..x.........x.............x.....
42717..........x........x......x..x..x...x....x...x.xx...............
42012........x............................x.xx.x....x................
86008........x............................x.xx.x....x................
46452...........................x.....................x.xxx.x........
39677.......x......x...........xx..x.........x.x.....................
97443......xx..........x..x.......................x.x..........x..x..
61980.x....x...........x..xx.............x.......x..x.........x..x...


Detailed Results

1. 11/64 matches (17%).
Acc. #: Species: HUMAN Name: Endoplasmic reticulum protein ERp29 precursor (ERp31) (ERp28)
Index: MW: 28994 Da pI: 6.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
937.4846937.47836.61982040(K) (I)
1065.53171065.5733-391972041(K) (I)
1134.63461134.6523-162442530(K) (K)
1247.57501247.55841360690(K) (Q)
1320.68201320.6628141131220(K) (D)
1324.71531324.71530.007237480(K) (V)
1608.77531608.7546131231370(R) (V)
1676.91241676.8688261101221(K) (D)
1708.80131708.78529.42092230(K) (I)
1724.80341724.7801141Met-ox 2092230(K)ILDQGEDFPASEMTR(I)
2233.13572233.12534.61231431(R) (W)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 34% (89/261AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/64 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1014.55271014.4791731Met-ox 181(-)MSRQFSSR(S)
1179.61871179.6010153773860(K) (H)
1300.55711300.5302212582670(K) (N)
1302.67591302.7018-203934031(R) (V)
1302.67591302.7157-313443550(R) (A)
1383.70241383.69098.31861970(K) (V)
1475.76201475.74948.52122230(K) (T)
1475.76201475.7858-162002110(R) (W)
1687.96991687.9707-0.4666821(R) (G)
1716.89101716.8517234184320(K) (G)
1994.02171993.9772222242390(R) (R)
2384.02862383.9524325195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 25% (166/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/64 matches (10%).
Acc. #: Species: HUMAN Name: 6-phosphogluconolactonase (6PGL)
Index: MW: 27547 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1197.57161197.53633073810(R) (L)
1267.75051267.7738-181861970(R) (T)
1365.67121365.66256.32362460(K) (L)
1490.86431490.83312157720(R) (W)
1601.91681601.90159.51711850(K) (V)
1767.87101767.87060.2282960(R) (T)
2299.16842299.1934-112152350(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (99/258AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/64 matches (15%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.61871179.5581516776850(K) (F)
1320.68201320.6548217507611(R) (C)
1459.77451459.6891582882990(K) (E)
1475.76201475.6841531Met-ox 2882990(K)VHFLTDPENEMK(E)
1493.74331493.6921346226320(K) (E)
1665.86261665.8634-0.481Met-ox 2142281(K)MLRSLESGSNFAPLK(V)
1716.89101716.8267372883011(K) (L)
1791.77411791.8601-486776901(K) (R)
1851.96631851.9751-4.84214390(K) (G)
1994.02171994.0500-1425410(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 12% (102/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/64 matches (9%).
Acc. #: Species: MOUSE Name: Hypothetical protein PP2447 homolog
Index: MW: 42189 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1134.63461134.63123.01992080(R) (V)
1676.91241676.8902131391530(R) (V)
1707.79981707.8484-28pyroGlu 3Met-ox 1391530(R)QNGLMSGLMQMLLLK(V)
1708.80131708.8800-462Met-ox 1391530(R)QNGLMSGLMQMLLLK(V)
1724.80341724.8749-413Met-ox 1391530(R)QNGLMSGLMQMLLLK(V)
1851.96631851.9256223353500(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (41/376AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 6/64 matches (9%).
Acc. #: Species: HUMAN Name: Hypothetical protein PP2447
Index: MW: 42321 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1134.63461134.63123.02002090(R) (V)
1676.91241676.8902131401540(R) (V)
1707.79981707.8484-28pyroGlu 3Met-ox 1401540(R)QNGLMSGLMQMLLLK(V)
1708.80131708.8800-462Met-ox 1401540(R)QNGLMSGLMQMLLLK(V)
1724.80341724.8749-413Met-ox 1401540(R)QNGLMSGLMQMLLLK(V)
1851.96631851.9256223363510(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (41/376AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 6/64 matches (9%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1460.76181460.7246253703820(K) (M)
2083.02242083.0936-342072241(R) (C)
2195.03142195.1395-494284451(R) (C)
2211.14712211.0756321431610(K) (T)
2225.17792225.0304666066240(R) (K)
2284.21702284.0715644714891(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (106/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 7/64 matches (10%).
Acc. #: Species: HUMAN Name: Sodium/hydrogen exchanger 1 (Na(+)/H(+) exchanger 1) (NHE-1) (Na+/H+ antiporter, amiloride-sensitive) (APNH)
Index: MW: 90764 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1097.52221097.5526-285140(R) (I)
1252.61481252.6690-434484580(K) (G)
1459.77451459.8677-644264380(R) (F)
1460.76181460.7902-195685791(R) (M)
1487.74931487.8011-35881000(K) (T)
1708.80131708.9056-615976120(K) (S)
1724.80341724.9005-561Met-ox 5976120(K)IPSAVSTVSMQNIHPK(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (75/815AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/64 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.53171065.4999301541620(K) (L)
1097.52221097.5088124784891(R) (G)
1307.70911307.6782242402491(R) (Q)
1350.70991350.6878164724861(K) (G)
1791.77411791.7283264905120(R) (G)
1851.96631851.9275213213351(K) (N)
2299.16842299.1202212712891(K) (N)
2705.20382705.16171663940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (126/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 10/64 matches (15%).
Acc. #: Species: HUMAN Name: T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Index: MW: 59621 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
924.4507924.4977-511Met-ox 3083140(K)YNIMLVR(L)
1065.53171065.52179.43914000(R) (V)
1307.70911307.63845421310(K) (N)
1350.70991350.7568-353083181(K) (W)
1365.67121365.5956553793900(R) (A)
1665.86261665.9151-31AcetN 1Met-ox 1161(-)ALHVPKAPGFAQMLK(E)
1767.87101767.9250-311381521(R) (N)
1851.96631851.90233538541(K) (M)
2298.14992298.2644-5055741(K) (E)
2384.02862384.1114-352612811(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (133/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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